209 EXPRESSION OF GENES RELATED TO OVULATORY CAPACITY (LHR AND AGTR2) IN GRANULOSA CELLS FROM SUPERSTIMULATED OR NOT SUPERSTIMULATED ANGUS COWS

2014 ◽  
Vol 26 (1) ◽  
pp. 218 ◽  
Author(s):  
E. Lucacin ◽  
A. R. Pupulim ◽  
P. K. Fontes ◽  
E. M. Razza ◽  
M. F. Machado ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Target Genes ◽  
Animal Health ◽  
Treatment Protocol ◽  
Sao Paulo ◽  
Mrna Abundance ◽  
Control Group ◽  
Mrna Levels ◽  
São Paulo ◽  
Intravaginal Device

Multiple ovulation and embryo transfer (MOET) has contributed to genetic improvement of Brazilian cattle and the P36 superstimulatory treatment protocol has been used successfully in MOET programs. Recently, increased mRNA levels of LH receptor (LHR) and angiotensin II receptor type 2 (AGTR2) have been reported in granulosa cells (GC) of superstimulated Nelore cows using P36 protocol and the efficacy of the P36 protocol has been investigated with the addition of a LH stimulus (eCG or LH), on the last day of treatment. We aimed to evaluate mRNA abundance of AGTR2 and LHR, related to ovulatory capacity, in GC of superstimulated Angus cows by different variations of the P36 protocol. Multiparous Angus cows (n = 37) were randomly assigned to four groups: control, P36, P36+eCG, and P36/FSH+LH. The control group (non-superstimulated cows, n = 7) received an intravaginal device containing progesterone (1.0 g; Primer®; Agener Animal Health, São Paulo, SP, Brazil) and 2.5 mg of oestradiol benzoate (EB; RIC-BE®; Agener Animal Health; IM) on a random day of the oestrous cycle (Day 0). After 8 days (Day 8) cows were treated with PGF2α (150 mg d-cloprostenol; Prolise®; Agener Animal Health; IM; 7:00 a.m.) and the intravaginal device was removed (7:00 p.m.). Cows from P36 group (n = 10) received the intravaginal device and 2.5 mg of EB at the beginning of the protocol (Day 0). Starting 5 days later (Day 5), 200 mg pFSH (Folltropin®; Bioniche Animal Health, Belleville, ON, Canada) was administered twice daily by IM injections over 4 days; on Day 7, PGF2α was administered and the intravaginal device was removed 36 h later (Day 8, 7:00 p.m.). Groups with the addition of a LH stimulus (eCG or LH administration) were submitted to the P36 protocol with replacement of the last two FSH doses by 200 IU eCG (P36+eCG; Novormon®, MSD Animal Health, São Paulo, SP, Brazil, IM; n = 10) or 2 mg pLH concomitant with the last two doses of FSH (P36/FSH+LH; Lutropin®, Bioniche Animal Health, IM; n = 10). Cows in all groups were slaughtered on Day 9, 12 h after progesterone-device removal and ovaries were transported on ice to the laboratory immediately after slaughter. Non-superstimulated dominant follicles and superstimulated follicles were dissected from the ovaries to obtain GC and total RNA was extracted. Amplification of housekeeping (peptidyl-prolyl isomerase A; PPIA) and target genes was performed by real-time RT–PCR according to the Sybr Green protocol. Relative gene expression was determined by Pfaffl's equation. The mRNA abundance was compared by Tukey's test and the significance level was P < 0.05. In GC, the mRNA abundance (mean ± s.e.m.) of LHR and AGTR2 was higher in the control group (1.02 ± 0.24 and 3.76 ± 1.07, respectively; P < 0.05) when compared with the superstimulated groups: P36 (0.33 ± 0.24 and 1.02 ± 0.21), P36+eCG (0.42 ± 0.10 and 1.40 ± 0.33) and P36/FSH+LH (0.38 ± 0.07 and 1.45 ± 0.49). In conclusion, the superstimulatory treatments decrease mRNA levels of LHR and AGTR2. We acknowledge CAPES for E. Lucacin's scholarship and FAPESP–São Paulo Research Foundation for grant number 2011/50593-2.

