Phytochemical investigation and anticholinesterase activity of ethyl acetate fraction of Myristica fragrans Houtt. seeds

2020 ◽  
pp. 1-7 ◽  
Author(s):  
Sahar Omidpanah ◽  
Yasaman Vahedi-Mazdabadi ◽  
Azadeh Manayi ◽  
Arezoo Rastegari ◽  
Roshanak Hariri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Anticholinesterase Activity ◽  
Myristica Fragrans ◽  
Phytochemical Investigation ◽  
Myristica Fragrans Houtt

scholarly journals Non-alkaloid Constituents from Mahonia bealei

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Bui Van Thanh ◽  
Nguyen Thi Van Anh ◽  
Do Hoang Giang ◽  
Nguyen Hai Dang ◽  
Luu Dam Ngoc Anh ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Spectroscopic Methods ◽  
Phytochemical Investigation ◽  
Mahonia Bealei ◽  
New Compounds

A phytochemical investigation of an ethyl acetate fraction of Mahonia bealei trunks led to the isolation of three new and four known compounds. By means of NMR and MS spectroscopic methods, the structure of the new compounds was elucidated as bishexadecyl epiphyllate (1), 3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxy-2-sulfopropane-1-ol (2), and 6α-acetoxystigmastan-3β-ol (3).

scholarly journals Antimycobacterial and Nitric Oxide Production Inhibitory Activities ofOcotea notatafrom Brazilian Restinga

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Isabela Francisca Borges Costa ◽  
Sanderson Dias Calixto ◽  
Marlon Heggdorne de Araujo ◽  
Tatiana Ungaretti Paleo Konno ◽  
Luzineide Wanderley Tinoco ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
National Park ◽  
Antimycobacterial Activity ◽  
Ethyl Acetate Fraction ◽  
No Production ◽  
Nitric Oxide Production ◽  
Phytochemical Investigation ◽  
Inhibitory Activities ◽  
Cox 2 ◽  
Cox 1

The genusOcotea(Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus asO. puberulaandO. quixoshave been described in the literature, showing antibacterial activity. AndOcotea macrophyllashowed anti-inflammatory activity with inhibition of COX-1, COX-2, and LOX-5. The purpose of this study was the phytochemical investigation of the plant speciesOcotea notatafrom Restinga Jurubatiba National Park, Macaé, RJ, Brazil, and the search for antimycobacterial fractions and compounds. The crude extract was evaluated for antimycobacterial activity and presented95.75±2.53% of growth inhibition at 100 µg/mL. Then, it was subjected to a liquid-liquid partition and subsequently was chemically investigated by HPLC, revealing the major presence of flavonoids. In this process the partition fractions hexane, ethyl acetate, and butanol are shown to be promising in the antimycobacterial assay. In addition, ethyl acetate fraction was chromatographed and afforded two flavonoids identified by MS and NMR as afzelin and isoquercitrin. The isolated flavonoids afzelin and isoquercitrin were evaluated for their antimycobacterial activity and for their ability to inhibit NO production by macrophages stimulated by LPS; both flavonoids isoquercitrin (Acet22) and afzelin (Acet32) were able to inhibit the production of NO by macrophages. The calculated IC50of Acet22 and Acet32 was 1.03 and 0.85 µg/mL, respectively.

scholarly journals Potent Acetylcholinesterase Inhibitory Compounds from Myristica fragrans

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
To Dao Cuong ◽  
Tran Manh Hung ◽  
Hyoung Yun Han ◽  
Hang Sik Roh ◽  
Ji-Hyeon Seok ◽  
...  
Keyword(s):  
Cholinesterase Activity ◽  
Ethyl Acetate Fraction ◽  
Chemical Structures ◽  
Spectroscopic Analyses ◽  
Nmr Data ◽  
Myristica Fragrans ◽  
Inhibitory Compounds ◽  
Effective Activity ◽  
Myristica Fragrans Houtt ◽  
Anticholinesterase Properties

The anti-cholinesterase activity was evaluated of the ethyl acetate fraction of the methanol extract of Myristica fragrans Houtt (Myristicaceae) seeds and of compounds isolated from it by various chromatographic techniques. The chemical structures of the compounds were determined from spectroscopic analyses (NMR data). Thirteen compounds (1—13) were isolated and identified. Compound 8 {[(7S)-8′-(4′-hydroxy-3′-methoxyphenyl)-7-hydroxypropyl]benzene-2,4-diol} showed the most effective activity with an IC50 value of 35.1 μM, followed by compounds 2 [(8R,8′S)-7′-(3′,4′-methylenedioxyphenyl)-8,8′-dimethyl-7-(3,4-dihydroxyphenyl)-butane] and 11 (malabaricone C) with IC50 values of 42.1 and 44.0 μM, respectively. This is the first report of significant anticholinesterase properties of M. fragrans seeds. The findings demonstrate that M. fragrans could be used beneficially in the treatment of Alzheimer's disease.

