Biological control of Sclerotinia sclerotiorum in lettuce by Fusarium lateritium

1984 ◽  
Vol 24 (125) ◽  
pp. 272 ◽  
Author(s):  
K Sitepu ◽  
HR Wallace
Keyword(s):  
Biological Control ◽  
Organic Matter ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Soil Surface ◽  
Subsequent Infection ◽  
Fusarium Lateritium ◽  
Ascospore Germination ◽  
Agar Media ◽  
Inhibition Zones

Fusarium lateritium inhibited ascospore germination and mycelial growth of Sclerotinia sclerotiorum in various agar media. Although the inhibition zones produced by F. lateritium were most extensive at 20�C, they were still substantial at 5 and 30�C. Sclerotinia sclerotiorum requires organic matter on the soil surface for subsequent infection of lettuce stems in pots. The introduction of F. lateritium into such pots, 7 h before introducing S. sclerotiorum, gave protection. In infected pots with organic matter, 99% of plants were infected after 6 d, whereas only 5% were infected when F. lateritium was introduced. After 14 d these values increased to 100 and 33%, respectively.

scholarly journals The effects of ground cover mowing height on the fauna of ground-dwelling arthropods in olive grove

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Kristijan Franin ◽  
Nikolina Kapić ◽  
Šime Marcelić ◽  
Zoran Šikić
Keyword(s):  
Biological Control ◽  
Organic Matter ◽  
Vegetation Cover ◽  
Ground Cover ◽  
Soil Surface ◽  
Soil Disturbance ◽  
Olive Grove ◽  
Experimental Site ◽  
Herbaceous Vegetation ◽  
Number Of Individuals

Ground dwelling insects, spiders, and other arthropods play an important role in agroecosystems as predators and saprophytic organisms. Their presence on the soil surface helps the biological control of pests, enhances organic matter decomposition and promotes biodiversity. Soil disturbance, particularly tilling and mowing has greatly affected the assemblage and abundance of epigeic arthropods. This study aimed to determine the effects of herbaceous vegetation cover mowing height on arthropods abundance and structure in olive orchards. The experimental site was divided into three zones (Z1, Z2, and Z3) regarding mowing heights (0 cm, 10 cm, and 15 cm ). This research was done in 2019 from the beginning of May untill the end of September. During this research, 1490 individuals were recorded belonging to 6 classes, 11 orders and 13 families. The number of individuals was higher in the zones Z2 and Z3 comparing with Z1. The highest number of individuals showed woodlices (Isopoda) comprising 67, 5% of all individuals collected. There were significant differences in the number of Carabidae, Bleteliidae and Forficulidae between the zones of research. Unlike Forficulidae, higher heights of mown positively affected the population of Carabidae and Blateliidae.  

Comparing the Fungicide Sensitivity of Sclerotinia sclerotiorum Using Mycelial Growth and Ascospore Germination Assays

Plant Disease ◽  
2021 ◽  
Author(s):  
Miller da Silva Lehner ◽  
Kaique Alves ◽  
Emerson Medeiros Del Ponte ◽  
Sarah Jane Pethybridge
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Mode Of Action ◽  
Mycelial Growth ◽  
White Mold ◽  
Fungicide Sensitivity ◽  
Snap Bean ◽  
Thiophanate Methyl ◽  
Ascospore Germination ◽  
Sensitivity Tests

The infection of the floral tissues of snap bean and other crops by Sclerotinia sclerotiorum, the causative agent of white mold, is by ascospores. Irrespective of the fungicide mode of action being evaluated, in vitro fungicide sensitivity tests are conducted almost exclusively using mycelial growth assays. This is likely due to difficulties and time involved in sclerotial conditioning required to produce apothecia and ascospores. The objective of this research was to compare estimates of fungicide sensitivity between mycelial growth and ascospore germination assays for S. sclerotiorum. Sensitivity assays were conducted using serial doses of three fungicides commonly used to control white mold: boscalid, fluazinam, and thiophanate-methyl. A total of 27 isolates were evaluated in replicated trials conducted for each fungicide and assay type. The effective concentration to reduce mycelial growth or ascospore germination by 50% (EC50) was estimated for each isolate, fungicide, assay type, and trial. The median EC50 values obtained from ascospore germination assays were 52.7, 10.0, and 2.7 times higher than those estimated from the mycelial growth for boscalid, fluazinam, and thiophanate-methyl, respectively. No significant correlation was found between EC50 values estimated by the two methods. These findings highlight differences that may be important in evaluating the sensitivity of S. sclerotiorum given the fungicide mode of action and how they will be used in the field.

