Morphological characterization of natural orchids Dendrobium spp.

Abstract Dendrobium is characterized by long pseudobulbs or canes with soft leaves over the entire length, or in some species short or swollen pseudobulbs with two leathery leaves. The inflorescence is composed from dozens of flowers of different sizes and colors. This study aimed to identify the quantitative morphological character of five species of Dendrobium spp. namely D. mirbelianum, D. lamellatum from Java, D. anosmum from South Kalimantan, D. bracteosum from Papua, and D. purpureum from North Sumatera. The resulted dendrogram based on the similarity matrix were divided into two clusters, among the five species the value of similarity coefficient is 1.50. The first cluster is only composed from D. mirbelianum, the second cluster is D. lamellatum, D. purpureum, D. bracteosum, and D. anosmum which have more distant relationship with the other three orchids. Moreover, D. lamellatum and D. purpureum have the closest similarity coefficient with 0.81 value, which have bigger chance to use as the parents for hybridization. There are many Dendrobiums spp. distribution which based on the relationship area. In addition to quantitative properties, it also needs to be expanded to qualitative, anatomy, cytology, and also molecular characteristic to have more comprehensive data.

Download Full-text

Morpho-molecular characterization of Bipolaris and Exserohilum spp. infecting various agricultural crops

International Journal of Agricultural and Applied Sciences ◽

10.52804/ijaas2021.2111 ◽

2021 ◽

Vol 2 (1) ◽

pp. 110-117

Author(s):

Seema Singh ◽

◽

Sneha Sharma ◽

Aaqib Ali ◽

A Kandan ◽

...

Keyword(s):

Molecular Characterization ◽

Plant Pathogens ◽

Morphological Characterization ◽

Similarity Coefficient ◽

Agricultural Crops ◽

Similarity Matrix ◽

Major Objective ◽

Isolation And Purification ◽

Issr Primers

The study was undertaken with major objective to differentiate fungal isolates belonging to various species of Bipolaris and Exserohilum infecting various agricultural crops. After routine isolation and purification of more than 35 attempts, 13 isolates were morphologically characterized into 8 species namely, Bipolaris cynodontis (IQBC-1 and DQBC-2), B. holmii (IQBH-1), B. micropus (IQBM-1), B. oryzae (DQBO-1, DQBO-2), B. sorghicola (DQBS-1, DQBS-2), B. tetramera (DQBT-1, DQBT-2), Exserohilum longirostrata (IQEL-1) and E. rostratum (DQER-1 and DQER-2). Molecular characterization of all the isolates using RAPD, ISSR and URP markers also revealed genetic variation. Out of 24 RAPD primers, only 9 (OPA-2, OPA-4, OPA-9, OPB-4, OPB-8, OPC-1, OPC-2, OPC-5 and OPE-5) could amplify the DNA sequence showing good polymorphism. Out of 20 ISSR primers, only 6 (ISSR 1, ISSR 13, ISSR 15, ISSR 17, ISSR 19 and ISSR 20) could show good polymorphism. Out of 13 URPs, only 5 (URP 4R, URP 6R, URP 9F, URP 13R and URP 38F) could amplify with a greater number of bands. In all the three makers, similarity matrix value using Jaccard’s coefficient ranged from 0.149 to 0.548 revealing genetic co-relation among isolates. Combined clustering of RAPD, ISSR and URP data showed relatively low genetic similarity coefficient ranging from 14.92 to 54.76 among isolates which revealed stronger co-relation among isolates. Cluster analysis based on UPGMA provided a clear resolution of relationship among all the 13 isolates belong to 6 Bipolaris and 2 Exserohilum species and confirmed the morphological characterization of the same. Further, molecular markers especially, RAPD, ISSR and URP have revolutionized the genetic analysis of plant pathogens including fungi and their application can greatly help in characterizing related genera and delineation of inter and intra-species complex of fungi.

