PALYNOLOGICAL INTERPRETATION OF GAMMA RAY AND COLCHICINE INDUCED MUTATION IN CHRYSANTHEMUM CULTIVARS

1998 ◽  
Vol 46 (3) ◽  
pp. 199-207 ◽  
Author(s):  
Kalyani Datta ◽  
Subodh Datta K.
Keyword(s):  
Pollen Morphology ◽  
Gamma Ray ◽  
Pollen Grains ◽  
Flower Color ◽  
Colchicine Treatment ◽  
Pollen Grain ◽  
Surface Pattern ◽  
Different Types ◽  
Chromosomal Status ◽  
Appreciable Variation

Palynological investigation on five cultivars of Chrysanthemun morifolium Ramat. and their induced somatic flower color mutants obtained through gamma irradiation and colchicine treatment has been carried out using LM and SEM. The objective of the work was to analyze the changes in the pollen morphology which occurred in mutagen-induced somatic flower color mutants and to verify if there is any correlation between the changes in flower color/type and pollen morphology. Chromosomal status of all the cultivars and their somatic mutants is hexaploid (6 n = 54), but different types of chromosomal aberrations have been observed. Significant increase in pollen grain sterility was found in all the mutants. The pollen grains of all the cultivars and their mutants are basically 3(-4) zonocolporate with tectate spinose exine having perforations. No appreciable variation in pollen apertural character was noticed in any of the mutants. Significant changes in pollen exine surface pattern were found in 4 mutant varieties. The changes are inconsistent and do not correspond to the intensity of radiation.

scholarly journals Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

2017 ◽  
Vol 24 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ebadi-Nahari Mostafa ◽  
Nikzat-Siahkolaee Sedigheh ◽  
Eftekharian Rosa
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Type ◽  
Upgma Tree ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
The Family ◽  
The World ◽  
Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

scholarly journals Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

2013 ◽  
Vol 85 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Saulo M. Sousa ◽  
Patrícia M.O. Pierre ◽  
Giovana A. Torres ◽  
Lisete C. Davide ◽  
Lyderson F. Viccini
Keyword(s):  
Chromosome Numbers ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Positive Association ◽  
Single Species ◽  
Morphological Data ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Exine Ornamentation ◽  
Different Types

The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri- and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia may be used as an additional taxonomic character of the genus.

Pollen morphology of the Rosaceae of western Canada. IV. Luetkea, Oemleria, Physocarpus, Prunus

1991 ◽  
Vol 69 (12) ◽  
pp. 2583-2596 ◽  
Author(s):  
R. J. Hebda ◽  
C. C. Chinnappa ◽  
B. M. Smith
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Western Canada ◽  
Prunus Virginiana ◽  
Prunus Spinosa ◽  
Pore Area ◽  
Pollen Grain Size ◽  
Triple Points ◽  
Scanning Electron

Pollen grains of 12 species of western Canadian rosaceous genera, Luetkea, Oemleria, Physocarpus, and Prunus, were studied in the light microscope and scanning electron microscope. All pollen grains are produced as isopolar, radially symmetrical, usually tricolporate monads. Grains are predominantly spheroidal with a circular to triangular amb. Most of the species exhibit a well-developed chambered aperture complex at the equator, formed by a pair of sexinal flaps whereas in Prunus spinosa, Prunus americana, and Prunus virginiana the chamber is absent or weakly developed. Oemleria cerasiformis has no pore flaps. Apertures of Prunus emarginata and Prunus virginiana often have an equatorial bridge over the aperture complex. The exine of Prunus, Physocarpus, and Luetkea pollen is tectate perforate, like that of many Rosaceae. Sculpturing consists of ridges and valleys that form a striate to rugulate pattern. Ridges and valleys tend to be mainly parallel to the colpus but often loop near the poles. Pollen grains of Prunus spinosa are always rugulate or vermiculate with ridges arranged randomly. Oemleria cerasiformis pollen appears tectate imperforate. Ridges and valleys intersect at triple points in the subpolar region and curve in a semicircle around the pore area. On this basis Oemleria pollen is distinct from all other western Canadian Rosaceae. Pollen grain size ranges from small for Luetkea pectinata (17 μm in diameter) to medium for Prunus spinosa (36 × 33 μm). Key words: pollen morphology, Rosaceae, Western Canada, systematics.

