scholarly journals Comparative study of herbal and non-herbal egg protein profile using High Performance Liquid Chromatography (HPLC)

2021 ◽  
Vol 888 (1) ◽  
pp. 012019
Author(s):  
N Ardiarini ◽  
J A Lase
Keyword(s):  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
High Performance ◽  
Protein Profile ◽  
Egg Yolk ◽  
Peak Height ◽  
Yolk Protein ◽  
Antioxidant Compounds

Abstract The purpose of this research is to compare the protein profile of herbals egg and non herbal egg using High Performance Liquid Chromatography (HPLC). This research used HPLC Shimadzu 6.1 with column C18, flow rate mobile phase 1 ml/min, wavelenght detector UV Vis 220 nm and temperature 50o C. Mobile fase that used in this research was 10 and 60 % acetonitril (CH3CN) in water containing 0,05% triflouroacetic acid. Supplementation of herb containing bioactive antioxidant compounds affect the formation of egg yolk protein containing immunoglobulin. In this study, herbal eggs and non-herbal eggs were seen from their protein profile using the high performance liquid chromatography (HPLC) method and then analyzed using descriptive qualitative analysis. The result show that herbal egg yolk sample has a dominant protein with a molecular weight of 50.41 kDa. Herbal egg yolk protein appears at a retention time (RT) of 56.87 minutes, an area of 3303488 units of area, and the peak height of the graph / peak at 50,974 µAU. Meanwhile, non-herbal egg yolk sample has a dominant protein with a molecular weight of 49.94 kDa. This protein appears at a retention time of 1.307 minutes, an area of 149445550 units of area, and the peak height of the graph / peak at 402.6026 µAU. The results showed that the the peak of HPLC indicated an antioxidants were bound to the bioactive protein fractions of egg yolk. It could be concluded that bioactive herbal bound to egg yolk igY, but the bioactive compounds have not been identified yet.

scholarly journals Determination of 11 Low-Molecular-Weight Carbonyl Compounds in Marine Algae by High-Performance Liquid Chromatography

2006 ◽  
Vol 44 (5) ◽  
pp. 233-238 ◽  
Author(s):  
V. M. da Silva ◽  
M. C. da Cunha Veloso ◽  
E. T. Sousa ◽  
G. Vieira Santos ◽  
M. C. Accioly ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbonyl Compounds ◽  
High Performance ◽  
Marine Algae ◽  
Low Molecular Weight

scholarly journals The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Lin-Jiao Wang ◽  
Mao-Yin Sheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Interspecific Hybridization ◽  
Retention Time ◽  
High Performance ◽  
Symmetry Type ◽  
Hplc Fingerprint ◽  
C Banding ◽  
Eleven Species

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

Sign in / Sign up

Export Citation Format

Share Document