scholarly journals Two subsets of human T lymphocytes expressing gamma/delta antigen receptor are identifiable by monoclonal antibodies directed to two distinct molecular forms of the receptor.

1988 ◽  
Vol 168 (2) ◽  
pp. 491-505 ◽  
Author(s):  
C Bottino ◽  
G Tambussi ◽  
S Ferrini ◽  
E Ciccone ◽  
P Varese ◽  
...  
Keyword(s):  
Target Cell ◽  
Peripheral Blood ◽  
Minor Component ◽  
The Other ◽  
Molecular Forms ◽  
Gamma Delta ◽  
Gamma Receptor ◽  
Other Hand ◽  
Reducing Conditions ◽  
A Minor

Two mAbs directed to the TCR-gamma/delta were analyzed for their pattern of reactivity with CD3+WT31- cell populations or clones. In normal individuals, the BB3 mAb reacted with approximately 2/3 of peripheral blood CD3+WT31- lymphocytes, whereas delta-TCS-1 stained approximately 1/3 of such cells. In addition, the sum of the percentages of BB3+ and delta-TCS-1+ cells approximated the percentages of peripheral blood CD3+WT31- lymphocytes in seven normal donors tested. Also, in peripheral blood-derived polyclonal CD3+WT31- populations, cultured in IL-2, cells reacting with one or another mAb accounted for the whole cell population. On the other hand, only delta-TCS-1-reactive cells, but not BB3+ cells, could be detected in unfractionated as well as in CD4-8-thymocyte populations. Analysis of peripheral blood-derived CD3+WT31- clones showed that 70% of 72 clones analyzed reacted with BB3 mAb, but not with delta-TCS-1 mAb. On the other hand, delta-TCS-1 mAb stained the remaining BB3- clones. Five clones expressing medium-low amounts of CD8 antigen were BB3- delta-TCS-1+. Both types of clones lysed the Fc gamma receptor-bearing P815 target cell in the presence of anti-CD3 mAb (but not of mAb directed against HLA-DR, CD7 molecules, or TCR-alpha/beta). In this cytolytic assay, BB3 mAb induced target cell lysis only by BB3+ clones, whereas delta-TCS-1 mAb was effective only with delta-TCS-1+ clones. The CD3-associated surface molecules expressed by BB3+ or delta-TCS-1+ clones were analyzed after cell surface iodination and immunoprecipitation with the corresponding anti-TCR mAb or with anti-CD3 mAb (in digitonin-containing buffer). In SDS-PAGE, molecules immunoprecipitated from 13 BB3+ clones displayed, under nonreducing conditions, a molecular weight of 80 kD (in some cases, a minor 38-kD band could be detected). Under reducing conditions, two major components of 44 and 41 kD (and a minor component of 38 kD) were detected. On the other hand, TCR molecules immunoprecipitated from 11 different delta-TCS-1+ clones appeared as a diffuse band of 41-44 kD, both under reducing and nonreducing conditions (under non-reducing condition, an additional 38-kD band was present). Therefore, BB3+ cells express a disulphide-linked form of TCR-gamma/delta whereas delta-TCS-1+ cells express a non-disulphide-linked form.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Murine B-cell subpopulations responsive to T-dependent and T-independent antigens.

1976 ◽  
Vol 144 (2) ◽  
pp. 382-397 ◽  
Author(s):  
G K Lewis ◽  
R Ranken ◽  
D E Nitecki ◽  
J W Goodman
Keyword(s):  
B Cells ◽  
B Cell ◽  
Minor Component ◽  
Memory Cell ◽  
Keyhole Limpet Hemocyanin ◽  
The Other ◽  
Other Hand ◽  
Cell Mitogen ◽  
A Minor

