CORTICOTROPIN-BESTIMMUNG ANHAND DES CORTICOSTERONANSTIEGES IM NEBENNIEREN-VENENBLUT HYPOPHYSEKTOMIERTER RATTEN

1962 ◽  
Vol 41 (2) ◽  
pp. 211-218 ◽  
Author(s):  
K. Retiene ◽  
H. Ditschuneit ◽  
M. Fischer ◽  
K. Kopp ◽  
E. F. Pfeiffer
Keyword(s):  
Peripheral Blood ◽  
Single Injection ◽  
Venous Blood ◽  
Corticosterone Level ◽  
The Other ◽  
Adrenal Vein ◽  
Major Surgery ◽  
Other Hand ◽  
Hypophysectomized Rats

ABSTRACT Corticotrophin has been measured by using the corticotrophin-induced increase of corticosterone in adrenal venous blood of rats, the corticotrophin secretion of which has been blocked by preliminary injection of dexamethasone. Sensitivity and precision of this technique have not been higher than in the simpler procedure using corticosterone increase in peripheral blood. Single injection of dexamethasone on the other hand did not prevent release of endogenous corticotrophin following major surgery, required for canulation of the adrenal vein. In hypophysectomized rats corticotrophin can be measured by using adrenal venous blood. 0.05 mU corticotrophin (US-P-Standard) has been determined with an index of precision of λ = 0.13. The consistent relation between initial and elevated corticosterone level following corticotrophin in both peripheral and adrenal venous blood makes it highly unlikely that other modifications of this kind of assay will increase sensitivity.

scholarly journals γA-BLOOD GROUP ANTIBODIES

1966 ◽  
Vol 123 (5) ◽  
pp. 951-967 ◽  
Author(s):  
M. Adinolfi ◽  
P. L. Mollison ◽  
Margaret J. Polley ◽  
Jane M. Rose
Keyword(s):  
Blood Group ◽  
Single Injection ◽  
Deae Cellulose ◽  
Red Cells ◽  
The Other ◽  
Intravenous Injections ◽  
Other Hand

The serological characteristics of γA-anti-A and anti-B were studied using, as a source, either colostrum, or fractions relatively rich in γA obtained from selected potent antisera. γA-anti-A and anti-B were never hemolytic nor did they sensitize red cells to agglutination by anticomplement globulin sera. γA-anti-A, like γG-anti-A and unlike γM-anti-A was unaffected by heating at 56°C for 3 hr. On the other hand in the following three characteristics the behavior of γA fell between that of γG- or γM-anti-A: sensitivity to inactivation by 2-mercaptoethanol, ease of neutralization by A substance and degree of enhancement of agglutination in a medium of serum rather than saline. The agglutination produced by γA-anti-A was regularly enhanced by addition of anti-γA-globulin serum. In searching for γA-blood group antibodies of other specificities the following sera were tested: anti-D (32 examples); anti-c (2 examples); anti-Lea or -Leb (3 examples); anti-K (3 examples); anti-Fya (3 examples), and anti-Jka (3 examples). Only 3 sera, all containing anti-D, sensitized red cells to agglutination by anti-γA. There were no discrepancies between results obtained with four different anti-γA-globulin sera. Approximately half the sera were fractionated on DEAE-cellulose, and the fractions rich in γA tested for their ability to sensitize red cells to agglutination by anti-γA; no additional examples of γA-antibodies were detected. One of the three examples of γA-anti-D appeared in the serum of a woman during the course of deliberate reimmunization. γA-anti-D appeared only after three intravenous injections of red cells although the γG-anti-D titer rose considerably after a single injection. 3 yr after a fourth injection of Rh-positive cells γA-anti-D, as well as γG-anti-D, was still present in the serum.

Effect of growth hormone and thyroxine on growth and chemical composition of muscle, bone and other tissues in thyroidectomized-hypophysectomized rats

1959 ◽  
Vol 196 (4) ◽  
pp. 859-865 ◽  
Author(s):  
Robert O. Scow
Keyword(s):  
Growth Hormone ◽  
Chemical Composition ◽  
Growth Response ◽  
Pronounced Effect ◽  
The Other ◽  
Growth Hormone Administration ◽  
Hormone Administration ◽  
Other Hand ◽  
Hypophysectomized Rats ◽  
Accelerated Growth

