scholarly journals TOXICITY TEST (LD-50) of HERBAL PREPARATIONS Nepenthes ampularia AS The STANDARD of SECURITY of JAMU HYPERTENSION

2021 ◽  
Vol 748 (1) ◽  
pp. 012029
Author(s):  
Mardhiana ◽  
Abdul Rand ◽  
K Harlinda
Keyword(s):  
Medicinal Plant ◽  
Empirical Evidence ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Death Rate ◽  
Artemia Salina ◽  
Toxic Effects ◽  
Water Safety ◽  
Brine Shrimp Lethality ◽  
Herbal Preparations

Abstract The excavation of the potential utilization of herbs as a plant biopharma or as a material to manufacture. There is empirical evidence that Nepenthes has been used as a lowering of hypertension by taking a decoction of its pitchers. For the use and utilization as a medicinal plant need to be done research that refers to the scientifically certified Jamu. The research aims to detect acute toxic effects so that can be obtained the water safety overview Nepenthes pitchers. The toxicity test was carried out by the Brine Shrimp Lethality Test (BSLT), Artemia salina larva with four extract concentrations (0, 10, 500, 1000 ppm). Based on the results of toxic tests showed that the death rate of A. salina at all levels of concentration is 0 deaths. It is evidence that Nepenthes ampularia is not toxic to A. salina..

scholarly journals Uji Toksisitas Ekstrak Batang Pinang Yaki (Areca vestiaria) pada Artemia salina Leach.

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Windy Astuti Tampungan
Keyword(s):  
Medicinal Plant ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Toxicity Level ◽  
Supernatant Solution

AbstrakEfek toksisitas pinang yaki (Areca vestaria) sebagai tumbuhan obat potensial perlu diuji untuk mengetahui ambang batas penggunaannya. Penelitian ini bertujuan untuk menguji tingkat toksisitas ekstrak batang pinang yaki terhadap larva Artemia salina Leach. dengan metode Brine Shrimp Lethality Test. Ekstrak diencerkan dengan konsentrasi 200, 400, 600 dan 800 ppm masing-masing untuk supernatan rendaman pertama (larutan A),supernatan rendaman ke dua (larutan B) dan supernatan rendaman ke tiga (larutan C) . Hasil penelitian menunjukkan bahwa ekstrak batang pinang yaki mempunyai efek toksisitas dengan nilai LC50 sebesar 398,28 ppm untuk larutan A, 390,84 ppm untuk larutan B dan 438,53 ppm untuk larutan C.Kata kunci: batang Areca vestaria,LC50, uji toksisitasAbstractToxicity effect of pinang yaki (Areca vestiaria) as potential medicinal plant should be evaluated to know its application threshold. This research aimed to evaluate the toxicity level of pinang yaki trunk extract on larve of Artemia salina Leach. using the method of Brine Shrimp Lethality Test. Extract was diluted to concentration of 200, 400, 600 and 800 ppm each for solution A (first soaking supernatant), solution B (second soaking supernatant)and solution C (third soaking supernatant). The result of this research showed that extract of pinang yaki trunk had a toxic characteristic, with LC50 value was 398,28, 390,84, and 438,53 ppm respectively for solution A, B and C.Keywords: LC50 , toxicity test, trunk of Areca vestaria

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Uji Toksisitas dengan Metode BSLT dan Analisis Kandungan Fitokimia Ekstrak Daun Soyogik (Saurauia bracteosa DC) dengan Metode Soxhletasi

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 115 ◽  
Author(s):  
Arter Dein Muaja ◽  
Harry S. J. Koleangan ◽  
Max R. J. Runtuwene
Keyword(s):  
Brine Shrimp ◽  
Leaf Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Toxic Effects ◽  
Toxicity Assay ◽  
Percentage Mortality ◽  
Brine Shrimp Lethality

Penelitian ini bertujuan untuk mengetahui toksisitas dari ekstrak daun soyogik menggunakan metode Brine Shrimp Lethality Test (BSLT) dan menentukan kandungan fitokimia daun soyogik (Saurauia bracteosa DC). Ekstrak dibuat dengan cara soxhletasi menggunakan pelarut metanol. Uji toksisitas dilakukan dengan menggunakan larva udang Artemia salina Leach yang berumur 48 jam. Efek toksik ekstrak diidentifikasi dengan presentase kematian larva udang menggunakan analisis probit (LC50). Kandungan fitokimia meliputi fenolik, flavonoid dan tanin. Penelitian menunjukkan ekstrak daun soyogik bersifat toksik (LC50: 35,4 ppm). Kandungan senyawa fenolik (128 ppm), flavonoid (44,4 ppm), tanin (86,75 ppm).The aims of this research were to determine the toxicity of soyogik (Saurauia bracteosa DC)leaf extract using Brine Shrimp Lethality Test (BSLT) and the content of its compounds. The extraction was carried out ​​by soxhletation using methanol. Toxicity assay used Artemia salina Leach larvae of 48-hours age. Toxic effects of the extract were identified by the percentage mortality of shrimp larvae using probit analysis (LC50). Leaf extract was furthertested to phenolic, flavonoid and tannin contents. The results showed soyogik leaf extract was toxic (LC50: 35,4 ppm). The content of phenolic, flavonoid, and tannin compounds were 128 ppm, 44,4 ppm and 86,75 ppm, respectively.