Strain A/J mice made secondary indirect plaque-forming cell (PFC) responses to azobenzenearsonate (ABA) conjugates of giant keyhole limpet hemocyanin (KLH), a thymic-dependent antigen, but not to conjugates of Ficoll, a T-independent antigen. ABA-Ficoll was also unable to elicit a response in animals primed with ABA-KLH, which have an expanded anti-ABA memory cell pool. On the other hand, ABA-Ficoll rendered mice unresponsive to ABA-KLH when administered before priming or boosting with the T-dependent immunogen. Hence, the T-independent antigen was able to tolerize but unable to trigger B-memory cells responsive to the T-dependent antigen. A/J mice immunized with dinitrophenyl conjugates of Ficoll or bovine IgG (BGG) made vigorous IgM and IgG PFC responses. PFC responses to ABA-KLH and 2,4-dinitrophenyl (DNP)-BGG were abrogated by depleting mice of C3 with cobra venom factor, whereas the IgM and IgG PFC responses to DNP-Ficoll were unaffected. B lymphocytes were fractionated on the basis of receptors for C3 and the subpopulations were assayed for in vitro PFC responses to DNP-Ficoll. Very little response was obtained from complement receptor lymphocyte [CRL(+)] B cells, whereas CRL(-) cells were more responsive than unfractionated B cells. Both populations responded to a polyclonal B-cell mitogen (lipopolysaccharide). On the other hand, the in vitro PFC response to a T-dependent antigen (sheep erythrocytes) correlated with the presence of CRL(+) B cells in the cultures. However, a minor component of this response, sensitive to anti-Thy-1 serum, was made by CRL(-) B cells, indicating the existence of subpopulations of T-dependent B cells with different signalling requirements. The results suggest that most B cells responsive to T-dependent antigens possess receptors for C3 and that C3 plays an obligatory role in the response of these cells. A distinct subpopulation of B cells which lack C3 receptors respond to T-independent antigens. The precursors of PFC for the ABA epitope reside largely or exclusively in the CRL(+) compartment in A/J mice, whereas precursors for the DNP determinant are found in both compartments.

CORTICOTROPIN-BESTIMMUNG ANHAND DES CORTICOSTERONANSTIEGES IM NEBENNIEREN-VENENBLUT HYPOPHYSEKTOMIERTER RATTEN

1962 ◽  
Vol 41 (2) ◽  
pp. 211-218 ◽  
Author(s):  
K. Retiene ◽  
H. Ditschuneit ◽  
M. Fischer ◽  
K. Kopp ◽  
E. F. Pfeiffer
Keyword(s):  
Peripheral Blood ◽  
Single Injection ◽  
Venous Blood ◽  
Corticosterone Level ◽  
The Other ◽  
Adrenal Vein ◽  
Major Surgery ◽  
Other Hand ◽  
Hypophysectomized Rats

ABSTRACT Corticotrophin has been measured by using the corticotrophin-induced increase of corticosterone in adrenal venous blood of rats, the corticotrophin secretion of which has been blocked by preliminary injection of dexamethasone. Sensitivity and precision of this technique have not been higher than in the simpler procedure using corticosterone increase in peripheral blood. Single injection of dexamethasone on the other hand did not prevent release of endogenous corticotrophin following major surgery, required for canulation of the adrenal vein. In hypophysectomized rats corticotrophin can be measured by using adrenal venous blood. 0.05 mU corticotrophin (US-P-Standard) has been determined with an index of precision of λ = 0.13. The consistent relation between initial and elevated corticosterone level following corticotrophin in both peripheral and adrenal venous blood makes it highly unlikely that other modifications of this kind of assay will increase sensitivity.

A Medieval Open Work

POETICA ◽  
2021 ◽  
Vol 52 (3-4) ◽  
pp. 228-265
Author(s):  
Rafael Simian
Keyword(s):  
The Other ◽  
Specific Function ◽  
Umberto Eco ◽  
New Approach ◽  
Avant Garde ◽  
Other Hand ◽  
Open Work ◽  
New Perspective ◽  
A Minor

Abstract Guigo II is commonly known and praised among specialists of Western mysticism for his Scala claustralium, a work that presents a spiritual program for cloistered monks. His Meditations, on the other hand, have usually been relegated to the margin of attention. The First Meditation, in particular, is generally regarded as a minor piece. The paper argues, however, that a new approach can make better sense of the First Meditation, while also enabling us to recognize its specific function and value. Seen from this new perspective, Guigo’s purpose with the text is to train and exercise his readers’ minds according to the spiritual program laid out in the Scala. The paper shows that the First Meditation realizes that goal, surprisingly, by having the same essential features that Umberto Eco found in the ‘open works’ of the Western avant-garde.

scholarly journals Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 876-884 ◽  
Author(s):  
J Cashman ◽  
D Henkelman ◽  
K Humphries ◽  
C Eaves ◽  
A Eaves
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Small Volume ◽  
Early Stage ◽  
Final Concentration ◽  
The Other ◽  
Normal Individuals ◽  
Additional Control ◽  
Minor Population ◽  
A Minor

