scholarly journals Immunological studies of mouse decidual cells. I. Membrane markers of decidual cells in the days after implantation.

1978 ◽  
Vol 148 (2) ◽  
pp. 580-591 ◽  
Author(s):  
O Bernard ◽  
M P Scheid ◽  
M A Ripoche ◽  
D Bennett
Keyword(s):  
B Lymphocytes ◽  
Hormonal Control ◽  
Decidual Cell ◽  
Uterine Tissue ◽  
Trypsin Treatment ◽  
Membrane Markers ◽  
Decidual Cells ◽  
Surface Immunoglobulins ◽  
Blocking Antibodies ◽  
Number Of Cells

Mouse decidual cell suspensions from day 6 to day 8 of gestation were prepared by enzymatic treatment with collagenase and trypsin and tested for various membrane markers. (a) Besides H-2 antigens, Thy-1 antigens are present on about 50% of the cells; this may reflect the fibroblastic origin of decidual cells or be a marker expressed on some decidual cells possibly under hormonal control. (b) T or B lymphocytes, as defined by four Lyt antigens or surface immunoglobulins, are not present in significant amounts. (c) A substantial number of cells bearing receptors for the Fc portion of IgG (FcR) is detectable in the decidua, probably closely connected with trophoblast cells; these FcR-bearing cells may act in preventing excessive invasion of uterine tissue by trophoblast or could contribute to the protection of the embryo by interacting with maternal blocking antibodies and trophoblast. No receptors for for complement were detected, even after 16-20 h in culture after trypsin treatment.

Renal transplantation: HLA-linked, non-cytotoxic antibodies develop after blood transfusion

1988 ◽  
Vol 74 (4) ◽  
pp. 385-388 ◽  
Author(s):  
Alison M. MacLeod ◽  
Keith N. Stewart ◽  
Stanislaw Urbaniak ◽  
Graeme R. D. Catto
Keyword(s):  
Blood Transfusion ◽  
B Lymphocytes ◽  
Blood Donor ◽  
Hla Antigens ◽  
Fc Receptor ◽  
Antibody Activity ◽  
Receptor Blocking ◽  
Single Donor ◽  
Before And After ◽  
Blocking Antibodies

1. A previously untransfused dialysis patient was given blood from a single donor on three occasions and sera were obtained from the recipient before and after transfusion. 2. The sera were tested against B-lymphocytes from the blood donor and his family in the erythrocyte–antibody rosette inhibition assay to determine whether any Fc-receptor-blocking activity which developed was HLA linked. 3. Antibody activity was noted after the second transfusion and was directed toward B-lymphocytes from the blood donor and his family members sharing the haplotype HLA A11, B12 with him. 4. These antibodies were removed by the absorption of active sera with lymphocytes from the blood donor but not from (a) his sibling who shared no HLA antigens with him or (b) the transfusion recipient. Absorption of the active sera with platelets from the blood donor did not remove activity. 5. These results indicate that Fc-receptor-blocking antibodies developing after blood transfusion are directed to HLA-linked antigens on donor lymphocytes.

scholarly journals Reflexive gap junctions. Gap junctions between processing arising from the same ovarian decidual cell.

1976 ◽  
Vol 69 (2) ◽  
pp. 495-501 ◽  
Author(s):  
J C Herr
Keyword(s):  
Electron Microscopy ◽  
Gap Junctions ◽  
Gap Junction ◽  
Decidual Cell ◽  
Hexagonal Array ◽  
Decidual Cells

Ajacent processes on ovarian decidual cells were shown by electron microscopy to form gap junctions with one another. Micrographs of tissues preserved with lanthanum included in the fixative confirm the hexagonal array and 2-4 nm gap which characterize gap junctions. It is suggested that these gap junctions may play a role in the process of merocrine secretion from the peduncular processes of ovarian decidual cells. The term reflexive gap junction is introduced to describe gap junctions between adjacent processes from the same cell.

