Microorganisms in the gastrointestinal tract of the rat prevent absorption of the mutagen–carcinogen 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole

1993 ◽  
Vol 39 (9) ◽  
pp. 841-845 ◽  
Author(s):  
Xue Bin Zhang ◽  
Yoshiyuki Ohta
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Intestinal Bacteria ◽  
Gastrointestinal Absorption ◽  
Aqueous Methanol ◽  
Freeze Dried

The extent to which lactic acid bacteria, intestinal bacteria, and yeast from the gastrointestinal tract of rats suppress the absorption of 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole (Trp-P-1) was investigated. Trp-P-1 was absorbed from the small intestine very rapidly, but in the stomach it was slowly absorbed, requiring 1 or 2 h after administration. When mixtures of Trp-P-1 and freeze-dried microorganisms were administered to rats for 1 h, the amounts of Trp-P-1 absorbed from the small intestine were significantly reduced, and the levels of Trp-P-1 in blood decreased by 40.4–64.7% compared with a control in which only Trp-P-1 was administered. There were no significant differences between the organisms used. In vitro, freeze-dried cells of the strains tested bound 51-97% of Trp-P-1. The Trp-P-1 bound to cells was effectively extracted by aqueous methanol, ethanol, ammonia (50 g/L), and solutions of MgCl2 and CaCl2 (100 mM/mL), but little was extracted by water and solutions of KCl, NaCl, and buffers at various pH values.Key words: Trp-P-1, gastrointestinal absorption, binding, intestinal bacteria, mutagen–carcinogen.

Stability of the bacteria-bound zearalenone complex in ruminal fluid and in simulated gastrointestinal environment in vitro

2008 ◽  
Vol 1 (4) ◽  
pp. 463-467 ◽  
Author(s):  
V. Niderkorn ◽  
H. Boudra ◽  
D. Morgavi
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Biological Effects ◽  
Streptococcus Thermophilus ◽  
Farm Animals ◽  
Ruminal Fluid ◽  
The Stability ◽  
Partial Dissociation

Zearalenone (ZEA), an oestrogenic secondary metabolite of Fusarium, is a common feed contaminant. Lactic acid bacteria are capable to bind ZEA and this property could be used to limit its negative biological effects on farm animals. The aim of this work was to examine the stability of a lactic acid bacterium, Streptococcus thermophilus RAR1-ZEA complex in ruminal fluid and in conditions simulating compartments of the gastrointestinal tract. The lactic acid bacteria-ZEA complex was 70% stable in ruminal fluid incubations for up to 18 h, and about 50% of ZEA initially complexed still remained bound after incubation in pepsin, lysozyme, pancreatin and bile either alone or sequentially. However, the release of ZEA was predominantly caused by washing, except for bile which produced a partial dissociation of the complex (P<0.05). Addition of free ZEA to ruminal fluid resulted in instantaneous binding of about 70% of the toxin. ZEA bound to ruminal fluid was even more stable than lactic acid bacteria-ZEA in in vitro ruminal fermentations and in simulated gastrointestinal tract juices. It is concluded that complexation of ZEA in ruminal fluid could help protect ruminants against the toxin, and that the use of selected strains of lactic acid bacteria efficiently binding ZEA to limit its toxic effects could be more advantageous in monogastric animals.

scholarly journals Feeding Of Calcium and Protein Macroparticle Or Microparticle With Bitter Mustard Root On Intestinal Bacteria Population And Nutrient Intake In Quail

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Lilik Krismiyanto ◽  
Nyoman Sutama ◽  
Vitus Dwi Yunianto ◽  
Fajar Wahyono ◽  
Citra Ardelia ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Calcium Intake ◽  
Nutrient Intake ◽  
Intestinal Bacteria ◽  
Average Weight ◽  
Feed Ingredients ◽  
Randomized Design ◽  
Protein Feed

