Synthesis of Silver Nanoparticles from Anagalis arvensis and Their Biomedical Applications

Background. Nanotechnology is promising field for generating new applications. A green synthesis of nanoparticles through biological methods using plant extract have a reliable and ecofriendly approach to improve our global environment. Methods. Silver nanoparticles (AgNPs) were synthesized using aqueous extract of Anagalis arvensis L and silver nitrate and were physicochemically characterized. Results. The stability of AgNPs toward acidity, alkalinity, salinity and temperature showed that they remained stable at room temperature for more than two months. The SEM and TEM analysis of the AgNPs showed that they have a uniform spherical shape with an average size in the range of 40–78 nm. Further 1-Dibhenyl-2-Picrylhydrazl radical in Anagalis arvensis L.mediated AgNPs showed a maximum activity of 98% at concentration of 200μg/mL. Hydrogen peroxide scavenging assay in Anagalis arvensis L. mediated AgNPs showed a maximum activity of 85% at concentration of 200μg/mL. Reducing power of Anagalis arvensis L.Ag NPs exhibited a higher activity of 330 μg/mL at concentration of 200 μg/mL. These NPs have cytotoxic effects against brine shrimp (Artemia salina) nauplii with a value of 53% LD 178.04μg/mL. Conclusion. The AgNPs synthesized using Anagalis arvensis L. extract demonstrate a broad range of applications.

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Green synthesis and stabilization of earthworm-like gold nanostructure and quasi-spherical shape using Caesalpinia sappan Linn. extract

Green Processing and Synthesis ◽

10.1515/gps-2017-0090 ◽

2018 ◽

Vol 7 (5) ◽

pp. 424-432 ◽

Cited By ~ 1

Author(s):

Widsanusan Chartarrayawadee ◽

Chee O. Too ◽

Sukunya Ross ◽

Gareth M. Ross ◽

Kanlaya Jumpatong ◽

...

Keyword(s):

Zeta Potential ◽

Green Synthesis ◽

Spherical Shape ◽

Colloidal Solutions ◽

Tem Analysis ◽

Caesalpinia Sappan ◽

Synthesis Of Nanoparticles ◽

Stabilizing Agents ◽

Transmission Electron ◽

The Stability

AbstractGreen synthesis of nanoparticles (NPs) has received much attention due to biocompatibility and being facile and eco-friendly.Caesalpinia sappanLinn. (CS) is a plant found in Southeast Asia and is commonly known as sappan wood. In this research, we are the first to successfully use CS extract as reducing and stabilizing agents for the green synthesis of gold NPs (AuNPs). Colloidal solutions of AuNPs after reduction show natural red-wine and dark purple color depending on the size of AuNPs, without the interference of the orange/reddish-orange color from the CS extract. The concentration of CS was investigated for the formation and stabilization of AuNPs in colloidal solution (i.e. morphology, size and zeta potential). It was found that the optimized concentration of the reducing agent for the green synthesis of AuNPs was in the range of 0.004–0.04 wt% of CS. As the concentration of CS was increased, the sizes of AuNPs decreased due to the adsorption and stabilizing effect of CS. Transmission electron microscopy (TEM) analysis showed differences in size and shape of the NPs with earthworm and quasi-spherical characteristics. The zeta potential of AuNPs synthesized in the presence of CS was approximately in the range of −10 mV to −20 mV, indicating the stability of AuNPs synthesized by CS.

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Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽

10.3390/biology10060473 ◽

2021 ◽

Vol 10 (6) ◽

pp. 473

Author(s):

Prabu Kumar Seetharaman ◽

Rajkuberan Chandrasekaran ◽

Rajiv Periakaruppan ◽

Sathishkumar Gnanasekar ◽

Sivaramakrishnan Sivaperumal ◽

...

Keyword(s):

Silver Nanoparticles ◽

Culture Filtrate ◽

Morphological Changes ◽

Xrd Analysis ◽

Penicillium Oxalicum ◽

Breast Cancer Cell Lines ◽

Face Centered Cubic ◽

Tem Analysis ◽

X Ray ◽

Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

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Síntese, caracterização e estabilidade de nanopartículas de magnetita

Latin American Journal of Development ◽

10.46814/lajdv3n4-075 ◽

2021 ◽

Vol 3 (4) ◽

pp. 2738-2749

Author(s):

Monise Cristina Ribeiro Casanova Coltro ◽

Warde Antonieta da Fonseca-Zang ◽

Joachim Werner Zang ◽

Danilo César Silva e Sousa

Keyword(s):

