Masculinization of beta fish larvae Betta splendens through the different treatment immersion of honey solution and larval age

Larval of fighting fish (Betta splendens Regan) requires precise live foods for its growth and survival. In this experiment, fish larvae were fed on either Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, or Paramecium + Moina + Tubifex. The fish were fed Paramecium from day-2 till day-7 after hatching. There after, the live food was changed according to the treatments till day-28. Results showed that fish fed on Paramecium + Artemia significantly had the highest total length (12.63 mm) than other treatments (11.86 mm). On the other hand, survival rate of fish had no significant affected by the treatments. Keywords: fighting fish, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larvae ABSTRAK Larva ikan betta (Betta splendens Regan) membutuhkan jenis pakan alami yang tepat bagi kelangsungan hidup dan pertumbuhannya. Pada penelitian ini, larva ikan diberi pakan berupa Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, atau Paramecium + Moina + Tubifex. Ikan diberi pakan pakan berupa Paramecium dari hari ke-2 hingga hari ke-7. Setelah itu, pemberian pakan alami diubah berdasarkan masing-masing perlakuan hingga hari ke-28. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan Paramecium + Artemia memiliki tubuh secara signifikan lebih panjang (12,63 mm) dibandingkan perlakuan lainnya (11,86 mm). Sementara itu, kelangsungan hidup tidak dipengatuhi oleh perlakuan. Kata kunci: ikan betta, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larva

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Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens

DEPIK ◽

10.13170/depik.8.3.13916 ◽

2019 ◽

Vol 8 (3) ◽

pp. 235-242

Author(s):

Mustaqim Mustaqim ◽

Kartini Eriani ◽

Erlangga Erlangga ◽

Rachmawati Rusyidi

Keyword(s):

Water Quality ◽

Fish Larvae ◽

Fertilization Rate ◽

Quality Parameters ◽

Betta Splendens ◽

Effect Of Temperature ◽

Hatching Rate ◽

Egg Hatchability ◽

Hatching Time ◽

Randomized Design

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak. Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

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SEX REVERSAL IN SIAMESE FIGHTING FISH LARVAE BY THERMAL MANAGEMENT

Boletim do Instituto de Pesca ◽

10.20950/1678-2305/bip.2021.47.e676 ◽

2021 ◽

Vol 47 ◽

pp. e676

Author(s):

Igor de Fiuza FRANÇA ◽

Guilherme Melgaço HELUY ◽

Érica Beatriz SCHULTZ ◽

Wilson de Oliveira VIANNA ◽

Marcelo Maia PEREIRA ◽

...

Keyword(s):

Sex Ratio ◽

Thermal Management ◽

Fish Larvae ◽

Betta Splendens ◽

Thermal Treatments ◽

Randomized Design ◽

Males And Females ◽

Completely Randomized Design ◽

Newly Hatched Larvae ◽

Number Of Males

The present study evaluated the influence of thermal management during the larviculture of Betta splendens on survival and sex ratio, aiming to increase the proportion of males. Newly hatched larvae were subjected to different thermal regimes, namely, T25, T28, T30 and T33 (25, 28, 30 and 33ºC, respectively). The experiment was laid out in a completely randomized design, with 4 treatments and 10 repetitions. Thermal treatment was maintained until 15 days post-hatch (DPH). Mortality was determined at the end of the thermal regime and again at 45 DPH. At the end of the experiment, the number of males and females obtained in the different thermal treatments was counted to analyze the obtained sex ratio. There was a significant effect on mortality as a function of temperature only at 15 DPH (p <0.001), with the lowest values recorded in treatments T25, T28 and T30. In terms of sex ratio, up to 65% of males were obtained in treatment T33 (p = 0.037). In conclusion, thermal management during the larval period can be a strategy to increase the proportion of males, but the increase in mortality due to the rise in temperature should be considered.