scholarly journals The VP6 Protein of Rotavirus Interacts with a Large Fraction of Human Naive B Cells via Surface Immunoglobulins

2004 ◽  
Vol 78 (22) ◽  
pp. 12489-12496 ◽  
Author(s):  
Nathalie Parez ◽  
Antoine Garbarg-Chenon ◽  
Cynthia Fourgeux ◽  
Françoise Le Deist ◽  
Annabelle Servant-Delmas ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cells ◽  
B Lymphocytes ◽  
B Lymphocyte ◽  
Large Fraction ◽  
Memory Cell ◽  
Viral Gastroenteritis ◽  
Human B Cells ◽  
Surface Immunoglobulins ◽  
The Individual

ABSTRACT Immunity to human group A rotavirus (RV), a major cause of viral gastroenteritis in infants, involves B lymphocytes that provide RV-specific antibodies. Additionally, some arguments suggest that naive B cells could be implicated in the first steps of the immune response against RV. The aim of our study was to analyze the interaction of VP6 and VP7 RV capsid proteins with human B cells depending on the immune status of the individual, i.e., naive or RV experienced. For this purpose, a two-color virus-like particle flow cytometry assay was devised to evaluate the blood B-lymphocyte reactivity to VP6 and VP7 proteins from healthy RV-exposed adults, recently infected infants, and neonates at birth. Both VP6 and VP7 interactions with B cells were mediated by surface immunoglobulins and probably by their Fab portions. VP7-reactive B lymphocytes were mainly detected from RV-experienced patients and almost exclusively in the CD27-positive memory cell fraction. Conversely, VP6-reactive B lymphocytes were detected at similar and high frequencies in adult, infant, and neonate samples. In adult samples, VP6 reacted with about 2% of the CD27-negative (CD27neg) naive B cells. These results demonstrated that the VP6 RV protein interacted with a large fraction of naive B lymphocytes from both adults and neonates. We propose that naive B cell-VP6 interaction might influence the strength and quality of the acquired immune response and should be considered for elaborating RV vaccine strategies.

scholarly journals Bone marrow origin of decidual cell precursors in the pseudopregnant mouse uterus.

1982 ◽  
Vol 155 (5) ◽  
pp. 1537-1554 ◽  
Author(s):  
M Kearns ◽  
P K Lala
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Protein A ◽  
Specific Marker ◽  
Decidual Cell ◽  
Splenic Lymphocytes ◽  
Mouse Uterus ◽  
Endometrial Stromal Cells ◽  
Decidual Cells ◽  
Bone Marrow Origin

Decidual cells are considered to be the endproduct of a hormonally induced transformation of endometrial stromal cells of the uterus. However, the source of these precursors remains unknown. This study of evaluated the possibility of their bone marrow origin by an examination of the H-2 phenotype of decidual cells in pseudopregnant bone marrow chimeras. These chimeras were produced by repopulating lethally irradiated CBA/J female (H-2k) mice with bone marrow from (CBA/J x C57BL/6J) F1 female (H-2kb) mice. Pseudopregnancy was produced with a hormonal regimen followed by an oil-induced decidual stimulus. Chimerism was evaluated radioautographically by an identification of the donor-specific Kb phenotype on cells with an immunolabeling technique with monospecific anti-H-2 serum followed by radioiodinated protein A. The extent of chimerism as indicated by the degree of Kb labeling on decidual cells as well as macrophages contained within the decidual nodules was quantitatively compared with that seen on splenic lymphocytes. Fair to good chimerism, as reflected by labeling for the donor-specific marker (Kb), was seen on splenic lymphocytes and macrophages within the decidual nodules in 6 out of 11 animals. A similar level of chimerism was detected on decidual cells in all but one of these six, in which case this was low. One animal showed low chimerism in the spleen but good chimerism on the decidual cells. The remaining four mice were nonchimeric for all three cell types. These results indicate that decidual cells and macrophages appearing within the decidual nodules of pseudopregnant mice are ultimate descendants of bone marrow cells.