Morphological studies of pollen grains of the Polish endemic species of the genus Rubus (Rosaceae)

Biologia ◽  
2012 ◽  
Vol 67 (1) ◽  
Author(s):  
Dorota Wrońska-Pilarek ◽  
Andrzej Jagodziński ◽  
Tomasz Maliński
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Study Objective ◽  
Mean Values ◽  
Morphological Studies ◽  
Exine Sculpture ◽  
Pollen Grain Morphology

AbstractThe pollen of six taxa of the genus Rubus endemic to Poland (R. capitulatus, R. chaerophylloides, R. ostroviensis, R. posnaniensis, R. seebergensis and R. spribillei)was studied using light and scanning electron microscopy. The study objective was to extend the knowledge of the species pollen morphology and to investigate whether pollen morphology may help to taxonomically distinguish a very rare bramble species. Study material was derived from six natural localities where the species occur in the Wielkopolska region (Poland). Ten quantitative pollen grain features were analysed as well as the following qualitative ones: outline, shape, type of bridge and exine sculpture. Only mature, correctly formed pollen grains (30 per specimen) were measured and 180 pollen grains were examined in total. It was found that R. capitulatus and R. seebergensis had the highest mean values of P, E and Le features. In addition, pollen grains of R. capitulatus were most flattened (mean P/E ratio equals 1.14), while those of R. seebergensis were most elongated (mean P/E ratio equals 1.27). R. spribillei was the only species with striae of similar width as muri, whereas striae in the remaining species were wider. R. chaerophylloides and R. posnaniensis were characterised by specific, similar exine sculpture. Nevertheless, it should be stated that differences between pollen features of the species studied are so small and the variability of these features are so large that it is difficult to clearly identify the species studied. Therefore, pollen grain morphology can serve only as an auxiliary feature for the determination of these species.

scholarly journals Pollen Morphology of Some Species of the Genus Sansevieria Petagna (Asparagaceae)

2017 ◽  
Vol 59 (2) ◽  
pp. 63-75 ◽  
Author(s):  
Małgorzata Klimko ◽  
Renata Nowińska ◽  
Paul Wilkin ◽  
Justyna Wiland-Szymańska
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Prolate Spheroidal ◽  
Pollen Grain Morphology ◽  
First Time ◽  
Scanning Electron ◽  
Eleven Species

AbstractPollen grains of 15 species of the genus Sansevieria were examined using light and scanning electron microscopy. The pollen grains were single, but dyads were also found. The basic shape of the pollen grains (with chemical treatment) was subprolate, prolate and prolate-spheroidal and the pollen grains were large (> 50 mm). The pollen grains observed in SEM were prolate-spheroidal, subprolate, prolate and perprolate. The aperture occupied nearly the entire distal hemisphere. The surface of non-apertural areas was microreticulate, psilate-perforate, fossulate and verrucate. The surface of the apertural area was psilate-perforate-verrucate, microreticulate-verrucategemmate and psilate-perforate. The tectum was homogeneous, with perforations. Our results suggest that some species cannot be separated based on pollen grain morphology, but only may be categorized into their groups. The present study investigated pollen grain morphology in eleven species for the first time in terms of pollen micromorphology and provided some important new data.

scholarly journals Pollen morphology of Polish native species of the Rosa genus (Rosaceae) and its relation to systematics

