scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Skrining Fitokimia Dan Uji Toksisitas Ekstrak Biji Aren (Arenga pinnata MERR.)

Jurnal MIPA ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 12 ◽  
Author(s):  
Dhea Amelia Arief ◽  
Meiske Sangi ◽  
Vanda S. Kamu
Keyword(s):  
Regression Analysis ◽  
Secondary Metabolites ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Triterpenoid Saponin ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Palm Sugar

Penelitian ini bertujuan untuk melakukan skrining fitokimia dari Biji Aren (Arenga pinnata Merr.) dan menentukan toksisitas dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Ekstrak etanol dari biji aren mengandung beberapa senyawa metabolit sekunder seperti flavonoid, triterpenoid, saponin dan tanin sebagai hasil uji fitokimia. Uji toksisitas terhadap indikator larva udang Artemia salina Leach. Menunjukkan nilai LC50 bersifat toksik. Nilai LC50 yang diperoleh sebesar 50, 64 ppm yang ditentukan dengan analisis regresi menggunakan MS Office Excel 2007The research aimed to recognize the phytochemical screening and to determine the toxicity on sugar palm (Arenga pinnata Merr.) seed using Brine Shrimp Lethality Test (BSLT) . Ethanol extract of palm sugar seed contained some compounds secondary metabolites such as flavonoid, triterpenoid, saponin, and tannin as a result of phytochemical test. The toxicity test against animal indicator shrimp Artemia salina Leach showed LC50 values were obtained at 50,64 ppm determined by regression analysis using MS Office Excel 2007.

scholarly journals Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Batang Bawang Laut (Proiphys amboinensis (L.) Herb.)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 144
Author(s):  
Chendy Christy Dapas ◽  
Harry S. J. Koleangan ◽  
Meiske S. Sangi
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Steroid Saponin ◽  
Brine Shrimp Lethality ◽  
Probit Method ◽  
Dried Extract

Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman bawang laut (Proiphys amboinensis (L.) Herb.) Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia untuk senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak batang tanaman bawang laut segar dan kering dan selanjutnya dianalisis menggunakan spektrofotometer UV-VIS. Penentuan toksisitas ekstrak batang tanaman bawang laut menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh flavonoid dan  steroid positif sedangkan alkaloid, saponin triterpenoid dan tanin negatif. Analisis spektofotometer UV-VIS ekstrak etanol menunjukkan puncak serapan pada 304,40 dan  284,50 nm. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan  metode probit menggunakan perangkat lunak SPSS 20. Hasil uji toksisitas ekstrak batang tanaman bawang laut menunjukkan bahwa  ekstrak segar maupun kering bersifat sangat toksik dengan nilai LC50 kurang dari 30 ppm, yaitu: 9,978 ppm untuk batang kering dan 3,980 ppm untuk batang segar.A research has been done on the analysis of secondary metabolites and toxicity examination on the stems of the sea onion (Proiphys amboinensis (L.) Herb.). Analysis of secondary metabolites carried out with the phytochemical screening for alkaloid compounds, flavonoids, triterpenoids, steroids, saponins, and tannins in extracts of fresh and dried stems of the sea onion, then analyzed using UV-VIS spectrophotometer. The toxicity determination of stems extract of the sea onion plant was done by using Brine Shrimp Lethality Test (BSLT) with Artemia salina Leach as bioindicator. Phytochemical screening showed positive test for flavonoids and steroids while alkaloids, saponins, triterpenoids, and tannins were negative. UV-VIS  spectrophotometer analysis on ethanol extract showed peaks at 304,40 and 284,50 nm. Data of the toxicity examination was obtained from the analysis of Lethality Concentration 50 (LC50) which were performed with the probit method using SPSS 20. Results of toxicity examination of sea onion stems extract showed that fresh or dried extract are very toxic with LC50 values ​​of less than 30 ppm, namely: 9,978 ppm for fresh stems and 3,980 ppm for dried stems.