Rats were thyroidectomized-hypophysectomized at weaning and 5 weeks later treated for 36 days with either growth hormone (0.1 or 0.5 mg/day), thyroxine (2.5 µg/day), or both. Growth hormone stimulated growth of all tissues whereas thyroxine accelerated growth of only muscle and certain viscera. The growth response of some tissues to thyroxine was as pronounced as that to growth hormone. Administration of thyroxine increased the response to growth hormone of only the tissues that grew when thyroxine was given alone. Although thyroxine had no effect on the amount of bone protein in growth hormone treated and untreated rats, it had a pronounced effect on the morphology (length and maturation) of bone. The amount of collagen in muscle increased with dosage of growth hormone; thyroxine had no effect on this response. Myosin, on the other hand, increased with growth hormone dosage only in those animals also given thyroxine. Thyroxine given alone increased myosin deposition in muscle but had no effect on collagen in muscle, bone or skin.

scholarly journals Two subsets of human T lymphocytes expressing gamma/delta antigen receptor are identifiable by monoclonal antibodies directed to two distinct molecular forms of the receptor.

1988 ◽  
Vol 168 (2) ◽  
pp. 491-505 ◽  
Author(s):  
C Bottino ◽  
G Tambussi ◽  
S Ferrini ◽  
E Ciccone ◽  
P Varese ◽  
...  
Keyword(s):  
Target Cell ◽  
Peripheral Blood ◽  
Minor Component ◽  
The Other ◽  
Molecular Forms ◽  
Gamma Delta ◽  
Gamma Receptor ◽  
Other Hand ◽  
Reducing Conditions ◽  
A Minor

Two mAbs directed to the TCR-gamma/delta were analyzed for their pattern of reactivity with CD3+WT31- cell populations or clones. In normal individuals, the BB3 mAb reacted with approximately 2/3 of peripheral blood CD3+WT31- lymphocytes, whereas delta-TCS-1 stained approximately 1/3 of such cells. In addition, the sum of the percentages of BB3+ and delta-TCS-1+ cells approximated the percentages of peripheral blood CD3+WT31- lymphocytes in seven normal donors tested. Also, in peripheral blood-derived polyclonal CD3+WT31- populations, cultured in IL-2, cells reacting with one or another mAb accounted for the whole cell population. On the other hand, only delta-TCS-1-reactive cells, but not BB3+ cells, could be detected in unfractionated as well as in CD4-8-thymocyte populations. Analysis of peripheral blood-derived CD3+WT31- clones showed that 70% of 72 clones analyzed reacted with BB3 mAb, but not with delta-TCS-1 mAb. On the other hand, delta-TCS-1 mAb stained the remaining BB3- clones. Five clones expressing medium-low amounts of CD8 antigen were BB3- delta-TCS-1+. Both types of clones lysed the Fc gamma receptor-bearing P815 target cell in the presence of anti-CD3 mAb (but not of mAb directed against HLA-DR, CD7 molecules, or TCR-alpha/beta). In this cytolytic assay, BB3 mAb induced target cell lysis only by BB3+ clones, whereas delta-TCS-1 mAb was effective only with delta-TCS-1+ clones. The CD3-associated surface molecules expressed by BB3+ or delta-TCS-1+ clones were analyzed after cell surface iodination and immunoprecipitation with the corresponding anti-TCR mAb or with anti-CD3 mAb (in digitonin-containing buffer). In SDS-PAGE, molecules immunoprecipitated from 13 BB3+ clones displayed, under nonreducing conditions, a molecular weight of 80 kD (in some cases, a minor 38-kD band could be detected). Under reducing conditions, two major components of 44 and 41 kD (and a minor component of 38 kD) were detected. On the other hand, TCR molecules immunoprecipitated from 11 different delta-TCS-1+ clones appeared as a diffuse band of 41-44 kD, both under reducing and nonreducing conditions (under non-reducing condition, an additional 38-kD band was present). Therefore, BB3+ cells express a disulphide-linked form of TCR-gamma/delta whereas delta-TCS-1+ cells express a non-disulphide-linked form.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Effects of Storage Duration and Temperature on the Values of Haematological Parameters in Bovine and Ovine Blood Samples

2018 ◽  
Vol 68 (2) ◽  
pp. 145
Author(s):  
A. H. KIRMIZIGUL ◽  
E. GOKCE ◽  
M. SOZMEN
Keyword(s):  
Venous Blood ◽  
Blood Parameters ◽  
The Other ◽  
Haematological Parameters ◽  
Bovine Blood ◽  
Storage Duration ◽  
Blood Samples ◽  
Study Material ◽  
Other Hand ◽  
Haematological Values