Hypoxylon mammatum ascospore germination and mycelial growth on bark and wood media from young branches of trembling aspen

1976 ◽  
Vol 54 (13) ◽  
pp. 1438-1442 ◽  
Author(s):  
J. R. French ◽  
P. D. Manion
Keyword(s):  
Seasonal Variation ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Nutrient Source ◽  
Wood Tissue ◽  
Inhibiting Factors ◽  
Ascospore Germination ◽  
Agar Media ◽  
Growth Promoting ◽  
Hypoxylon Mammatum

Hypoxylon mammatum radial growth rates increased with increasing concentrations of propylene-oxide-sterilized bark or wood tissue from fourth-season branches as sole nutrient source in agar media. Assay of bark and wood of one- to three-season-old branches demonstrated clonal and seasonal variation in growth-promoting and spore-germination-inhibiting factors.

Interactions between Sclerotinia sclerotiorum and Epicoccum purpurascens

1991 ◽  
Vol 69 (11) ◽  
pp. 2503-2510 ◽  
Author(s):  
Ting Zhou ◽  
R. D. Reeleder ◽  
S. A. Sparace
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Germ Tube ◽  
Hyphal Growth ◽  
White Mold ◽  
Antifungal Compounds ◽  
Sterile Culture ◽  
Pod Yield ◽  
Culture Filtrates ◽  
Ascospore Germination

Sterile culture filtrates of Epicoccum purpurascens decreased severity of white mold of bean and increased pod yield when applied prior to inoculation of beans with Sclerotinia sclerotiorum. Extracts of these culture filtrates inhibited ascospore germination and mycelial growth of S. sclerotiorum. Similar inhibition of hyphal growth of S. sclerotiorum occurred when dual cultured with E. purpurascens. Contact and penetration of hyphae of S. sclerotiorum by hyphae of E. purpurascens occurred rarely on agar-covered slides and was not observed on flower petals. Inhibition of ascospore germination and germ-tube elongation occurred on agar-covered slides where E. purpurascens had been grown previously; however, addition of nutrients decreased inhibition. Colonization of bean flowers by E. purpurascens reduced white mold incidence. The degree of disease control was affected by addition of nutrients and whether or not colonized flowers were autoclaved. Key words: antifungal compounds, competition, biological control, white mold, Phaseolus.

scholarly journals Antagonism of saprobe fungi from semiarid areas of the Northeast of Brazil against Sclerotinia sclerotiorum and biocontrol of soybean white mold

2020 ◽  
Vol 41 (6) ◽  
pp. 2597-2612
Author(s):  
Douglas Casaroto Peitl ◽  
◽  
Ciro Hideki Sumida ◽  
Ricardo Marcelo Gonçalves ◽  
Sérgio Florentino Pascholati ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Antagonistic Activity ◽  
White Mold ◽  
Saprobe Fungi ◽  
Progress Curve ◽  
Ascospore Germination ◽  
Semiarid Areas ◽  
Soybean Plants ◽  
Commercial Fungicide

The antagonistic activity of 25 saprobe fungi from semiarid areas of Northeast Brazil was evaluated against Sclerotinia sclerotiorum (Lib.) de Bary (Helotiales: Sclerotiniaceae). Four fungi [Myrothecium sp. Tode (Hypocreales: Stachybotryaceae) isolate 2, Volutella minima Höhn. (Hypocreales: Nectriaceae), Phialomyces macrosporus P.C. Misra & P.H.B. Talbot (Pezizomycotina) and Dictyosporium tetraseriale Goh, Yanna & K.D. Hyde (Pleosporales: Dictyosporiaceae)] were selected and further tested their ability to inhibit mycelial growth, sclerotia formation and ascospore germination of S. sclerotiorum and to control white mold on soybean plants. V. minima and P. macrosporus filtrates at 50% effectively suppressed mycelial growth and Myrothecium sp. isolate 2 completely suppressed sclerotia formation and inhibited ascospore germination by over 95%, the same result as commercial fungicide fluazinam. Soybean plants pre-treated with Myrothecium sp. isolate 2, P. macrosporus, and V. minima and inoculated with S. sclerotiorum showed a reduction of 55.8%, 79.7%, and 83.2% of area under disease progress curve (AUDPC) of white mold, respectively, in relation to water. Collectively, these results underline the antagonistic activity of V. minima, P. macrosporus, and Myrothecium sp. isolate 2 against S. sclerotiorum and their potential as biocontrol agents of soybean white mold.