2011 ◽  
Vol 80 (3) ◽  
pp. 221-232 ◽  
Author(s):  
Dorota Wrońska-Pilarek
Keyword(s):  
Native Species ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Morphological Features ◽  
Diagnostic Features ◽  
Qualitative Traits ◽  
Outline Shape ◽  
Analytical Results ◽  
Exine Sculpturing

The morphology of pollen grains of 16 species from the <em>Rosa </em>L. genus were studied (i.e. <em>R. agrestis</em>, <em>R. canina</em>, <em>R. dumalis</em>, <em>R. gallica</em>, <em>R. inodora</em>, <em>R. jundzillii</em>, <em>R. kostrakiewiczii</em>, <em>R. majalis</em>, <em>R. micrantha</em>, <em>R. mollis</em>, <em>R. pendulina</em>, <em>R. rubiginosa</em>, <em>R. sherardii</em>, <em>R. tomentosa</em>, <em>R. villosa</em>, and <em>R. zalana</em>). The material came from 16 native localities of those species in Poland. The measurements are based on at least 30-50 randomly selected, fully developed pollen grains per specimen. In total, 500 pollen grains were examined. They were analysed for 13 quantitative features of pollen grains and exine sculpturing and the following qualitative traits: outline, shape, "operculum" structure. The diagnostic features of pollen grains of studied species were: length of polar and equatorial axes and length of ectocolpi. The above-mentioned pollen grain morphological features make isolation of one species possible: <em>R. gallica</em>. <em>R. gallica </em>is distinguished for its highest values of the length of polar and equatorial axes, and the length of ectocolpi. The obtained analytical results of operculum and exine sculpture features, considered as diagnostic, corroborated only slightly their priority significance for the isolation of the examined species and sections. The collected data failed to confirm fully the current taxonomical division of the <em>Rosa </em>genus into sections (only section <em>Gallicanae</em> from <em>R. gallica </em>is isolated) as well as consanguinity relationships between the examined species from the <em>Caninae </em>section. On the dendrogram, both species closely related with each other as well as those from other developmental lines were found in the same group. These equivocal results are by no means surprising because the <em>Caninae </em>section is the most polymorphic group in the <em>Rosa </em>genus, and contemporary <em>Caninae </em>are of the nature of a swarm of <em>R. canina </em>hybrids as a link combining all taxons of the section.

scholarly journals SCANNING ELECTRON MICROSCOPY STUDIES ON PLECTRANTHUS- AN IMPORTANT BEE FORAGE PLANT OF HIMACHAL PRADESH, INDIA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Radhika Jamwal
Keyword(s):  
Electron Microscopy ◽  
Pollen Grains ◽  
Himachal Pradesh ◽  
Pollen Grain ◽  
Surface Pattern ◽  
Climatic Zones ◽  
Forage Plants ◽  
Shape Class ◽  
Symmetry Surface ◽  
Scanning Electron

The present investigations were conducted on two important nectar and pollen yielding plants viz. Plecranthus coesta and Plecranthus rugosus from different agro-climatic zones of Himachal Pradesh. The bee forage plants were collected, identified, classified and diffentiating characters of two species were noted. Apart from this, pollen grains of P. coesta and P. rugosus were also studied using light and scanning electron microscope. The pollen grains were observed in terms of aggregation, shape, shape class, size, aperture, polarity, symmetry, surface pattern/exine complexity. Both P. coesta and P. rugosus had solitary medium sized, hexacolpate, isopolar and radially symmetric grains. However, the pollen grain of two species varies in shape and exine complexity. P. coesta pollens were round/ circular and were prolate, whereas in P. rugosus pollens were round/oval and subprolate-prolate. Variation was also found in the exine complexity of the two species. Exine was tectate, tectum was nearly reticulate / scabrate in P. coesta. But in P. rugosus and exine was either tectate or semitectate, tectum was reticulate, microreticulate.

scholarly journals Pollen morphology of Plantago species native to Poland and their taxonomic implications