New Technology ◽

X Ray Diffraction ◽

Synthesis Of Nanoparticles ◽

Research Areas ◽

Magnetite Fe3o4 ◽

Mineral Substance ◽

Abundant Element ◽

The Stability ◽

Average Size

Nanopartículas de ferro são muito utilizadas em diversas áreas de pesquisa. O elemento químico ferro (Fe), sendo o quarto elemento mais abundante na crosta terrestre, e a substância mineral magnetita, com propriedade magnética, apresentam aplicações nas áreas industrial, ambiental, biomédica e de novas tecnologias. Este trabalho apresenta processo de síntese de nanopartículas partindo-se de sais precursores, bem como a caracterização dos produtos e as rotas para estabilizá-los. Os sais químicos precursores utilizados foram o cloreto férrico (FeCl3) e o sulfato ferroso (FeSO4) na proporção de 2:1, sob agitação por ultrassom e pH ácido. Para formação do precipitado de nanopartículas usou-se solução aquosa de hidróxido de sódio (NaOH) de pH 12. A difratometria de raio-X, mostra a presença de magnetita (Fe3O4) indicada pelos picos característicos de difração em graus 2Ө = 18° (largo), 31° (fino), 36° (bem definido), 43,4°, 45°, 53,6°, 57,7°, 63,3°. A microscopia eletrônica de transmissão mostra a morfologia dos produtos da síntese. Fatores que influenciam a estabilidade das partículas são agitação, o ajuste de pH, condições de secagem. O tamanho médio das nanopartículas de magnetitas é de aproximadamente 15 nm. Iron nanoparticles are widely used in several research areas. The chemical element iron (Fe), being the fourth most abundant element in the earth's crust, and the mineral substance magnetite, with magnetic properties, have applications in industrial, environmental, biomedical, and new technology areas. This work presents the process of synthesis of nanoparticles starting from precursor salts, as well as the characterization of the products and the routes to stabilize them. The precursor chemical salts were ferric chloride (FeCl3) and ferrous sulfate (FeSO4) in a 2:1 ratio, under ultrasound agitation and acidic pH. For the nanoparticles growth was applied aqueous solution of sodium hydroxide (NaOH) at pH 12. X-ray diffraction shows the presence of magnetite (Fe3O4) indicated by characteristic diffraction peaks in degrees 2Ө = 18° (wide), 31° (fine), 36° (well defined), 43.4°, 45°, 53.6°, 57.7°, 63.3°. Scanning electron microscopy shows the morphology of the synthesis products. Factors that influence the stability of the particles are agitation, the pH adjustment, and the conditions of drying. The average size of the magnetite nanoparticles is approximately 15 nm.

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Silver Nanoparticles Formation by Jatropha integerrima and LC/MS-QTOF-Based Metabolite Profiling

Nanomaterials ◽

10.3390/nano11092400 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2400

Author(s):

Afrah E. Mohammed ◽

Lamya Ahmed Al-Keridis ◽

Ishrat Rahman ◽

Modhi O. Alotaibi ◽

Rasha Saad Suliman ◽

...

Keyword(s):

Silver Nanoparticles ◽

Laser Scanning ◽

Antibacterial Effect ◽

Therapeutic Potential ◽

Laser Scanning Microscopy ◽

Ultrastructural Changes ◽

Confocal Laser ◽

Synthesis Methods ◽

Tem Analysis ◽

Average Size

The broad application of metal nanoparticles in different fields encourages scientists to find alternatives to conventional synthesis methods to reduce negative environmental impacts. Herein, we described a safe method for preparing silver nanoparticles (J-AgNPs) using Jatropha integerrima leaves extract as a reducing agent and further characterize its physiochemical and pharmacological properties to identify its therapeutic potential as a cytotoxic and antimicrobial agent. The biogenic synthesized J-AgNPs were physiochemically characterized by ultraviolet-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM), and energy-dispersive X-ray spectroscopy. HPLC-DAD, followed by LC/MS and the Fourier-transform infrared spectroscopy (FTIR), was applied to detect the biomolecules of J. integerrima involved in the fabrication of NPs. Furthermore, J-AgNPs and the ampicillin-nanocomposite conjugate were investigated for their potential antibacterial effects against four clinical isolates. Finally, cytotoxic effects were also investigated against cancer and normal cell lines, and their mechanism was assessed using TEM analysis and confocal laser scanning microscopy (LSM). Ag ions were reduced to spherical J-AgNPs, with a zeta potential of −34.7 mV as well as an average size of 91.2 and 22.8 nm as detected by DLS and TEM, respectively. HPLC GC/MC analysis identified five biomolecules, and FTIR suggested the presence of proteins besides polyphenolic molecules; together, these molecules could be responsible for the reduction and capping processes during NP formation. Additionally, J-AgNPs displayed a strong antibacterial effect, although the ampicillin conjugated form had a very weak antibacterial effect. Furthermore, the NPs caused a reduction in cell viability of all the treated cells by initiating ultrastructural changes and apoptosis, as identified by TEM and LSM analysis. Therefore, J-AgNPs can be formed using the leaf extract from the J. integerrima plant. Furthermore, J-AgNPs may serve as a candidate for further biochemical and pharmacological testing to identify its therapeutic value.