scholarly journals ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

2019 ◽  
Vol 19 (3) ◽  
pp. 58-63
Author(s):  
Ilham Maulana ◽  
Binawati Ginting ◽  
Nurdin Nurdin ◽  
Saiful Fakri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Myristica Fragrans ◽  
Ic50 Value ◽  
Stem Bark Extract ◽  
Myristica Fragrans Houtt

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared  to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

scholarly journals CNS Activity of Myristica fragrans Houtt. - An Experimental Study

2018 ◽  
Vol 17 (1) ◽  
pp. 98-106
Author(s):  
Ankita Mishra ◽  
Syed Ziaur Rahman ◽  
Rahat Ali Khan
Keyword(s):  
Ethyl Acetate ◽  
Open Field ◽  
Medical Science ◽  
Ethanolic Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Tail Flick Test ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Objective: To evaluatethe role of Myristica fragrans Houtt. for its analgesic, sedative and anxiogenic activity.Method: Charles Foster rats were administered orally the ethanolic extract (EEMF) and fractions of extract in ethyl acetate (EAMF), chloroform (CMF), and n-hexane (HMF). They were screened for sedative activity using Pentobarbitone sleep potentiation test, analgesic activity using Eddy’s hot plate and Tail flick test; and anxiogenic or anxiolytic activity using Open field test.Result: In pentobarbitone induced sleep potentiation test, the ethanolic extract (EEMF) and ethyl acetate fraction (EAMF) showed significant decrease in latency of sleep and highly significant increase in duration of sleep. In Open field test, EEMF, EAMF and HMF showed highly significant decrease in locomotion parameters as shown by decrease in rearing, preening and ambulation. In Eddy’s hot plate test and Tail flick test, highly significant increase in reaction time was seen in EEMF, EAMF, HMF groups.Conclusion: The observed results suggest that Myristica fragrans has analgesic, anxiogenic and sedative activity.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.98-106

scholarly journals Sterculia diversifolia bears anti-cancer and immunomodulatory activities

2017 ◽  
Vol 12 (1) ◽  
pp. 51 ◽  
Author(s):  
Fazle Rabbi ◽  
Amir Zada ◽  
Achyut Adhikari ◽  
Almas Jabeen ◽  
Amna Nisar ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Significant Activity ◽  
Immunomodulatory Agents ◽  
Phytochemical Investigation ◽  
Anti Cancer ◽  
Cancer Activity ◽  
Soluble Fractions

<p class="Abstract">The present study was aimed to evaluate the methanolic extract and subsequent solvents soluble fractions of <em>Sterculia </em>diversifolia bark for cytotoxic, anti-cancer and immunomodulatory activities. Phytochemical investigation confirmed the presence of alkaloids, flavonoids, saponins, glycosides, etc. In the cytotoxic activity, n-hexane showed potent activity (LD<sub>50</sub>: 7.0 µg/mL) followed by dichloromethane fraction (LD<sub>50</sub>: 16.2 µg/mL). In the anti-cancer activity, dichloromethane fraction showed potent activity (IC<sub>50</sub>: 5.9 µg/mL) followed by ethyl acetate fraction (IC<sub>50</sub>: 9.5 µg/mL). While in the immunomodulatory assay, ethyl acetate fraction showed a very significant activity (IC<sub>50</sub>: 21.0 µg/mL) followed by dicloromethane and n-butanol fractions (IC<sub>50</sub>: 25.0 and 25.3 µg/mL respectively). Hence, it is clear that <em>S. </em>diversifolia has anti-cancer and immunomodulatory agents.</p>

scholarly journals PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID

2017 ◽  
Vol 10 (11) ◽  
pp. 70
Author(s):  
Omer H Ahmed ◽  
Maha N Hamad ◽  
Noor S Jaafar
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Absolute Methanol ◽  
Isolation And Identification ◽  
Chenopodium Murale ◽  
Phytochemical Investigation

  Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.

scholarly journals PROTECTING OUTCOME OF ETHYL ACETATE FRACTION OF AQUEOUS-METHANOL: 40:60 EXTRACT OF SEED OF MYRISTICA FRAGRANS HOUTT. IN STREPTOZOTOCIN-INDUCED DIABETIC RAT: A DOSE DEPENDENT STUDY