The purpose of the present study was to determine the alterations in haematological values at 0, 12, 24, 36, 48, 60, 72, 84 and 96 hours of storage in bovine and ovine venous blood samples stored at 4˚C and 24˚C in EDTA-coated tubes. Twenty healthy animals, including ten 4-year-old cattle and ten 2-year-old sheep constituted the study material. Bovine blood samples stored at 4˚C produced reliable results for the WBC, RBC, Hb, PCV, MCV, MCH and MCHC levels with the exception of PLT level as PLT levels decreased when the PLT stored 24 hours or longer times. On the other hand ovine blood samples stored at 4˚C for 24 hours or longer, all the parameters measured (WBC, RBC, Hb, PCV, MCV, MCH, MCHC and PLT levels) gave reliable results indicating that ovine blood parameters can be used effectively and safely. Furthermore, in the bovine blood samples, MCHC levels were decreased as from 60 hours stored at 24°C and WBC levels were decreased in the ovine blood samples stored 48 hours or longer at 24°C. However, the blood samples stored at 24°C, the measurement of the RBC, Hb, PCV, MCV, MCH and PLT levels produced reliable results.

SAT0119 PARADOXICAL NEUTROPHIL ACTIVATION BY ANTI-IL6 THERAPY: TRANSCRIPTOME ANALYSIS SHOWS A RATIONALE FOR DERMATOLOGICAL ADVERSE REACTIONS AND DECREASED NEUTROPHIL COUNTS AFTER TOCILIZUMAB TREATMENT

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 993.1-994
Author(s):  
M. Sakamoto ◽  
A. Senoh ◽  
Y. Sato ◽  
H. Iijima ◽  
M. Yamaguchi ◽  
...  
Keyword(s):  
Side Effects ◽  
Peripheral Blood ◽  
Side Effect ◽  
Neutrophil Migration ◽  
Neutrophil Activation ◽  
The Other ◽  
Leukocyte Migration ◽  
Other Hand ◽  
Serious Infections ◽  
Go Terms

Background:Skin rashes as a side effect of Tocilizumab therapy (TCZ- Tx) has not been paid much attention, because the incidence was only 1~2% in the drug information sheets. However, we experienced several RA cases with development of various skin rashes associated with neutrophil activation after TCZ-Tx. On the other hand, it is well known that the neutrophil counts in peripheral blood decreases after TCZ-Tx, whereas it does not affect the rate of serious infections. The detailed mechanism is still unclear.Objectives:To detect the characteristics of the changes in gene expressions of peripheral blood associated with TCZ-Tx and the development of skin rashes as its side effect.Methods:Total of 14 RA patients with TCZ-Tx were included. Among them, 4 patients developed TCZ-related rashes (group S) and 10 patients did not show any side effects (group C). Peripheral whole blood at just before (pre) and 3 months after (post) TCZ-Tx from each patient were subjected to the analysis. Total RNAs were extracted with PAXgene miRNA kit and analyzed with next-generation sequencing. First, group C was investigated for the normal response to TCZ-Tx. Differentially expressed genes (DEGs) were selected by paired comparison (post vs. pre). And then, enrichment analysis using gene ontology (GO) terms were performed. Second, to explore the characteristics of group S, all expressed genes in 14 cases at just before TCZ-Tx were subjected to a hierarchical clustering analysis. The DEGs (group S vs. C and post vs. pre) were also investigated with weighted gene co-expression network analysis (WGCNA) and GO analysis. Meanwhile, the total eigengene expressions of the important modules identified by WGCNA in each cases were also calculated.Results:Surprisingly, 8 out of the top 10 enriched GO terms in the up-regulated genes were relevant to leukocyte activation such as ‘neutrophil migration” by the analysis of DEGs (post vs. pre) in group C. The cluster analysis of ‘pre’ genes confirmed that the patterns of gene expression between group S and C was different. WGCNA analysis of DEGs (group S vs. C) revealed that genes related to acute inflammation such as ‘leukocyte mediated immunity’ were activated in group S. Interestingly, it was not correlated with disease activity score (DAS) of RA. By the analysis of DEGs (post vs. pre) of upregulated genes, we found that the total eigengene expressions of the module enriched with genes related to ‘cell adhesion’ or ‘leukocyte migration’ were significantly increased in all cases of group S.Conclusion:This is the first evidence that the genes associated with neutrophil migration is significantly activated after TCZ-Tx. It is noteworthy that the gene activation was observed in cases without any side effects. The decreased neutrophil counts in peripheral blood have been known after initiation of TCZ-Tx, which did not affect the rate of serious infections. Recently, It was reported that TCZ affects neutrophil trafficking to the bone marrow1). Our findings will provide a rationale for its cause. On the other hand, we experienced several RA cases with development of various skin rashes associated with neutrophil activation after TCZ-Tx. However, majority of patients do not develop the side effect, even though genes related to ‘neutrophil migration’ are activated. In group S, our findings indicate that the genes related to ‘leukocyte mediated immunity’ was already activated at the initiation of treatment without correlating to DAS of RA, furthermore, the gene upregulation related to ‘leukocyte migration’ was more prominent after TCZ-Tx. Although it is difficult to predict the patients developing skin rashes before TCZ-Tx, we do not recommend to use TCZ for the patients with neutrophilic dermatosis which is often associated with RA.References: :[1]Lok LSCet al.,Eur J Clin Invest. 47(10):736-745 (2017).Disclosure of Interests: :Moe Sakamoto: None declared, Akemi Senoh: None declared, Yoshiharu Sato: None declared, Hiroshi Iijima: None declared, Mari Yamaguchi: None declared, Toshie Higuchi: None declared, Yoshinobu Koyama Grant/research support from: Eli-Lilly and Mochida., Speakers bureau: BMS, Ayumi, Chugai, Ono, Mitsubishi Tanabe, Abbvie and Eisai.

