269 EFFECT OF DIFFERENT COLLECTION FREQUENCY INTERVALS ON THE QUALITY OF SEMEN OF FRENCH BULLDOGS

2015 ◽  
Vol 27 (1) ◽  
pp. 223
Author(s):  
C. B. Deorce ◽  
F. L. G. Leite ◽  
B. Loureiro
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Repeated Measure ◽  
Normal Morphology ◽  
The Third ◽  
Counting Chamber ◽  
Increased Sensitivity ◽  
Digital Manipulation

Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculiarities, low libido, and increased sensitivity to stress could cause further reductions in sperm count. The objective was to compare effects of semen collection at 24- versus 48-h intervals on semen quality of French Bulldogs. Five purebred French Bulldogs, 19 to 48 months old, were subjected to 5 semen collections (24 h apart). After a 30-day rest, collections were repeated, but the interval between collections was 48 h. Semen was collected (all 3 fractions) by digital manipulation without female stimuli. Volume was measured in a 20-mL syringe. Sperm concentration was determined with a Neubauer counting chamber. Motility and vigor were evaluated with a coverslipped drop of semen on a slide (preheated to 37°C). Motility was expressed as a percentage of motile sperm, and vigor was classified on a scale of 1 to 5. Morphology was evaluated by the panoptic method; 100 cells were counted and results expressed as the percentage of normal or defective cells. Effects of collection interval were analysed using PROC MIXED of SAS (animal as subject and collection as a repeated measure), with collections 2 to 5 compared with collection 1 (using the DIFF option). For collection every 24 h, the third, fourth, and fifth sperm collections were lower than the first collection for volume (10.4 ± 1.1, 8.3 ± 1.1, 5.6 ± 1.1, 3.5 ± 1.1, 2.4 ± 1.1 mL), concentration (437 ± 24, 448 ± 24, 370 ± 24, 322 ± 27, 258 ± 31 ×106 sperm mL–1), vigor (4.6 ± 0.2, 4.2 ± 0.2, 3.6 ± 0.2, 3.7 ± 0.2, 3 ± 0.2), and morphologically normal sperm (82 ± 2.2, 83 ± 2.2, 72 ± 2.2, 68 ± 2.5, 66 ± 2.9%). However, when the interval was increased to 48 h, only the fourth and fifth collections were lower (P < 0.05) than the first for volume (11.8 ± 1.1, 10.2 ± 1.1, 8.8 ± 1.1, 6.6 ± 1.1, 2.5 ± 1.1 mL), concentration (447 ± 24, 410 ± 24, 407 ± 24, 322 ± 24, 241 ± 31 ×106 sperm mL–1), vigor (5 ± 0.2, 4.8 ± 0.2, 4.4 ± 0.2, 4.2 ± 0.2, 4 ± 0.2), and sperm with normal morphology (92 ± 2.2, 90 ± 2.2, 87 ± 2.2, 80 ± 2.2, 81 ± 2.9%). Motility decreased (P < 0.05) following the fourth collection at 24-h intervals and decreased (P < 0.05) after the third collection at 48-h intervals. With a 24-h interval, 4 dogs had <60% motility lower at the fifth collection, whereas only 2 dogs had motility <60% on the fifth collection at 48-h intervals. In conclusion, semen collected at 48-h intervals was of better quality than semen collected at 24-h intervals.

scholarly journals Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

2015 ◽  
Vol 59 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Andrzej Jurczak ◽  
Tomasz Janowski
Keyword(s):  
Vitamin E ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Control Group ◽  
Organic Selenium ◽  
Healthy Dogs ◽  
Manual Manipulation ◽  
Motility Parameters

Abstract Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

scholarly journals The Association Between Abstinence Period and Semen Parameters: Results in Normal Samples and Different Sperm Pathology

2021 ◽  
Author(s):  
Min Xie ◽  
Silvan Hämmerli ◽  
Kerstin Blickenstorfer ◽  
Brigitte Leeners
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sexual Abstinence ◽  
Normal Morphology ◽  
Few Data ◽  
Total Motile Sperm Count ◽  
Abstinence Period

Abstract Purpose: The length of sexual abstinence seems to influence sperm quality. However, few data on the relevance of abstinence time in pathological sperm samples are available. With our study, we look for associations of abstinence length and semen quality. Methods: We studied semen samples from 4423 men undergoing fertility evaluation. Sperm concentration, percentage of progressively motile spermatozoa, total motile sperm count, percentage of spermatozoa with normal morphology, were compared after each day and 0-2, 3-7 and >7 days of abstinence. Results: We found that a longer abstinence time was related to higher sperm concentration in normal semen samples (P<0.001) and in semen samples with any sperm pathology (P<0.001, P=0.004) with the exception of oligozoospermia (P=0.125). Longer abstinence time was also associated with significantly reduced progressive motility in normal samples (P<0.001) and in cases of teratozoospermia (P<0.001). In normal samples a higher percentage of sperm were morphologically normal after a shorter abstinence period (P=0.03); in oligoasthenoteratozoospermia (OAT) samples, this was the case after a longer abstinence period (P=0.013). Conclusion: A longer abstinence time is associated with higher sperm concentration, whereas sperm motility is optimal after shorter abstinence times; results on morphology are controversial. The recommendation on abstinence time needs to be adjusted in relation to the parameter that needs to be improved.

