The Catabolism of Nitrogen Compounds in Dendrostomun cymodoceae Edmonds (Sipunculoidea)

1957 ◽  
Vol 8 (2) ◽  
pp. 131 ◽  
Author(s):  
SJ Edmonds
Keyword(s):  
Uric Acid ◽  
Amino Acid ◽  
Total Nitrogen ◽  
Ammonia Nitrogen ◽  
Nitrogen Compounds ◽  
Protein Nitrogen ◽  
Urea Nitrogen ◽  
Amino Acid Nitrogen ◽  
Sipunculus Nudus

The total non-protein nitrogen in the excretory products of fasting specimens of Dendrostomum cymodoceae Edmonds was found to be 1.18-1.40 mg N/100 g wet wt./24 hr. Of the total nitrogen, 83-90 per cent. was excreted as ammonia nitrogen, 4-6 percent, as urea nitrogen, 0-4 percent. as amino acid nitrogen, and none as uric acid; 89-94 percent. of the nitrogen was thus accounted for. The results are compared with the corresponding values obtained by other workers for Sipunculus nudus and some other invertebrates.

scholarly journals CHEMICAL CHANGES IN THE BLOOD OF THE DOG AFTER INTESTINAL OBSTRUCTION

1923 ◽  
Vol 37 (3) ◽  
pp. 365-375 ◽  
Author(s):  
Russell L. Haden ◽  
Thomas G. Orr
Keyword(s):  
Uric Acid ◽  
Amino Acid ◽  
Intestinal Obstruction ◽  
Ileocecal Valve ◽  
The Body ◽  
Protective Measure ◽  
Protein Nitrogen ◽  
Urea Nitrogen ◽  
Amino Acid Nitrogen ◽  
Chlorine Ion

A study of the non-protein nitrogen, urea nitrogen, uric acid, creatinine, amino-acid nitrogen, sugar, and chlorides of the blood and the CO2-combining power of the plasma in normal dogs, and in dogs after different types of intestinal obstruction, is reported. Following ligation of the duodenum, ligation of the duodenum with gastroenterostomy, and ligation of the upper half of the ileum, a fall in chlorides and a rise in the non-protein nitrogen and urea nitrogen of the blood and in the CO2-combining power of the plasma occur. The uric acid, creatinine, amino-acid nitrogen, and sugar show no significant changes. The fundamental change is a fall in chlorides followed by an alkalosis. The degree of alkalosis depends upon the rate of formation of carbonate, rate of excretion by the kidneys, and extent of neutralization of the carbonate by acid bodies formed during the intoxication. The fall in chlorides is probably due to a utilization of the chlorine ion in the course of the intoxication. It is suggested that this use of chlorine is a protective measure on the part of the body. There are indications that high intestinal obstruction should not be treated by the administration of alkalies. The urea nitrogen is a good index of the protein destruction. Ligation of the ileum at the ileocecal valve is followed by little increase in nitrogen and no change in the chlorides or CO2-combining power of the plasma. The close similarity of the blood findings in intestinal obstruction, acute lobar pneumonia, and serum disease suggests that these widely different conditions may have a common chemical basis.

scholarly journals Urinary nitrogen compounds in dairy cows fed urea as the sole or partial source of nitrogen

1976 ◽  
Vol 48 (4) ◽  
pp. 323-335
Author(s):  
Terttu Ettala ◽  
Matti Kreula
Keyword(s):  
Uric Acid ◽  
Dairy Cows ◽  
Total Nitrogen ◽  
Ammonium Nitrogen ◽  
Nitrogen Compounds ◽  
Urea Nitrogen ◽  
Urinary Nitrogen

Urinary nitrogen compounds were determined in test cows with urea as the sole (0-cows) or partial (ULP-cows) source of nitrogen. An average of 0.49 % total nitrogen was found in 0-cows and 0.88 % in ULP-cows, the values for urea nitrogen being 2.24 and 2.63 mg/ml, for ammonium nitrogen 0.14 and 0.09 mg/ml, for creatinine 0.77 and 0.90 mg/ml and for creatine 0.28 and 0,42 mg/ml urine, respectively. Differences between the two groups were highly significant (P < 0.001) as regards total nitrogen and significant (P < 0.05) as regards urea nitrogen and creatine. In each group the between-cow differences were highly significant with regard to total and urea nitrogen and creatine, and in 0-cows also with regard to ammonium nitrogen and creatinine. Smaller amounts of urinary allantoin and larger amounts of uric acid were found in 0-cows than in ULP-cows. Corresponding determinations were made to some extent also on the urine of cows on normal indoor or pasture feeding.