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals 6. Cytotoxic Potential of n-Hexane Extract of Calotropis gigantea L. Leaves

2016 ◽  
Vol 1 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Kartini Hasballah ◽  
Murniana Murniana ◽  
Al Azhar ◽  
Nurul Rahmi
Keyword(s):  
Liquid Chromatography ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Calotropis Gigantea ◽  
Brine Shrimp Lethality ◽  
Vacuum Liquid Chromatography

The present study was done to determine the cytotoxic potential of n-hexane extract of Calotropis gigantea L. leaves and its fractions. Here, dried leaves of C. gigantea L. were macerated using n-hexane to obtain crude extract of 21.16 g (1.03%). The components of n-hexane extract of C. gigantea L. leaves were separated with vacuum-liquid chromatography resulted 4 fractions which are A (0.5 g), B (0.9g), C (5.29 g), and D (6.25 g). Phytochemical screening indicated that the extract contained various secondary metabolic compounds such as steroids, terpenoids, saponins, flavonoids, coumarins, and phenolics. Cytotoxic potenital of the crude extract of C. gigantea L. and its fractions was examined using brine shrimp lethality test (BSLT). The LC50 values of C. gigantea’s n-hexane extract and its fractions that determined by Finney method were 272.27 (n-hexane extract), 31.62 (fraction A), 43.65 (fraction B), 33.89 (fraction C) and 20.98 (fraction D) ?g/mL, respectively. The most active was D fraction contained terpenoids, flavonoids, and phenolics which were assumed contribute to its cytotoxic potential. These results suggested that n-fraction of C. gigantea might possess antitumor or pesticidal activities..

scholarly journals Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract

2011 ◽  
Vol 2 (1) ◽  
pp. 193 ◽  
Author(s):  
Shirly Kumala ◽  
Dwi Windi Sapitri
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Screening ◽  
Toxicological Evaluation ◽  
Brine Shrimp Lethality ◽  
Toxicological Effect ◽  
Toxicological Assessment ◽  
Study Results

Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were no scientific studies including toxicological assessment on the plan extract. The purpose of this study was to investigate the toxicological effect of some fractions of Prasman leaves methanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extract were conducted followed by toxicity test using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroid and volatile oil. LC50 of the n-hexane fraction 238.66 µg/mL, ethyl acetate fraction 24.42 µg/mL,  and n-butanol 64.10 µg/mL.Keywords : BSLT, Toxicity test, Eupatororium triplinerve

scholarly journals Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic screening of the Crude Extracts

2012 ◽  
Vol 60 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Monirul Islam ◽  
MD Al-Amin ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Mohammad Majedul Haque ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Quercetin-3-O-beta-D-glucopyranoside was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethylacetate and butanol soluble fractions of this ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10328Dhaka Univ. J. Sci. 60(1): 11-14, 2012 (January)

scholarly journals Uji Toksisitas Ekstrak Etanolik Batang Tanaman Majapahit (Crescentia cujete) Terhadap Artemia salina Leach

2021 ◽  
Vol 11 (1) ◽  
pp. 7-15
Author(s):  
Rahma Diyan Martha ◽  
Fatimah
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Active Component ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Safe Alternative ◽  
Stem Extract ◽  
Plant Stem ◽  
Crescentia Cujete

Cancer is a disease characterized by uncontrolled cell growth and the ability of these cells to attack biological tissue. Various attempts have been made, one of them with treatment to kill cancer cells, but not a few of these efforts actually cause side effects. This fact demands the need for a safe alternative way to treat cancer using natural ingredients. One of the local natural resources that has the potential to treat cancer is Majapahit (Crescentia cujete). In this study, Majapahit (C. cujete) stem extracts were carried out which have anticancer activity. The first stage was screening by conducting a qualitative analysis of the ethanol extract of the stem of the majapahit plant (C. cujete). Furthermore, a toxicity test was carried out against Artemia salina Leach using the Brine Shrimp Lethality Test (BST) method. Phytochemical testing was carried out to check the content of secondary metabolite compounds contained in the majapahit stem extract, and the results were positive for flavonoids, tannins, saponins and phenolics. This data, supported by spectrophotometric tests, proved that the largest concentration of the ethanolic extract of the majapahit (C. cujete) stem was 24.05% tannin. Then the LC50 test from the toxicity test of the active component of majapahit plant stem extract (C. cujete) against Artemia salina Leach showed a concentration of 0.01 µg / mL and 0.04 µg / mL, namely with a percentage below 50%.

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