scholarly journals A review of the physiology of the canine digestive tract related to the development ofin vitrosystems

1998 ◽  
Vol 11 (1) ◽  
pp. 45-69 ◽  
Author(s):  
Marianne Smeets-Peeters ◽  
Tim Watson ◽  
Mans Minekus ◽  
Robert Havenaar
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Healthy Adult ◽  
Human Beings ◽  
Ph Values ◽  
Transit Times ◽  
Food And Nutrition ◽  
Different Types

AbstractFood and nutrition studies in animals and human beings often meet with technical difficulties and sometimes with ethical questions. An alternative to research in living animals is the dynamic multicompartmentalin vitromodel for the gastrointestinal tract described by Minekuset al.(1995) and Havenaar & Minekus (1996). The dynamic conditions that are simulated in this model are peristaltic movements, transit times, pH responses, secretion of enzymes and electrolytes and absorption of nutrients and water. To obtain data for anin vitromodel of the dog gastrointestinal tract, the literature was surveyed for physiological responses to different types of dog food. These included: values of enzyme activities, electrolyte concentrations, gastric emptying and intestinal transit times, pH values, secretion and composition of bile and absorption rates in different parts of the dog gastrointestinal tract. The review focuses on research carried out on healthy, adult dogs of 10–20 kg and on parameters related to the oral cavity, stomach and small intestine. This literature research gives sufficient data on the physiology of the canine digestive tract for the development of anin vitrodynamic model that adequately simulates the functions of the stomach and small intestine of the dog.

Insights into bread melanoidins: fate in the upper digestive tract and impact on the gut microbiota using in vitro systems

2015 ◽  
Vol 6 (12) ◽  
pp. 3737-3745 ◽  
Author(s):  
Cynthia Helou ◽  
Sylvain Denis ◽  
Madeleine Spatz ◽  
David Marier ◽  
Véronique Rame ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gut Microbiota ◽  
Digestive Tract ◽  
Batch Fermentation ◽  
Dramatic Decrease ◽  
Upper Digestive Tract ◽  
In Vitro Systems

Bread melanoidins are partially degraded in the small intestine and induce a dramatic decrease of enterobacteria during batch fermentation.

GASTROINTESTINAL HYPOMOTILITY IN MAGNESIUM-DEFICIENT SHEEP

1980 ◽  
Vol 60 (2) ◽  
pp. 293-301 ◽  
Author(s):  
L. BUENO ◽  
J. FIORAMONTI ◽  
E. GEUX ◽  
Y. RAISSIGUIER
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Electrical Activity ◽  
Digestive Tract ◽  
Experimental Period ◽  
Proximal Colon ◽  
Slow Waves ◽  
Deficient Diet ◽  
Intravenous Infusions ◽  
Spiking Activity

The electrical activity of the gastrointestinal tract and gallbladder was recorded in four sheep fed a Mg-deficient diet during 10 to 15 days. The mitigating effect of intravenous infusions of MgCl2 was tested at the end of the experimental period in animals presenting hypomagnesemia. Motility of the reticulo-rumen remained unchanged in Mg-deficient sheep except that there was no postprandial increased frequency of contractions. By contrast, the contractions of gallbladder, cecum and proximal colon were reduced in both amplitude and frequency. The amplitude but not the frequency of the antro-duodenal slow-waves was reduced. The amplitude of the regular spiking activity of the small intestine was reduced as well as the number of complexes produced per day. The activity of the spiral colon was correlated to the blood magnesium concentrations but Mg infusion was unable to restore immediately the motor profile of the rest of the gut to its intitial level. This was done within 2–3 days by changes in the diet in three of the four animals. It is concluded that the motility of the whole digestive tract, including the reticulo-rumen, is modified on a Mg-deficient diet and that hypomagnesemia, involved in the atony of the spiral colon, is only one of the factors responsible for the hypomotility.

In vitro Gastrointestinal Models for Prebiotic Carbohydrates: A Critical Review

2019 ◽  
Vol 25 (32) ◽  
pp. 3478-3483 ◽  
Author(s):  
Oswaldo Hernandez-Hernandez
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Brush Border ◽  
Critical Review ◽  
In Vitro Digestibility ◽  
Human Gastrointestinal Tract ◽  
Intestinal Brush Border ◽  
Small Intestinal ◽  
Existing Data

Background: In the last decade, various consortia and companies have created standardized digestion protocols and gastrointestinal simulators, such as the protocol proposed by the INFOGEST Consortium, the simulator SHIME, the simulator simgi®, the TIM, etc. Most of them claim to simulate the entire human gastrointestinal tract. However, few results have been reported on the use of these systems with potential prebiotic carbohydrates. Methods: This critical review addresses the existing data on the analysis of prebiotic carbohydrates by different in vitro gastrointestinal simulators, the lack of parameters that could affect the results, and recommendations for their enhancement. Results: According to the reviewed data, there is a lack of a realistic approximation of the small intestinal conditions, mainly because of the absence of hydrolytic conditions, such as the presence of small intestinal brush border carbohydrases that can affect the digestibility of different carbohydrates, including prebiotics. Conclusion: There is a necessity to standardize and enhance the small intestine simulators to study the in vitro digestibility of carbohydrates.