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MASKULINISASI IKAN CUPANG (Betta splendens) MENGGUNAKAN MADU ALAMI MELALUI METODE PERENDAMAN

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v6i2.7158 ◽

2018 ◽

Vol 6 (2) ◽

pp. 141-152

Author(s):

Annisa Siregar ◽

Mochamad Syaifudin ◽

Marini Wijayanti

Keyword(s):

Water Quality ◽

Fish Larvae ◽

Larval Survival ◽

Betta Splendens ◽

Survival Percentage ◽

Randomized Design ◽

Natural Honey

ABSTRACT This research aims to determine the effect of natural honey to male percentage of betta fish by masculinization. This researchwas conducted at FisheriesBasic Laboratory, Faculty of Agriculture, Sriwijaya University on January– February 2018. This research usedcompletely randomized design (CRD) with 5 treatments and 3 replications. The treatment in this research was4 day of betta fish larvae that submersed in 5 ml/L natural honey with different immersiontime.The treatmentconsisted of P0 (without natural honey), P1 (12 hours of immersion), P2 (16 hours of immersion), P3 (20 hours of immersion),and P4 (24 hours of immersion). The observed parameters were post-immersion survival percentage, percentage of male betta fish, post- rearing survival percentage and water quality. The result showed the percentage of male betta fish was 47,50 % (P0), 66,35 % (P1), 73,51% (P2), 75,37 (P3), and 85,14 % (P4). Water quality during maintenace were temperature 27,4-28,8 oC, pH 5,3-5,8, DO 3,43-3,59 mg/L and amonia 0’001-0,002 mg/L. Treatment of P4 (24 hours of immersion) indicatedthe best treatment with larval survival percentage andmale percentage were 90.00% and 85.14% respectirely. Keywords : Betta fish (Betta splendens), masculinization,natural honey

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Masculinizing Effects of Chrysin-Loaded Poloxamer Micelles on Siamese Fighting Fish

Veterinary Sciences ◽

10.3390/vetsci8120305 ◽

2021 ◽

Vol 8 (12) ◽

pp. 305

Author(s):

Nattakanwadee Khumpirapang ◽

Tanongsak Sassa-deepaeng ◽

Krit Suknuntha ◽

Songyot Anuchapreeda ◽

Siriporn Okonogi

Keyword(s):

Survival Rate ◽

Polymeric Micelles ◽

Fish Larvae ◽

Siamese Fighting Fish ◽

Betta Splendens ◽

In Vivo Studies ◽

High Survival ◽

Low Toxicity ◽

High Fish

Siamese fighting fish (Betta splendens) are freshwater fish that are commonly found in Thailand and other Southeast Asian countries. In the present study, chrysin-loaded polymeric micelles (CPs) were developed and investigated for the masculinizing effects, survival rate, growth indices, and toxicity on Siamese fighting fish. CPs were prepared using a poloxamer. The micelle system of CPs that were formed at a chrysin-to-polymer ratio of 1:2 was found to be the most suitable monodispersed system and exhibited a nanosized diameter (74.2 ± 1.6 nm) with a narrow size distribution (0.288 ± 0.012). In vivo studies were performed using Siamese fighting fish larvae as animal models. In the in vivo toxicity study, the fish larvae were immersed in aqueous systems containing CPs that had five different chrysin concentrations of 1, 10, 100, 1000, and 10,000 ng/mL for 24, 48, and 72 h. Blank polymeric micelles and water were used as controls. The in vivo masculinization effect of CPs with different chrysin concentrations on the fish larvae was evaluated after 5 weeks of exposure. The results demonstrated that CPs with a chrysin concentration of 1000 ng/mL showed a masculinization effect of 94.59 ± 2.76% with a high fish larvae survival rate of 72.45 ± 5.09% and low toxicity. It was concluded that the developed CPs had a significant effect on the sex reversal of Siamese fighting fish larvae with a high survival rate.