Download Full-text

Morphological characterization of gram-positive and gram-negative bacteria from treated latex processing wastewater

Environmental and Toxicology Management ◽

10.33086/etm.v1i2.2263 ◽

2021 ◽

Vol 1 (2) ◽

pp. 32-36

Author(s):

Farrah Aini Dahalan ◽

Nor Azizah Parmin

Keyword(s):

Oxygen Demand ◽

Morphological Characterization ◽

The Other ◽

Morphological Properties ◽

Morphological Observation ◽

Gram Positive ◽

Bacterial Colony ◽

Gram Negative ◽

Gram Staining

A preliminary morphological screening and isolation of bacterial colony from latex industrial wastewater was carried out. Bacteria colonies from latex processing wastewater were isolated from a local latex processing industry. It was found that 17 bacterial isolates had been purified grown on nutrient agar under 35˚C. The colonies were then purified and morphologically indicated via Gram staining and motility test. After morphological observation, it was identified that out of 17 isolates, 9 isolates were Gram positive and 8 isolates were Gram negative. There are 11 out of 17 colonies were rod-shaped bacterial colonies, while the other 6 colonies were cocci-shaped bacteria. There were 11 colonies of gliding bacteria, three colonies were non-motile bacteria and the other three colonies were flagellated bacteria. This study is only limited to morphological observation as the main aim of this study was to investigate the potential occurrence of viable growth in treated latex processing wastewater. The bacterial colonies were classified base on their morphological properties shown. This study has classified several genera such as Staphylococcus, Escherichia, Thiobacillus, Arthrobacter and other Genus. The growth curve of 17 isolates studied and the chemical oxygen demand were determined.

Download Full-text

Effects of strain on La0.67-x Prx Ca0.33 MnO3, LaMn1-x Cox O3 and LaMn1-x Nix O3 magnetite samples

DYNA ◽

10.15446/dyna.v86n211.78081 ◽

2019 ◽

Vol 86 (211) ◽

pp. 278-287

Author(s):

Javier Alberto Olarte Torres ◽

María Cristina Cifuentes Arcila ◽

Harvey Andrés Suárez Moreno

Keyword(s):

Compressive Strength ◽

Magnetic Susceptibility ◽

Sol Gel ◽

Morphological Characterization ◽

Electric Resistivity ◽

The Other ◽

X Ray Diffraction ◽

Metal Insulator ◽

X Ray

This paper presents the results obtained from the synthesis and morphological characterization of different magnetite samples: La0.67-x Prx Ca0.33 MnO3.LaMn1-x Cox O3 and LaMn1-x Nix O3 at 0.13 ≤ 𝑥𝑥 ≤ 0.67 produced by a solid-state reaction mechanism and 𝐿𝐿𝐿𝐿𝑀𝑀𝑀𝑀1−𝑥𝑥(𝐶𝐶𝐶𝐶/𝑁𝑁𝑁𝑁)𝑥𝑥𝑂𝑂3 at 0.0 ≤ 𝑥𝑥 ≤ 0.5 produced by the sol-gel method. These samples were characterized using X-ray diffraction spectroscopy and by measuring electric resistivity and magnetic susceptibility which were carried out as a function of temperature. Notably, the effects of strain and compressive strength on the lattices of magnetite samples were highly dependent on the concentration of 𝑃𝑃𝑟𝑟, 𝐶𝐶𝐶𝐶, and 𝑁𝑁𝑁𝑁. Moreover, the transition temperatures of metal-insulator and ferromagnetic-paramagnetic phases also largely depend on these strength effects, e.g., at higher concentrations of 𝑃𝑃𝑟𝑟, effects of increased strain strength were observed, relocating the shifts of ferromagnetic-paramagnetic transitions to lower temperatures. On the other hand, effects of increased compressive strength were observed at higher concentrations of 𝑁𝑁𝑁𝑁 and 𝐶𝐶𝐶𝐶, relocating the shifts of ferromagnetic-paramagnetic and metal-insulator transitions to higher temperatures.

