Therapeutic antigout and antioxidant activity of Piper betle L. in gout-induced broilers

Purpose: To determine the antioxidant and anticancer effects of Piper betle (P. betle) leaf extract on human breast cancer MCF-7 cells, and to develop transdermal patches containing the extract. Methods: The leaf extract of P. betle was prepared by maceration method, and its antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Cytotoxicity and suppression of cell migration (indices of anticancer activity) were also assessed in MCF-7 cells by sulforhodamine B (SRB) and wound healing assays, respectively. Transdermal patches were developed using the casting method, and the resultant patches were evaluated with regard to their physical appearance and mechanical properties before and after a stability test. Results: The extract exhibited antioxidant activity with half-maximal inhibitory concentration (IC50) of 30.0 ± 0.1 µg/mL. It also showed cytotoxicity with an IC50 of 114.3 ± 14.9 µg/mL, and significantly suppressed the migration of MCF-7 cells at a dose of 25 µg/mL. Based on desirable characteristics, patch base formulations containing 4.2 % pectin, 0.4 % hydroxyl propyl methylcellulose (HPMC), 0.4 % polyvinyl pyrrolidine K-90 (PVP-K90) and 3 % propylene glycol (PG) were selected for incorporation into the extract. Conclusion: Leaf extract of P. betle exhibits potential anti-breast cancer properties. A transdermal patch containing 0.03 % of the extract can be successfully developed for treatment of breast cancer.

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Uji Aktivitas Antioksidan Kombinasi Infusa Daun Sirih (Piper betle L), Ekstrak Etanolik Tanaman Bundung (Actinuscirpus grossus) dan Kulit Jeruk Nipis (Citrus aurantifolia)

Jurnal Surya Medika ◽

10.33084/jsm.v7i1.2647 ◽

2021 ◽

Vol 7 (1) ◽

pp. 115-122

Author(s):

Kunti Nastiti ◽

Noval Noval ◽

Darini Kurniawati

Keyword(s):

Antioxidant Activity ◽

Antimicrobial Properties ◽

Ethanolic Extract ◽

Chemical Compounds ◽

Positive Control ◽

Piper Betle ◽

Citrus Aurantifolia ◽

Water Solvent ◽

Dpph Method ◽

Antioxidant Effects

Piper betle L., the leaf, is the most often used for treatment and has been scientifically proven to have antibacterial activity. Actinuscirpus grossus plants are commonly found in Kalimantan and have antimicrobial properties. Citrus aurantifolia peel has antioxidant effects and nutritious chemical compounds in medicine. The purpose of this study was to determine the effect of antioxidant activity in a combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel. The research method was experimental by making Piper betle L. leaves infused with water solvent. Simplisia powder of Actinuscirpus grossus plants and Citrus aurantifolia peel extracted by maceration with 95% ethanol solvent. The combination of the three plants was then tested for antioxidants by the DPPH method. Quercetin is used as a positive control. The results showed that the combination of the three plants had moderate antioxidant activity (IC50 128 ppm), but the antioxidant activity was smaller than quercetin (IC50 16.88 ppm). The combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel has a moderate antioxidant category.

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Evaluation of bioactivity of Annona muricata, Piper betle and Mentha spicata

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016611 ◽

2017 ◽

Vol 6 (1) ◽

pp. 1

Author(s):

Antony V. Samrot ◽

Sherly H. ◽

Sahiti K. ◽

Bennet Rohan D. ◽

Raji P.

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Tamil Nadu ◽

Antioxidant Activities ◽

Biological Activities ◽

Piper Betle ◽

Annona Muricata ◽

Mentha Spicata ◽

Phytochemical Compounds ◽

Medicinal Properties

Plants produce wide variety of phytochemical compounds which have been known to perform most biological activities such as antimicrobial, anticancer, antioxidant activities etc. Plants which possess these medicinal properties are known to be medicinal plants. In this study, Annona muricata, Piper betle and Mentha spicata were collected from Chennai, Tamil Nadu, India. The crude extracts were taken using various solvents like acetone, ethanol, chloroform, toluene and petroleum ether. All the extracts were subjected for qualitative analysis for the phytochemicals. All the samples were also subjected for antibacterial activity and TLC bioautography analysis for antioxidants. Since they found to possess antioxidant activity, quantitative assay for antioxidant activity like DPPH and FRAP assays were performed.Keywords: Annona muricata, Piper betle and Mentha spicata

