Degradation of selected pharmaceuticals detected in wastewater systems using an enzyme-mediator system and identification of resulting transformation products

Author(s):  
Ionut Daniel Caraene ◽  
Yolanta Gruchlik ◽  
Francesco Busetti ◽  
Kathryn L. Linge ◽  
Cynthia A. Joll
Author(s):  
R. Varughese ◽  
S. W. Thompson ◽  
P. R. Howell

Ever since Habraken and Economopoulos first employed the term granular bainite to classify certain unconventional transformation products in continuously cooled steels, the term has been widely accepted and used, despite the lack of a clear consensus as to the detailed nature of the transformation products which constitute granular bainite. This paper presents the preliminary results of a TEM investigation of an 0.04 wt% C, copper-containing steel (designated HSLA-100). It is suggested that the term granular ferrite rather than granular bainite is a more accurate description of this multiphase reaction product.Figure 1 is a light micrograph of a sample which had been air-cooled from 900°C to room temperature. The microstructure is typical of that which has been termed granular bainite in the past and appears to consist of equiaxed ferritic grains together with other minor transformation products. In order to examine these structures in more detail, both continuously cooled and isothermally transformed and quenched materials have been examined with TEM. Granular bainite has been found in virtually all samples.


2019 ◽  
Vol 26 (31) ◽  
pp. 31873-31886 ◽  
Author(s):  
Paula Guerra ◽  
Steven Teslic ◽  
Ariba Shah ◽  
Amber Albert ◽  
Sarah B. Gewurtz ◽  
...  
Keyword(s):  

Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 758 ◽  
Author(s):  
Jia ◽  
Sitzenfrei ◽  
Rauch ◽  
Liang ◽  
Liu

The development of urban drainage systems is challenged by rapid urbanization; however, little attention is paid to the urban form and its effects on these systems. This study develops an integrated city-drainage model that configures typical urban forms and their associated drainage infrastructures, specifically domestic wastewater and rainwater systems, to analyze the relationship between them. Three typical types of urban forms were investigated: the square, the star, and the strip. Virtual cities were designed first, with the corresponding drainage systems generated automatically and then linked to a model herein called the Storm Water Management Model (SWMM). Evaluation was based on 200 random configurations of wastewater/rainwater systems with different structures or attributes. The results show that urban forms play more important roles on three dimensions of performance, namely economic efficiency, effectiveness, and adaptability, of the rainwater systems than of the wastewater systems. Cost is positively correlated to the effectiveness of rainwater systems among the different urban forms, while adaptability is negatively correlated to the other two performance dimensions. Regardless of the form, it is difficult for a city to make its drainage systems simultaneously cost-effective, efficient, and adaptable based on the virtual cities we investigated. This study could inspire the urban planning of both built-up and to-be-built areas to become more sustainable with their drainage infrastructure by recognizing the pros and cons of different macroscale urban forms.


Toxics ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 81
Author(s):  
Kamila Šrédlová ◽  
Kateřina Šírová ◽  
Tatiana Stella ◽  
Tomáš Cajthaml

Metabolites of polychlorinated biphenyls (PCBs)—hydroxylated PCBs (OH‑PCBs), chlorobenzyl alcohols (CB‑OHs), and chlorobenzaldehydes (CB‑CHOs)—were incubated in vitro with the extracellular liquid of Pleurotus ostreatus, which contains mainly laccase and low manganese-dependent peroxidase (MnP) activity. The enzymes were able to decrease the amount of most of the tested OH‑PCBs by > 80% within 1 h; the removal of more recalcitrant OH‑PCBs was greatly enhanced by the addition of the laccase mediator syringaldehyde. Conversely, glutathione substantially hindered the reaction, suggesting that it acted as a laccase inhibitor. Hydroxylated dibenzofuran and chlorobenzoic acid were identified as transformation products of OH‑PCBs. The extracellular enzymes also oxidized the CB‑OHs to the corresponding CB‑CHOs on the order of hours to days; however, the mediated and nonmediated setups exhibited only slight differences, and the participating enzymes could not be determined. When CB‑CHOs were used as the substrates, only partial transformation was observed. In an additional experiment, the extracellular liquid of Irpex lacteus, which contains predominantly MnP, was able to efficiently transform CB‑CHOs with the aid of glutathione; mono‑ and di-chloroacetophenones were detected as transformation products. These results demonstrate that extracellular enzymes of ligninolytic fungi can act on a wide range of PCB metabolites, emphasizing their potential for bioremediation.


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