Abstract Erythropoietic progenitors from peripheral blood of normal individuals or patients with polycythemia vera (PV) were cultured in methylcellulose medium containing 2.5 U/ml of erythropoietin (Ep). After 7–9 days, colonies considered to be early stage large bursts were individually removed, resuspended in a small volume of fresh methylcellulose medium, and then divided between 2 dishes. To one of these secondary cultures, sufficient Ep was added to bring the concentration of Ep up to approximately 3 U/ml. To the other was added an equal volume of medium but no Ep. The final concentration of Ep in these cultures was determined to be less than 0.01 U/ml. Nine days later, both types of secondary cultures were scored for the presence of colonies containing 8 or more hemoglobinized erythroblasts. Of 90 primary colonies from 3 normal individuals assessed in this way, 59 gave secondary erythroid colonies in the high Ep cultures, while none gave secondary erythroid colonies in the low Ep cultures. Additional control experiments in which primary colonies from normal individuals were divided into duplicate high Ep cultures showed that on average, the procedure used divided primary colonies equally. Of 109 primary colonies from 5 PV patients that yielded secondary erythroid colonies in the high Ep cultures, 21 yielded no secondary erythroid colonies in the low Ep cultures. The other 88 yielded erythroid colonies in both, but the secondary colonies in the low Ep cultures were consistently smaller in size and significantly fewer in number. Similar results were obtained when primary colonies were generated in cultures to which no Ep was added. These findings indicate that primitive BFU-E in patients with PV can be subdivided into 2 populations: a minor population restricted to the production of erythroid colony-forming cells (Ep- dependent progenitors) that require Ep for their detection, and a major population that is not restricted in this way. In addition, these experiments show that most of the primitive BFU-E that generate Ep- independent progenitors also produce significant numbers of cells that are Ep-dependent.

scholarly journals Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.

1988 ◽  
Vol 167 (5) ◽  
pp. 1625-1644 ◽  
Author(s):  
J Borst ◽  
J J van Dongen ◽  
R L Bolhuis ◽  
P J Peters ◽  
D A Hafler ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Cell Lineage ◽  
Cell Receptor ◽  
Cytolytic Activity ◽  
Molecular Forms ◽  
Gamma Delta ◽  
Gamma Chain ◽  
Human T Cell

A second type of TCR molecule has been identified on human and murine T lymphocytes, which involves the protein products of the gamma and delta genes. T lymphocytes bearing this receptor may constitute a separate cell lineage with a distinct immune function. We have produced an mAb, which specifically detects human TCR-gamma/delta in native as well as denatured states, this in contrast to previously used anti-gamma chain peptide sera, which only reacted with denatured protein. The receptor occurs in different molecular forms, with or without interchain disulphide bonds, in which a delta chain may or may not be detected by cell surface iodination. The mAb is reactive with all these receptor forms. Therefore, this antibody could be used to determine the expression of TCR-gamma/delta on viable human T lymphocytes. In normal individuals, TCR-gamma/delta was found on a subset composing 2-7% of CD3+ lymphocytes in peripheral blood and 0.1-1.0% in thymus. The majority of these cells do not express the CD4 or CD8 antigens, although a significant percentage of CD8+ cells was found. TCR-gamma/delta+ cells in peripheral blood are resting lymphocytes, as judged by ultrastructural analysis. T cell clones with different receptor types can display MHC-nonrestricted cytolytic activity, which is shown to be induced by the culture conditions, most likely by growth factors such as IL-2. This strongly suggests that TCR-gamma/delta does not play a role in target cell recognition in MHC-nonrestricted cytotoxicity. The anti-TCR-gamma/delta antibody can specifically induce cytotoxic activity in clones expressing the receptor, but in addition inhibit growth factor induced cytotoxicity, which indicates a regulatory role of the TCR-gamma/delta/CD3 complex in MHC-nonrestricted cytotoxicity.

scholarly journals La reivindicación cultural e identitaria a través de la autotraducción: el caso de la poesía autotraducida de Xuan Bello del asturiano al español

2018 ◽  
Vol 17 (17) ◽  
pp. 171
Author(s):  
Beatriz Flores Silva
Keyword(s):  
The Other ◽  
Other Hand ◽  
The One ◽  
A Minor ◽  
Sociocultural Systems ◽  
Sobre Todo