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IN VITRO STUDY ON ANTIOXIDANT ACTIVITY OF METHANOLIC LEAF EXTRACT OF PIPER BETLE LINN

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2018/646 ◽

2018 ◽

Vol 7 (24) ◽

pp. 2865-2869

Author(s):

Kamath Bindhu R ◽

Sabeena K

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

In Vitro Study ◽

Vitro Study ◽

Piper Betle

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Biological synthesis of iron oxide nanoparticles and evaluation of its antibacterial and antioxidant property

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10ispl1.1711 ◽

2019 ◽

Vol 10 (SPL1) ◽

Author(s):

Nurul Ain Shafina Mohd Hasni ◽

Shahnaz Majeed

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Leaf Extract ◽

Piper Betle ◽

Oxide Nanoparticles ◽

Dpph Assay ◽

Vibrio Cholera ◽

Potential Analysis ◽

Vis Spectroscopy ◽

Zeta Potential Analysis

Iron oxide nanoparticles (IONPs) have become a great interest among the researcher as it has a broad range of advantageous application in the pharmaceutical industries as well as healthcare settings. The present study was focused on the synthesis of IONPs from the aqueous extract of Piper betle leaves belongs to the genus Piper of the family Piperaceae and to evaluate the antibacterial activity against various pathogens. IONPs were synthesized from Piper betle leaf extract using 3mM of iron (III) chloride (FeCl3). The characterization of IONPs was done through UV-Vis spectroscopy, TEM, FTIR, and Zeta potential analysis. Then, the synthesized IONPs were tested for antibacterial activity against five different pathogens, which are Staphylococcus aureus, Staphylococcus epidermidis, Salmonella typhi, Escherichia coli, and Vibrio Cholera. The synergistic effect of IONPs were evaluated with ceftriaxone, gentamicin and amoxicillin. Furthermore antioxidant activity was determined using DPPH assay. The maximum absorption peak of IONPs was found at 320 nm through UV-Vis spectroscopy. The synthesized IONPs were spherical in shape with size ranges from 10 - 15 nm through TEM analysis. From FTIR spectroscopy, significant peaks were observed and showed the presence of a hydroxyl group, amine, alkane, amide, and nitrile groups, respectively. Zeta potential analysis showed IONPs were stable. The synthesized IONPs showed significant antibacterial activity against all the selected pathogens. The maximum zone of inhibition was observed on Vibrio cholera increased as the concentration of the IONPS increased. The IONPs exhibited a good synergistic effect with ceftriaxone as compared to gentamicin and amoxicillin. The IONPs showed good antioxidant properties through DPPH assay at 40 µg/ml. The IONPs were successfully synthesized from Piper betle leaf extract, which demonstrated significant antibacterial activity against various bacterial pathogens and also exhibited good antioxidant activity.

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Optimasi Masker Gel Peel-Off Ekstrak Etanolik Daun Sirih (Piper Betle L.) dengan Kombinasi Carbomer dan Polivinil Alkohol

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v17i2.11976 ◽

2020 ◽

Vol 17 (2) ◽

pp. 174-183

Author(s):

Gunawan Setiyadi ◽

Annisa Qonitah

Keyword(s):

