Chemical constituents, anticancer, antimicrobial and antioxidant activities of essential oil fromAnaphalis lactealgrown in Qinghai-Tibet Plateau

The antimelanogenic and antioxidant activities of the essential oil extracted from the leaves ofAcorus macrospadiceus(Yamamoto) F. N. Wei et Y. K. Li have never been explored. The essential oil effectively inhibited mushroom tyrosinase activity (EC50= 1.57 mg/mL) and B16F10 tyrosinase activity (IC50= 1.01 mg/mL), decreased the melanin content (EC50= 1.04 mg/mL), and depleted the cellular level of the reactive oxygen species (ROS) (EC50= 1.87 mg/mL). The essential oil effectively scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) (EC50= 0.121 mg/mL) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS+radicals (EC50= 0.122 mg/mL). It also exhibited an apparent reducing power (EC50= 0.021 mg/mL) and metal-ion chelating activity (EC50= 0.029 mg/mL). The chemical constituents of the essential oil are ethers (55.73%), ketones (19.57%), monoterpenes (7.82%), alcohols (3.85%), esters (3.77%), sesquiterpenes (3.72%), and aromatic compounds (2.85%). The results confirm thatA. macrospadiceusessential oil is a natural antioxidant and inhibitor of melanogenesis.

Download Full-text

Extraction, Radical Scavenging and Antimicrobial Activity of Essential Oils of Root of Dryopteris marginalis

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.22109 ◽

2019 ◽

Vol 31 (11) ◽

pp. 2512-2516 ◽

Cited By ~ 1

Author(s):

Md. Zahidul Islam ◽

Jannat Al Foisal ◽

Mahbubur Rahman ◽

Mst. Afsana Mimi ◽

Faridul Islam ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Chemical Constituents ◽

Total Concentration ◽

Petroleum Ether Extract ◽

Radical Scavenging ◽

Organic Extracts ◽

Zones Of Inhibition ◽

Essential Oil Yield

The aim of the study was to characterize the chemical constituents and determine antibacterial and antioxidant activities of essential oils and three different extracts of Dryopteris marginalis. The root had essential oil yield of 0.36 % (w/w) in which 12 organic compounds representing 97.22% of the root oils were identified. Tyranton was found as the most abundant component with 77.571 % of the total concentration in the essential oil. The zones of inhibition of different organic extracts against the tested bacteria were found in the range of 6.5-15 mm. Pseudomonas was the most vulnerable with MICs of 15.62 μg/mL by both ethyl acetate and petroleum ether extract producing 3.8 mm zone of inhibition. The essential oils extracted from roots of Dryopteris marginalis showed maximum 85.29 % inhibition of radical scavenging at 2 mg/mL concentration. Among all root extracts, methanol extract exhibited 41.11 % inhibition at 2 mg/mL concentration.

Download Full-text

Chemical Composition of Milk and Rumen Microbiome Diversity of Yak, Impacting by Herbage Grown at Different Phenological Periods on the Qinghai-Tibet Plateau

Animals ◽

10.3390/ani10061030 ◽

2020 ◽

Vol 10 (6) ◽

pp. 1030 ◽

Cited By ~ 1

Author(s):

Qingshan Fan ◽

Metha Wanapat ◽

Fujiang Hou

Keyword(s):