DISTRIBUTION OF NITROGEN IN REPRESENTATIVE CANADIAN SOILS

1977 ◽  
Vol 57 (4) ◽  
pp. 445-456 ◽  
Author(s):  
F. J. SOWDEN
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Total Nitrogen ◽  
Amino Sugar ◽  
Peat Soils ◽  
Protein Nitrogen ◽  
Amino Acid Nitrogen ◽  
Canadian Soils ◽  
Mineral Soils

Forms of nitrogen in 92 samples were measured in a study designed to determine the similarities and differences in the nitrogen distribution in various horizons of a wide range of Canadian soils. The samples, representing all of the nine soil orders, were chosen from different climate and vegetation zones. Some peat soils were analyzed also. The data were first grouped according to the nitrogen content of the samples, but the amino acid composition of the soil "protein" did not appear to be related to this. The data were then grouped according to LFH, A, B and C horizons and also according to the Ah, Ap, Ae, Bhf, Bh, Bm and Bt layers. Again, few significant differences in the amino acid composition could be found. Data for the average amino acid composition and standard deviations for 92 mineral soils, 6 LFH and 2 ’O’ horizons of these and 18 peat soils were calculated. Since the analytical and sampling errors appear to be relatively small and would not account for all the variation between samples, there appeared to be real but relatively small and random differences in the amino acid composition of the different samples. The data for the individual soils supported this conclusion; for instance, some samples had very small or barely detectable amounts of hydroxyproline, while with other soils it made up 1–2% of the amino acid nitrogen. The amino sugar composition was more variable and the glucosamine/galactosamine ratio varied from 2:1 for the LFH horizons to 1:1 for the peat soils. In general, however, the soil "protein", which is probably largely the result of microbial degradation and synthesis, is remarkably similar to its amino acid composition. Amino acid nitrogen made up over half of the total nitrogen of the LFH and O horizons. This underestimated the "protein" nitrogen, since there is probably some amide nitrogen (about 5%) not included. In the mineral soils probably about 40% was "protein" nitrogen (including aminde), 5% was amino sugar nitrogen, 18% hydrolyzable unidentified nitrogen and 13.5% was insoluble in the acid used for hydrolysis. Clay-fixed ammonium made up 17% of the total nitrogen and much of the hydrolyzable ammonium came from this.

BLOOD NORMALS FOR CATTLE; SOME PATHOLOGICAL VALUES

1930 ◽  
Vol 3 (2) ◽  
pp. 120-124 ◽  
Author(s):  
John Allardyce ◽  
R. H. Fleming ◽  
F. L. Fowler ◽  
R. H. Clark
Keyword(s):  
Amino Acid ◽  
Cholesterol Content ◽  
Inorganic Phosphorus ◽  
Normal Range ◽  
Protein Nitrogen ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Amino Acid Nitrogen ◽  
Cattle Blood

Samples of cattle blood have been analysed and the concentrations of 10 constituents determined, i.e., cholesterol, sugar, non-protein nitrogen, urea nitrogen, amino-acid nitrogen, creatine, creatinine, calcium, inorganic phosphorus and chlorides. The normal range of each of these constituents was established. The diet fed to the cattle influences the cholesterol content markedly. Blood samples of cattle suffering from red water and some other pathological cases have been examined, and in each case the results show the variation of the contents of the various constituents.

The non-protein nitrogen composition of grass silage:III. The composition of spoilt silages

1971 ◽  
Vol 76 (3) ◽  
pp. 329-336 ◽  
Author(s):  
A. D. Hughes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nitrogen Content ◽  
Ammonia Nitrogen ◽  
Water Soluble ◽  
Protein Nitrogen ◽  
High Ph ◽  
Selective Degradation ◽  
Amino Acid Nitrogen ◽  
Three Samples

SUMMARYThe nitrogenous composition of the water soluble, non-protein nitrogen fractions of three samples of high-pH-spoilt silage and one sample of overheated silage were examined in detail. The amino acid nitrogen content of the high-pH-spoilt silages declined with increasing extent of spoilage, due to a selective degradation of the amino acids. Losses of amino acids were associated with proportionate increases in the amount of volatile basic nitrogen. Lower aliphatic amines were not present and it was therefore concluded that the ultimate end product of nitrogen metabolism in high-pH-spoilt silages was ammonia. Only low concentrations of putrefaction products, such as putroscine, cadaverine and histamine were present.The composition of the non-protein nitrogen fraction of the overheated silage was unusual in that the ammonia nitrogen content was high and associated with the complete destruction of certain amino acids. The free amino acid nitrogen content was low but this was partially compensated for by a very high ‘peptide’ nitrogen content. There was no evidence of putrefaction products in this silage.