This study aims to feeding of protein source feed ingredients in macroparticle or microparticles with the addition of bitter mustard root to the small intestinal bacteria population and nutrient intake in quails. The experimental livestock used by the layer phase quail were 200 birds with an average weight of 149 ± 10.88 g, protein feed ingredients in the form of soybean meal and fish meal, calcium comes from eggshells and mustard root powder as a prebiotic. The research design was a completely randomized design with 4 treatment and 5 replaice (each 10 bird). The treatment were P1= Feed with protein and calcium macroparticles; P2= Feed with protein and calcium microparticles; P3= Feed with protein and calcium macroparticles + bitter mustard root powder 1,2% dan P4= Feed with protein and calcium microparticles + bitter mustard root powder 1,2%. The parameters measured were population of  lactic acid bacteria, coliform, pH, protein and calcium intake. Data were analyzed using analysis of variance (Anova) and different test (Duncan) on 5% level. The results showed that the use of rations with protein and calcium feed ingredients in macroparticles or microparticles with the addition of bitter mustard root powder had a significant effect (p<0,05) on  population of small intestine bacteria and nutrient intake. The use of protein and calcium microparticles with the addition of 1.2% bitter mustard root powder (P4) was able to increase the population of lactic acid bacteria and nutrient intake (protein and calcium) and decreased coliform population and small intestine pH compared to other treatments (P1-P3). The conclusion is feeding of protein and calcium microparticles and addition of 1.2% bitter mustard root powder could increase the population of lactic acid bacteria, protein and calcium intake and reduce the coliform population and the pH of the small intestine.

scholarly journals Probiotic candidates among dairy Lactobacilli and Streptococcus thermophiles strains for control of the oral pathogen Porphyromonas gingivalis

Folia Medica ◽  
2021 ◽  
Vol 63 (5) ◽  
pp. 720-725
Author(s):  
Michaela Michaylova ◽  
Tsvetelina Yungareva ◽  
Zoltan Urshev ◽  
Yana Dermendzieva ◽  
Blagovesta Yaneva ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Porphyromonas Gingivalis ◽  
Antimicrobial Effect ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Inhibitory Effect ◽  
Culture Collection ◽  
Freeze Dried

Introduction: The gram-negative bacterium Porphyromonas gingivalis is a major causative agent of periodontitis in adults. It is also associated with disorders of the cardiovascular and endocrine systems, rheumatoid arthritis, pancreatic cancer, and Alzheimer&rsquo;s disease. Lactic acid bacteria (LAB) present in the oral cavity or introduced as probiotic preparations can support successful treatment of periodontitis due to their antagonism with the pathogen. Aim: The aim of this study was in vitro assessment of the antimicrobial activity of Lactobacillus spp. and Streptococcus thermophilus against P. gingivalis. Materials and methods: The antimicrobial effect of lactobacilli or S. thermophilus from the LBB Culture collection against P. gingivalis DSM 20709 was evaluated with the well diffusion assay on Wilkins Chalgren blood agar. Inhibition of the pathogen was evaluated by measuring the diameter of clear zones around the wells. Results: Application of milk fermented with selected LAB resulted in &#1072; bacteriostatic effect. The most active culture was S. thermophilus 187/4, followed by L. delbr. ssp. bulgaricus (LBB.B1054, C3/2 and LBB.B120), L. helveticus LBB.H48/1 and L. rhamnosus I-1/13. The respective reconstituted freeze-dried preparations had a stronger inhibitory effect on the pathogen with the formation of clear bactericidal zones. The effect of milk acidified with lactic acid was apparent with minimal bactericidal zone observed at concentration of 0.1%. The effectiveness of the assay was confirmed with Elgydium and Eludril. Conclusions:P. gingivalis DSM 20709 was sensitive to the metabolites produced in fermented milk by selected strains of L. delbr. ssp. bulgaricus, L. helveticus, L. rhamnosus, and S. thermophilus. Reconstituted freeze dried fermented milk had &#1072; stronger inhibitory effect compared to fresh samples. Lactic acid produced by lactic acid bacteria was the key component for inhibition of the pathogen.

scholarly journals Screening of Bacteriocinogenic Lactic Acid Bacteria and Their Characterization as Potential Probiotics

2020 ◽  
Vol 8 (3) ◽  
pp. 393 ◽  
Author(s):  
Ana Pinto ◽  
Joana Barbosa ◽  
Helena Albano ◽  
Joana Isidro ◽  
Paula Teixeira
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Foodborne Pathogens ◽  
Food Products ◽  
In Vivo Studies ◽  
Rrna Gene ◽  
Gene Sequence Analysis

Probiotics are living microorganisms used as nutritional additives that confer health benefits on the host. Their use in food products is very attractive, especially if they could also inhibit important foodborne pathogens. In this study, antimicrobial activity against several foodborne pathogens was screened for 280 lactic acid bacteria (LAB) isolated from different food products and the probiotic characteristics of bacteriocinogenic isolates were evaluated. Seven out of 280 LAB isolates were selected due to their bacteriocinogenic properties and identified by 16S rRNA gene sequence analysis as Pediococcus pentosaceus (n = 6) and Lactobacillus plantarum (n = 1). Virulence factors and antibiotic resistances were not detected for any of the isolates. Except for L. plantarum R23, all the isolates were able to survive through the simulated gastrointestinal tract conditions. Only P. pentosaceus CFF4 was able to adhere to Caco-2 cells after the simulated gastrointestinal tract passage. In conclusion, even though in vivo studies should be performed, P. pentosaceus CFF4, which was also able to inhibit the growth of foodborne pathogens in vitro, seems to be a potential probiotic to be used in the food industry.