Interaction between sites of magnesium absorption in the digestive tract of the sheep

1976 ◽  
Vol 27 (3) ◽  
pp. 437 ◽  
Author(s):  
FM Tomas ◽  
BJ Potter
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Magnesium Chloride ◽  
Basal Diet ◽  
Faecal Excretion ◽  
Intestinal Segments ◽  
Effect Of Treatment ◽  
The Mean ◽  
Plasma Magnesium

The effect of magnesium chloride infusion to different sites in the gastrointestinal tract of sheep upon the net absorption of magnesium from different regions of the digestive tract has been examined. Four Merino wethers were each prepared with cannulas sited in the rumen, in the duodenum adjacent to the pylorus and in the terminal ileum. The basal diet provided 18.45 mmoles magnesium/ day and an additional 65 mmoles magnesium/day as magnesium chloride was continuously infused into (A) the rumen, (B) the rumen and duodenum in equal portions, (C) the duodenum and (D) the terminal ileuni. A continuous infusion of Cr-EDTA to the ruinen enabled digcsta and magnesium flow rates to be estimated from digesta samples obtained from the intestinal cannulas at 4 hr intervals over 3 days. For treatments A, B, C and D respectively, the mean net absorption of magnesium (mmoles/day) from the rumen was 20.4, 11.4, 1.4 and 3.4; from the small intestine –0.5, 1.7, –5.1 and –9.8; from the large intestine 4.6, 2.2, 12.7 and 12.3; and from the total gastrointestinal tract 24.6, 15.4, 9.1 and 4.9. In each case the effect of treatment was significant. The total net absorption of magnesium caudal to the pylorus was unaffected by treatment. Plasma magnesium levels were reduced during post-ruminal infusion of magnesium, but these changes were not obviously linked to the changed net absorption from the intestinal segments. The urinary and faecal excretion of magnesium, but not the magnesium balance, was strongly related to the total net absorption of magnesium. The results emphasize the major contribution of the stomach to the gastrointestinal net absorption of magnesium and show that although the amount absorbed from this region may influence separately the net absorption from the large and small intestine, it does not appear to influence the overall intestinal net absorption of magnesium.

Ileal and hindgut fermentation in the growing pig fed a human-type diet

2020 ◽  
Vol 124 (6) ◽  
pp. 567-576 ◽  
Author(s):  
Anna M. E. Hoogeveen ◽  
Paul J. Moughan ◽  
Edward S. de Haas ◽  
Paul Blatchford ◽  
Warren C. McNabb ◽  
...  
Keyword(s):  
Body Weight ◽  
Organic Matter ◽  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Taxonomic Composition ◽  
Human Type ◽  
Growing Pig ◽  
Microbial Inocula

AbstractDietary fibre fermentation in humans and monogastric animals is considered to occur in the hindgut, but it may also occur in the lower small intestine. This study aimed to compare ileal and hindgut fermentation in the growing pig fed a human-type diet using a combined in vivo/in vitro methodology. Five pigs (23 (sd 1·6) kg body weight) were fed a human-type diet. On day 15, pigs were euthanised. Digesta from terminal jejunum and terminal ileum were collected as substrates for fermentation. Ileal and caecal digesta were collected for preparing microbial inocula. Terminal jejunal digesta were fermented in vitro with a pooled ileal digesta inoculum for 2 h, whereas terminal ileal digesta were fermented in vitro with a pooled caecal digesta inoculum for 24 h. The ileal organic matter fermentability (28 %) was not different from hindgut fermentation (35 %). However, the organic matter fermented was 66 % greater for ileal fermentation than hindgut fermentation (P = 0·04). Total numbers of bacteria in ileal and caecal digesta did not differ (P = 0·09). Differences (P < 0·05) were observed in the taxonomic composition. For instance, ileal digesta contained 32-fold greater number of the genus Enterococcus, whereas caecal digesta had a 227-fold greater number of the genus Ruminococcus. Acetate synthesis and iso-valerate synthesis were greater (P < 0·05) for ileal fermentation than hindgut fermentation, but propionate, butyrate and valerate synthesis was lower. SCFA were absorbed in the gastrointestinal tract location where they were synthesised. In conclusion, a quantitatively important degree of fermentation occurs in the ileum of the growing pig fed a human-type diet.

Evaluation of moist heat treatment of canola meal on digestion in the rumen, small intestine, large intestine and total digestive tract of steers

1995 ◽  
Vol 75 (3) ◽  
pp. 279-283 ◽  
Author(s):  
S. A. Moshtaghi Nia ◽  
J. R. Ingalls
Keyword(s):  
Heat Treatment ◽  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Canola Meal ◽  
Glucosinolate Content ◽  
Moist Heat ◽  
Rumen Degradation ◽  
Heat Treated