La autotraducción es un fenómeno que siempre ha estado presente, sobre todo, en aquellos sistemas socioculturales de gran riqueza lingüística y cultural. Este es el caso del sistema peninsular, donde cada vez más autores bilingües deciden escribir sus obras en una lengua minoritaria y, después, autotraducirlas al español. En este trabajo, se toma como objeto de análisis la poesía autotraducida del asturiano al español de Xuan Bello. Así, se observará si la autotraducción permite a este autor traductor, por un lado, trasladar sus reivindicaciones culturales a otro sistema ajeno al asturiano; y, por otro lado, definir su identidad individual.Palabras clave: autotraducción, poesía, asturiano, español, cultura, identidad.Self-translation is a phenomenon which has always been present, most of all in those sociocultural systems characterized by both their linguistic and cultural richness. One of those is the Spanish peninsular system where more and more bilingual authors tend to write their works in a minor language and subsequently translate them into Spanish. The aim of this paper is to research Xuan Bello’s self-translated poetry from Asturian into Spanish. That way it will be considered whether selftranslation helps this author-translator: on the one hand, to transfer his cultural demands; on the other hand, to define his individual identity.Keywords: self-translation, poetry, Asturian, Spanish, culture, identity.

scholarly journals Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 876-884 ◽  
Author(s):  
J Cashman ◽  
D Henkelman ◽  
K Humphries ◽  
C Eaves ◽  
A Eaves
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Small Volume ◽  
Early Stage ◽  
Final Concentration ◽  
The Other ◽  
Normal Individuals ◽  
Additional Control ◽  
Minor Population ◽  
A Minor

Erythropoietic progenitors from peripheral blood of normal individuals or patients with polycythemia vera (PV) were cultured in methylcellulose medium containing 2.5 U/ml of erythropoietin (Ep). After 7–9 days, colonies considered to be early stage large bursts were individually removed, resuspended in a small volume of fresh methylcellulose medium, and then divided between 2 dishes. To one of these secondary cultures, sufficient Ep was added to bring the concentration of Ep up to approximately 3 U/ml. To the other was added an equal volume of medium but no Ep. The final concentration of Ep in these cultures was determined to be less than 0.01 U/ml. Nine days later, both types of secondary cultures were scored for the presence of colonies containing 8 or more hemoglobinized erythroblasts. Of 90 primary colonies from 3 normal individuals assessed in this way, 59 gave secondary erythroid colonies in the high Ep cultures, while none gave secondary erythroid colonies in the low Ep cultures. Additional control experiments in which primary colonies from normal individuals were divided into duplicate high Ep cultures showed that on average, the procedure used divided primary colonies equally. Of 109 primary colonies from 5 PV patients that yielded secondary erythroid colonies in the high Ep cultures, 21 yielded no secondary erythroid colonies in the low Ep cultures. The other 88 yielded erythroid colonies in both, but the secondary colonies in the low Ep cultures were consistently smaller in size and significantly fewer in number. Similar results were obtained when primary colonies were generated in cultures to which no Ep was added. These findings indicate that primitive BFU-E in patients with PV can be subdivided into 2 populations: a minor population restricted to the production of erythroid colony-forming cells (Ep- dependent progenitors) that require Ep for their detection, and a major population that is not restricted in this way. In addition, these experiments show that most of the primitive BFU-E that generate Ep- independent progenitors also produce significant numbers of cells that are Ep-dependent.

scholarly journals Clause and predicative constituents in an Austronesian language: Lampung language

2020 ◽  
Vol 21 (2) ◽  
pp. 62-79
Author(s):  
Afrianto ◽  
Eva Tuckyta Sari Sujatna ◽  
Nani Darmayanti ◽  
Farida Ariyani ◽  
Jessamine Cooke-Plagwitz
Keyword(s):  
The Other ◽  
Sentence Structure ◽  
Austronesian Language ◽  
Nominal Phrase ◽  
Other Hand ◽  
The Subject ◽  
A Minor ◽  
Dependent Clause