scholarly journals UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2020 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Arinta Mayang ◽  
Bilal S A Santoso
Keyword(s):  
Acute Toxicity ◽  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Chemical Contents

ABSTRACTAnnona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsakleaves contain alkaloids, tannins, and several other chemical contents including acetogenin.The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves onthe Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimentalstudy used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemiasalina larvae used for each concentration was 10 larvae. The number of dead larvae wascounted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leafinfusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves istoxic.Keywords: Sirsak leaves, toxicity, BSLTABSTRAKSirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanamanobat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasukacetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infusdaun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larvaArtemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva matidihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsakadalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.Kata kunci: daun sirsak, toksisitas, BSLT

scholarly journals Uji toksisitas fraksi aktif ekstrak etanol daun ginje (Thevetia peruviana) dengan metode Brine Shrimp Test dan profil kandungan kimia fraksi teraktif

2013 ◽  
Vol 11 (2) ◽  
pp. 48-57
Author(s):  
VIANA NINGSIH ◽  
ESTU RETNANINGTYAS NUGRAHENI ◽  
OKID PARAMA ASTIRIN
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Toxic Effects ◽  
Active Fraction ◽  
Thevetia Peruviana ◽  
Fraction I

Ningsih V, Nugraheni ER, Astirin OP. 2013. Toxicity test of active fraction from ethanol extract of ginje (Thevetia peruviana) leaves by Brine Shrimp Test method and chemistry compound profile of most active fraction. Biofarmasi 11: 48-57. Cancer is a major threat to human health. The treatment that been made such as dissection, radiation, and chemotherapy, did not give satisfactory results, even gave the considerable adverse side effects. Other alternative that selected is utilizing the natural materials from plant that expected to be companion chemotherapy. One of the plants as source of new drug potentially is ginje (Thevetia peruviana Merr). Previous studies reported that ethanol extract of ginje leaves have toxic effects below 1000 μg/mL. Therefore, it needs to be tested further to determine its potential as anticancer. This study aimed to determine the toxic effects of the active fractions of ethanol extract of ginje leaves by Brine Shrimp Test (BST) method and to determine the profile of active fraction of chemical constituents. Toxicity test was performed with Brine Shrimp Test (BST) method against Artemia salina Leach. Shrimp larva with a series concentrations extract of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL of each concentration were tested in 5 flacons. Each flacon contained 10 shrimp larva. The percentage of shrimp larva mortality was calculated after 24 hours of test and the data were analyzed by calculating the value of LC50. The profile of the chemical contents of the most active fraction were identified by TLC and analyzed qualitatively. The results showed that the most active fraction in killing the larva of shrimp Artemia is the fraction I with the values of LC50-24 hours was 44.67 μg/mL. The contents of chemical compounds contained in fraction I of leaf ethanol extract of ginje were terpenoid and phenolic groups.

scholarly journals Analisis Senyawa Toksik Tepung Pelepah Batang Aren (Arenga pinnata) dengan Spektroskopi UV-Vis dan Inframerah

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 54
Author(s):  
Irma Dukomalamo ◽  
Meiske Sientje Sangi ◽  
Johnly Alfreds Rorong
Keyword(s):  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Column Chromatography ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Functional Group ◽  
Artemia Salina ◽  
Toxic Compounds ◽  
Brine Shrimp Lethality ◽  
Layer Chromatography