Download Full-text

PHENOTYPIC CHARACTERIZATION OF BENECKEA PARAHAEMOLYTICA: A PRELIMINARY REPORT1

Journal of Milk and Food Technology ◽

10.4315/0022-2747-36.4.214 ◽

1973 ◽

Vol 36 (4) ◽

pp. 214-219 ◽

Cited By ~ 6

Author(s):

Paul Baumann ◽

Linda Baumann

Keyword(s):

Liquid Medium ◽

Type Strain ◽

Solid Medium ◽

Morphological Characterization ◽

The Other ◽

Polar Flagellum ◽

Phenotypic Characterization ◽

Phenotypic Traits ◽

General Properties

Eighty-six strains which were isolated from cases of gastroenteritis and had the general properties of the genus Beneckea were submitted to an extensive nutritional, physiological, and morphological characterization. The results indicated that this collection of strains, which included the type strain of Beneckea parahaemolytica, was phenotypically homogeneous and distinguishable from the other known species of Beneckea by multiple, unrelated, phenotypic traits. When grown in liquid medium, strains of B. parahaemolytica had single, sheathed, polar flagella; when grown on solid medium, these strains had unsheathed, peritrichous flagella in addition to the sheathed, polar flagellum. Additional traits of use for differentiation of this species from the remaining species of the genus Beneckea were the ability of B. parahaemolytica to grow at 40 C, utilize d-galactose, l-leucine, l-histidine, and putrescine and the inability to utilize sucrose, dl-β-hydroxy-butyrate or give a positive Voges-Proskauer reaction. The validity of some of the traits previously used to identify B. parahaemolytica as well as the possible difficulties encountered in the identification of this organism from marine sources are considered.

Download Full-text

A relação entre História e Sociologia no horizonte da conceitualização e da explicação dos objetos históricos: reflexões sobre o pensamento de Max Weber * The relationship between History and Sociology over the horizon of conceptualization...

História e Cultura ◽

10.18223/hiscult.v3i3.1408 ◽

2014 ◽

Vol 3 (3) ◽

pp. 28

Author(s):

ULISSES DO VALLE

Keyword(s):

Max Weber ◽

Historical Narrative ◽

The Other ◽

Historical Knowledge ◽

Empirical Reality ◽

Ideal Types ◽

Historical Objects ◽

The One ◽

The Relationship

Resumo: Este artigo procura refletir sobre as relações entre a disciplina da história e a sociologia a partir do pensamento de Max Weber. Procuramos mostrar como a sociologia exerce uma participação fundamental na constituição do conhecimento histórico com relação a dois procedimentos específicos: a caracterização adequada das entidades históricas individuais, por um lado, e a lógica explicativa que preside a narrativa histórica, por outro. Veremos como Weber, então, introduz a sociologia como uma forma de resolver o intricado problema da interpenetração entre o geral e o particular na representação e na explicação dos objetos históricos, de modo a esclarecer os vínculos formais e metodológicos entre as duas disciplinas assim entendidas.Palavras-chave: História; Sociologia; realidade empírica; tipos ideais. Abstract: This paper discuss the relationship between the discipline of history and sociology from the thought of Max Weber. We intend to show how sociology plays a key role in the constitution of historical knowledge regarding two specific procedures: the appropriate characterization of individual historical entities, on the one hand, and the explanatory logic of the historical narrative, on the other. We will see how Weber then introduces sociology as a way to solve the intricate problem of interpenetration between the general and the particular in the representation and explanation of historical objects, in order to clarify the formal and methodological links between the two disciplines well understood.Keywords: History; Sociology; empirical reality; ideal types.

