Selenium concentrations in tissues of sheep given a subcutaneous injection of barium selenate or sodium selenate

1994 ◽  
Vol 34 (5) ◽  
pp. 581 ◽  
Author(s):  
JA Archer ◽  
GJ Judson

Young sheep were allocated to 6 treatment groups, each of 8 ewes and 8 wethers. Treatments given were nil, 0.1, or 0.5 mg selenium (Se)/kg body weight as a subcutaneous injection of sodium selenate (Na2SeO4), and 0.8, 1.4, or 2.9 mg Se/kg body weight as a subcutaneous injection of barium selenate (BaSeO4). At 14, 28, 56, and 112 days after treatment, 2 ewes and 2 wethers from each group were slaughtered and samples of blood, liver, kidney, cardiac and skeletal muscle, lymph nodes, and faeces were collected for Se assay. On days 0, 1, 3, 7, 13, 27, and 55 blood and spot faecal samples were taken for Se assay from the 24 sheep selected for slaughter on day 112. In sheep given Na2Se04 there was a rapid but transient increase in the mean Se concentration in tissues, particularly the liver of sheep given the highest dose of Na2SeO4. In these sheep on days 14,28,56, and 112 the respective mean liver concentrations were 169, 62,25, and 6.9 �mol/kg DM: the mean value on day 14 was 15 times the mean value in untreated sheep. In sheep given BaSeO4 there was a gradual increase in mean Se concentration of tissues during the experiment. Faecal Se concentrations increased in sheep given the Se injections. The marked but transient increase in liver Se concentrations in sheep given Na2SeO4 suggests that this organ provides an important protective mechanism against toxicity by readily accumulating and excreting Se into the gut. Our results indicate that the BaSeO4 doses were unlikely to cause Se toxicity in young sheep or result in Se residues in tissues above those recommended for human consumption. The BaSeO4 should be administered subcutaneously in the neck or other sites of the body not sold for human consumption, since deposits of BaSeO4 remain at the site of injection for at least 112 days and may be dangerous if inadvertently consumed.

1959 ◽  
Vol 14 (2) ◽  
pp. 174-176 ◽  
Author(s):  
J. F. Nunn ◽  
E. J. M. Campbell ◽  
B. W. Peckett

The mean volume of the extrathoracic respiratory tract in six cadavers was found to be 72 ml (BTPS), (S.D. ±32). Expressed as a fraction of body weight in pounds this amounted to 0.55. The intrathoracic anatomical dead space was measured in three intubated subjects. The mean value was 66 ml (BPTS), (S.D. ±29) or 0.43 times the body weight in pounds. The influence of the position of the jaw on the dead space was studied in the six cadavers and three conscious subjects. Depression of the jaw with flexion of the neck produced a mean decrease in the dead space of 31.4 ml while a protrusion of the jaw with extension of the neck increased the dead space by 39.7 ml. Submitted on July 28, 1958


Author(s):  
C. Cheng

1. The number of species found in this investigation is six, of which four are marine and two brackish water.2. The mean value of the body weight and the fecundity varies with the species. The size of a species bears no relation whatever to the reproductive capacity.3. The fecundity is subject to seasonal variation, being generally higher in the spring than in the winter months.4. In general, a positive correlation exists between body weight and fecundity of the individuals of each species. This rule does not, however, apply to Gammarus duebeni, in which the fecundity appears to fall after reaching its maximum at the 19 mg. body-weight class.


1961 ◽  
Vol 3 (03) ◽  
pp. 225-231 ◽  
Author(s):  
Elizabeth A. Walker ◽  
J. H. Taylor

1. Four fitter-mates from 20 litters on each of 3 farms were divided equally and distributed at random between two treatment groups. Each piglet was injected intramuscularly with 2 c.c. of an iron preparation between 1 and 2 days of age. The control group received an iron-dextran complex which provided 150 mg. of iron per piglet and a second group received an irondextrin complex which provided 200 mg. of iron per piglet.2. The body weight and the level of haemoglobin in the blood of each piglet were measured at 1, 8, 15, 22, and 56 days of age and the incidence and cause of disease and mortality were recorded.3. The level of haemoglobin in the blood of both groups was comparable throughout the experiment.4. At the 22nd and 56th day, the group receiving 200 mg. of iron was approximately 6% heavier than the group receiving 150 mg. of iron. These differences were highly significant at the 0·1% level.5. From each of 3 additional litters, 3 piglets received no supplementary iron while 5 piglets received 200 mg. of iron provided by the iron-dextrin complex. The three whole litters were slaughtered on days 3, 8, and 22 respectively. The mean level of iron per 100 g. of liver and spleen within the untreated group on the above occasions was 5·8, 5·8, and 3·7 mg. respectively, compared with 72·9, 55·1, and 17·6 mg. respectively within the treated piglets. The differences were highly significant at 0·1 % level.