scholarly journals Potential Rates of Fermentation in Digesta from the Gastrointestinal Tract of Pigs: Effect of Feeding Fermented Liquid Feed

2003 ◽  
Vol 69 (1) ◽  
pp. 408-418 ◽  
Author(s):  
Ole Højberg ◽  
Nuria Canibe ◽  
Bettina Knudsen ◽  
Bent Borg Jensen
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
Distal Part ◽  
Standing Stock ◽  
Short Chain ◽  
Coliform Bacteria ◽  
Liquid Feed

ABSTRACT Microbial catabolic capacity in digesta from the gastrointestinal tract of pigs fed either dry feed or fermented liquid feed (FLF) was determined with the PhenePlate multisubstrate system. The in vitro technique was modified to analyze the kinetics of substrate catabolism mediated by the standing stock of enzymes (potential rates of fermentation), allowing a quantitative evaluation of the dietary effect on the catabolic capacity of the microbiota. In total, the potential rates of fermentation were significantly reduced in digesta from the large intestine (cecum, P < 0.1; colon, P < 0.01; and rectum, P < 0.0001) of pigs fed FLF compared to pigs fed dry feed. No effect of diet was observed in the stomach (P = 0.71) or the distal part of the small intestine (P = 0.97). The highest rates of fermentation and the most significant effect of diet were observed for readily fermentable carbohydrates like maltose, sucrose, and lactose. Feeding FLF to pigs also led to a reduction in the large intestine of the total counts of anaerobic bacteria in general and lactic acid bacteria specifically, as well as of microbial activity, as determined by the concentration of ATP and short-chain fatty acids. The low-molecular-weight carbohydrates were fermented mainly to lactic acid in the FLF before being fed to the animals. This may have limited microbial nutrient availability in the digesta reaching the large intestine of pigs fed FLF and may have caused the observed reduction in activity and density of the cecal and colonic microbial population. On the other hand, feeding FLF to pigs reduced the viable counts of coliform bacteria (indicator of Escherichia coli and Salmonella spp.) most profoundly in the stomach and the distal part of the small intestine, probably due to the bactericidal effect of lactic acid and low pH. The results presented clearly demonstrate that feeding FLF to pigs had a great impact on the indigenous microbiota, as reflected in bacterial numbers, short-chain fatty acid concentration, and substrate utilization. However, completely different mechanisms may be involved in the proximal and the distal parts of the gastrointestinal tract. The present study illustrates the utility of the PhenePlate system for quantifying the catabolic capacity of the indigenous gastrointestinal tract microbiota.

scholarly journals Characterization of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Ethiopian Rhode Island Red Chicken and their Potential Use as Probiotics

2019 ◽  
Author(s):  
Kibrnesh Tegenaw Tsega ◽  
Kagira Maina ◽  
Nega Berhane
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Rhode Island ◽  
Gene Sequence ◽  
Cell Surface Hydrophobicity ◽  
Feed Additives ◽  
Good Survival ◽  
Rrna Gene

Abstract Background Chicken productivity and survival is affected by environmental stress and incidences of different diseases. After the ban of antibiotics growth promoters by different countries, the use of probiotics feed additives is mentioned as one alternative to antibiotics. The objective of this study was to isolate and identify potential probiotic lactic acid bacteria strains from the digestive tract content of Rhode Island Red chicken. Results The study was conducted in Gondar town of Amhara region, North West Ethiopia from November, 2018 to March, 2019. From the gastro intestinal content of 5 chicken 190 isolates were randomly selected. The isolates were identified and screened by their biochemical, morphological and 16S rRNA gene sequence. The in vitro probiotic potential of isolates was characterized by various tests. From 190 isolates 73 were found as gram positive, catalase negative and were able to grow at pH4. The bacteria were either rod (83.6%) or round (16.4%) shaped. Ten isolates were then randomly selected for further in vitro probiotic characterization due to higher cell surface hydrophobicity, good survival at pH2 and survival at 0.1% bile salt. From the 10 isolates 6 were able to survive at pH1 for 3hrs incubation. Five isolates (IS1, IS2, IS5, IS7 and IS8) were resistance to ampicillin, chloramphenicol, ciprofloxacin and erythromycin antibiotics. All the ten isolates showed antagonistic activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Staphylococcus intermedius and Salmonella enteritidis. The optimum temperature for all ten isolates was 450C and all the isolates were able to grow at 0.69 mol/L of NaCl, and were able to ferment galactose, glucose, fructose, mannitol, sorbitol and sucrose. Using phylogenetic analysis of 16SrRNA gene sequence; IS3 was identified as Lactobacillus salivarius while IS4, IS6 and IS7 were identified as Lactobacillus reuteri. Conclusion The study concludes that the 10 selected isolates can be able to survive the stress conditions of gastrointestinal tract and can thus be considered as potential probiotics candidates for chickens.