scholarly journals Streptomyces felleus YJ1: Potential Biocontrol Agents Against the Sclerotinia Stem Rot (Sclerotinia sclerotiorum) of Oilseed Rape

2014 ◽  
Vol 6 (4) ◽  
pp. 91 ◽  
Author(s):  
Guanglong Cheng ◽  
Yun Huang ◽  
Hui Yang ◽  
Fan Liu
Keyword(s):  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Inhibition Zone ◽  
Stem Rot ◽  
Dual Culture ◽  
Sclerotinia Stem Rot ◽  
Fermentation Liquid ◽  
Ascospore Germination ◽  
Biocontrol Potential

In order to explore the biocontrol potential of Streptomyces felleus YJ1 to sclerotinia stem rot of oilseed rape, we evaluated the effects of YJ1 as antagonistic strain on Sclerotinia sclerotiorum, including germination and formation of sclerotia, ascospore germination, mycelial growth and YJ1 colonization ability. We found the fermentation filtrate of YJ1 could inhibit sclerotia and ascospore germination and mycelial growth. In dual culture the inhibition zone diameter of YJ1 against S. sclerotiorum was 11.0 mm, and the inhibition rate reached to 80.26%. The ascospores germination was also significantly inhibited by YJ1 fermentation filtrate. In addition, YJ1 could colonize stably in rhizosphere and roots of rape. Otherwise, in the greenhouse we found the lesion would become smaller and slighter if the inoculated leaves were pretreated with YJ1 fermentation liquid. Therefore, our results strongly suggested that YJ1 was a promising biocontrol agent for control of oilseed rape sclerotinia stem rot.

scholarly journals Effect of Digestate and Straw Combined Application on Maintaining Rice Production and Paddy Environment

2021 ◽  
Vol 18 (11) ◽  
pp. 5714
Author(s):  
Xue Hu ◽  
Hongyi Liu ◽  
Chengyu Xu ◽  
Xiaomin Huang ◽  
Min Jiang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Surface Water ◽  
Soil Organic Matter ◽  
Paddy Soil ◽  
Rice Yield ◽  
Soil Surface ◽  
Rice Production ◽  
Straw Decomposition ◽  
Rice Grains ◽  
Combined Application

Few studies have focused on the combined application of digestate and straw and its feasibility in rice production. Therefore, we conducted a two-year field experiment, including six treatments: without nutrients and straw (Control), digestate (D), digestate + fertilizer (DF), digestate + straw (DS), digestate + fertilizer + straw (DFS) and conventional fertilizer + straw (CS), to clarify the responses of rice growth and paddy soil nutrients to different straw and fertilizer combinations. Our results showed that digestate and straw combined application (i.e., treatment DFS) increased rice yield by 2.71 t ha−1 compared with the Control, and digestate combined with straw addition could distribute more nitrogen (N) to rice grains. Our results also showed that the straw decomposition rate at 0 cm depth under DS was 5% to 102% higher than that under CS. Activities of catalase, urease, sucrase and phosphatase at maturity under DS were all higher than that under both Control and CS. In addition, soil organic matter (SOM) and total nitrogen (TN) under DS and DFS were 20~26% and 11~12% higher than that under B and DF respectively, suggesting straw addition could benefit paddy soil quality. Moreover, coupling straw and digestate would contribute to decrease the N content in soil surface water. Overall, our results demonstrated that digestate and straw combined application could maintain rice production and have potential positive paddy environmental effects.

scholarly journals Some Peculiarities of Arable Soil Organic Matter Detection Using Optical Remote Sensing Data