Antioxidant Activity ◽

Physical Properties ◽

Measurement Data ◽

Piper Betle ◽

Drying Time ◽

Design Expert ◽

Betel Leaf ◽

Lattice Design ◽

Ic50 Value ◽

Optimum Formula

Betel leaf (Piper betle L.) is a plant that has potential as a source of natural antioxidants. The purpose of this study was to determine the effect of the combination of carbomer and PVA on the homogeneity, dispersibility, adhesion, viscosity, dry time, elasticity and pH of the gel peel-off mask preparation; obtain the optimum formula and determine the antioxidant activity of the optimum formula. Extraction of betel leaf was carried out using the maceration method with 96% ethanol as a solvent. The experimental design was made using Design Expert software version 11 with the Simplex Lattice Design (SLD) method with the optimized components being carbomer and PVA. The response of physical properties in the form of spreadability, adhesion, pH, viscosity, dry time, and elasticity of each formula is measured and the measurement data is processed with Design Expert software to determine the optimum formula based on predetermined criteria. The verification dosage form is made based on the optimum formula composition produced. The response of the optimum physical properties of the prediction formula was compared with the results of the verification preparation with the One sample t-test. The yield of thick betel leaf extract in this study was 20.26%. The optimum formula has homogeneous characteristics with a distinctive odor of dark green betel leaf and semi-solid form, spreadability 49.25 ± 0.29 cm2, adhesion 4.77 ± 0.22 seconds, pH 7.26 ± 0.119, viscosity 763 ± 12.47 dPas, drying time 29.68 ± 0.52 minutes, elasticity 1.05 ± 0.04%. The antioxidant activity of betel leaf ethanolic extract has an IC50 value of 7.62 µg / mL and the optimum formula has an IC50 value of 111.25 µg / mL.

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Antioxidant Activity, Phenolic, Flavonoid and Tannin Content of Piper Betle and Leucosyke Capitella

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v8n1.115 ◽

2014 ◽

Vol 8 (1) ◽

Author(s):

Murni Sundang ◽

Sharifah Norfarahani Syed Nasir ◽

Coswald Stephen Sipaut ◽

Hasnah Othman

Keyword(s):

Antioxidant Activity ◽

Vitamin E ◽

Petroleum Ether ◽

Butylated Hydroxytoluene ◽

Total Phenolic ◽

Piper Betle ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Tannin Content ◽

Tannin Extract

Piper betle and Leucosyke capitella are plants that are commonly used as traditional medicine. In this study, antioxidant Activity, Phenolic, Flavonoids and Tannin content of these plants were evaluated. The plants were extracted using petroleum ether, acetone and methanol. The total phenolic, flavonoid and tannin extract of both plants is in the order of acetone > methanol > petroleum ether. The result shows that the total phenolic, flavonoid content for Piper Betle and Leucosyke Capitella is in the range of 31.25 to 47.48 mg/g and 1.68 to 7.19 mg/g, respectively. For Piper betle, the total flavonoid content is in the range of 29.58 to 46.08 mg/g and for Leucosyke capitella is in the range of 1.08 to 6.83 mg/g. It is also found that Piper betle extracted with methanol has higher antioxidant activity than vitamin E, butylated hydroxytoluene (BHT) and catechin but lower than quercetin.

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ANTIOXIDANT ACTIVITY (PHENOL AND FLAVONOID CONTENT ) OF THREE DIFFERENT CULTIVARS OF PIPER BETLE L. (PIPERACEAE)

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v8i5-s.1978 ◽

2018 ◽

Vol 8 (5-s) ◽

pp. 143-148

Author(s):

SAPARA SEKHAR HARINI ◽

Pagadala R Sougandhi ◽

DR SHOBHA RANI TENKAYALA ◽

Kamalamma Ramalingam Gopinath

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Piper Betle ◽

Drug Preparation ◽

Phenol Content ◽

Flavonoid Content ◽

Antioxidant Power ◽

Dpph Method

In the present study, an attempt has been made for the estimation of total phenol and flavonoid content and their radical scavenging properties using Piper betle (L.) leaves. In that, Cultivars i.e., Nov Bangla (NB), Sirugamani-1 (SGM-1) and Halisar Sanchi (HS) were selected for this study. The total phenolic content was ranged from 95.04 to 127.33 mg/100g equivalent to gallic acid and flavonoids were ranged from 51.72 to 61.08 mg/ 100g equivalent to standards of Catechin. In vitro antioxidant activity was estimated using 1,1-diphenyl-2-picryl hydrazyl (DPPH), free radical scavenging activity, improved ABTS radical cation decolorization assay and ferric reducing antioxidant power (FRAP) assay. Among all the cultivars, The highest Phenol content (93.79%) was observed for Sirugamani-1 by DPPH method and highest Phenol content (96.12% & 6791.86 (µg/g) was obtained for Halisar Sanchi by ABTS assay and FRAP activity respectively. The study revealed that the leaves of Piper betle (L.) has higher amount of antioxidant activity and it could be used for any novel drug preparation.

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