scholarly journals Studies on the nitrogen compounds of the faeces of dairy cows fed urea as the sole or partial source of nitrogen

1979 ◽  
Vol 51 (1) ◽  
pp. 506-513
Author(s):  
Terttu Ettala ◽  
Matti Kreula
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dairy Cows ◽  
Acid Hydrolysis ◽  
Free Amino Acid ◽  
Total Nitrogen ◽  
Free Amino ◽  
Diaminopimelic Acid ◽  
Nitrogen Compounds ◽  
Soluble Nitrogen

The nitrogen compounds of the faeces of dairy cows on purified, protein-free feed with urea and small amounts of ammonium salts as the sole source of nitrogen (0-feed). on low-protein feed in which part of the protein was replaced with urea (ULP-feed) and on normal protein-containing feed (NorP-feed) were studied. The total nitrogen contents of the dry matter of the faeces were 2.5 ± 0.7 % on 0-feed, 2.4 ± 0.4 % on ULP-feed and 2.3 ± 0.6 % on NorP-feed. The proportion of urea- and ammonium nitrogen of the total nitrogen was 3.2 ± 2.4, 4.5 ± 2.4 and 3.7 ± 1.8 % respectively, and the proportion of nitrogen soluble in 70 % ethanol 10.9 ± 5.7, 9.9 ± 2.4 and 10.4 ± 3.8 % of the total nitrogen; the proportion of nitrogen in the free amino acid fraction was 4.1 ±2.9 on 0-feed, 1.6 ± 1.4 (P < 0.05) on ULP-feed and 3.2 ± 2.9 % on NorP-feed. The nitrogen insoluble in 70 % ethanol as a proportion of the total nitrogen was 82.3 ± 7.2 on 0-feed, 85.4 ± 5.0 on ULP-feed and 82.3 ± 7.2 % on NorP-feed. Of the total nitrogen of this insoluble fraction, the following proportions were released as amino acids by acid hydrolysis: 62.15 ± 8.5 % on 0-feed, 61.6 ± 10.7 % on ULP-feed and 59.0 ± 13.9% on NorP-feed. (α, Ɛ-diaminopimelic acid was 1.7 ± 0.6, 0.7 ± 0.2 (P < 0.05) and 1.2 ± 0,4 mol % respectively of the total amino acids of the hydrolyzate. The soluble nitrogen fraction was separated into free amino acid and »peptide» fractions, and the amino acid composition of both was determined, the latter after acid hydrolysis

The Energy-Sources in Ontogenesis

1926 ◽  
Vol 4 (2) ◽  
pp. 114-144
Author(s):  
JOSEPH NEEDHAM
Keyword(s):  
Uric Acid ◽  
Total Nitrogen ◽  
Total Protein ◽  
Acid Content ◽  
Acid Production ◽  
Maximum Intensity ◽  
Energy Sources ◽  
Chemical Analyses ◽  
Protein Nitrogen ◽  
Hen’S Egg

1. The uric acid content of the hen's egg has been investigated from the fourth to the twentieth day of incubation. There is a period of intensive uric acid production from the seventh to the eleventh day. After that point the excretion of uric acid fails to keep pace with the growth and differentiation of the embryo. 2. The point of maximum intensity of uric acid production occurs two days later than the point of maximum intensity in the production of urea. 3. From the fourth to the seventh day more urea is present than uric acid, and more is excreted, but by the tenth day the adult relationship is attained, in which 95 per cent. of the total nitrogen excreted is uric acid. 4. The maximum intensity of protein combustion is attained between the eighth and the ninth days. It is pointed out that this occurs midway between the periods when carbohydrate and fat are respectively the predominant energy-sources. 5. The protein used as a source of energy belongs entirely to the coagulable fraction; ovomucoid is not employed for this purpose. 6. The protein nitrogen lost by combustion during development amounts to 7.5 per cent. of the total protein nitrogen present at the beginning, and to 3.0 per cent. of the total foodstuff burnt. 7. The R.Q. for each day of incubation has been calculated on the basis of chemical analyses of fat, protein, and carbohydrate, and agrees as well as can be expected at present with those experimentally determined by Bohr and Hasselbalch, and by Lussanna. 8. Further evidence has been collected from the literature indicating that in embryogenesis there is a succession of sources of energy, carbohydrate preceding protein, and protein preceding fat. 9. Injection experiments and other considerations lead to the conclusion that factors located in the embryo decide what the embryo shall make use of as a source of energy. It does not, for instance, combust protein because its supply of available carbohydrate has been exhausted.

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