Download Full-text

Introduction pages

Notulae Scientia Biologicae ◽

10.15835/nsb13411137 ◽

2021 ◽

Vol 13 (4) ◽

pp. 11137

Author(s):

Radu E. SESTRAS

Keyword(s):

Biological Properties ◽

Morphological Characterization ◽

Peninsular Malaysia ◽

Species Variation ◽

Root Extract ◽

Biochemical Alterations ◽

Kaempferia Galanga ◽

The Relationship ◽

Trypanosoma Species

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 4, Volume 13, 2021: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting researches or reviews, we invite readers to find news about: Haemato-biochemical alterations and acute toxicity study of hydro-methanol root extract of Anacardium occidentale in cockerels; Diversity of butterflies (Lepidoptera: Rhopalocera) in Bhubaneswar, Odisha, India; Molecular detection of Trypanosoma species and haematological alterations in four trypanosome-infected Nigerian horses; Cryopreservation of aromatic ginger Kaempferia galanga L. by encapsulation-dehydration; Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.; Comparative leaf anatomy of ten Nepenthes L. species (Nepenthaceae) from Peninsular Malaysia; Seed germination response of Indian wild pear (Pyrus pashia) to gibberellic acid treatment and cold storage; Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt.

Download Full-text

Biological and Molecular Characterization of Four Botryosphaeria Species Isolated from Pear Plants Showing Stem Wart and Stem Canker in China

Plant Disease ◽

10.1094/pdis-10-13-1060-re ◽

2014 ◽

Vol 98 (6) ◽

pp. 716-726 ◽

Cited By ~ 24

Author(s):

Lifeng Zhai ◽

Meixin Zhang ◽

Gang Lv ◽

Xiaoren Chen ◽

Nana Jia ◽

...

Keyword(s):

Molecular Characterization ◽

Phylogenetic Analyses ◽

Stem Canker ◽

Morphological Characterization ◽

The Other ◽

Internal Transcribed Spacer Region ◽

Pathogenicity Tests ◽

The Common ◽

Stem Cankers

Pear stem wart and pear stem canker, which have been considered as two different fungal diseases caused by pathogens belonging to Botryosphaeria spp., commonly occur and cause serious damage in the main pear-producing areas in China. To identify the species of this genus infecting pear in China, 131 Botryosphaeria isolates were recovered from pear samples exhibiting symptoms collected from 20 different provinces and areas. Morphological characterization and phylogenetic analyses of the ribosomal DNA internal transcribed spacer region and the β-tubulin and EF1-α genes revealed that Botryosphaeria dothidea, B. rhodina, B. obtusa, and B. parva were associated with different pear stem wart and stem canker symptoms. Remarkably, all isolates of B. dothidea were obtained from the samples showing either stem wart or stem canker lesions; however, the isolates of the other three species were obtained only from the samples showing stem canker. Pathogenicity tests on the pear shoots showed that B. dothidea isolates could induce stem wart or stem canker lesions but all the isolates of the other three species could only induce stem cankers. However, the isolates of B. parva, B. rhodina, and B. obtusa exhibited higher virulence than that of the B. dothidea isolates on the pear fruit. Our results suggest that B. dothidea is the common causal agent for these two diseases (a pear stem wart and a pear-related stem canker), whereas B. parva, B. rhodina, and B. obtusa only cause pear stem canker diseases. To our knowledge, this study represents the first report for biological and molecular characterization of four Botryosphaeria spp. isolated from pear plants showing stem wart and stem canker in China.

Download Full-text

The Paul of Acts

Novum Testamentum ◽

10.1163/15685365-12341644 ◽

2019 ◽

Vol 62 (1) ◽

pp. 60-78

Author(s):

Joshua W. Jipp

Keyword(s):

Acts Of The Apostles ◽

Present Author ◽

The Other ◽

Right Hand ◽

Positive Treatment ◽

Identity Markers ◽

The Relationship ◽

Textual Support

AbstractThe question of the relationship between “Judaism” and “Christianity” in the Acts of the Apostles has been marked by two contradictory interpretive traditions. One tradition emphasizes conflict and rupture, whereas the other sees continuity and a positive treatment of Judaism. These interpretive traditions both find significant textual support from Acts. There is an internal tension within Luke’s characterization of Paul that does not fit neatly into easy dichotomies and is representative of Luke’s broader two-volume work. The present author argues that the significance of God’s history within Israel centers upon Paul’s central conviction that Israel’s Davidic Messiah, resurrected and enthroned at God’s right hand, is the singular dispenser of salvation for Israel and the pagan nations. This messianic conviction results in a re-evaluation (not rejection) of Israel’s primary identity markers that will only be embraced if one grants Paul’s claim that the hope of Israel is identified with Jesus the resurrected Messiah.