The effect of heat treatment on canola meal (CM) dry matter (DM) and nitrogen (N) degradability in the rumen and digestion in the small and large intestines and total digestive tract of steers was determined. CM was moist-heat-treated at 127 °C for 15 and 45 min. The degradation of DM and N was estimated using small nylon bags in the rumen for 0.1, 4, 8, 12, 16, 24, 48 and 72 h. The mobile bag technique with an in vitro incubation in an acid-pepsin solution was used with a separate set of bags to estimate the digestion of each nutrient in the small and large intestines as well as the total digestive tract for rumen incubation times of 8, 16 and 24 h. Chemical analysis of heated CM showed a significant reduction in soluble N, sucrose and glucosinolate content while the concentration of ADIN increased. Treatment significantly reduced the degradation of DM and N in the rumen while the digestibility of DM and N in the small intestine increased with both treatments proportional to the decreased rumen degradation. Heat treatment had no effect on DM and N digestibility at the large intestine site. Total tract disappearance of DM and N were not affected by 15 min heating; however, after 45 min heating, DM and N disappearances were reduced. Moist heat treatment was effective in reducing rumen disappearance of CM nitrogen while it increased the N digestion in the small intestine. Key words: Heat treatment, canola meal, ruminant intestine, protein digestion

scholarly journals Localization of amidating enzymes (PAM) in rat gastrointestinal tract.

1993 ◽  
Vol 41 (11) ◽  
pp. 1617-1622 ◽  
Author(s):  
A Martínez ◽  
M A Burrell ◽  
M Kuijk ◽  
L M Montuenga ◽  
A Treston ◽  
...  
Keyword(s):  
Small Intestine ◽  
In Situ Hybridization ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Endocrine Cells ◽  
Regulatory Peptides ◽  
Serial Sections ◽  
Gastric Glands ◽  
Amidating Enzymes

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.

COMPARISON OF THE METABOLISM OF TESTOSTERONE UNDECANOATE AND TESTOSTERONE IN THE GASTROINTESTINAL WALL OF THE RAT IN VITRO AND IN VIVO

1977 ◽  
Vol 86 (1) ◽  
pp. 216-224 ◽  
Author(s):  
J. Geelen ◽  
A. Coert ◽  
R. Meijer ◽  
J. van der Vies
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Portal Vein ◽  
Oral Administration ◽  
Small Extent ◽  
Great Similarity ◽  
Testosterone Undecanoate ◽  
Different Parts

ABSTRACT The metabolism of testosterone undecanoate (TU) and testosterone (T) is studied in the gastrointestinal wall of the rat in vitro. A comparison is made with the in vivo metabolism of these compounds in the rat. The major metabolite first appearing during incubation of TU with the small intestine is T. Incubation of TU or T with the small intestine reveals a great similarity between the metabolite patterns obtained. This is also the case with the patterns derived from portal vein plasma upon oral administration of TU and T. Incubation of different parts of the gastrointestinal tract with TU or T shows that the greatest metabolic activity is located in the wall of the small intestine. Unlike T, TU is metabolized only to a small extent in the wall of the stomach and the large intestine.

Microorganisms in the gastrointestinal tract of the rat prevent absorption of the mutagen–carcinogen 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole

1993 ◽  
Vol 39 (9) ◽  
pp. 841-845 ◽  
Author(s):  
Xue Bin Zhang ◽  
Yoshiyuki Ohta
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Intestinal Bacteria ◽  
Gastrointestinal Absorption ◽  
Aqueous Methanol ◽  
Freeze Dried

The extent to which lactic acid bacteria, intestinal bacteria, and yeast from the gastrointestinal tract of rats suppress the absorption of 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole (Trp-P-1) was investigated. Trp-P-1 was absorbed from the small intestine very rapidly, but in the stomach it was slowly absorbed, requiring 1 or 2 h after administration. When mixtures of Trp-P-1 and freeze-dried microorganisms were administered to rats for 1 h, the amounts of Trp-P-1 absorbed from the small intestine were significantly reduced, and the levels of Trp-P-1 in blood decreased by 40.4–64.7% compared with a control in which only Trp-P-1 was administered. There were no significant differences between the organisms used. In vitro, freeze-dried cells of the strains tested bound 51-97% of Trp-P-1. The Trp-P-1 bound to cells was effectively extracted by aqueous methanol, ethanol, ammonia (50 g/L), and solutions of MgCl2 and CaCl2 (100 mM/mL), but little was extracted by water and solutions of KCl, NaCl, and buffers at various pH values.Key words: Trp-P-1, gastrointestinal absorption, binding, intestinal bacteria, mutagen–carcinogen.

Comparative gross morphology of the digestive tract in ten Didelphidae marsupial species

Mammalia ◽  
2004 ◽  
Vol 68 (1) ◽  
Author(s):  
R.T. Santori ◽  
D. Astua De Moraes ◽  
Rui Cerqueira
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Large Intestine ◽  
Food Habits ◽  
Relative Size ◽  
Marsupial Species

Natural diets of Didelphidae species vary in the amounts of invertebrates, fruits and small vertebrates eaten. We investigated the digestive morphology of ten species of didelphid marsupials varying in food habits. The purpose was to describe and to compare the shape and relative size of the digestive tract portions among species studied and relate them to food habits. The form of the gastrointestinal tract in this family is simple, with a unilocular stomach, small intestine, large intestine and caecum.

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