scholarly journals Biological Effect of Different Concentrations of Bacillus Thurngensis Isolated From The Soils of Sawa Lake, Al Muthanna Governorate on The of Hypera postica at Different Time Periods

2021 ◽  
Vol 923 (1) ◽  
pp. 012007
Author(s):  
Meeri Kadhim Al-Fatlawi ◽  
Iktifaa Naeem Jasim ◽  
Nidaa Saud AlShammary ◽  
Khudheyer A. A. Alnomani
Keyword(s):  
Biological Effect ◽  
Larval Instar ◽  
Bacterial Isolates ◽  
Hypera Postica ◽  
Killing Rate ◽  
The Third ◽  
The North ◽  
Fourth Larval Instar ◽  
Time Periods

Abstract This study conduct in Al-Muthanna governorate to assess five concentrations of Bacillus thurngensisagonist Hyperapostica. The results showed the presence of Bacillus thurngensisin all the studied sites of Lake Sawa in Muthanna Governorate, and the rates of its presence were close to the same sites, and the rate of its presence in those sites was 35%, and its highest rate was recorded in the north and east of the lake, as it reached 40% and the lowest amounted to 30% in the two sites south and west site. The results of the study showed that five concentrations were taken from bacterial isolates of B. thuringiensisdiffered in the rates of killing larvae, pupae and adults of the insect Hyperapostica in vitro, Where the concentrate 1.7 × 103 spore/ml was characterized by a higher killing ratecompared to the four studied concentrations, the killing rates were (75.750, 71.080, 69.79, 64,361, 49.117 and 42,060)% for the first larval instar, the second larval instar, the third larval instar, the fourth larval instar, and the pupae, respectively, and the lowest killing rate recorded 6.873% at concentration 0.64 × 107after 24 hours of treatment.

Development and differentiation in vitro of Drosophila imaginal disc cells from dissociated early embryos

Development ◽  
1975 ◽  
Vol 33 (2) ◽  
pp. 487-498
Author(s):  
Andreas Dübendorfer ◽  
Glen Shields ◽  
James H. Sang
Keyword(s):  
Imaginal Disc ◽  
Larval Instar ◽  
Cell Types ◽  
The Third ◽  
Early Embryos ◽  
Disc Cells ◽  
Development And Differentiation ◽  
Pattern Specification

Embryos of Drosophila melanogaster, 6–8 h after oviposition, were dissociated and the cells cultured in vitro. Besides larval cell types, imaginal disc cells, assembled and growing in bloated monolayered vesicles, were obtained. The cells of these vesicles become competent to differentiate adult structures when treated with α-ecdysone or ecdysterone in vitro. Recognizable patterns of the adult fly are not formed though. If metamorphosis of imaginal cell vesicles from in vitro-cultures is induced in vivo by transplantation into host larvae of various ages within the third larval instar, recognizable patterns can differentiate provided the host larva does not metamorphose prior to 2 days after transplantation. The frequency of specific patterns in the implants can be increased by providing 9 days of culture in vivo (adult host flies) before metamorphosis. Passage through the third larval instar is not essential for these cells to produce identifiable patterns since culture in adult flies alone can achieve this. The quality of the differentiated pattern is not correlated with the extent of cell proliferation in the cultured tissues. The problem of pattern specification in vitro and in vivo is discussed.

scholarly journals Genetic localization of a regulatory site necessary for the production of the glue protein P5 in Drosophila melanogaster

1987 ◽  
Vol 49 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Deborah K. Hoshizaki ◽  
Bonnie M. Dlott ◽  
Geoffrey L. Joslyn ◽  
Steven K. Beckendorf
Keyword(s):  
Salivary Gland ◽  
Protein Gene ◽  
Larval Instar ◽  
Major Protein ◽  
Developmentally Regulated ◽  
The Third ◽  
Glue Proteins ◽  
Glue Protein ◽  
The Relationship

SummaryThe glue proteins are products of a developmentally regulated gene family. These genes are transcriptionally active during the third larval instar and code for the major protein products of salivary glands. The activity of several of the genes can be visualized as intermoult puffs in the polytene salivary gland chromosomes. The amount of one of these proteins, P5, varies widely among wild-type strains. We have used biochemical and genetic methods to investigate the source of this variation. The results of in vitro translation of salivary gland RNA suggest that the variation occurs pretranslationally. Genetic mapping experiments showed that sites on several chromosomes can modulate the amount of P5, but that one site on the third chromosome determines the absence and presence of this protein. We have mapped this glue protein gene, called GP5, to the interval between bx (3–58·8) and sr (3–62·0) which also includes the intermoult puff at 90BC. We discuss the relationship between P5 and the glue protein gene Sgs-5 which is also located at 90BC.