25 ADDITION OF FSH, IN CONTRAST TO eCG, DOES NOT INCREASE PREGNANCY RATES IN ANESTROUS NELLORE (BOS INDICUS) COWS TREATED WITH FIXED-TIME AI PROTOCOL

2010 ◽  
Vol 22 (1) ◽  
pp. 170 ◽  
Author(s):  
L. A. Lima ◽  
V. G. Pinheiro ◽  
J. R. Cury ◽  
C. M. Barros
Keyword(s):  
Pregnancy Rate ◽  
Buenos Aires ◽  
Animal Health ◽  
Bos Indicus ◽  
Fixed Time ◽  
Sao Paulo ◽  
Pregnancy Rates ◽  
Control Group ◽  
São Paulo ◽  
Intravaginal Device

There are reports in the literature indicating that use of eCG improves pregnancy rates in Bos indicus anestrous cows treated with a progesterone-based fixed-time AI (FTAI) protocol. More recently, replacement of eCG by FSH in FTAI protocols was reported to have beneficial effects (Bos indicus) or no effect (Bos taurus) on pregnancy rates. In the present experiment the effects of eCG and FSH on pregnancy rates were compared in an FTAI protocol. Primiparous lactating Nellore cows (40 to 80 days postpartum, n = 421) with a body condition score of 2.5 to 3.0 (on a 1 to 5 scale) were randomly allocated in 3 groups: Control (CTR), eCG, and FSH. In the control group, all animals received a progesterone (P4)-releasing intravaginal device (1.55 g, PRID®, Ceva Sante Animale S.A., Libourne, France) and 2.5 mg of estradiol benzoate (EB, i.m. Estrogin®, Farmavet, São Paulo, Brazil), on Day 0 (D0). Eight days later (D8), at the time of the intravaginal device withdrawal, PGF2α (150 μg, D-cloprostenol, i.m. Prolise®, ARSA S.L.R., Buenos Aires, Argentina) was administered. Twenty-four hours after PRID removal, cows were treated with EB (1.0 mg, i.m.), and FTAI was done 30 to 36 h later. In the eCG and FSH groups, the cows were treated with 20 mg of FSH (Folltropin-V®, i.m. Bioniche, Belleville, Canada) or400IU of eCG (Novormon®, i.m. Syntex, Buenos Aires, Argentina), respectively, at the time of PGF2α administration. Ovarian ultrasonography (Aloka SSD 500, 7.5-MHz probe, Aloka, Tokyo, Japan) was performed 10 days prior to and at the beginning of FTAI protocol to select the animals in postpartum anestrous (absence of CL in both examinations). The pregnancy diagnosis was performed by ultrasonography 30 days after FTAI. Data were analyzed by logistic regression (SAS Institute, Cary, NC, USA). Cows treated with eCG had a higher pregnancy rate (41.5%, 95/229; P < 0.05) than those treated with FSH (22.7%, 22/97) or in the control group (26.3%, 25/95). Pregnancy rates of animals treated with FSH did not differ (P > 0.05) from those in the control group. These results indicated that, in lactating primiparous anestrous Nellore cows, the use of eCG in a FTAI protocol improves the pregnancy rate, whereas FSH has no beneficial effect. V. G. Pinheiro received a fellowship from FAPESP (São Paulo, Brazil). The authors are grateful to Ceva Animal Health for providing the intravaginal devices (PRID®) used in this experiment.