scholarly journals Genetic Screening of Functional Properties of Lactic Acid Bacteria in a Fermented Pearl Millet Slurry and in the Metagenome of Fermented Starchy Foods

2011 ◽  
Vol 77 (24) ◽  
pp. 8722-8734 ◽  
Author(s):  
Williams Turpin ◽  
Christèle Humblot ◽  
Jean-Pierre Guyot
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Pearl Millet ◽  
Genetic Screening ◽  
Bile Salts ◽  
Fermented Foods ◽  
Starch Metabolism ◽  
Content Type

ABSTRACTLactic acid bacteria (LAB) (n= 152) in African pearl millet slurries and in the metagenomes of amylaceous fermented foods were investigated by screening 33 genes involved in probiotic and nutritional functions. All isolates belonged to six species of the generaPediococcusandLactobacillus, andLactobacillus fermentumwas the dominant species. We screened the isolates for the abilities to survive passage through the gastrointestinal tract and to synthesize folate and riboflavin. The isolates were also testedin vitrofor their abilities to survive exposure to bile salts and to survive at pH 2. Because the ability to hydrolyze starch confers an ecological advantage on LAB that grow in starchy matrixes as well as improving the nutritional properties of the gruels, we screened for genes involved in starch metabolism. The results showed that genes with the potential ability to survive passage through the gastrointestinal tract were widely distributed among isolates and metagenomes, whereasin vitrotests showed that only a limited set of isolates, mainly those belonging toL. fermentum, could tolerate a low pH. In contrast, the wide distribution of genes associated with bile salt tolerance, in particularbsh, is consistent with the high frequency of tolerance to bile salts observed. Genetic screening revealed a potential for folate and riboflavin synthesis in both isolates and metagenomes, as well as high variability among genes related to starch metabolism. Genetic screening of isolates and metagenomes from fermented foods is thus a promising approach for assessing the functional potential of food microbiotas.

Studies on the mode of action of probiotics: effects of the spore-specific dipicolinic acid on selected intestinal bacteria

2005 ◽  
Vol 143 (6) ◽  
pp. 529-535 ◽  
Author(s):  
A. JADAMUS ◽  
W. VAHJEN ◽  
O. SIMON
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Dipicolinic Acid ◽  
Intestinal Bacteria ◽  
E Coli ◽  
Growth Inhibiting

Bacterial growth inhibiting effects of dipicolinic acid (DPA), which occurs in spores of probiotic Bacillus spp. strains, was studied with a range of Lactobacillus spp. (13), Enterococcus spp. (14), and E. coli strains (8) in vitro. In vivo effects were studied in a broiler chicken nutrition trial and DPA supplemented feed (10 g/kg).In vitro studies showed that DPA inhibited growth of most Lactobacillus spp. strains to a larger extent than E. coli strains. Enterococci were generally influenced less by DPA. However, in each group of bacteria some strains were less inhibited by DPA than others. Intestinal lactic acid bacteria in the jejunum and ileum showed a trend (P[les ]0·1) for lower counts in birds fed the DPA-supplemented feed on day 14 and 21. Counts of enterobacteria were not significantly influenced by DPA. No differences in colony counts were observed in 28-day-old animals. Digesta pH was not statistically reduced, but a lower crop pH was recorded throughout the nutrition trial.The study showed that dipicolinic acid, present in probiotic Bacillus spp. spores, is inhibitory for in vitro growth of most lactobacilli and enterobacteria, while growth of enterococci was less inhibited. Intestinal colony counts in young broiler chickens fed a DPA-supplemented diet in part mirrored in vitro results.

Sign in / Sign up

Export Citation Format

Share Document