scholarly journals Genetic Differentiation Among Goats Using Randomly Amplified Polymorphic DNA (RAPD) Markers

2001 ◽  
Vol 20 (1) ◽  
pp. 83-88
Author(s):  
K. Anbarasan ◽  
A. K. Sharma ◽  
R. K. Singh ◽  
S. M. Deb ◽  
D. Sharma
Keyword(s):  
Genetic Differentiation ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna

scholarly journals Population structuring of the endemic black-cheeked gnateater, Conopophaga melanops melanops (Vieillot, 1818) (Aves, Conopophagidae), in the Brazilian Atlantic Forest

2007 ◽  
Vol 67 (4 suppl) ◽  
pp. 867-872 ◽  
Author(s):  
VO. Lunardi ◽  
MR. Francisco ◽  
PM. Galetti Jr.
Keyword(s):  
Genetic Differentiation ◽  
Atlantic Forest ◽  
Rapd Markers ◽  
Nonparametric Test ◽  
Brazilian Atlantic Forest ◽  
Randomly Amplified Polymorphic Dna ◽  
Molecular Variance ◽  
Local Populations ◽  
Population Structuring ◽  
Significant Genetic Divergence

Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic differentiation among three populations of the endemic Black-cheeked Gnateater (Conopophaga melanops melanops) within a larger pristine reminiscent of the Brazilian Atlantic Forest. Analyses of molecular variance (AMOVA) (phiST = 0.13149, P < 0.0001) and the nonparametric test for homogeneity of the molecular variance (HOMOVA) (B = 0.32337; P = 0.0019) showed a statistically significant genetic divergence among the three Black-cheeked Gnateater populations in a continuous transect of 250 km. Some hypothetic explanations for these results are the sedentary nature of the species and the historical isolation of the populations in refuges during the Pleistocene. The present results suggest that the local populations were naturally differentiated along the entire original range before the recent process of massive deforestation.

scholarly journals Genetic diversity and population structure of ginseng in China based on RAPD analysis

2016 ◽  
Vol 11 (1) ◽  
pp. 387-390
Author(s):  
Shi-jie Wang ◽  
Xiao-lin Chen ◽  
Feng-bo Han ◽  
Ru-sheng Li ◽  
Gang Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Differentiation ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Population Level ◽  
Randomly Amplified Polymorphic Dna ◽  
Significant Difference ◽  
Geographical Populations ◽  
Wild Ginseng

AbstractPopulation genetic diversity was estimated from forty-four individual ginseng (Panax ginsengC.A. Meyer) plants collected from seven geographical populations located in Heilongjiang, Liaoning, and Jilin Provinces of China as well as the People’s Republic of Korea by using randomly amplified polymorphic DNA (RAPD) markers. Overall, 41 polymorphic loci were amplified using ten primer pairs. The polymorphism percentage ranged from 50% to 100% among seven local populations of ginseng, indicating that there is plentiful genetic diversity in wild ginseng populations. The genetic diversity at the species level was higher than that at the population level. Variance analysis showed that there was a significant difference among populations in genetic diversity. The genetic differentiation coefficient (i.e., FST) indicates that 43% of the variation occurred among populations, which indicates that substantial genetic differentiation occurred among populations. At the same time, the measured value of gene flow (Nm) was 0.66 based on the observed genetic differentiation coefficient among populations, suggesting there was moderate gene flow among populations.

Randomly amplified polymorphic DNA linkage relationships in different Norway spruce populations

2001 ◽  
Vol 31 (8) ◽  
pp. 1456-1461
Author(s):  
M Troggio ◽  
T L Kubisiak ◽  
G Bucci ◽  
P Menozzi
Keyword(s):  
Rapd Markers ◽  
Rapd Marker ◽  
Population Based ◽  
Italian Population ◽  
Randomly Amplified Polymorphic Dna ◽  
Linkage Groups ◽  
Consensus Map ◽  
Norwegian Population ◽  
Marker Loci ◽  
Linkage Relationships

We tested the constancy of linkage relationships of randomly amplified polymorphic DNA (RAPD) marker loci used to construct a population-based consensus map in material from an Italian stand of Picea abies (L.) Karst. in 29 individuals from three Norwegian populations. Thirteen marker loci linked in the Italian stand did show a consistent locus ordering in the Norwegian population. The remaining 16 unlinked marker loci were spread over different linkage groups and (or) too far apart both in the population map and in this study. The limited validity of RAPD markers as genomic "hallmarks" resilient across populations is discussed. We also investigated the reliability of RAPD markers; only 58% of the RAPD markers previously used to construct the consensus map in the Italian population were repeatable in the same material. Of the repeatable ones 76.3% were amplified and found polymorphic in 29 megagametophyte sibships from three Norwegian populations.