2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.


1984 ◽  
Vol 98 (12) ◽  
pp. 1213-1216 ◽  
Author(s):  
Harbans Lal ◽  
O. P. Sachdeva ◽  
H. R. Mehta

AbstractSerum immunoglobulin (IgG, IgA and IgM) levels were determined in patients with chronic tonsillitis before and one month after tonsillectomy. The preoperative levels of serum IgG, IgA and IgM were significantly higher when compared with the controls. The increase may be due to repeated antigenic stimulation. The post-operative levels for the three immunoglobulins were decreased; however, a significant reduction was observed for IgG only where the mean value was comparable with the control group. The data confirm that tonsillectomy does not disturb the humoral immune system of the body.


2005 ◽  
Vol 129 (1) ◽  
pp. 89-91 ◽  
Author(s):  
Mordechai Lorberboym ◽  
Naomi Rahimi-Levene ◽  
Helena Lipszyc ◽  
Chun K. Kim

Abstract Context.—Polycythemia describes an increased proportion of red blood cells in the peripheral blood. In absolute polycythemia, there is increased red cell mass (RCM) with normal plasma volume, in contrast with apparent polycythemia, in which there is increased or normal RCM and decreased plasma volume. In order to deliver the appropriate treatment it is necessary to differentiate between the two. Objective.—A retrospective analysis of RCM and plasma volume data are presented, with special attention to different methods of RCM interpretation. Design.—The measurements of RCM and plasma volume in 64 patients were compared with the venous and whole-body packed cell volume, and the incidence of absolute and apparent polycythemia was determined for increasing hematocrit levels. Measurements of RCM and plasma volume were performed using chromium 51–labeled red cells and iodine 125–labeled albumin, respectively. The measured RCM of each patient was expressed as a percentage of the mean expected RCM and was also defined as being within or outside the range of 2 SD of the mean. The results were also expressed in the traditional manner of mL/kg body weight. Results.—Twenty-one patients (13 women and 8 men) had absolute polycythemia. None of them had an increased plasma volume beyond 2 SD of the mean. When expressed according to the criteria of mL/kg body weight, 17 of the 21 patients had abnormally increased RCM, but 4 patients (19%) had a normal RCM value. Twenty-eight patients had apparent polycythemia. The remaining 15 patients had normal RCM and plasma volume. Conclusions.—The measurement of RCM and plasma volume is a simple and necessary procedure in the evaluation of polycythemia. In obese patients, the expression of RCM in mL/kg body weight lacks precision, considering that adipose tissue is hypovascular. The results of RCM are best described as being within or beyond 2 SD of the mean value.


1971 ◽  
Vol 55 (3) ◽  
pp. 651-665 ◽  
Author(s):  
G. M. HUGHES ◽  
R. GAYMER ◽  
MARGARET MOORE ◽  
A. J. WOAKES

1. The O2 consumption and CO2 release of nine giant tortoises Testudo gigantea (weight range 118 g-35·5 kg) were measured at a temperature of about 25·5°C. Four European tortoises Testudo hermanni (weight range 640 g-2·16 kg) were also used. The mean RQ values obtained were 1·01 for T. gigantea and 0·97 for T. hermanni. These values were not influenced by activity or size. 2. The data was analysed by plotting log/log regression lines relating body weight to O2 consumption. Both maximum and minimum metabolic rates recorded for each individual T. gigantea showed a negative correlation with body weight. For active rates the relation was O2 consumption = 140·8W0·97, whereas for inactive animals O2 consumption = 45·47W0·82. 3. The maximum rates were obtained from animals that were observed to be active in the respirometer and the minimum rates from animals that remained quiet throughout. The scope for activity increased with body size, being 82 ml/kg/h for animals of 100 g and 103 ml/kg/h for 100 kg animals. The corresponding ratio between maximum and minimum rates increases from about 2 to 6 for the same weight range. 4. Values for metabolic rate in T. hermanni seem to be rather lower than in T. gigantea. Analysis of the relative proportion of the shell and other organs indicates that the shell forms about 31% of the body weight in adult T. hermanni but only about 18% in T. gigantea of similar size. The shell is not appreciably heavier in adult T. gigantea (about 20%). 5. Data obtained for inactive animals is in good agreement with results of other workers using lizards and snakes. Previous evidence suggesting that chelonians show no reduction in metabolic rate with increasing size is not considered to conflict with data obtained in the present work.