Chemical Composition ◽

Milk Protein ◽

Chemical Constituents ◽

Milk Composition ◽

Tibet Plateau ◽

Ruminal Fermentation ◽

Yak Milk ◽

Milk Protein Content ◽

Fermentation Parameters ◽

Qinghai Tibet Plateau

To estimate how native herbage of three different phenological periods modify rumen performance and milk quality of yak grazing alpine meadow. In this study, milk composition and the diversity of the rumen microbial community were measured in 12 full-grazing female yaks on the Qinghai-Tibet Plateau (QTP). The nutrient composition of three phenological periods was determined: Vegetative stage (VS), bloom stage (BS), and senescent stage (SS). High-throughput sequencing of the bacterial 16S rRNA gene was used. The results showed that crude protein (CP) content of herbage in BS was higher than that in vs. and SS (p < 0.05), and neutral detergent fiber (NDF) content of herbage in SS was higher than that in vs. and BS (p < 0.05). Milk solids and fat contents were higher in the vs. and SS than in BS (p < 0.05). However, milk protein content was higher for the vs. and BS than those for SS (p < 0.05). The total volatile fatty acid (VFA), acetate, and propionate concentrations were higher in vs. and BS than in SS (p < 0.05). The community richness estimates (Chao1 estimator) of vs. were higher than that in BS and the SS (p < 0.05). The diversity indices (Shannon index) of the BS were higher than that vs. and the SS (p < 0.05). Spearman correlation analysis between the milk composition, ruminal fermentation parameters, and the relative abundances of the rumen bacteria showed that milk protein content, total VFA, acetate, and propionate concentrations were positively correlated with the relative abundances of the genera Desulfovibrio, Prevotella_1, and Butyrivibrio_2 and was negatively correlated with Olsenella, Ruminococcaceae_UCG.010, and Rikenellaceae_RC9_gut_group abundances. Collectively, the results revealed that there were significant differences in nutrient composition of herbage, chemical composition of yak milk, and microbial diversity in rumen at different phenological stages. The correlations between ruminal fermentation parameters, chemical constituents of yak milk, and some genera of ruminal bacteria might be indicative that the ruminal fermentation parameters and chemical constituents of yak milk are strongly influenced by the rumen bacterial community composition.

Download Full-text

Essential Oil Composition and Bioactivities of Waldheimia glabra (Asteraceae) from Qinghai-Tibet Plateau

Molecules ◽

10.3390/molecules22030460 ◽

2017 ◽

Vol 22 (3) ◽

pp. 460 ◽

Cited By ~ 8

Author(s):

Ji De ◽

Yan Lu ◽

Lijun Ling ◽

Nan Peng ◽

Yang Zhong

Keyword(s):

Essential Oil ◽

Essential Oil Composition ◽

Tibet Plateau ◽

Oil Composition ◽

Qinghai Tibet Plateau

Download Full-text

Enzyme-assisted microwave hydro-distillation essential oil from Fructus forsythia, chemical constituents, and its antimicrobial and antioxidant activities

Food Chemistry ◽

10.1016/j.foodchem.2012.02.114 ◽

2012 ◽

Vol 134 (1) ◽

pp. 235-243 ◽

Cited By ~ 49

Author(s):

Jiao Jiao ◽

Yu-Jie Fu ◽

Yuan-Gang Zu ◽

Meng Luo ◽

Wei Wang ◽

...

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Chemical Constituents

Download Full-text

Antioxidant Activities of a New Chemotype of Piper cubeba L. Fruit Essential Oil (Methyleugenol/Eugenol): In Silico Molecular Docking and ADMET Studies

Plants ◽

10.3390/plants9111534 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1534

Author(s):

Fahad Alminderej ◽

Sana Bakari ◽

Tariq I. Almundarij ◽

Mejdi Snoussi ◽

Kaïss Aouadi ◽

...

Keyword(s):