13 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIMED ARTIFICIAL INSEMINATION IN NELLORE COWS (BOS TAURUS INDICUS)

2010 ◽  
Vol 22 (1) ◽  
pp. 164
Author(s):  
F. A. Braga ◽  
F. J. D. Pardo ◽  
P. H. Miguez ◽  
A. Kehrle ◽  
J. R. V. Pimentel ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Pregnancy Rates ◽  
Control Group ◽  
São Paulo ◽  
Effect Of Treatment ◽  
Intravaginal Device

Artificial insemination results can be improved using fixed-timed AI (FTAI). Currently, progesterone release devices, indispensable in FTAI procedures, such as DIB® (Schering-Plough, Kenilworth, NJ, USA), are made of silicone, a nonbiodegradable biocompatible polymer. Recently, biodegradable progesterone (P4)-releasing devices made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactona (PCL) have been developed. To investigate the efficiency on pregnancy rate of FTAI programs using a new intravaginal device made of PHB-V (Progestar®, Innovare, São Paulo, Brazil), 240 Nellore (Bos indicus) cows, between 3 and 8 years of age, were randomly assigned to 2 experiments. In experiment 1, new P4 devices DIB (control group, DIB1; n = 60) were compared with PHB-V (Progestar1; n = 76); in experiment 2, previously used DIB (DIB2; n = 52) and PHB-V (Progestar2; n = 52) devices were compared. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal device according to group (Day 0). Together with P4 device withdrawal (Day 8) all animals received 300IU of eCG i.m. (Novormon®, Schering-Plough) and 150 μg i.m. of D-cloprostenol (Croniben®, Biogenesis-Bagó, Buenos Aires, Argentina). On Day 9, all cows were treated with 1 mg of estradiol benzoate and the FTAI was performed 30 h later. The data were submitted to ANOVA (PROC GLM of SAS, SAS Institute, Cary, NC, USA), separating as causes of variance the effect of treatment, postpartum period (PPP1-45 until 70 days and PPP2-71 until 90 days), body condition score (BCS4, BCS5, and BCS6 using a 1 to 9 scale), cyclicity (cycling and noncycling) and clean-up bulls (5 bulls nominated with numbers 1 to 5). Significant differences were considered to have P < 0.05. In experiment 1, interactions were not significant, there was no effect of treatment on FTAI (DIB1: 66.7% v. Progestar1: 65.8%) or breeding season pregnancy rates (BSPR; DIB1: 95.0% v. Progestar1: 94.7%) and no effect of other variables on FTAI pregnancy rates (FTAIPR) and BSPR. In experiment 2 there was no effect of treatment on FTAIPR (DIB2: 61.6% v. Progestar2: 55.8%) and BSPR (DIB2: 94.2% v. Progestar2: 88.5%). As ANOVA indicated a significant effect of clean-up bulls on BSPR, Tukey test was used to split it up. A bull of the Progestar2 group had lower performance (41.7%) causing the difference (albeit nonsignificant) on BSPR. This experiment showed that Progestar and DIB had the same efficiency on pregnancy rate and both treatments have the potential to produce FTAIPR >50% and BSPR >90%. Fapesp-Fundação de Amparo à Pesquisa do Estado de São Paulo. CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

176 GENE EXPRESSION OF LUTEINIZING HORMONE RECEPTOR (LHR) ISOFORMS IN GRANULOSA CELLS OF FOLLICLES FROM NELLORE HEIFERS BEFORE, DURING, AND AFTER FOLLICULAR DEVIATION

2009 ◽  
Vol 21 (1) ◽  
pp. 187 ◽  
Author(s):  
C. M. Barros ◽  
R. L. Ereno ◽  
M. F. Machado ◽  
J. Buratini ◽  
M. F. Pegorer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Rna Extraction ◽  
Follicular Development ◽  
Sao Paulo ◽  
Luteinizing Hormone Receptor ◽  
São Paulo ◽  
Dominant Follicle ◽  
The Future ◽  
Group 2