AbstractThis research is conducted qualitatively and aimed at patterning and describing clause and sentence structure in Lampung language through the configuration of its constituents. Regarding the constituents, Lampung has two types of clause: minor and major clauses. A minor clause is indicated by only one constituent, which is commonly a subject, predicate or adjunct. Regarding its function, it can be classified as vocative, shown by exclamation (Wuy!, Huy!); a greeting, as shown by an expression (tabikpun ngalam pukha); and an Arabic greeting (assalamualaikum). On the other hand, a major clause minimally consists of a subject and predicate, and apart from these there can also be an object, complement and adverbial. Furthermore, this research finds various categories that can act as predicative constituents: they are a verb/verbal phrase, adjective/adjective phrase, and noun/nominal phrase. Additionally, a copular verb (iyulah) and existential marker (wat) can also be the predicate. This research also reveals that in a sentence two or more clauses are connected by a conjunction, and then this conjunction becomes an indicator of dependent clauses. Also, a dependent clause can be found after the subject or the object of the independent clause.

Interferences appearing in fluorometrically measured liquid-chromatographic profiles of creatine kinase isoenzymes in serum.

1980 ◽  
Vol 26 (6) ◽  
pp. 707-711 ◽  
Author(s):  
T D Schlabach ◽  
J A Fulton ◽  
P B Mockridge ◽  
E C Toren
Keyword(s):  
Creatine Kinase ◽  
High Performance ◽  
Minor Component ◽  
The Other ◽  
Present Evidence ◽  
Human Sera ◽  
A Minor ◽  
Liquid Chromatographic ◽  
Measured Liquid ◽  
Chromatographic Profiles

Abstract We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by “high-performance” liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.

Heterogeneity of the prolactin receptor in the rat mammary gland and liver during various physiological states

1994 ◽  
Vol 141 (2) ◽  
pp. 271-278 ◽  
Author(s):  
P Guillaumot ◽  
H Cohen
Keyword(s):  
Mammary Gland ◽  
Rat Liver ◽  
Specific Binding ◽  
Prolactin Receptor ◽  
Ovariectomized Rats ◽  
Molecular Forms ◽  
Reducing Conditions ◽  
Binding Forms ◽  
Ovine Prolactin ◽  
A Minor

Abstract This work was undertaken to determine variations in the 125I-labelled ovine prolactin (oPRL) binding in rat liver and mammary gland membranes, and to study the molecular forms of prolactin receptor in different physiological situations. Prolactin binding was determined using 125I-labelled oPRL in the 100 000 g pellet. 125I-Labelled oPRL was cross-linked to receptors in membranes from rat liver and mammary gland and subjected to SDS-PAGE under reducing conditions, followed by autoradiography of dried gels. In the mammary gland, the specific binding of oPRL to membranes, expressed as mean ± s.e.m. fmol/mg protein increased from 1·36 ±0·24 on the day of dioestrus to 3·26 ±0·60 on the day of oestrus. It remained very low during pregnancy but increased during lactation to reach 4·78 ±0·99. In the liver, the specific binding of oPRL to membranes was higher than in the mammary gland on the days of dioestrus 1, dioestrus 2 and oestrus, but not on the day of pro-oestrus. It increased until day 14 of pregnancy when the specific binding of 125I-labelled oPRL was 17·01 ±0·30. Cross-linking revealed different molecular forms in the mammary glands and the liver. In the mammary gland we observed four prolactin-binding forms of 80, 50, 40 and 16 kDa, all of which were specific for prolactin. The 80, 50 and 40 kDa forms were also able to bind to a Concanavalin A–Sepharose column, indicating that these binding forms were glycosylated while the smaller one (16 kDa) appeared to be unglycosylated. The 40 kDa prolactin receptor was seen at all stages studied: the oestrous cycle (dioestrus, pro-oestrus and oestrus), pregnancy (days 8, 14 and 22) and lactation. The 50 kDa form was observed in the mammary gland during the day of pro-oestrus and gestation. It was also observed in ovariectomized rats treated with oestradiol (OE2), suggesting that OE2 could be one of the factors involved in the induction of this receptor form. The 16 kDa form of the receptor was found in the mammary gland only during lactation. This form, while unglycosylated, bound specifically to prolactin, suggesting that it might play a specific role during lactation. In the liver, two forms were shown, a major one of 40 kDa and a minor one of 80 kDa. No variation in receptor molecular weight was found in the liver during the physiological stages studied. Journal of Endocrinology (1994) 141, 271–278

Sign in / Sign up

Export Citation Format

Share Document