Download Full-text

Similarity level of Nepenthes spp. based on the qualitative characters

Ilmu Pertanian (Agricultural Science) ◽

10.22146/ipas.55728 ◽

2020 ◽

Vol 5 (3) ◽

pp. 140

Author(s):

Putri Lukmanasari ◽

Aziz Purwantoro ◽

Rudi Hari Murti ◽

Zulkifli Zulkifli

Keyword(s):

Genetic Relationship ◽

Ornamental Plants ◽

Morphological Characterization ◽

Similarity Coefficient ◽

High Similarity ◽

Pitcher Plant ◽

Group A ◽

Qualitative Characters ◽

Group B ◽

The Relationship

Nepenthes, also known as pitcher plant, are unique and interesting flora developed as ornamental plants. This species has an attraction not only on the flowers but also on the diverse shapes and colors of the pitchers. Each Nepenthes species and hybrid has a variety of characters. The genetic relationship and diversity in Nepenthes species and hybrids can be determined based on the morphological characterization. The purpose of this study was to determine the similarity level among the Nepenthes accessions based on 31 qualitative characters. In this study, 30 Nepenthes species and 11 hybrids were used. The range of the similarity coefficient of Nepenthes species was 0.28-0.65, and divided into two major groups namely group A and B. Group A was the Nepenthes population which had petioles consisted of 17 accessions, while group B had folius consisting of 24 accessions. It indicated that when the similarity value is higher, the similarity level between each plant becomes higher. Principle Coordinates Analysis (PCoA) provided an overview of the relationship among the genotypes in coordinates. Species with a high similarity coefficient were in a close position, whereas species with a low similarity coefficient were in a remote position. Based on the PCoA, the species were grouped based on the similar characters, which was supported by the dendrograms.

Download Full-text

Characterization of Carambola (Averrhoa carambola L.) Plant Collection of Cibinong Plant Germplasm Garden Based on Phenotypic and Genetic Characters

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v8i1.5199 ◽

2016 ◽

Vol 8 (1) ◽

pp. 119

Author(s):

Dody Priadi ◽

Ambar Yuswi Perdani ◽

Yuli Sulistyowati ◽

Fiqolbi Nuro Pohan ◽

Enung Sri Mulyaningsih

Keyword(s):

Phylogenetic Analysis ◽

Rapd Markers ◽

The Other ◽

Plant Production ◽

Averrhoa Carambola ◽

Phenotypic Characters ◽

Plant Collection ◽

The Relationship ◽

Genetic Characters

Indonesia as a rich biodiversity country has many superior fruit plant germplasms such as sweet star fruit or carambola (Averrhoa carambola L.). Some varieties of carambola which collected at the Germplasm Garden of Research Center for Biotechnology-LIPI have been used for parent trees of fruit plant production. Therefore, they have to be characterized both phenotypically and genetically. The objective of the study was to analyze the relationship between eight varieties of carambola i.e. Malaysia, Penang, Rawasari, Bangkok, Sembiring, Dewabaru, Demak and Dewimurni at the germplasm garden based on phenotypic and genetic characters. Phenotypic characters were observed directly in the field, whereas genetic characters were observed with RAPD markers using 10 primers. Phylogenetic analysis was done using NT-SYS software showed that there were three clusters of carambola varieties. Meanwhile, Malaysia and Penang varieties have closed relationships (96%) compared with the other varieties. The result of the study would be dedicated to updating and completing the existing fruit plant collection database of Plants Germplasm Garden.

Download Full-text