Antioxidant Activity ◽

Molecular Docking ◽

Essential Oil ◽

Antioxidant Activities ◽

Chemical Constituents ◽

Radical Scavenging ◽

Butylated Hydroxytoluene ◽

Scavenging Activity ◽

Important Species ◽

Antioxidant Power

Piper cubeba L. fruit is an important species used in folk medicine for different types of pains such as rheumatism, chills, flu, colds, muscular aches, and fever. This study examines the chemical constituents, antioxidant activity, and potential inhibitory effect against human peroxiredoxin 5, a key enzyme of P. cubeba essential oil from fruits. Using gas chromatography coupled with mass spectrometry (GC–MS), the principal components were methyleugenol (41.31%) and eugenol (33.95%), followed by (E)-caryophyllene (5.65%), p-cymene-8-ol (3.50%), 1,8-cineole (2.94%), and α-terpinolene (1.41%). Results showed similar scavenging activity via 2,2-diphenyl-1-picrylhydrazyl DPPH radical scavenging activity (IC50 = 110.00 ± 0.08 μg/mL), as well as very potent antioxidant activity against both ferric reducing/antioxidant power (FRAP) (106.00 ± 0.11 μg/mL) and β-carotene bleaching (IC50 = 315.00 ± 2.08 μg/mL) assays when compared to positive butylated hydroxytoluene and ascorbic acid. The molecular docking approach has also been performed to screen the antioxidant activities of the major and potent compounds against human protein target peroxiredoxin 5. Results showed good binding profiles and attributed the strongest inhibitory activity to β-caryophyllene oxide (–5.8 kcal/mol), followed respectively by isocembrol and α-selinene (–5.4 kcal/mol), and viridiflorol (–5.1 kcal/mol). Furthermore, ADME (absorption, distribution, metabolism and excretion)-related physicochemical and pharmacokinetic properties have been assessed and support our in vitro findings. This work demonstrates the powerful antioxidant potency of cubeba pepper and paves the way for the discovery and development of antioxidant agent with high potency.

Download Full-text

Chemical Composition, Antioxidant and Inhibitory Effect of Cupressus sempervirens Essential Oils and Methanolic Extract on Beta-lactamase producing Isolates

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00812 ◽

2021 ◽

pp. 4673-4679

Author(s):

Haitham Qaralleh ◽

Khaled M Khleifat ◽

Ali M Khlaifat ◽

Muhamad Al-limoun ◽

Nafe M Al-Tawarah ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Methanol Extract ◽

Antioxidant Activities ◽

Micrococcus Luteus ◽

Chemical Constituents ◽

Major Constituent ◽

Klebsiella Pneumonia ◽

Cupressus Sempervirens ◽

Antioxidant Efficiency

The Jordanian endemic medicinal plant, Cupressus sempervirens was obtained from Dhana Natural Reserve, Al-Tafilah, Jordan to investigate its antibacterial and antioxidant activities. The procuring of essential oil was made by processing of dry leaves of C. sempervirens using steam-distillation method giving 0.26% (w/w) yield. The analysis of obtained EO for its chemical constituents, was achieved by GC-MS. The equivalent of 94.02% of the entire EO has been extracted and consists of twenty-two compounds. The characterization of EO was made by their presence of three groups of chemical compounds namely Sesquiterpene hydrocarbons (71.0%), Oxygenated sesquiterpenes (11.5%) and Monoterpenes hydrocarbons (10.6%). The major constituent was germacrene-D (14.2%) along with the d-cadinene (11.0%), ß-pinene (10.0%) and isocedrol (9.8%). This conferring a chemotaxonomic value as well as a higher degree of polymorphism in the occurrence of these compounds in C. sempervirens as compared with the same species in different location worldwide. The efficiency of methanol extract and essential oil as antibacterial was evaluated against nine bacteria, using disc diffusion and MIC methods. Results showed that the methanol extract at 2000µg/disc of C. sempervirens caused the growth inhibition of Bacillus subtilis, Micrococcus luteus, Staphylococcus epidermidis and Staphylococcus aureus and producing inhibition zone ranges between 12-15mm. The MIC values recorded by essential oils of C. sempervirens were as follow: S. epidermidis and S. aureus (370µg/mL), Escherichia coli and Enterobacter aerogenes (1000µg/mL), Pseudomonas aeruginosa (2000µg/mL) and M. luteus, Klebsiella pneumonia and Salmonella typhi (3000µg/mL). In the present study, C. sempervirens extract exerted antioxidant efficiency with an IC50 value of 27.31µg/mL.

Download Full-text