EXCRETION PATTERNS OF 17-KETOSTEROIDS AND 17-HYDROXYCORTICOSTEROIDS IN SURGICAL STRESS

1970 ◽  
Vol 65 (1) ◽  
pp. 1-10 ◽  
Author(s):  
H. Tanaka ◽  
H. Manabe ◽  
K. Koshiyama ◽  
Y. Hamanaka ◽  
K. Matsumoto ◽  
...  
Keyword(s):  
Surgical Stress ◽  
The Other ◽  
Major Surgery ◽  
Marked Rise ◽  
Other Hand ◽  
Made In

ABSTRACT Quantitative determinations of urinary 17-ketosteroids (17-KS) and 17-hydroxycorticosteroids (17-OHCS) were made in 17 males and 8 females before and for 7 days following moderate to major surgery. The operation caused a marked rise in the excretion of total 17-OHCS. On the other hand, a significant decrease in the excretion of the total 17-KS was found following surgery. The postoperative decrease resulted from a significant decrease in the excretion of C19O2- 17-KS, since the urinary C19O3-17-KS remained almost unchanged following the operation. The postoperative decrease was shown to be of a higher degree in males since the decrease in the excretion of aetiocholanolone and androsterone was more marked in males than in females. Contrary to this, the excretion of both C19O3-17-KS and C19O2-17-KS increased following ACTH injection. From the result, it seems justifiable to assume that the secretion of androgen from the testes may decrease rapidly following operation. It was also demonstrated that the ratio of aetiocholanolone to androsterone increased postoperatively as did the ratio of THF to alloTHF. Furthermore, the ratios of THE to THF and E to F were found to be significantly decreased following surgery.

Ovarian Response to Exogenous Gonadotropins in Infant Cynomolgus Monkeys Hidenobu Sameshima

1996 ◽  
Vol 15 (3) ◽  
pp. 194-204 ◽  
Author(s):  
Satoru Oneda ◽  
Toshio Ihara ◽  
Katsumi Hamana
Keyword(s):  
Ovarian Function ◽  
Single Injection ◽  
Histopathological Examination ◽  
Ovarian Response ◽  
The Other ◽  
Human Menopausal Gonadotropin ◽  
Serum Estradiol ◽  
Cynomolgus Monkeys ◽  
Adult Females ◽  
Other Hand