During bovine follicular development, there is a phase known as follicular deviation in which the future dominant follicle grows faster than the other follicles and acquires LH receptors (LHR). In Nellore breed, deviation occurs 2.5 days after ovulation, and at this time, the dominant follicle has in average a diameter of 6.0 mm. Some authors believe that LHRs are present in the future dominant follicle before deviation and are essential for this process. However, others are convinced that LHRs are present only during or after follicular deviation. The aim of the present experiment was to evaluate the expression of 4 LHR isoforms (M1 to M4) in granulosa cells of follicles from Nellore heifers before, during, and after follicular deviation. At a random stage of the estrous cycle (D0), Nellore heifers (n = 21) received a progesterone intravaginal device (1.0 g, Primer®, Tecnopec, Sao Paulo, Brazil) and 2.5 mg of estradiol benzoate (EB, i.m., Estrogin®, Farmavet, Sao Paulo, Brazil). Eight days later (D8) PGF2α was administered (150 μg d-cloprostenol, i.m., Prolise®, ARSA S.R.L., Buenos Aires, Argentina), and the device was removed. Twenty-four hours after device removal, cows were treated with EB (1.0 mg, i.m.), and from this point in time, the growth of the dominant follicle growth was observed by ultrasonography (US, Aloka 900, Tokyo, Japan) every 12 h. The animals were allocated in 3 groups: Group 2 (G2, 2 days after ovulation, n = 7), Group 2.5 (G2.5, 2.5 days after ovulation, n = 7), and Group 3 (G3, 3 days after ovulation, n = 7), and were slaughtered 2, 2.5, and 3 days after ovulation, respectively, in order to remove the ovaries. The granulosa cells, obtained from ovarian follicles, were separated for total RNA extraction, and the gene expression of LHR isoforms was measured by semiquantitative RT-PCR. Since LHR expression was not detected in Group 2 (follicles with 4.5 to 6.7 mm), comparisons were performed between groups G2.5 and G3 by ANOVA. The LHR expression was detected only in 2 samples of Group G2 (7.0-mm follicles) and was significantly higher in Group G3 (63.6%; follicles from 8 to 14 mm, P < 0.05). In all samples that expressed LHR, the 4 isoforms were present. It is concluded that LHR expression is present in granulosa cells of follicles from Nellore heifers after follicular deviation. Support and fellowship from FAPESP (Sao Paulo, Brazil).We are grateful to Tecnopec (Sao Paulo, Brazil) for providing intravaginal devices used in the experiment.

8 EFFECT OF SEMEN TYPES (SEXED v. NONSEXED) ON CONCEPTION RATES OF GIROLANDO HEIFERS INSEMINATED IN FIXED-TIME AI

2010 ◽  
Vol 22 (1) ◽  
pp. 162
Author(s):  
J. Almeida ◽  
O. A. Resende ◽  
D. L. Silveira
Keyword(s):  
Body Condition Score ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
São Paulo ◽  
Device Removal ◽  
Conception Rates ◽  
Sexed Semen ◽  
Condition Score ◽  
Intravaginal Device

With the goal of optimizing protocols, fixed-time artificial insemination (FTAI) was performed under field conditions in a study to evaluate conception rates in Girolando (5/8) heifers (18 to 24 months of age) treated with estradiol benzoate (BE) to induce ovulation. Forty heifers were allocated to 2 groups with 20 animals each: GI-sexed semen (2 million spermatozoa/dose) and GII-nonsexed semen (20 million spermatozoa/dose), with body condition score (BCS) of 3.0 ± 0.14 (scale of 1 to 5), middleweight of 335 ± 38 kg, so that it was made for reutilization of progesterone device. On Day 0 (morning), the animals received 2.0 mg of BE (RIC-BE®,Tecnopec, São Paulo, Brazil), 0.075 mg of D-cloprostenol (Prolise®, ARSA S.R.L., Buenos Aires, Argentina), and an intravaginal device of 1 g of progesterone (Primer®, Tecnopec). On Day 8 (morning) the device was removed and 0.075 mg of D-cloprostenol was managed in the GI animals, then these animals received 1.0 mg of BE 24 h after device removal. The insemination was in the late afternoon of Day 10 with a 6-hour delay (4 to 6 pm). The GII animals received Primer previously used for 8 days (being used in the GI animals under the same protocol). After pregnancy diagnosis at 60 days, a third group was created (GIII) using the not-pregnant animals of GI and GII. In GIII, Primer from third use [disinfected in solution Kilol®-L 1:250 (Quinabra, São Paulo, Brazil) and rinsed with water, dried, and packed in plastic sacks and kept for 60 days] was used following the GI protocol with sexed semen. There were no significant differences (P > 0.05) in the conception rates of 40, 58, and 65% among groups GI (8/20), GII (11/20), and GIII (13/20). These results indicate very good conception rates for heifers, contrary to recommendations of commercial companies of sexed semen that alert for the conception rate for mated animals to be lower when using FTAI and mating protocols in mass, compared with that of AI after estrus observation.