2018 ◽  
Vol 10 (9) ◽  
pp. 54
Author(s):  
Ankita Dey ◽  
Adrija Tripathy ◽  
Debidas Ghosh
Keyword(s):  
Serum Insulin ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Aqueous Methanol ◽  
Myristica Fragrans ◽  
End Products ◽  
Myristica Fragrans Houtt ◽  
Dose Dependent ◽  
Different Doses

Objective: The investigation has been conducted to find out the threshold dose of ethyl acetate fraction prepared from aqueous-methanol (40:60) extract of seed of Myristica fragrans (Houtt.) from the dose dependent experiment by noting the remedial effects of the said fraction on different complications developed in streptozotocin induced diabetic rat.Methods: Diabetic condition was made by single intramascular injection of streptozotocin at a dose of 4 mg/0.1 ml citrate buffer/100 g body weight. Treatment was conducted at different doses for 28 d on diabetic rat. Efficacy of the fraction on fasting blood glucose (FBG) and serum insulin levels, activities of key carbohydrate metabolic enzymes such as hexokinase, glucose-6-phosphate dehydogenase, glucose-6-phosphatase of hepatic and skeletomuscular tissue as well as antioxidative enzymes like catalase, superoxide dismutase (SOD) in connection with the levels of oxidative stress end products such as thiobarbituric acid reactive substances (TBARS), conjugated diene (CD) in hepatic and renal tissue and gene expression of hexokinase-1, SOD, Bax, Bcl-2 in hepatic tissue were assessed.Results: After different doses of fraction treatment to the diabetic animals; FBG, serum insulin levels and activities of carbohydrate metabolic enzymes i.e. hexokinase, glucose-6-phosphate dehydrogenase and glucose-6-phosphatase were rectified towards the control at the level of p<0.05. There is a significant (p<0.05) recovery in the activities of antioxidant enzymes such as catalase and SOD in respect to the vehicle treated diabetic group. Oxidative stress end products such as CD and TBARS levels were increased significantly (p<0.05) in vehicle treated diabetic rats and was rectified significantly (p<0.05) after fraction treatment at the dose of 10 mg or 20 mg or 40 mg fraction treated group. Gene expression of hexokinase-1, superoxide dismutase, bax and bcl-2 was rectified towards vehicle treated control significantly at the level of p<0.05 after treatment of different doses of fractions to the diabetic animals.Conclusion: The study indicates that the seeds of Myristica fragrans at the dose of 20 mg/100 g body weight possess most effective outcome to manage diabetic disorders in streptozotocin induced diabetic rats.

Bioactivities of Methanol and Ethyl Acetate Mace Extracts of Myristica fragrans Houtt

2018 ◽  
Vol 8 (3) ◽  
pp. 103-107 ◽  
Author(s):  
Adelina Simamora ◽  
Adit Widodo Santoso ◽  
Kris Herawan Timotius
Keyword(s):  
Ethyl Acetate ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Chemical Constituents and Biological Activities of Galinsoga parvifl ora Cav. (Asteraceae) from Egypt

2013 ◽  
Vol 68 (7-8) ◽  
pp. 285-292 ◽  
Author(s):  
Islam Mostafa ◽  
Ehsan Abd El-Aziz ◽  
Samia Hafez ◽  
Assem El-Shazly
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Enzyme Level ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Phytochemical Investigation

The phytochemical investigation of an aqueous ethanolic extract of Galinsoga parviflora Cav. (Asteraceae) resulted in the isolation and identification of eleven compounds namely: triacontanol, phytol, β-sitosterol, stigmasterol, 7-hydroxy-β-sitosterol, 7-hydroxystigmasterol, β-sitosterol-3-O-β-D-glucoside, 3,4-dimethoxycinnamic acid, protocatechuic acid, fumaric acid, and uracil. Furthermore, 48 volatile constituents were identified in the hydrodistilled oil of the aerial parts. The ethanolic extract at a content of 400 mg/kg body weight (BW) exerted 87% reduction in the alanine aminotransferase enzyme level in cirrhotic rats compared with the standard silymarin (150 mg/kg BW) and also exerted a reduction in the blood glucose level equivalent to that of glibenclamide (5 mg/kg BW) in diabetic rats. The ethanolic extract, light petroleum and ethyl acetate fractions exhibited substantial antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Aspergillus niger, and Candida albicans. The ethyl acetate fraction showed strong antioxidant activity at a concentration of 150 mg/mL as compared with 0.1 M ascorbic acid. The cytotoxic effect against the MCF-7 cell line was found to be weak

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