The Stability of Citrate-Capped Silver Nanoparticles in Isotonic Glucose Solution for Intravenous Injection

2013 ◽  
Vol 76 (22) ◽  
pp. 1236-1245 ◽  
Author(s):  
Kwangsik Park ◽  
Yeonjin Lee
2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Asra Parveen ◽  
Manjunath S. Yalagatti ◽  
Venkataraman Abbaraju ◽  
Raghunandan Deshpande

Antimicrobial study of biofunctionalized silver nanoparticles has been done with the emphasis on its mechanism on both gram positive and negative bacteria. The biofunctionalized silver nanoparticles are employed considering their importance in green chemistry with respect to easy synthesis, usefulness, and economic synthetic procedure involved. The stability of these nanoparticles was determined by zeta potential analyzer. The probable mechanism of antibacterial activity was performed on Proteus mirabilis by field emission scanning electron microscopy (FESEM) and energy dispersive spectroscopy (EDAX) study which does not show the presence of silver. The free radicals generated by silver nanoparticles were responsible for lethal antibacterial activity by rupturing the cell surface which causes improper nutrient and signal supply. Free radical scavenging efficacy of silver nanoparticles was confirmed by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method. AgNP enhanced the membrane leakage of reducing sugars by destroying the proteins existing on the cell wall. These nanoparticles are found to be toxic against human pathogens and are highly effective on Staphylococcus aureus. The effect of silver nanoparticles is concentration dependent and independent of the type of strains used.


NANO ◽  
2021 ◽  
pp. 2150088
Author(s):  
Kalthoum Chourabi ◽  
Lobna Elleuch ◽  
Salma Kloula ◽  
Ahmed Landoulsi ◽  
Abdelwaheb Chatti

Silver nanoparticles have attracted much interest from scientists to develop nanosilver-based disinfectant products due to their unique properties of high antimicrobial activity. This study focused on biosynthesis, characterization, antimicrobial and antibiofilm effects of silver nanoparticles against vegetative and starved Shigella strains. The silver nanoparticles were synthesized using the yeast Yarrowia lipolytica and characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial and antibiofilm activities of silver nanoparticles were tested against the growth of vegetative and starved Shigella strains. After the addition of silver nitrate solution to the supernatant of Y. lipolytica, we noticed the appearance of a brown-black coloration that suggested the formation of silver nanoparticles. The presence of silver nanoparticles was manifested by a maximum absorption in the ultraviolet–visible range, precisely at the wavelength 420[Formula: see text]nm. The crystalline nature and the stability of silver nanoparticles were confirmed, respectively, by XRD and FTIR analysis. The antibacterial activity of silver nanoparticles showed significant toxicity on Shigella strains indicating that the starved cells were more sensitive to treatment with silver nanoparticles than vegetative cells. Surprisingly, the biofilm formation had not been inhibited by silver nanoparticles for both vegetative and starved cells. In conclusion, a new class of nanosilver containing disinfectant nanoproducts will be promising for advanced environmental treatments including air disinfection, water disinfection, surface disinfection and personal hygiene that will help to prevent the further outbreak of diseases.


Author(s):  
Mohib Shah ◽  
Natasha Anwar ◽  
Samreen Saleem ◽  
Iqbal Munir ◽  
Niaz Ali Shah ◽  
...  

Background. Nanotechnology is promising field for generating new applications. A green synthesis of nanoparticles through biological methods using plant extract have a reliable and ecofriendly approach to improve our global environment. Methods. Silver nanoparticles (AgNPs) were synthesized using aqueous extract of Anagalis arvensis L and silver nitrate and were physicochemically characterized. Results. The stability of AgNPs toward acidity, alkalinity, salinity and temperature showed that they remained stable at room temperature for more than two months. The SEM and TEM analysis of the AgNPs showed that they have a uniform spherical shape with an average size in the range of 40–78 nm. Further 1-Dibhenyl-2-Picrylhydrazl radical in Anagalis arvensis L.mediated AgNPs showed a maximum activity of 98% at concentration of 200μg/mL. Hydrogen peroxide scavenging assay in Anagalis arvensis L. mediated AgNPs showed a maximum activity of 85% at concentration of 200μg/mL. Reducing power of Anagalis arvensis L.Ag NPs exhibited a higher activity of 330 μg/mL at concentration of 200 μg/mL. These NPs have cytotoxic effects against brine shrimp (Artemia salina) nauplii with a value of 53% LD 178.04μg/mL. Conclusion. The AgNPs synthesized using Anagalis arvensis L. extract demonstrate a broad range of applications.