178 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIME EMBRYO TRANSFER EMBRYO RECIPIENTS

2010 ◽  
Vol 22 (1) ◽  
pp. 247
Author(s):  
J. R. V. Pimentel ◽  
P. H. P. Miguez ◽  
A. Kehrle ◽  
E. H. Madureira ◽  
F. A. Braga ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Body Condition Score ◽  
Block Design ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
P Value ◽  
São Paulo ◽  
Condition Score ◽  
Intravaginal Device

Progestagen-releasing devices are commonly used for fixed-time artificial insemination and fixed-time embryo transfer (FTET). The aim of this study was to evaluate the efficacy of a new progesterone-releasing intravaginal device made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactone (PCL; Progestar®, Innovare, São Paulo, Brazil) to synchronize crossbredcows and heifers for FTET by determining ovulation, conception, and pregnancy rates. A total of 205 recipients ranging in age from 2 to 8 years (heifers, n = 77; non-suckled cows, n = 69; suckled cows, n = 59) were used on 3 different farms during February, March, and April 2008. The experimental design was a generalized block design (cyclicity, body condition score, postpartum interval, and animal category, i.e. heifers, suckled, or non-suckled cows), with 2 different synchronization treatments for FTET (Progestar® v. Crestar®). Cows were assigned to either Crestar® (norgestomet ear implants; Intervet Schering-Plough, Kenilworth, NJ, USA; n = 103) or Progestar® (PHB-V; n = 102) treatments. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal or auricular device according to their group (Day 0). Together with P4 device withdrawal (Day 8), all animals received 400 IU of eCG i.m. (Novormon®, Schering-Plough) and 500 μg i.m. of PGF2α, (Sincrocio®, Ouro Fino, Brasil). On Day 9, all cows were treated with 1 mg of estradiol benzoate (Innovare) and the innovulation was performed on Day 17. Embryos were maintained at 37°C during the innovulation period. The percentage of usable recipients (AR) based on the presence of a functional CL on the day of embryo transfer, conception rate (numbers of pregnant recipients of the total number transferred; CR), and pregnancy rate (numbers of pregnant recipients of the total number synchronized; PR) were determined by ultrasonography 23 days after embryo transfer. Data were submitted to ANOVA (PROC GLM, SAS Institute Inc., Cary, NC, USA) and significant differences were considered to have a P-value of <0.05. None of the interactions were significant and there was no effect of treatment on AR (72.8% v. 83.3%), CR (47.9% v. 42.7%), and PR (34.7% v. 35.0%) for Progestar® v. Crestar® groups, respectively. This experiment showed that Progestar® was as efficacious as Crestar® to synchronize crossbred cows and heifers for FTET. FAPESP-Fundação de Amparo à Pesquisa do Estado de São Paulo and CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

scholarly journals Treatment with PPARαAgonist Clofibrate Inhibits the Transcription and Activation of SREBPs and Reduces Triglyceride and Cholesterol Levels in Liver of Broiler Chickens

PPAR Research ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Lijun Zhang ◽  
Chunyan Li ◽  
Fang Wang ◽  
Shenghua Zhou ◽  
Mingjun Shangguan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Broiler Chickens ◽  
Target Genes ◽  
Nuclear Protein ◽  
Mrna Level ◽  
Control Group ◽  
Mrna Levels ◽  
Protein Levels ◽  
Cholesterol Levels ◽  
Regulation Mechanisms