The response of the ovaries to exogenous gonadotropins was examined by determining serum estradiol (E2) and progesterone (P) levels and by observing the development of follicles by using 5-to 7-month-old cynomolgus females. The serum E2 levels began to increase 2 h after a single injection of 15 IU of human menopausal gonadotropin (h MG) and reached peak levels at 6 h after dosing, but the E2levels decreased to near predosing values at 24 h. The serum E2 levels increased markedly due to the repeated injections of 15 IU h MG for 10 days (days 0-9), but the P levels were low and equal to the levels observed at the follicular stage in adult females. The E2 levels decreased after an injection of 1,000 IU h CG on day 10, and the levels on day 21 were almost equal to the predosing values on day 0. On the other hand, the P levels increased transiently on day 16, which suggested ovulation. In the histopathological examination of the ovaries obtained on day 10, large antrum follicles were observed. These results confirmed that the ovary of infant cynomolgus monkeys reacted in the same way as the ovary of adult monkeys to exogenous gonadotropins and suggested that this method could become a useful means of evaluating the ovarian function of infant females.

scholarly journals Assessment of miR-181b-5p, miR-23a-3p, BCL-2, and IL-6 in Peripheral Blood Mononuclear Cells of Autistic Patients; Likelihood of Reliable Biomarkers

2020 ◽  
Author(s):  
Hossein Atwan ◽  
Mohammad-Ali Assarehzadegan ◽  
Mehdi Shekarabi ◽  
Seyed Mohammad Jazayeri ◽  
Shahram Barfi ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Rna Extraction ◽  
Autism Spectrum ◽  
Repetitive Behaviors ◽  
The Other ◽  
Peripheral Blood Mononuclear ◽  
Other Hand ◽  
Blood Mononuclear Cells

Autism is a neurodevelopmental disorder that is recognized by stereotypic and repetitive behaviors after 2 years of old. Dysregulation of the immune system, especially inflammation which is mostly regulated by IL-6, imposes a deficit in CNS development. Along with this crucial biomarker, researchers have proposed BCL-2, micro RNA-23a-3p (miR-23a-3p), miR-181b-5p as other probable biomarkers involved in inflammation and apoptosis. The aim of the study was to evaluate the alteration in the expression of these biomarkers in a group of autism spectrum disorder (ASD) children. Peripheral blood mononuclear cells (PBMCs) were obtained from 37 autistic patients. After RNA extraction with precipitation method, the Syber green qReal-time Polymerase Chain Reaction (PCR) was performed in order to evaluate the possible alteration in the expression of IL-6, BCL-2, miR-181b-5p, and miR-23a-3p. The results were compared with healthy controls. IL-6 was significantly upregulated in ASD patients (p=0.003). On the other hand, miR-23a was upregulated and BCL-2 downregulated in ASD patients but the changes were not significant. In initial evaluations, expression changes of miR-181b-5p were not statistically significant. However, when Patients were divided into two groups of upregulated and downregulated, re-evaluation showed that both up- (p=0.005) and down-regulation (p=0.004) (i.e. changes regardless of the direction) of miR-181b were significant in autistic children. IL-6 and miR-181b-5p can have proper diagnostic values and are reliable biomarkers with high sensitivity and specificity. On the other hand, PBMC can be utilized for such studies and also evaluation of patients' condition instead of brain tissue as it is less accessible.  

A study of cometary eruptions through OH radio-lines

1999 ◽  
Vol 173 ◽  
pp. 249-254
Author(s):  
A.M. Silva ◽  
R.D. Miró
Keyword(s):  
Short Duration ◽  
Growth Curves ◽  
The Other ◽  
Initial Mass ◽  
Radio Lines ◽  
Other Hand ◽  
Sudden Rise

AbstractWe have developed a model for theH2OandOHevolution in a comet outburst, assuming that together with the gas, a distribution of icy grains is ejected. With an initial mass of icy grains of 108kg released, theH2OandOHproductions are increased up to a factor two, and the growth curves change drastically in the first two days. The model is applied to eruptions detected in theOHradio monitorings and fits well with the slow variations in the flux. On the other hand, several events of short duration appear, consisting of a sudden rise ofOHflux, followed by a sudden decay on the second day. These apparent short bursts are frequently found as precursors of a more durable eruption. We suggest that both of them are part of a unique eruption, and that the sudden decay is due to collisions that de-excite theOHmaser, when it reaches the Cometopause region located at 1.35 × 105kmfrom the nucleus.

Closing the GAP—A 5Å Scanning Microscope

1969 ◽  
Vol 27 ◽  
pp. 6-7
Author(s):  
A. V. Crewe
Keyword(s):  
Normal Form ◽  
The Other ◽  
Resolving Power ◽  
Scanning Microscope ◽  
Conventional Microscope ◽  
Other Hand ◽  
Closing The Gap ◽  
Thermal Sources ◽  
Better Than ◽  
Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

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