Telah dilakukan penelitian mengenai analisis senyawa toksik tepung pelepah batang aren (A. pinnata) dengan spektroskopi UV-Vis dan inframerah dengan tujuan untuk mengisolasi senyawa toksik dari tepung pelepah batang aren dan menganalisis senyawa toksik tersebut dengan spektroskopi UV-Vis dan inframerah. Tepung pelepah batang aren diekstraksi dengan cara maserasi menggunakan pelarut etanol hasil redestilasi cap tikus. Ekstrak diuji dengan Kromatografi Lapis Tipis (KLT) untuk menentukan jenis eluen terbaik untuk dipisahkan dengan kromatografi kolom sehingga diperoleh fraksi-fraksi. Fraksi hasil kromatografi kolom diuji KLT ulang, noda sama dari beberapa fraksi digabungkan. Fraksi yang hanya memiliki 1 noda dilakukan uji toksisitas dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva udang Artemia salina L. dan dianalisis menggunakan spektroskopi UV-Vis dan inframerah. Hasil pengujian KLT diperoleh 6 noda dengan nilai Rf yaitu noda (a) 0,961; (b) 0,857; (c) 0,779; (d) 0,714; (e) 0,623; (f) 0,376. Fraksi A hasil kromatografi kolom dengan noda tunggal yang dilakukan uji KLT Rf = 0,961. Fraksi A yang diuji toksisitas menunjukkan fraksi bersifat toksik dengan nilai LC50 sebesar 269,15 ppm. Analisis spektrofotometer UV-Vis dan Inframerah menunjukkan bahwa fraksi memiliki gugus fungsi –C=O, -C-O ester, -C=C alkena, serta C-C dan C-H alkana. Dari hasil penelitian dapat disimpulkan bahwa senyawa yang diisolasi dari tepung pelepah batang aren bersifat toksik dengan nilai LC50 sebesar 269,15 ppm dan merupakan senyawa nonpolar yang memiliki gugus fungsi –C=O, -C-O ester, -C=C alkena, serta C-C dan C-H alkana.A research has been done about Analysis of Toxic Compounds Sugar Palm Stem Midrib Flour (A. Pinnata) using UV-Vis and Infrared Spectroscopy, aimed to isolated of toxic compounds sugar palm stem midrib flour and analyze using UV-Vis and infrared spectroscopy. Sugar palm stem midrib flour extracted by maceration using ethanol as solvent results from redistillation cap tikus. The extract was tested by Thin Layer Chromatography (TLC) to determine the best eluent for separated with column chromatography to obtained fractions. Fractions column chromatography results were tested using KLT, the same spot of fractions were combined. Fraction having 1 spot was toxicity test performed by the method of Brine Shrimp Lethality Test (BSLT) using Artemia salina L. larvae and analyze using UV-Vis and infrared spectroscopy. Results KLT testing obtained six spots with Rf is  (a) 0,961; (b) 0,857; (c) 0,779; (d) 0,714; (e) 0,623; (f) 0,376. Fraction A column chromatography results having one spot by KLT test with Rf = 0,961. The results of toxicity test showed fraction A was toxic with LC50 269,15 ppm. Analysis spectrophotometer UV-Vis and inframerah showed that fraction had functional group like –C=O, -C-O esther, -C=C alkene, C-C and C-H alkane. This research concludes compound of sugar palm stem midrib flour isolated sugar palm stem midrib flour and nonpolar compound with functional group like –C=O, -C-O esther, -C=C alkene, C-C and C-H alkane.

scholarly journals Toxicity test of active fraction from ethanol extract of ginje (Thevetia peruviana) leaves by Brine Shrimp Test method and chemistry compound profile of most active fraction

2017 ◽  
Vol 11 (1) ◽  
pp. 48-57
Author(s):  
VIANA NINGSIH ◽  
ESTU RETNANINGTYAS NUGRAHENI ◽  
OKID PARAMA ASTIRIN
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Toxic Effects ◽  
Active Fraction ◽  
Thevetia Peruviana ◽  
Fraction I

Ningsih V, Nugraheni ER, Astirin OP. 2013. Toxicity test of active fraction from ethanol extract of ginje (Thevetia peruviana) leaves by Brine Shrimp Test method and chemistry compound profile of most active fraction. Biofarmasi 11: 48-57. Cancer is a major threat to human health. The treatment that been made such as dissection, radiation, and chemotherapy, did not give satisfactory results, even gave the considerable adverse side effects. Other alternative that selected is utilizing the natural materials from plant that expected to be companion chemotherapy. One of the plants as source of new drug potentially is ginje (Thevetia peruviana Merr). Previous studies reported that ethanol extract of ginje leaves have toxic effects below 1000 µg/mL. Therefore, it needs to be tested further to determine its potential as anticancer. This study aimed to determine the toxic effects of the active fractions of ethanol extract of ginje leaves by Brine Shrimp Test (BST) method and to determine the profile of active fraction of chemical constituents. Toxicity test was performed with Brine Shrimp Test (BST) method against Artemia salina Leach. Shrimp larva with a series concentrations extract of 62.5 µg/mL, 125 µg/mL, 250 µg/mL, 500 µg/mL and 1000 µg/mL of each concentration were tested in 5 flacons. Each flacon contained 10 shrimp larva. The percentage of shrimp larva mortality was calculated after 24 hours of test and the data were analyzed by calculating the value of LC50. The profile of the chemical contents of the most active fraction were identified by TLC and analyzed qualitatively. The results showed that the most active fraction in killing the larva of shrimp Artemia is the fraction I with the values of LC50-24 hours was 44.67 µg/mL. The contents of chemical compounds contained in fraction I of leaf ethanol extract of ginje were terpenoid and phenolic groups.

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