PPARαagonist clofibrate reduces cholesterol and fatty acid concentrations in rodent liver by an inhibition of SREBP-dependent gene expression. In present study we investigated the regulation mechanisms of the triglyceride- and cholesterol-lowering effect of the PPARαagonist clofibrate in broiler chickens. We observed that PPARαagonist clofibrate decreases the mRNA and protein levels of LXRαand the mRNA and both precursor and nuclear protein levels of SREBP1 and SREBP2 as well as the mRNA levels of the SREBP1 (FASNandGPAM) and SREBP2 (HMGCRandLDLR) target genes in the liver of treated broiler chickens compared to control group, whereas the mRNA level ofINSIG2, which inhibits SREBP activation, was increased in the liver of treated broiler chickens compared to control group. Taken together, the effects of PPARαagonist clofibrate on lipid metabolism in liver of broiler chickens involve inhibiting transcription and activation of SREBPs and SREBP-dependent lipogenic and cholesterologenic gene expression, thereby resulting in a reduction of the triglyceride and cholesterol levels in liver of broiler chickens.

scholarly journals Survey of protozoan vector-borne diseases in dogs from Atlantic Rainforest fragment around Billings Dam, São Paulo, Brazil

2020 ◽  
Vol 50 (9) ◽  
Author(s):  
Roberta Carvalho de Freitas e Azevedo ◽  
Giovanna Stefani Nosberto Castelli ◽  
Ryan Emiliano da Silva ◽  
Jaciara de Oliveira Jorge Costa ◽  
Renata Tonhosolo ◽  
...  
Keyword(s):  
Animal Health ◽  
Companion Animals ◽  
Sao Paulo ◽  
Control Measures ◽  
Molecular Diagnostic ◽  
São Paulo ◽  
Vector Borne Diseases ◽  
Babesia Vogeli ◽  
Vector Borne ◽  
The Impact

ABSTRACT: Vector-borne diseases are currently one of the biggest public health concerns worldwide. Dogs, being the closest companion animals to humans, are considered the main reservoir of some of these diseases in the urban environment. Therefore, the study of the disease behavior in dogs can help to understand the disease affecting human health. Serological and molecular diagnoses of Babesia vogeli, Rangelia vitalli, Leishmania infantum, and other trypanosomatids, were performed by immunochromatographic and PCR assays, respectively, on dogs in a dog shelter located in an Atlantic Forest fragment near the Billings Dam, São Bernardo do Campo, São Paulo-Brazil. Our molecular diagnostic results showed a high prevalence of Babesia vogeli, at 20.9% (17/81). No other protozoan was detected in any of the tests. Determining the prevalence of major vector-borne diseases is essential to establish preventive and control measures for zoonotic diseases in animals kept in shelters, in order to minimize the impact of vector-borne diseases on animal health.

scholarly journals Machine Learning to predict tuberculosis in cattle from the state of Sao Paulo, Brazil

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
L E C Pereira ◽  
A S Ferraudo ◽  
A R Panosso ◽  
A A B Carvalho ◽  
L A Mathias ◽  
...  
Keyword(s):  
Machine Learning ◽  
Animal Health ◽  
Epidemiological Survey ◽  
Sao Paulo ◽  
The State ◽  
Machine Learning Algorithms ◽  
Test Model ◽  
São Paulo ◽  
Apparent Prevalence ◽  
Farm Type