2002 ◽  
Vol 50 (3) ◽  
pp. 365-371 ◽  
Author(s):  
L. Várnagy ◽  
P. Budai ◽  
E. Molnár ◽  

The reproductive toxicity of lead acetate and of a fungicide formulation (Dithane M-45) containing 80% mancozeb was studied on rats. Lead acetate was applied in the feed in the following dose groups: control, 1,000, 5,000 and 10,000 mg/kg of diet. The three treatment groups received, in addition to the above doses of lead acetate, 4,500 mg/kg Dithane M-45 in the diet. The method was based on the OECD Guideline for Testing of Chemicals No. 415 (1981). Clinical symptoms and mortality were not found in the parent generation. The body weight of female animals decreased significantly before the pregnancy period. This tendency was also seen in males after the combination treatment. At the two high dose levels a remarkable body weight increase was seen in the female animals during the lactation period. As a result of treatment, decreased body weight of offspring was measured during the lactation period. No gross pathological changes were seen. Histological examination showed general tubulonephrosis in the experimental animals. It can be established that the administration of Dithane M-45 did not enhance the reproductive toxicity of lead acetate.


2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Filippo Giarratana ◽  
Graziella Ziino ◽  
Valerio D'Andrea ◽  
Antonio Panebianco ◽  
Alessandro Giuffrida

n the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter’s fish (Zeus faber). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of Zeus faber ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations. Fresh and frozen samples have significant (P<0.01) differences in total bacterial charge, with values of 4.75±0.5 Log CFU/g and 3.65±0.7 Log CFU/g respectively. The mean value of Enterobacteriaceae was 2.58±0.7 Log CFU/g in fresh products, while 52.17% (12) of frozen samples reported loads of <1 Log CFU/g. No Salmonella spp. and Listeria monocytogenes were found. Aeromonas spp. was detected in two frozen sample (with loads of 2.2 and <1 Log CFU/g) and in 5 fresh ovaries with value ranged from 1.70 to 3.48 Log CFU/g. Vibrio spp. was found in 4 (36.36%) and 3 (13.04%) of fresh and frozen samples respectively, with loads always <1 Log CFU/g. All 31 Vibrio strains isolated, were identified as Vibrio alginolyticus, and 61.29% (19) of them was positive for the ToxRS factor and 6.45% (2) for ToxR. The 47.06% (16) of total samples showed infestations by larvae of Anisakis Type 1 in the serous and inside the ovary. In this last case, histologically it was found to be free larvae. This study attested satisfactory hygiene conditions for Zeus faber ovaries currently marked for human consumption. The presence of potentially pathogenic strains of V. alginolyticus and Aeromonas spp., but above all the frequent infestation by Anisakis larvae, represent a potentially hazard for the consumer.


2016 ◽  
Vol 7 (2) ◽  
pp. 26
Author(s):  
Wanmi Nathaniel ◽  
Onyeanusi I. Barth ◽  
Nzalak J. Oliver ◽  
Aluwong Tanang

<p class="jbls-body"><span lang="EN-GB">A total of one hundred and seventy-three fertilized eggs were used for morphometry, gross and histological studies. At day 4 of incubation, the mean body weight of the helmeted guinea fowl embryo was 0.6401 ± 0.0211 g. It was at day 10 of incubation that there was an increase in the whole body weight of the embryo to be 0.8650 ± 0.676 g. The whole brain weight indicated relative increased at day 4 as compared to that of the whole body weight. Graphically, there were steady increase in the body, brain and optic lobe weights. Histologically, cells and neurones that make up the optic lobe is probably as a result of the migration of immature cells from the ventricular neuroepithelium. </span></p>


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