The relationship between decidualization and prolactin mRNA and production at different stages of human pregnancy

1995 ◽  
Vol 14 (2) ◽  
pp. 255-261 ◽  
Author(s):  
W-X Wu ◽  
J Brooks ◽  
A F Glasier ◽  
A S McNeilly
Keyword(s):  
Gene Expression ◽  
Amniotic Fluid ◽  
Stromal Cells ◽  
Decidual Cell ◽  
Close Link ◽  
Human Pregnancy ◽  
Decidual Cells ◽  
Prolactin Gene ◽  
Decidual Prolactin ◽  
Prolactin Mrna

ABSTRACT Within the human utero-placental unit only decidualized stromal cells express mRNA for prolactin. However, it is not clear if the level of prolactin production is related to the number of decidualized cells or the capacity of individual decidual cells to synthesize prolactin, either or both of which parameters may change during pregnancy. In the present study, prolactin production at different stages of human pregnancy was examined using quantitative in situ hybridization to assess decidual prolactin mRNA abundance, immunocytochemistry to examine the prolactin content inside decidual cells and RIA to measure decidual prolactin output into amniotic fluid. Throughout pregnancy the proportion of stromal cells showing positive immunostaining and mRNA for prolactin increased. There was a parallel increase in decidual cell size which was correlated with an increase in prolactin gene expression and intensity of immuno-staining for prolactin in individual decidual cells. These changes in decidual cells were consistent with the changes in the concentration of prolactin in amniotic fluid. These results suggest that there is a close link between the level of prolactin gene expression and production of prolactin by individual decidual cells, which in turn is directly related to the process of decidualization that continues throughout human pregnancy.

scholarly journals Morphological and immunofenotypic features of the monoclonal population of B-lymphocytes in chronic lymphocytic leukemia

2020 ◽  
Vol 1 (3) ◽  
pp. 27-35
Author(s):  
N. K. Guskova ◽  
O. N. Selyutina ◽  
I. A. Novikova ◽  
A. Yu. Maksimov ◽  
A. S. Nozdricheva ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Light Scattering ◽  
Chronic Lymphocytic Leukemia ◽  
B Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Light Chains ◽  
Group I ◽  
Surface Immunoglobulins ◽  
Group Ii ◽  
Morphological Differences

Purpose of the study. To evaluate the features of morphological and immunophenotypic characteristics of the lymphoid population with different restriction of light chains of immunoglobulins in patients with chronic lymphocytic leukemia (CLL).Materials and methods. The study included 30 CLL patients aged 47–79 years (20 men and 10 women). All patients underwent a General clinical blood test (SysmexXE 2100, Japan), morphological examination of the bone marrow (BioVision; Micros, Austria), immunophenotyping of bone marrow and peripheral blood by flow cytofluorometry (Navios10/3, Beckman Coulter, USA). B-cell clonality established by detection of restriction of light chains of surface immunoglobulins kappa or lambda. Morphological analysis of lymphocytes that differ in the expression of light chains of surface immunoglobulins: kappa (k) — group I (22 people — 73,3%), lambda (λ) — group II (8 people — 26,7%).Results. Determination of cell types by values of direct (FSC) and lateral (SSC) light scattering during immunophenotyping of peripheral blood and bone marrow samples showed that in patients of group I (CD19k+/CD5+/CD23+) on the light scattering diagram, the lymphoid population had low parameters: on the FSC scale — from 200 to 400, on the SSC — from 10 to 160 units, which indicates morphological uniformity of cells. In group II (CD19λ+/CD5+/CD23+), on the contrary, on the light scattering sketogram, the lymphoid zone was heterogeneous and stretched: on the FSC scale — from 200 to 1000, on the SSC — from 10 to 400 units, which indicates morphological polymorphism of cells. There were also differences in the expression of the common leukocyte antigen CD45. In group I, the expression is higher: the population of B-lymphocytes in terms of fluorescence intensity is on the dot graph on the CD45 scale in the second half of the third decade and in the fourth decade — to the right, than in group II, in which B-lymphocytes lie in the third decade. The data indicate that the CD19k+/CD5+/CD23+ population is represented by Mature cells, while the Cd19k+/CD5+/CD23+ population is represented by less Mature and / or intermediate forms. Significant morphological differences in lymphocyte populations were also observed in microscopic studies of blood and bone marrow preparations.Conclusion. The established immunophenotypic and morphological differences in lymphoid populations expressing either kappa — or lambda-light chains of immunoglobulins may be important for identifying risk groups among patients with biologically heterogeneous variants of chronic lymphocytic leukemia.