2011 ◽  
Vol 73 (4) ◽  
pp. 315-325 ◽  
Author(s):  
Małgorzata Klimko ◽  
Krystyna Idzikowska ◽  
Mariola Truchan ◽  
Anna Kreft
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Pollen Morphology ◽  
Pore Diameter ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Prolate Spheroidal ◽  
Taxonomic Implications ◽  
Scanning Electron

Pollen grains of 9 species of the genus <em>Plantago</em> (Plantaginaceae), including 8 taxa native to Poland, were observed under a light microscope and a scanning electron microscope. Descriptions of grain sculpture are illustrated only SEM micrographs. The studied pollen grains were medium-sized or small, spherical or prolate spheroidal. Their sculpture was always verrucate with granulation. In the studied taxa, internal apertures had the form of pores. Their number ranged from (4)5-9(14). The pores were scattered on the surface of pollen grains. Identification features of individual taxa include: presence or absence of an annulus around each pore, annulus structure, ornamentation of the pollen grain and operculum, type of aperture membrane, number of internal pores, and pore diameter. We suggest that two new pollen grain types, characteristic of <em>P. intermedia</em> and <em>P. arenaria</em>, should be distinguished, and that <em>P. alpina</em> should be assigned to the <em>P. coronopus</em> type.

scholarly journals Confusing a Pollen Grain with a Parasite Egg: an Appraisal of “Paleoparasitological Evidence of Pinworm (Enterobius Vermicularis) Infection in a Female Adolescent Residing in Ancient Tehran”

2019 ◽  
Vol 57 (6) ◽  
pp. 621-625 ◽  
Author(s):  
Morgana Camacho ◽  
Karl J. Reinhard
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Female Adolescent ◽  
Archaeological Sites ◽  
Enterobius Vermicularis ◽  
Helminth Eggs

There is often the risk of confusing pollen grains with helminth eggs from archaeological sites. Thousands to millions of pollen grains can be recovered from archaeological burial sediments that represent past ritual, medication and environment. Some pollen grain types can be similar to parasite eggs. Such a confusion is represented by the diagnosis of enterobiasis in ancient Iran. The authors of this study confused a joint-pine (<i>Ephedra</i> spp.) pollen grain with a pinworm egg. This paper describes the specific <i>Ephedra</i> pollen morphology that can be confused with pinworm eggs.

scholarly journals Pollen morphology of Amygdalus L. (Rosaceae) in Iran

2011 ◽  
Vol 79 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Mahnaz Vafadar ◽  
Farideh Attar ◽  
Hosein Maroofi ◽  
Mansur Mirtadzadini
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Pollen Grain ◽  
Type I ◽  
Type Ii ◽  
Type V ◽  
Scanning Electron ◽  
Section Level

Pollen grain of 16 species and three hybrids of the genus <em>Amygdalus</em> L., representing two subgenera and two sections distributed in Iran were examined by light and scanning electron microscopy. All pollen grains are tricolporate. The shape of grains varies from subprolate through prolate. Regarding outline, in polar view, pollen grains are triangular-circular and in equatorial view, elliptic. Regarding sculpturing of exine, the frequent type like many members of family <em>Rosaceae</em> is striate with or without perforations that can be subdivided into three subtypes: type I (A-B), type II (A-B) and type V. In three species, <em>A. trichamygdalus</em>, <em>A. spinosissima</em> and <em>A. orientalis</em>, exine sculpture type is completely different. In the first species is rugulate, in the second species is reticulate and in <em>A. orientalis</em>, it is gemmate-perforate. Ornamentation of ridges (muri) in the striate sculpture is parallel to ectocolpus. Number of perforations in tectum, diameter of them, striae intervals and the thickness of ridges varies among studied taxa. The striae have different depth and slope. Results showed that among pollen grain characters, shape is useful character solely for separating of taxonomic ranks in Iranian Amygdalus specially in subgeneric or section level.

Sign in / Sign up

Export Citation Format

Share Document