2018 ◽  
Vol 92 (4) ◽  
pp. 1393-1405 ◽  
Author(s):  
Ji Hyun Lee ◽  
Jae Hyuck Sung ◽  
Hyun Ryol Ryu ◽  
Kyung Seuk Song ◽  
Nam Woong Song ◽  
...  

2020 ◽  
Vol 21 (13) ◽  
pp. 4647 ◽  
Author(s):  
Elżbieta Pędziwiatr-Werbicka ◽  
Michał Gorzkiewicz ◽  
Katarzyna Horodecka ◽  
Viktar Abashkin ◽  
Barbara Klajnert-Maculewicz ◽  
...  

Gene therapy is a promising approach in cancer treatment; however, current methods have a number of limitations mainly due to the difficulty in delivering therapeutic nucleic acids to their sites of action. The application of non-viral carriers based on nanomaterials aims at protecting genetic material from degradation and enabling its effective intracellular transport. We proposed the use of silver nanoparticles (AgNPs) surface-modified with carbosilane dendrons as carriers of anticancer siRNA (siBcl-xl). Using gel electrophoresis, zeta potential and hydrodynamic diameter measurements, as well as transmission electron microscopy, we characterized AgNP:siRNA complexes and demonstrated the stability of nucleic acid in complexes in the presence of RNase. Hemolytic properties of free silver nanoparticles and complexes, their effect on lymphocyte proliferation and cytotoxic activity on HeLa cells were also examined. Confocal microscopy proved the effective cellular uptake of complexes, indicating the possible use of this type of silver nanoparticles as carriers of genetic material in gene therapy.


2018 ◽  
Vol 4 (12) ◽  
pp. 2146-2159 ◽  
Author(s):  
Ivona Capjak ◽  
Maja Zebić Avdičević ◽  
Maja Dutour Sikirić ◽  
Darija Domazet Jurašin ◽  
Amela Hozić ◽  
...  

pH, electrolytes and surfactants affected the stability of AgNPs in artificial test water system.


Author(s):  
Robert Lotha ◽  
Aravind Sivasubramanian ◽  
Meenakshi Sundaram Muthuraman

Objective: The present study was aimed at the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Euphorbia cyathophora leavesand testing their anticancer potential using HT-29 cell line model.Methods: Green synthesis of silver nanoparticles was obtained with the aqueous extract of E. cyathophora. The synthesized nanoparticles wereconfirmed initially by ultraviolet-visible spectroscopy. Further, scanning electron microscopy, transmission electron microscopy, and X-Ray diffractionstudies also ensured the presence of silver nanoparticles. Zeta potential studies revealed the stability of the silver nanoparticles.Results: Antioxidant and anticancer studies of the nanoparticles against HT-29 cell line exhibited remarkable results.Conclusion: This ensures that the synthesized nanoparticles play an important role in medicinal biology.


2019 ◽  
Vol 55 (61) ◽  
pp. 8935-8938 ◽  
Author(s):  
Nizar Akermi ◽  
Hela Mkaouar ◽  
Aicha Kriaa ◽  
Amin Jablaoui ◽  
Souha Soussou ◽  
...  

Eubacterium saburreum serpin from human gut microbiota inhibits the pancreatic elastase associated with pancreatitis, inhibition is strongly increased by para-sulphonato-calix[8]arene silver nanoparticles.


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