Electrospun herbal nanofibrous wound dressings for skin tissue engineering

Skin is a hierarchical and multi-cellular organ exposed to the external environment with a key protective and regulatory role. Wounds caused by disease and trauma can lead to a loss of function, which can be debilitating and even cause death. Accelerating the natural skin healing process and minimizing the risk of infection is a clinical challenge. Electrospinning is a key technology in the development of wound dressings and skin substitutes as it enables extracellular matrix-mimicking fibrous structures and delivery of bioactive materials. Honey is a promising biomaterial for use in skin tissue engineering applications and has antimicrobial properties and potential tissue regenerative properties. This preliminary study investigates a solution electrospun composite nanofibrous mesh based on polycaprolactone and a medical grade honey, SurgihoneyRO. The processing conditions were optimized and assessed by scanning electron microscopy to fabricate meshes with uniform fiber diameters and minimal presence of beads. The chemistry of the composite meshes was examined using Fourier transform infrared spectroscopy and X-ray photon spectroscopy showing incorporation of honey into the polymer matrix. Meshes incorporating honey had lower mechanical properties due to lower polymer content but were more hydrophilic, resulting in an increase in swelling and an accelerated degradation profile. The biocompatibility of the meshes was assessed using human dermal fibroblasts and adipose-derived stem cells, which showed comparable or higher cell metabolic activity and viability for SurgihoneyRO-containing meshes compared to polycaprolactone only meshes. The meshes showed no antibacterial properties in a disk diffusion test due to a lack of hydrogen peroxide production and release. The developed polycaprolactone-honey nanofibrous meshes have potential for use in skin applications.

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Graphene oxide-modified electrospun polyvinyl alcohol nanofibrous scaffolds with potential as skin wound dressings

RSC Advances ◽

10.1039/c7ra03997b ◽

2017 ◽

Vol 7 (46) ◽

pp. 28826-28836 ◽

Cited By ~ 19

Author(s):

Qiang Zhang ◽

Qiaoyue Du ◽

Yanan Zhao ◽

Feixiang Chen ◽

Zijian Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Polyvinyl Alcohol ◽

Potential Application ◽

Skin Wound ◽

Wound Dressings ◽

Skin Tissue ◽

Skin Tissue Engineering ◽

Nanofibrous Scaffolds ◽

Good Biocompatibility

Graphene oxide-modified electrospun polyvinyl alcohol nanofibrous scaffolds exhibit good biocompatibility and have potential application in skin tissue engineering.

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Polydopamine modification of silk fibroin membranes significantly promotes their wound healing effect

Biomaterials Science ◽

10.1039/c9bm00974d ◽

2019 ◽

Vol 7 (12) ◽

pp. 5232-5237 ◽

Cited By ~ 7

Author(s):

Ying Zhang ◽

Leihao Lu ◽

Yuping Chen ◽

Jie Wang ◽

Yuyin Chen ◽

...

Keyword(s):

Tissue Engineering ◽

Wound Healing ◽

Silk Fibroin ◽

Wound Dressings ◽

Skin Tissue ◽

Natural Polymer ◽

Skin Tissue Engineering ◽

Healing Effect ◽

Wound Healing Effect

Natural polymer-based wound dressings have gained great attention in skin tissue engineering.

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Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

10.20944/preprints201812.0170.v1 ◽

2018 ◽

Cited By ~ 1

Author(s):

Lucie Bacakova ◽

Julia Pajorova ◽

Marketa Bacakova ◽

Anne Skogberg ◽

Pasi Kallio ◽

...

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Wound Healing ◽

Contact Lenses ◽

Heart Defects ◽

Cellulose Nanofibers ◽

Wound Dressings ◽

Skin Tissue ◽

Skin Tissue Engineering ◽

Wide Range

Nanocellulose is cellulose in the form of nanostructures, i.e. features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, e.g. in bacterial cellulose; nanofibers, e.g. in electrospun matrices; nanowhiskers and nanocrystals. These structures can be further assembled into bigger 2D and 3D nano-, micro- and macro-structures, such as nanoplatelets, membranes, films, microparticles and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluonacetobacter), plants (trees, shrubs, herbs), algae (Cladophora) and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology and biomedical applications, e.g. for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

Nanomaterials ◽

10.3390/nano9020164 ◽

2019 ◽

Vol 9 (2) ◽

pp. 164 ◽

Cited By ~ 55

Author(s):

Lucie Bacakova ◽

Julia Pajorova ◽

Marketa Bacakova ◽

Anne Skogberg ◽

Pasi Kallio ◽

...

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Wound Healing ◽

Contact Lenses ◽

Heart Defects ◽

Cellulose Nanofibers ◽

Wound Dressings ◽

Skin Tissue ◽

Skin Tissue Engineering ◽

Wide Range

Nanocellulose is cellulose in the form of nanostructures, i.e., features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, found in bacterial cellulose; nanofibers, present particularly in electrospun matrices; and nanowhiskers, nanocrystals, nanorods, and nanoballs. These structures can be further assembled into bigger two-dimensional (2D) and three-dimensional (3D) nano-, micro-, and macro-structures, such as nanoplatelets, membranes, films, microparticles, and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluconacetobacter), plants (trees, shrubs, herbs), algae (Cladophora), and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology, and biomedical applications, namely for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

10.31224/osf.io/xs64k ◽

2018 ◽

Cited By ~ 1

Author(s):

Antonios Keirouz ◽

Giuseppino Fortunato ◽

Anthony Callanan ◽

Norbert Radacsi

Keyword(s):

Tissue Engineering ◽

Tensile Modulus ◽

Dermal Fibroblasts ◽

Skin Tissue ◽

Skin Substitute ◽

Electrospinning Technique ◽

Skin Tissue Engineering ◽

Needleless Electrospinning ◽

High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

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