Isolation of dehydration-responsive genes in a drought tolerant common bean cultivar and expression of a group 3 late embryogenesis abundant mRNA in tolerant and susceptible bean cultivars

2007 ◽  
Vol 34 (4) ◽  
pp. 368 ◽  
Author(s):  
Blanca E. Barrera-Figueroa ◽  
Julián M. Peña-Castro ◽  
Jorge A. Acosta-Gallegos ◽  
Roberto Ruiz-Medrano ◽  
Beatriz Xoconostle-Cázares
Keyword(s):  
Common Bean ◽  
Expression Profiles ◽  
Sugar Metabolism ◽  
Complete Sequence ◽  
Late Embryogenesis Abundant ◽  
Suppression Subtractive Hybridisation ◽  
Cellular Protection ◽  
Drought Tolerant ◽  
Late Embryogenesis ◽  
Group 3

Drought is one of the main constraints for common bean (Phaseolus vulgaris L.) production in Latin America. The aim of this work was to identify upregulated genes in the drought-tolerant common bean cv. Pinto Villa, grown under water-deficit conditions. Twenty-eight cDNAs representing differentially-expressed mRNAs in roots and/or leaves were isolated via suppression subtractive hybridisation. Their expression profiles in plants under intermediate and severe dehydration stress were tested. Three cDNAs corresponded to genes already described as associated to drought stress in P. vulgaris, 12 were known P. vulgaris sequences without previous association with drought response, and 13 were new P. vulgaris sequences. Analysis of the deduced proteins encoded by the cDNAs revealed putative functions in cellular protection, sugar metabolism, and protein synthesis, folding and turnover. Additionally, a new member of group 3 late embryogenesis abundant (LEA) genes (PvLEA3) was cloned and its complete sequence was obtained. Given the lack of reports comparing expression of dehydration-responsive genes in bean cultivars with different response to drought, the expression of PvLEA3 transcript in five bean cultivars from different origin was analysed. The induction of PvLEA3 was directly associated with the level of drought tolerance in the cultivars studied.

scholarly journals Genetic Transformation of Common Bean (Phaseolus vulgarisL.) with theGusColor Marker, theBarHerbicide Resistance, and the Barley (Hordeum vulgare)HVA1Drought Tolerance Genes

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Kingdom Kwapata ◽  
Thang Nguyen ◽  
Mariam Sticklen
Keyword(s):  
Hordeum Vulgare ◽  
Common Bean ◽  
Late Embryogenesis Abundant ◽  
Shoot Meristem ◽  
Late Embryogenesis Abundant Protein ◽  
Herbicide Resistant ◽  
Late Embryogenesis ◽  
Color Marker ◽  
Selection Of

Five common bean (Phaseolus vulgarisL.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes includedguscolor marker which visually confirmed transgenic events, thebarherbicide resistance selectable marker used forin vitroselection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of betterP. vulgarisgermplasm.

scholarly journals Drought Tolerance in Wheat

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Arash Nezhadahmadi ◽  
Zakaria Hossain Prodhan ◽  
Golam Faruq
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Review Paper ◽  
Expression Patterns ◽  
Late Embryogenesis Abundant ◽  
Glutathione S Transferase ◽  
Molecular Responses ◽  
Drought Tolerant ◽  
Late Embryogenesis ◽  
Different Types

Drought is one of the most important phenomena which limit crops’ production and yield. Crops demonstrate various morphological, physiological, biochemical, and molecular responses to tackle drought stress. Plants’ vegetative and reproductive stages are intensively influenced by drought stress. Drought tolerance is a complicated trait which is controlled by polygenes and their expressions are influenced by various environmental elements. This means that breeding for this trait is so difficult and new molecular methods such as molecular markers, quantitative trait loci (QTL) mapping strategies, and expression patterns of genes should be applied to produce drought tolerant genotypes. In wheat, there are several genes which are responsible for drought stress tolerance and produce different types of enzymes and proteins for instance, late embryogenesis abundant (lea), responsive to abscisic acid (Rab), rubisco, helicase, proline, glutathione-S-transferase (GST), and carbohydrates during drought stress. This review paper has concentrated on the study of water limitation and its effects on morphological, physiological, biochemical, and molecular responses of wheat with the possible losses caused by drought stress.

scholarly journals Proteomics of Bulked Rachides Combined with Documented QTL Uncovers Genotype Nonspecific Players of the Fusarium Head Blight Responses in Wheat

2019 ◽  
Vol 109 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Jiajun Liu ◽  
Lei Li ◽  
Nora A. Foroud ◽  
Xuan Gong ◽  
Changcheng Li ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
High Throughput ◽  
Late Embryogenesis Abundant ◽  
Purple Acid Phosphatase ◽  
Label Free ◽  
Head Blight ◽  
Fusarium Spp ◽  
Late Embryogenesis ◽  
Wide Range ◽  
Group 3

Fusarium head blight (FHB) is a destructive disease of wheat that reduces yield and grain quality. High-throughput proteomic techniques have been used to identify a wide range of candidate proteins involved in host resistance. The majority of the published works on the proteomics of the wheat response to Fusarium graminearum infection are case specific. In the current study, a high-throughput quantitative label-free strategy was employed on bulked rachides of F. graminearum-infected wheat collected from multiple genotypes. Differentially accumulated proteins among the following four pools were identified: mock-inoculated FHB-resistant accessions (RM), mock-inoculated FHB-susceptible accessions (SM), F. graminearum-inoculated FHB-resistant accessions (RFg), and F. graminearum-inoculated FHB-susceptible accessions (SFg). Four pairs of comparisons were made: RFg versus RM, SFg versus SM, RM versus SM, and RFg versus SFg. Proteins were projected onto the consensus intervals of previously reported quantitative trait loci in the FHB-resistant pool by blasting against the Chinese Spring reference sequences. In addition to proteins previously reported in the host response to Fusarium spp., new candidates have emerged in association with resistance or susceptibility, including a group 3 late embryogenesis abundant as a resistance-related protein and a purple acid phosphatase as a susceptibility protein. The protein atlas presented here provides new perspectives on the interaction between F. graminearum and wheat.

scholarly journals Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 696
Author(s):  
Datong Liu ◽  
Jing Sun ◽  
Dongmei Zhu ◽  
Guofeng Lyu ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Expression Profiles ◽  
Triticum Aestivum L ◽  
Late Embryogenesis Abundant ◽  
Wheat Breeding ◽  
Pcr Analysis ◽  
Lea Genes ◽  
Cellular Dehydration ◽  
Genome Wide ◽  
Late Embryogenesis

Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we carried out a series of analyses based on the latest genome sequence of the bread wheat Chinese Spring. 121 Triticum aestivum L. LEA (TaLEA) genes, classified as 8 groups, were identified and characterized. TaLEA genes are distributed in all chromosomes, most of them with a low number of introns (≤3). Expression profiles showed that most TaLEA genes expressed specifically in grains. By qRT-PCR analysis, we confirmed that 12 genes among them showed high expression levels during late stage grain maturation in two spring wheat cultivars, Yangmai16 and Yangmai15. For most genes, the peak of expression appeared earlier in Yangmai16. Statistical analysis indicated that expression level of 8 genes in Yangmai 16 were significantly higher than Yangmai 15 at 25 days after anthesis. Taken together, our results provide more knowledge for future functional analysis and potential utilization of TaLEA genes in wheat breeding.

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