Timing of cold temperature exposure affects root and shoot frost hardiness ofPicea Marianacontainer seedlings

AbstractHalophilic Archaea are known to tolerate multiple extreme conditions on Earth and have been proposed as models for astrobiology. In order to assess the importance of cold-adaptation of these microorganisms in surviving stratospheric conditions, we launched live, liquid cultures of two species, the mesophilic model Halobacterium sp. NRC-1 and the cold-adapted Antarctic isolate Halorubrum lacusprofundi ATCC 49239, on helium balloons. After return to Earth, the cold-adapted species showed nearly complete survival while the mesophilic species exhibited slightly reduced viability. Parallel studies found that the cold-adapted species was also better able to survive freezing and thawing in the laboratory. Genome-wide transcriptomic analysis was used to compare the two haloarchaea at optimum growth temperatures versus low temperatures supporting growth. The cold-adapted species displayed perturbation of a majority of genes upon cold temperature exposure, divided evenly between up-regulated and down-regulated genes, while the mesophile exhibited perturbation of only a fifth of its genes, with nearly two-thirds being down-regulated. These results underscore the importance of genetic responses of H. lacusprofundi to cold temperature for enhanced survival in the stratosphere.

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Influence of Cyclic Cold Temperature Stress on Watermelon Seedling Growth, Earliness, Yield, and Quality

HortScience ◽

10.21273/hortsci.33.3.546d ◽

1998 ◽

Vol 33 (3) ◽

pp. 546d-546

Author(s):

Ahmet Korkmaz ◽

Robert J. Dufault

Keyword(s):

South Carolina ◽

Cold Stress ◽

Seedling Growth ◽

Cold Temperature ◽

Yield Potential ◽

Cold Temperatures ◽

Yield And Quality ◽

Long Term Effect ◽

Total Productivity ◽

Temperature Exposure

Watermelon seedlings may be repeatedly exposed to temperatures alternating between almost freezing and optimal temperatures for growth during stand establishment in coastal South Carolina. `Carnival' watermelon transplants were exposed to 2 °C for 3, 6, and 9 h, for 1, 3, 6, and 9 days in a walk-in cooler and then to warm temperatures (24 °C) immediately prior to field planting. Our objective was to determine the long term effect of early season cold temperature exposure on seedling growth, earliness, yield and quality by simulating the cold/warm alternations possible in the field. Cold-stressed transplants were field planted after the risk of ambient cold stress was negligible. Exposure to cycling cold temperatures generally did not affect earliness, total productivity and quality, although seedling growth characteristics were reduced in response to the longer cold-stress treatment. Therefore, cold temperature stresses occurring in the field at transplanting have negligible beneficial or detrimental effects on yield potential of `Carnival' watermelon.

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Self-reported effects of cold temperature exposure in persons with tetraplegia

Journal of Spinal Cord Medicine ◽

10.1080/10790268.2016.1154670 ◽

2016 ◽

Vol 40 (4) ◽

pp. 389-395 ◽

Cited By ~ 3

Author(s):

John P. Handrakis ◽

Dwindally Rosado-Rivera ◽

Kamaldeep Singh ◽

Kirsten Swonger ◽

Frank Azarelo ◽

...

Keyword(s):

Cold Temperature ◽

Temperature Exposure

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Effect of cold-temperature exposure and acclimation on amino acid pool changes and enzyme activities of rat brown adipose tissue

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/0304-4165(87)90191-7 ◽

1987 ◽

Vol 925 (3) ◽

pp. 265-271 ◽

Cited By ~ 15

Author(s):

F.J. López-Soriano ◽

M. Alemany

Keyword(s):

Adipose Tissue ◽

Amino Acid ◽

Brown Adipose Tissue ◽

Enzyme Activities ◽

Cold Temperature ◽

Amino Acid Pool ◽

Acid Pool ◽

Brown Adipose ◽

Temperature Exposure

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Evidence for pre-climacteric activation of AOX transcription during cold-induced conditioning to ripen in European pear (Pyrus communis L.)

10.1101/755686 ◽

2019 ◽

Cited By ~ 1

Author(s):

Christopher Hendrickson ◽

Seanna Hewitt ◽

Mark E. Swanson ◽

Todd Einhorn ◽

Amit Dhingra

Keyword(s):

Time Course ◽

Transcript Abundance ◽

Ethylene Biosynthesis ◽

Pyrus Communis ◽

Cold Temperature ◽

Fruit Peel ◽

Physiological Indicators ◽

Conditioning Model ◽

Time Course Experiment ◽

Temperature Exposure

AbstractEuropean pears (Pyrus communis L.) require a range of cold-temperature exposure to induce ethylene biosynthesis and fruit ripening. Physiological and hormonal responses to cold temperature storage in pear have been well characterized, but the molecular underpinnings of these phenomena remain unclear. An established low-temperature conditioning model was used to induce ripening of ‘D’Anjou’ and ‘Bartlett’ pear cultivars and quantify the expression of key genes representing ripening-related metabolic pathways in comparison to non-conditioned fruit. Physiological indicators of pear ripening were recorded, and fruit peel tissue sampled in parallel, during the cold-conditioning and ripening time-course experiment to correlate gene expression to ontogeny. Two complementary approaches, Nonparametric Multi-Dimensional Scaling and efficiency-corrected 2-(ΔΔCt), were used to identify genes exhibiting the most variability in expression. Interestingly, the enhanced alternative oxidase (AOX) transcript abundance at the pre-climacteric stage in ‘Bartlett’ and ‘D’Anjou’ at the peak of the conditioning treatments suggests that AOX may play a key and a novel role in the achievement of ripening competency. There were indications that cold-sensing and signaling elements from ABA and auxin pathways modulate the S1-S2 ethylene transition in European pears, and that the S1-S2 ethylene biosynthesis transition is more pronounced in ‘Bartlett’ as compared to ‘D’Anjou’ pear. This information has implications in preventing post-harvest losses of this important crop.

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Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

10.1101/851733 ◽

2019 ◽

Cited By ~ 2

Author(s):

Seanna Hewitt ◽

Christopher A. Hendrickson ◽

Amit Dhingra

Keyword(s):

Time Course ◽

Molecular Mechanisms ◽

Developmental Stages ◽

Global Gene Expression ◽

Ethylene Biosynthesis ◽

Cold Temperature ◽

European Pear ◽

System 2 ◽

Autocatalytic System ◽

Temperature Exposure

AbstractEuropean pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The physiological responses of pear to cold-temperature-induced ripening have been well characterized, but the molecular mechanisms underlying this phenomenon continue to be elucidated. This study employed established cold temperature conditioning treatments for ripening of two pear cultivars, ‘D’Anjou’ and ‘Bartlett’. Using a time-course transcriptomics approach, global gene expression responses of each cultivar were assessed at four different developmental stages during the cold conditioning process. Differential expression, functional annotation, and gene ontology enrichment analyses were performed. Interestingly, evidence for the involvement of cold-induced, vernalization-related genes and repressors of endodormancy release was found. These genes have not previously been described to play a role in fruit during the ripening transition. The resulting data provide insight into cultivar-specific mechanisms of cold-induced transcriptional regulation of ripening in European pear, as well as a unique comparative analysis of the two cultivars with very different cold conditioning requirements.

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