scholarly journals Decidual cell FKBP51–progesterone receptor binding mediates maternal stress–induced preterm birth

2021 ◽  
Vol 118 (11) ◽  
pp. e2010282118
Author(s):  
Ozlem Guzeloglu-Kayisli ◽  
Nihan Semerci ◽  
Xiaofang Guo ◽  
Kellie Larsen ◽  
Asli Ozmen ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Stress Disorder ◽  
Maternal Stress ◽  
Oxytocin Receptor ◽  
Decidual Cell ◽  
Fk506 Binding Protein ◽  
Decidual Cells ◽  
Human Labor ◽  
The Brain

Depression and posttraumatic stress disorder increase the risk of idiopathic preterm birth (iPTB); however, the exact molecular mechanism is unknown. Depression and stress-related disorders are linked to increased FK506-binding protein 51 (FKBP51) expression levels in the brain and/or FKBP5 gene polymorphisms. Fkbp5-deficient (Fkbp5−/−) mice resist stress-induced depressive and anxiety-like behaviors. FKBP51 binding to progesterone (P4) receptors (PRs) inhibits PR function. Moreover, reduced PR activity and/or expression stimulates human labor. We report enhanced in situ FKBP51 expression and increased nuclear FKBP51-PR binding in decidual cells of women with iPTB versus gestational age-matched controls. In Fkbp5+/+ mice, maternal restraint stress did not accelerate systemic P4 withdrawal but increased Fkbp5, decreased PR, and elevated AKR1C18 expression in uteri at E17.25 followed by reduced P4 levels and increased oxytocin receptor (Oxtr) expression at 18.25 in uteri resulting in PTB. These changes correlate with inhibition of uterine PR function by maternal stress–induced FKBP51. In contrast, Fkbp5−/− mice exhibit prolonged gestation and are completely resistant to maternal stress–induced PTB and labor-inducing uterine changes detected in stressed Fkbp5+/+ mice. Collectively, these results uncover a functional P4 withdrawal mechanism mediated by maternal stress–induced enhanced uterine FKBP51 expression and FKPB51-PR binding, resulting in iPTB.

scholarly journals Characterization by immunoperoxidase of the lymphocytes with bundle- shaped tubular (BST) inclusions in human normal peripheral blood

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 67-70 ◽  
Author(s):  
MD Appay ◽  
J Bariety ◽  
R Bretton
Keyword(s):  
T Cells ◽  
B Cells ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Normal Subjects ◽  
Lymphocyte Population ◽  
T And B Cells ◽  
Normal Peripheral Blood ◽  
Human Immunoglobulins ◽  
Surface Immunoglobulins

Abstract In six normal subjects, no surface immunoglobulins were detected on blood lymphocytes containing bundle-shaped tubular (BST) inclusions, after incubation with anti-human IgM peroxidase-labeled Fab'2 fragments. These Fab'2 fragments from pepsin-digested sheep anti-human immunoglobulins revealed human mu kappa, and lambda chains. These results suggest that the BST inclusions do not belong to B lymphocytes but belong to T cells and/or a third lymphocyte population, including K cells and precursors of T and B cells.

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