Abstract Tuberculosis is a well-known and worldwide spread zoonosis. In Brazil 1.594.787 cases were confirmed cases since 2001, where, in Sao Paulo state, 8.226 deaths were reported. This study aims to present steps related to the use of machine learning algorithms for predictive analysis for bovine tuberculosis. For this, an application was made based on data from farms in state of São Paulo, Brazil, of an epidemiological survey, using a specific questionnaire, carried out on farms (n = 1,743). Response variable was presented by apparent prevalence of positive properties for disease, and predictors by (k = 77) predictors related to type of farm, type of lactation, number of animals on property. Application was organized according to following steps: division of data in training (75%) and testing (25%), pre-processing of predictors, learning and model evaluation. In the learning step, algorithm for adjusting gradient boosted trees models was used. The hyperparameters of algorithms were optimized by 10-fold cross-validation, to select those corresponding to best models. Models showed an accuracy of 88.07%, with an error in learning process equal to 3%. In the test / model validation procedure (n = 436), an error in 12% estimate was observed. Five important predictors were daily milk production, number of cows, type of farm, bovine breed and slaughter of adult animals. Proportion of false positives among all individuals whose response of interest was observed was 2.06%, and proportion of false negatives among those with a response of absent interest was 9.86%. It is hoped that, with increase in trained surveillance to detect the disease and availability of data, it will be possible to develop predictive models of machine learning with potential to efficiently assist professionals in disease control and assist in education program in animal health Key messages Predictive analyzes in health: application for tuberculosis in cattle from the state of Sao Paulo, Brazil. An infectious disease and zoonosis important to the world that needs support to develop means to control and consequently eradicate it.

225 ADDITION OF EQUINE CHORIONIC GONADOTROPIN TO A TRADITIONAL FOLLICLE STIMULATING HORMONE PROTOCOL FOR SUPEROVULATION OF BOS TAURUS BEEF COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 224 ◽  
Author(s):  
R. L. Davis ◽  
A. Arteaga ◽  
J. F. Hasler
Keyword(s):  
Treatment Group ◽  
South America ◽  
Bos Taurus ◽  
Animal Health ◽  
Bos Indicus ◽  
Treatment Protocol ◽  
Beef Cows ◽  
High Response Rate ◽  
Control Group ◽  
Embryo Production

This study examined the superovulatory responses of Bos taurus beef cows maintained in a commercial embryo transfer facility. Donors were superovulated 1 to 3 times each with either a traditional 8 injection FSH protocol (controls, n = 126) or 6 injections of FSH with the seventh or eighth FSH treatments replaced by 2 injections of eCG (treatment, n = 134). During the 5-month study, 132 donors were alternatively assigned to a control or treatment group for a single superovulation and an additional 62 animals were superovulated 2 (n = 58) or 3 times (n = 4) in a crossover design. Although 14 beef breeds were represented in the study, 87% of the cows were Angus, Red Angus, Polled Hereford, or Charolais. All donors were synchronized on Day 0 with a CIDR, 5 mg of oestradiol-17β and 100 mg of progesterone. Starting on Day 4 (p.m.), controls were injected twice daily for 4 days with descending doses of porcine FSH (Folltropin-V®, Bioniche Animal Health, Belleville, Ontario, Canada). Cows received 750 μg of cloprostenol (Estrumate®, Intervet Schering-Plough, Summit, NJ, USA) at the seventh FSH injection and the CIDR was removed at the eighth FSH injection. Based on previous experience with specific, individual animals, total FSH dose per donor ranged from 240 to 400 mg. However, 74% of treatments involved 380 mg for controls and 310 mg for treated donors that received eCG. In addition, donors that were superovulated more than once received the same FSH dose in the crossover, treatment-control design. In the treatment group, 200 IU of eCG (Pregnecol™ 6000, Bioniche Animal Health) was substituted for the seventh and eighth FSH injections. Inseminations were conducted on a timed AI basis, with one unit of semen 32 h and a second 48 h following CIDR removal. Results were analysed by ANOVA as shown in Table 1. Although more ova/embryos and unfertilized ova (UFO) were recovered in the control group, the control and treatment groups did not differ in the number of grade 1, 2, or 3 embryos or in the number of degenerate embryos. Previous superovulation studies in South America using eCG to replace the last 2 injections of FSH resulted in more total ova/embryos in Nelore cows but not heifers and in more embryos in Brangus and Sindhi cows. The mean embryo production for the control cows in this study was high and the addition of eCG in the protocol did not improve embryo production. Failure of eCG to increase the number of embryos for Bos taurus cows in this study compared with previous studies may be due to differences with Bos taurus versus Bos indicus breeds or differences in management factors between Canada and South America. The high response rate in the controls may also have contributed to the failure of any advantage of adding eCG to the treatment protocol. Table 1.Mean numbers (± SEM) of ova and embryos recovered from Bos taurus females superovulated with 2 different protocols