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PERBEDAAN WAKTU PEMELIHARAAN TELUR DAN LARVA OLEH INDUK JANTAN TERHADAP DAYA TETAS DAN KELANGSUNGAN HIDUP LARVA IKAN CUPANG (Betta splendens)

Jurnal Akuakultur Sungai dan Danau ◽

10.33087/akuakultur.v3i2.40 ◽

2018 ◽

Vol 3 (2) ◽

pp. 76

Author(s):

M Fauzan ◽

Muhammad Sugihartono ◽

M Yusuf Arifin

Keyword(s):

Survival Rate ◽

Water Level ◽

Sea Water ◽

Fish Larvae ◽

Ornamental Fish ◽

Betta Splendens ◽

Male Parent ◽

Long Time ◽

Absolute Length ◽

Body Shapes

Ornamental fish is a type of fish that lives in both fresh and sea water which has attractive and beautiful body shapes or colors. One type of ornamental fish with its own uniqueness compared to other ornamental fish is Betta fish (B. splendens.). As an alternative, the test was carried out for a long time the male parent who cared for larvae at different times on the effect of survival of betta fish larvae on the type of plaque (B. splendens). The parent used in this study is a parent of 4 months old which is prepared as many as 1 pair / container of maintenance and in total there are 12 pairs. The research container used in this study was an aquarium with a water level of 10 cm, 12 pieces. Each container is labeled according to randomization and is equipped with aeration with a small pressure. The water used in this study is water originating from excavated wells. Before use water is first deposited to remove sedimentation. For the best treatment, the highest survival rate of betta (B. splendens) larvae was found in treatment A, which was 11.56%, followed by treatment B (8.44%), then treatment C (7.51%) and treatment D (4.81%). The highest absolute length growth is found in treatment D, which is equal to 14.33 mm, followed by treatment C (14.00 mm), then treatment B (12.83 mm) and treatment A (11.17 mm).Keywords: Plaque Betta Fish, Maintenance Length by Male Parent, Survival, Growth, Water Quality.AbstrakIkan hias merupakan jenis ikan yang hidup di air tawar maupun laut yang mempunyai bentuk atau warna tubuh menarik dan indah. Salah satu jenis ikan hias dengan keunikan tersendiri dibandingkan ikan hias lainnya adalah ikan cupang (B. splendens.). Sebagai alternatif uji yang dilakukan adalah lama induk jantan yang mengasuh larva dengan waktu yang berbeda terhadap pengaruh kelangsungan hidup larva ikan cupang jenis plakat (B. splendens).Induk yang digunakan dalam penelitian ini adalah induk yang berumur 4 bulan yang di persiapkan sebanyak 1 pasang / wadah pemeliharaan dan secara total berjumlah 12 pasang.Wadah penelitian yang digunakan dalampenelitian ini adalah akuarium dengan ketinggian air 10 cm, sebanyak 12 buah. Masing-masing wadah diberi label sesuai dengan pengacakan dan dilengkapi aerasi dengan tekanan yang kecil. Air yang digunakan dalam penelitian ini adalah air yang berasal dari sumur galian. Sebelum digunakan air terlebih dahulu diendapkan untuk menghilangkan sedimentasi. Untuk perlakuan yang terbaik menunjukkan bahwa tingkat kelangsungan hidup larva ikan cupang (B. splendens) tertinggi terdapat pada perlakuan A yaitu sebesar 11,56% selanjutnya diikuti perlakuan B (8,44%), kemudian perlakuan C (7,51%) dan perlakuan D (4,81%). Pertumbuhan panjang mutlak tertinggi terdapat pada perlakuan D yaitu sebesar 14,33 mm selanjutnya diikuti oleh perlakuan C (14,00 mm), kemudian perlakuan B (12,83 mm) dan perlakuan A (11,17 mm).Kata Kunci :Ikan cupang Plakat, Lama Waktu Pemeliharaan Oleh Induk Jantan, Kelangsungan Hidup, Pertumbuhan, Kualitas air.

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