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A Novel Green Biogenic Synthesis of Silver Nanoparticles using Tabebuia rosea (Bertol.) DC Fruit Extract and Its Antioxidant and Antibacterial Potential

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.1.8 ◽

2021 ◽

Vol 14 (1) ◽

pp. 5323-5333

Author(s):

Dr. Guru Kumar Dugganaboyana ◽

Ramya Jayendra ◽

Arpitha Narayan ◽

Meghana Siddappa Konasur

Keyword(s):

Silver Nanoparticles ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Fruit Extract ◽

Synthesis Of Nanoparticles ◽

Drug Molecules ◽

Agar Disc ◽

Tabebuia Rosea ◽

Average Size ◽

Antibacterial Potential

Plant based synthesis of nanoparticles has generated worldwide interest because of cost-effectiveness, eco-friendly nature and abundance of applications. In the present investigation , antimicrobial potential of silver nanoparticles (AgNPs) of aqueous extract of Tabebuia rosea (Bertol.) DC (T. rosea) fruit extract has been investigated. Agar disc diffusion method was used for determining the antimicrobial activity of selected aqueous fruit extract AgNPs. Phytochemical analysis of aqueous fruit extract of T. rosea fruit revealed the presence of alkaloids, flavonoids, tannins, phenols, carbohydrates, glycosides, Vitamin-C, proteins and terpenoids. AgNPs synthesis using T. rosea aqueous fruit extract and characterized by UV-Visible spectroscopy showed a peak at 420 nm and average size of 82.9 nm, FT-IR analysis, dynamic light scattering, scanning electron microscope, EDX and X-ray diffraction analysis. Evaluation of antibacterial activity of green synthesized AgNPs recorded the more potent activity against selected human bacterial pathogens. The results obtained indicated that the fruit extract of T. rosea as well as AgNPs have strong and effective antibacterial potential that provide marvelous source for the development of new drug molecules of herbal origin which may be used for the welfare of humanity.

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Green Synthesis of Silver Nanoparticles and Their Bactericidal and Antimycotic Activities against Oral Microbes

Journal of Nanomaterials ◽

10.1155/2016/9204573 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Osvelia E. Rodríguez-Luis ◽

Rene Hernandez-Delgadillo ◽

Rosa Isela Sánchez-Nájera ◽

Gabriel Alejandro Martínez-Castañón ◽

Nereyda Niño-Martínez ◽

...

Keyword(s):

Silver Nanoparticles ◽

Fungal Growth ◽

Spherical Shape ◽

Xrd Analysis ◽

Glycyrrhiza Glabra ◽

X Ray Diffraction ◽

Synthesize Silver Nanoparticles ◽

Oral Biofilms ◽

Oral Microbes ◽

Average Size

Nanotechnology is a new discipline with huge applications including medicine and pharmacology industries. Although several methods and reducing agents have been employed to synthesize silver nanoparticles, reactive chemicals promote toxicity and nondesired effects on the human and biological systems. The objective of this work was to synthesize silver nanoparticles fromGlycyrrhiza glabraandAmphipterygium adstringensextracts and determine their bactericidal and antimycotic activities againstEnterococcus faecalisandCandida albicansgrowth, respectively. 1 and 10 mM silver nitrate were mixed with an extract ofGlycyrrhiza glabraandAmphipterygium adstringens.Green silver nanoparticles (AgNPs) were characterized by TEM, Vis-NIR, FTIR, fluorescence, DLS, TGA, and X-ray diffraction (XRD) analysis. Bactericidal and antimycotic activities of AgNPs were determined by Kirby and Bauer method and cell viability MTT assays. AgNPs showed a spherical shape and average size of 9 nm if prepared withGlycyrrhiza glabraextract and 3 nm if prepared withAmphipterygium adstringensextract. AgNPs inhibited the bacterial and fungal growth as was expected, without a significant cytotoxic effect on human epithelial cells. Altogether, these results strongly suggest that AgNPs could be an interesting option to control oral biofilms.