318 EFFICIENCY OF PROTOCOL P-36, ASSOCIATED WITH EQUINE CHORIONIC GONADOTROPIN OR LUTEINIZING HORMONE ADMINISTRATION, IN THE LAST DAY OF SUPERESTIMULATORY TREATMENT, IN NELLORE COWS

2011 ◽  
Vol 23 (1) ◽  
pp. 255 ◽  
Author(s):  
A. C. Oliveira ◽  
M. C. C. Mattos ◽  
M. R. Bastos ◽  
J. R. S. Gonçalves ◽  
L. H. Lunardi ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Fixed Time ◽  
Sao Paulo ◽  
Control Group ◽  
São Paulo ◽  
Embryo Production ◽  
Embryo Yield ◽  
Significant Difference ◽  
Equine Chorionic Gonadotropin ◽  
Viable Embryo

The objective of this study was to evaluate the use of pLH in replacement of eCG on the last day of P-36 superstimulatory treatment in Nellore donors. Recent studies demonstrated improvement in embryo production when the last 2 doses of FSH were replaced by eCG. However, consecutive use of eCG in bovine superstimulatory protocols may induce antibody against eCG, decreasing embryo production. Twenty-five Nellore cows were randomly allocated in 4 groups: P-36 (control), P-36/eCG, P-36/LH2, and P-36/LH4. All animals underwent 4 treatments in a cross-over design. Donors received an intravaginal device containing 1.0 g of progesterone (IVD, Primer®, Tecnopec, Sao Paulo, Brazil) and oestradiol benzoate (2.0 mg, IM; Estrogin®, Farmavet, Sao Paulo, Brazil) at a random stage of the oestrous cycle (D0). Cows from the control group were superestimulated with decreasing doses of pFSH (133 mg, IM; Folltropin-V®, Bioniche, Ontario, Canada; D5-8). In the P-36/eCG group, the last 2 doses of pFSH were replaced by 2 doses of eCG (200 IU each dose, IM; Folligon®, Intervet, Boxmeer, the Netherlands). In the P-36/LH2 and P-36/LH4 groups, the last 2 doses of pFSH were replaced by 2 doses of 1 and 2 mg of pLH, respectively (IM; Lutropin®, Bioniche). All animals were treated with prostaglandin F2α (150 μg d-cloprostenol, IM; Prolise®, Tecnopec) on D7, and the IVD was removed 36 h after. Ovulation was induced with 12.5 mg of pLH (IM) on D9, and all animals received fixed-time artificial insemination 12 and 24 h after pLH. Embryo flushing was performed on D16. Data were analysed by ANOVA (Proc Mixed, SAS). There was a significant difference in the number of corpus luteum in the eCG group (19.2 ± 2.4) when compared with the LH2 (12.7 ± 2.0) and LH4 groups (12.3 ± 1.5; P < 0.05). In addition, there was a tendency of lower ovulation rate in the LH2 group as compared with the eCG group (50.6 and 67.8%, respectively; P = 0.06). However, there was no difference in viable embryo yield among groups P-36 (3.3 ± 0.7), P-36/eCG (4.5 ± 0.5), P-36/LH2 (3.7 ± 0.8), and P-36/LH4 (4.2 ± 1.0); P > 0.05. In conclusion, eCG can be replaced by pLH (4.0 mg), in the last day of P-36 protocol, without affecting the production of viable embryos in Nellore cows. The authors acknowledge FAPESP (Sao Paulo, Brazil) for funding and fellowships for A. C. S. Oliveira, M. C. C. Mattos, and M. R. Bastos.

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