ANALYSIS OF GENETIC DIVERSITY OF 26 CASSAVA VARIETIES (Manihot esculenta CRANTZ) WITH DIFFERENT RESPONSES TO WATERLOGGING CONDITIONS BY USING RAPD MARKERS

2021 ◽  
Vol 66 (3) ◽  
pp. 170-179
Author(s):  
Sengsoulichan Dethvongsa ◽  
Vu Nguyen Anh ◽  
Van Tran Khanh
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Flood Tolerance ◽  
Randomly Amplified Polymorphic Dna ◽  
Manihot Esculenta Crantz ◽  
Rapd Pcr ◽  
Cassava Production ◽  
Cassava Varieties

RAPD (Randomly Amplified Polymorphic DNA) is an indicator for high and stable polymorphism, widely used in the study of the diversity of cassava. In this paper, the results of using 20 polymorphic primers OPK combined with the establishment of the phylogenetic tree to analyze the genetic diversity of 26 cassava varieties with different responses to waterlogging conditions by using the RAPD-PCR technique were presented. The purpose of this experiment was to show the genetic relevance of the studied cassava varieties. The results showed that the flood tolerance of cassava was not related to the polymorphism and branching characteristics of the stem. This information may be use as a basis for selecting flood-tolerant cassava varieties for cassava production, as well as the basis for selecting genetically different parents for breeding.

Assessment of genetic variability among selected species of Apocynaceae

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Tariq Mahmood ◽  
Anna Iqbal ◽  
Nazia Nazar ◽  
Ishrat Naveed ◽  
Bilal Abbasi ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Catharanthus Roseus ◽  
Rapd Markers ◽  
Molecular Level ◽  
Ornamental Plants ◽  
Morphological Characters ◽  
Randomly Amplified Polymorphic Dna ◽  
Medicinal Uses ◽  
Alstonia Scholaris ◽  
Thevetia Peruviana

AbstractFamily Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus, were collected from Rawalpindi and Quaid-i-Azam University forest, Islamabad. To evaluate the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. A series of OPC RAPD primers were used; only six primers of OPC series gave amplification. Highest genetic variation at interspecific and intraspecific levels was shown by OPC 9 and the lowest polymorphism was observed in OPC 4. The data was analyzed by using software Statistica 5.5. In total 105 monomorphic and 272 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 26% were monomorphic and 68% were polymorphic. Low genetic diversification was observed both at intraspecific and interspecific level. At the molecular level Alstonia scholaris and Catharanthus roseus (subfamily Plumerioideae) appeared in a group and Thevetia peruviana (subfamily Rauvolfoideae) formed another group, confirming the classification based on morphological characters.

Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers

2006 ◽  
Vol 39 ◽  
pp. 77-88 ◽  
Author(s):  
S.C. Mehta ◽  
B.P. Mishra ◽  
M.S. Sahani
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Population Subdivision ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Dna Profile ◽  
Oligonucleotide Primers ◽  
Polymerase Chain ◽  
Polymorphic Bands

SummaryThe camel population in India is facing a severe decline which demands that immediate steps are taken to ensure its conservation. Characterisation is an integral part of the conservation program. The Polymerase Chain Reaction-Randomly Amplified Polymorphic DNA profile of unrelated camels of the Bikaneri (29), Jaisalmeri (30) and Kachchhi (18) breeds were analyzed. Reproducible polymorphic bands with varying frequencies among the three breeds of camel were obtained with five oligonucleotide primers. A total of 75 bands were amplified, of which 27 (36%) were polymorphic. The probability of obtaining identical fingerprints was observed to be the lowest in primer GC-10 (5.7%) followed by OP-08 (8.7%), GT-10 (11.3%), G-2 (15.5%) and G-1 (80%). Breed informative bands were amplified. The maximum genetic variability was observed in the Bikaneri (0.80±0.05) followed by the Kachchhi (0.84±0.06) and the Jaisalmeri (0.87±0.05) breeds. The inter-breed genetic distance estimates indicated a closer relationship in the Bikaneri-Kachchhi camels, (0.075), followed by the Jaisalmeri-Kachchhi (0.106) and Bikaneri-Jaisalmeri (0.132) breeds. A similar genetic relationship was observed when the degree of population subdivision was measured between the Bikaneri-Kachchhi (0.529), Jaisalmeri-Kachchhi (0.558) and Bikaneri-Jaisalmeri (0.566) breeds.

Genetic Variation Analysis of Korean Lotus ( Nelumbo nucifera ) using Randomly Amplified Polymorphic DNA ( RAPD ) Markers

1998 ◽  
Vol 28 (4) ◽  
pp. 343-355 ◽  
Author(s):  
Hyun Kim ◽  
Mi Jang Song ◽  
Ki Joomg Kim ◽  
Chong Woo Lee ◽  
Won Gil Chang ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Nelumbo Nucifera ◽  
Variation Analysis ◽  
Randomly Amplified Polymorphic Dna

Genetic diversity and relationships among Egyptian Galium (Rubiaceae) and related species using ISSR and RAPD markers

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Kadry Abdel Khalik ◽  
Magdy Abd El-Twab ◽  
Rasha Galal
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
Randomly Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeats ◽  
Close Relationship ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

AbstractGenetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.

Sign in / Sign up

Export Citation Format

Share Document