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Biomimetic Synthesis of Silver Nanoparticles from Aqueous Extract of Saraca indica and its Profound Antibacterial Activity

Biointerface Research in Applied Chemistry ◽

10.33263/briac111.81108120 ◽

2020 ◽

Vol 11 (1) ◽

pp. 8110-8120

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Aqueous Extract ◽

Silver Nanoparticle ◽

Pathogenic Bacteria ◽

Drug Formulation ◽

Biomimetic Synthesis ◽

Visible Absorption ◽

The Stability ◽

Average Size

The present findings were focused on green synthesis of silver nanoparticles through an aqueous extract of Saraca indica. The stability of the nanoparticle was achieved through the optimization of physico-chemical parameters. The sharp UV-visible absorption maximum at 400 was observed for biological synthesized silver nanoparticles. The spectroscopic analysis was thus used to assess the formation of silver nanoparticles. The AFM analysis did analyze the morphology of the nanocomposite, which was further confirmed through TEM micrograph. The electron micrograph image discloses that silver nanoparticles were polydispersed and dominantly as spherical with size ranges from 40nm to 100nm. The average size distribution was 49nm. The chemical reductions of Ag+ ions were further confirmed through FTIR. The biogenic silver nanoparticle and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The flavonoids rich binding of silver nanoparticle showed great medicinal potential and can be used for the treatment of several harmful infectious diseases. Hence, plant-based metal nanoparticles meet the demand for less toxic formulation during drug development and its delivery.

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Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

10.21203/rs.3.rs-551191/v1 ◽

2021 ◽

Author(s):

Susmila Aparna Gaddam ◽

Venkata Subbaiah Kotakadi ◽

Gunasekhar. Kalavakunta ◽

Josthna Penchalaneni ◽

Varadarajulu Naidu Challagundla ◽

...

Keyword(s):

Silver Nanoparticles ◽

Radical Scavenging ◽

Human Colon ◽

Xrd Analysis ◽

Antimicrobial Activities ◽

Human Colon Cancer ◽

Tem Analysis ◽

Insectivorous Plant ◽

Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

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Large-Scale Synthesis of Polymer-Stabilized Silver Nanoparticles

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.124-126.1189 ◽

2007 ◽

Vol 124-126 ◽

pp. 1189-1192 ◽

Cited By ~ 11

Author(s):

Kwi Jong Lee ◽

Young Il Lee ◽

In Keun Shim ◽

Byung Ho Jun ◽

Hye Jin Cho ◽

...

Keyword(s):

Silver Nanoparticles ◽

Large Scale ◽

Spherical Shape ◽

Acid Group ◽

Vinyl Pyrrolidone ◽

Molar Ratio ◽

Reaction Yield ◽

Ag Nps ◽

Average Size ◽

Polymer Stabilized

The synthesis and characterization of polymer-stabilized silver nanoparticles (Ag NPs) for water-based silver inks are studied. In order to synthesize Ag NPs with spherical shape, the conventional polyol processes require an excess of poly(vinyl pyrrolidone) (PVP) (10 ~ 1000 times than AgNO3) and therefore result in low productivity per reactor volume. In this study, poly(acrylic acid) (PAA) with carboxylic acid group was used instead of PVP. Even at less molar ratio of PAA to AgNO3 (< 2), the spherical Ag NPs with average size of 30 nm were successfully synthesized at 100 gram-scale with high reaction yield of 90%. Furthermore, the Ag NPs were dispersed into alcohol-based solvent and then patterned into metallic lines through inkjet printing technology.

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Biological Synthesis of Silver Nanoparticles by Cell-Free Extract ofSpirulina platensis

Journal of Nanotechnology ◽

10.1155/2015/132675 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 16

Author(s):

Gaurav Sharma ◽

Nakuleshwar Dut Jasuja ◽

Manoj Kumar ◽

Mohammad Irfan Ali

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Large Scale ◽

Cost Effective ◽

Biological Synthesis ◽

Cell Free Extract ◽

Tem Analysis ◽

High Antibacterial Activity ◽

Vis Spectroscopy ◽

Average Size

The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.

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