2021 ◽  
Vol 13 (12) ◽  
pp. 2313
Author(s):  
Elena Prudnikova ◽  
Igor Savin
Keyword(s):  
Remote Sensing ◽  
Organic Matter ◽  
Soil Organic Matter ◽  
Spectral Reflectance ◽  
Remote Sensing Data ◽  
Soil Surface ◽  
Optical Remote Sensing ◽  
Test Field ◽  
Sensing Data ◽  
Sentinel 2

Optical remote sensing only provides information about the very thin surface layer of soil. Rainfall splash alters soil surface properties and its spectral reflectance. We analyzed the impact of rainfall on the success of soil organic matter (SOM) content (% by mass) detection and mapping based on optical remote sensing data. The subject of the study was the arable soils of a test field located in the Tula region (Russia), their spectral reflectance, and Sentinel-2 data. Our research demonstrated that rainfall negatively affects the accuracy of SOM predictions based on Sentinel-2 data. Depending on the average precipitation per day, the R2cv of models varied from 0.67 to 0.72, RMSEcv from 0.64 to 1.1% and RPIQ from 1.4 to 2.3. The incorporation of information on the soil surface state in the model resulted in an increase in accuracy of SOM content detection based on Sentinel-2 data: the R2cv of the models increased up to 0.78 to 0.84, the RMSEcv decreased to 0.61 to 0.71%, and the RPIQ increased to 2.1 to 2.4. Further studies are necessary to identify how the SOM content and composition of the soil surface change under the influence of rainfall for other soils, and to determine the relationships between rainfall-induced SOM changes and soil surface spectral reflectance.

Accumulation patterns of soil organic matter in forests as a legacy of historical charcoal burning

2021 ◽  
Author(s):  
Anna Schneider ◽  
Alexander Bonhage ◽  
Florian Hirsch ◽  
Alexandra Raab ◽  
Thomas Raab
Keyword(s):  
Land Use ◽  
Organic Matter ◽  
Soil Organic Matter ◽  
Large Scale ◽  
Soil Surface ◽  
Landscape Scale ◽  
Legacy Effects ◽  
Charcoal Production ◽  
Study Region ◽  
Land Use Legacies

<p>Human land use and occupation often lead to a high heterogeneity of soil stratigraphy and properties in landscapes within small, clearly delimited areas. Legacy effects of past land use also are also abundant in recent forest areas. Although such land use legacies can occur on considerable fractions of the soil surface, they are hardly considered in soil mapping and inventories. The heterogenous spatial distribution of land use legacy soils challenges the quantification of their impacts on the landscape scale. Relict charcoal hearths (RCH) are a widespread example for the long-lasting effect of historical land use on soil landscapes in forests of many European countries and also northeastern USA. Soils on RCH clearly differ from surrounding forest soils in their stratigraphy and properties, and are most prominently characterized by a technogenic substrate layer with high contents of charcoal. The properties of RCH soils have recently been studied for several regions, but their relevance on the landscape scale has hardly been quantified.</p><p>We analyse and discuss the distribution and ecological relevance of land use legacy soils across scales for RCH in the state of Brandenburg, Germany, with a focus on soil organic matter (SOM) stocks. Our analysis is based on a large-scale mapping of RCH from digital elevation models (DEM), combined with modelled SOM stocks in RCH soils. The distribution of RCH soils in the study region shows heterogeneity at different scales. The large-scale variation is related to the concentration of charcoal production to specific forest areas and the small-scale accumulation pattern is related to the irregular distribution of single RCH within the charcoal production fields. Considerable fractions of the surface area are covered by RCH soils in the major charcoal production areas within the study region. The results also show that RCH can significantly contribute to the soil organic matter stocks of forests, even for areas where they cover only a small fraction of the soil surface. The study highlights that considering land use legacy effects can be relevant for the results of soil mapping and inventories; and that prospecting and mapping land use legacies from DEM can contribute to improving such approaches.</p>

Biological Control of Sclerotinia Sclerotiorum and Botrytis Spp.

1992 ◽  
pp. 267-271 ◽  
Author(s):  
Nyckle J. Fokkema ◽  
Matthijs Gerlagh ◽  
Jürgen Köhl
Keyword(s):  
Biological Control ◽  
Sclerotinia Sclerotiorum
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