scholarly journals The Association Between Abstinence Period And Semen Parameters: Results In Normal Samples And Different Sperm Pathology

2021 ◽  
Author(s):  
Min Xie ◽  
Silvan Haemmerli ◽  
Kerstin Blickenstorfer ◽  
Brigitte Leeners
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sexual Abstinence ◽  
Normal Morphology ◽  
Few Data ◽  
Total Motile Sperm Count ◽  
Abstinence Period

Abstract Purpose: The length of sexual abstinence seems to influence sperm quality. However, few data on the relevance of abstinence time in pathological sperm samples are available. With our study, we look for associations of abstinence length and semen quality. Methods: We studied semen samples from 4423 men undergoing fertility evaluation. Sperm concentration, percentage of progressively motile spermatozoa, total motile sperm count, percentage of spermatozoa with normal morphology, were compared after each day and 0-2, 3-7 and >7 days of abstinence.Results: We found that a longer abstinence time was related to higher sperm concentration in normal semen samples (P<0.001) and in semen samples with any sperm pathology (P<0.001, P=0.004) with the exception of oligozoospermia (P=0.125). Longer abstinence time was also associated with significantly reduced progressive motility in normal samples (P<0.001) and in cases of teratozoospermia (P<0.001). In normal samples a higher percentage of sperm were morphologically normal after a shorter abstinence period (P=0.03); in oligoasthenoteratozoospermia (OAT) samples, this was the case after a longer abstinence period (P=0.013).Conclusion: A longer abstinence time is associated with higher sperm concentration, whereas sperm motility is optimal after shorter abstinence times; results on morphology are controversial. The recommendation on abstinence time needs to be adjusted in relation to the parameter that needs to be improved.

scholarly journals Influence of environmental factors on the qualitative and quantitative parameters of male ejaculate (literature review)

2019 ◽  
Vol 23 (3) ◽  
pp. 530-534
Author(s):  
S.V. Khmil ◽  
O.Yu. Mayorova ◽  
I.V. Dudchuk
Keyword(s):  
Environmental Factors ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Toxic Substances ◽  
Normal Morphology ◽  
Qualitative And Quantitative ◽  
Literary Sources ◽  
The Impact

Today it is important to study the problem of male infertility, as well as to identify the factors of its occurrence. The purpose of the work is to evaluate the overall picture of the quality of ejaculate during the last 50 years and to determine the reasons for such changes. More than 40 literary sources were published for thorough analysis in the period 1984–2018 (the share of new references is 20%). There was a decrease in sperm concentration by 1.5% / year, total sperm count — 1.6% / year, total sperm motility — 0.4% / year, decrease in the percentage of progressive motility sperm by 5.5% / year and with normal morphology 2.2% / year. One of the main reasons for the deterioration of sperm quality is the impact of negative environmental factors — chemical and radioactive contamination, as well as electromagnetic radiation. Among the most toxic substances are the heavy metals, pesticides, bisphenol A, phthalates, polychlorinated biphenyls, dioxin and its derivatives. Thus, over the last 50 years there has been a decline in the qualitative and quantitative indices of ejaculate men worldwide. One of the reasons is the worsening environmental situation.

scholarly journals Effect of L-Carnitine Supplementation on Boar Semen Quality

2007 ◽  
Vol 76 (4) ◽  
pp. 595-600 ◽  
Author(s):  
E. Jacyno ◽  
A. Kołodziej ◽  
M. Kamyczek ◽  
M. Kawęcka ◽  
K. Dziadek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Carnitine Supplementation ◽  
Boar Semen ◽  
Positive Effect ◽  
Plasma Activity

The effect of addition of L-carnitine on boar semen quality was studied in 5 Pietrain boars at age 1.5 - 2.0 years. The boars received 500 mg of L-carnitine per day for 5 weeks. During this period, their ejaculates were collected once a week and evaluated for quality. The control ejaculates had been collected before the application of L-carnitine. It was found that the addition of L-carnitine to the boars' feed had a positive effect on the quality of boar semen. The total ejaculate volume and sperm-rich fraction volume increased by 11% and 10%, respectively; the total ejaculate sperm count increased by 11.5% (P < 0.05). Also, the number of spermatozoa with major and minor morphological changes decreased and seminal plasma activity of AspAT was significantly reduced (P < 0.01). Sperm concentration and motility, as well as normal acrosome sperm percentage, did not increase considerably. The positive effect of L-carnitine on boar semen quality was observable as early as after one week of its application.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

scholarly journals Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men

2021 ◽  
Vol 18 (4) ◽  
pp. 1742
Author(s):  
Thea Emily Benson ◽  
Anne Gaml-Sørensen ◽  
Andreas Ernst ◽  
Nis Brix ◽  
Karin Sørig Hougaard ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Fertility ◽  
Parental Education ◽  
Negative Binomial ◽  
Semen Quality ◽  
Sperm Count ◽  
Smoking Status ◽  
Sperm Concentration ◽  
First Trimester ◽  
Negative Binomial Regression Model

Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.

scholarly journals What Is the Impact of Excessive Iodine and Semen Quality in Fertile Men of China: An Association Study 

2021 ◽  
Author(s):  
Ning Wang ◽  
Fang Lv ◽  
Ge Yu ◽  
Cuige Shi ◽  
Shangming Wang ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Male Reproduction ◽  
Semen Parameters ◽  
Human Thyroid ◽  
Urinary Iodine Concentration ◽  
The Impact

Abstract Background: Iodine is an essential element for hormones synthesized by the human thyroid gland. Thyroid hormone deficiency affects all body tissues, including multiple endocrine changes that alter growth hormones, corticotrophin, glucocorticoids, and gonadal function. In many countries, subfertility is an important problem affecting about 10–15% of all couples trying to conceive. Male subfertile factors have accounted for 25–30% of all cases in their studies. The aim of this study was to study the correlation between urinary iodine concentration (UIC) and semen quality of fertile male in China.Methods: semen and urine samples were collected from males who attended a survey for the reproductive physiological constants of male and female in China. All participants were fertile and they should be free from diseases of male reproductive system and thyroid related diseases. We studied the correlation between UIC and semen quality parameters in fertile males and the effect of iodine concentration on male reproduction. Participants were divided into six groups based on UIC. We measured semen parameters and UIC. Semen parameters or time-to-pregnancy (TTP) were analyzed by Spearman correlation, linear regression and multivariable logistic regression. Results: 1089 men were recruited in the study. The mean and median age of participants were 27.46±0.12 and 27±3.88 years, respectively. UIC of 274 (25.16%) men was lower than the UIC of WHO recommended. UIC of 405 men located in the region of WHO recommended. 62.35% of them were not located in the optimal region. Semen concentration and total sperm count were found to decrease when UIC increases when the UIC≥100 ug/L. Conclusions: A male with deficient UIC or excessive UIC has more risk of lower semen quality such as sperm concentration and total sperm count. In addition, if a male with deficient UIC or excessive UIC, his wife has longer TTP than the males who had normal UIC. Deficient UIC associated with lower semen quality and TTP than excessive UIC.

scholarly journals REPRODUCING QUALITIES OF RAMS OF THE MOLDOVAN TYPE KARAKUL BREED

2020 ◽  
Vol 59 ◽  
pp. 92-96
Author(s):  
D. Rotari
Keyword(s):  
Sperm Count ◽  
Sperm Concentration ◽  
Animal Husbandry ◽  
Sperm Production ◽  
Livestock Farming ◽  
High Quality ◽  
Average Percentage ◽  
Reproductive Ability ◽  
First Time

Issues related to the reproduction of animals have been and remain one of the most complex and relevant problems of biology and are constantly finding a direct and effective way out into livestock farming practice. The rational use of breeding sheep as producers is limited by the lack of standard, objective methods and methods for the timely assessment of their reproductive ability. The article presents the results of evaluating the sperm production of rams-producers. For the first time, the freshly obtained sperm of rams of Moldavian type producers of the Karakul breed was evaluated using the macroscopic method - ejaculate volume, color and smell, as well as the microscopic method - motility, sperm concentration in the ejaculate, total sperm count in the ejaculate sperm movements (VAP; VSL and VCL) as well as the percentage of abnormal forms of sperm. The experiments were carried out on sheep producers grown on a pedigree farm of the experimental farm of the Moldavian Scientific and Practical Institute of Biotechnology in Animal Husbandry and Veterinary Medicine. As a result of the studies, it was found that the ejaculate volume on average was 0.99 ± 0.04 ml, motility was 0.95 ± 0.02 and sperm concentration 1.51 ± 0.14 billion/ml. The percentage of pathological forms in sperm averaged 13.72 ± 0.61, an indicator that characterizes the high quality of sperm. The average quality indicator of ejaculates obtained from ram-producers of the Moldavian type of the Karakul breed corresponds to physiological standards for the Karakul breed. The average percentage of pathological forms of sperm found in ejaculates indicates that the rams were in good conditions of feeding and keeping. According to research, we can say that the Moldovan type of Karakul rams can be successfully used to obtain high quality ejaculates suitable for freezing sperm at 196°C.

scholarly journals Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

2013 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Rafał Strzeżek ◽  
Krystyna Filipowicz ◽  
Marta Stańczak ◽  
Władysław Kordan
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Spectrophotometric Analysis ◽  
Normal Morphology ◽  
Additional Tool ◽  
Reduction Test ◽  
Highly Correlated

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

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