Photoperiodism in Orthopodomyia signifera

1973 ◽  
Vol 51 (3) ◽  
pp. 355-357 ◽  
Author(s):  
William E. Bradshaw
Keyword(s):  
North Carolina ◽  
Rapid Development ◽  
Larval Instar ◽  
Developmental Rate ◽  
Photoperiodic Response ◽  
The Third ◽  
Tree Hole ◽  
Fourth Larval Instar ◽  
Lower Temperature ◽  
Short Days

Larvae of the tree-hole mosquito, Orthopodomyia signifera, were collected from North Carolina and subjected to long- and short-day photoperiods. Long days at 25 °C permit rapid molting of fourth instar larvae to pupae. Lower temperature (15 °C) modifies developmental rate but does not appear to block photoperiodic response to long days. Short days at 25 °C may halt development, may retard development, or may permit rapid development in either the third or fourth larval instar. O. signifera is probably polymorphic for both the stage at which diapause may occur and for the depth or firmness with which it is established.

scholarly journals Comparing the Effects of Ethyl Elcoholic and Aquatic Extracts and Alkaline Compounds of Some Plants on the Bioefficacy of Culex pipiens (Diptera:Culicidae)

2021 ◽  
pp. 3350-3357
Author(s):  
Rabah Hassan Saady ◽  
Abdulrahman Jihad Mansoor
Keyword(s):  
Aqueous Extract ◽  
Culex Pipiens ◽  
Biological Effects ◽  
Larval Instar ◽  
Alcoholic Extract ◽  
Killing Rate ◽  
The Third ◽  
Capparis Spinosa

       The results of the present study show the biological effects of ethyl elcoholic and aquatic extracts as well as alkaloids compounds  of Atriplex helmius and Capparis spinosa  roots. The results demonstrated the superiority of the cold aqueous extract of C. capparis at the highest concentration of 20 mg / ml, as the killing rate was 81.16% for the second  larval instar of Cx. pipiens after 72 hours of exposure. While, the killing rate caused by the same treatment was 79.52% for the third larval instar. The results also showed the superiority of the alcoholic extract against C.  spinosa , as the killing rate reached 98.55% for the second larval instar after 72 hours of treatment at a concentration of 20 mg/ml. As for A. halmius extract, the same treatment caused the lowest killing rate of 89.27% ​​ for the third instar of Cx. pipiens. The study also showed significant effects of the raw alkaline compounds’ extracts of A. halimus  roots on the killing of non-adult instars of Cx. pipiens L. The highest rate of mortality observed was 88.54% at the highest concentration of 20 mg /ml at the second instar. Also, significant effects of raw alkaline compounds’ extracts of C. spinosa roots were observed. The highest mortality was 69.21% at the highest concentration of 20 mg/ml for the third larval instar,                                                         

An in vitro Evaluation of the Prevention of Caries on Overdenture Abutments

1988 ◽  
Vol 67 (10) ◽  
pp. 1338-1341 ◽  
Author(s):  
R.L. Ettinger ◽  
D. Manderson ◽  
J.S. Wefel ◽  
M.E. Jensen
Keyword(s):  
Fluoride Concentration ◽  
Root Surface ◽  
Preventive Effect ◽  
The Third ◽  
Anterior Teeth ◽  
High Fluoride ◽  
Time Periods ◽  
Caries Lesions ◽  
Root Surfaces

Teeth prepared as overdenture abutments are susceptible to caries, and it has been shown that brushing by itself is not sufficient to prevent this process. This study evaluated the preventive effect of a remineralization gel which has a low fluoride concentration and compared its effects with those of a phosphate fluoride gel (Karigel), which has a much higher concentration of fluoride. Twenty extracted anterior teeth from patients aged 50 to 70 years were prepared as for overdenture abutments. Each tooth was sectioned into three fragments. An acidified gel system was used to produce artificial caries lesions on the occlusal and root surfaces of each fragment. One fragment of each tooth was treated with the remineralizing gel, the second fragment with a high-fluoride gel, and the third fragment served as the control. Ten teeth were removed at two weeks and again at four weeks, and were sectioned and prepared for histological examination. The depth of the lesions was measured from standardized photomicrographs by means of a sonic digitizer. The conclusions were: (1) Lesions on the occlusal tended to be deeper than those on the root surfaces at four weeks but not at two weeks; and (2) the high-fluoride gel was more protective than the low-fluoride remineralizing solution at both time periods on the occlusal but not on the root surface.

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

1976 ◽  
Vol 35 (02) ◽  
pp. 350-357 ◽  
Author(s):  
Hana Bessler ◽  
Galila Agam ◽  
Meir Djaldetti
Keyword(s):  
Protein Synthesis ◽  
Fold Increase ◽  
Human Platelets ◽  
Polystyrene Latex ◽  
Latex Particles ◽  
The Third ◽  
Platelet Protein ◽  
Dose Dependent ◽  
Phagocytotic Activity

SummaryA three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

Studies on Thrombolysis with Streptokinase

1971 ◽  
Vol 25 (02) ◽  
pp. 354-378 ◽  
Author(s):  
R Gottlob ◽  
L Stockinger ◽  
U Pötting ◽  
G Schattenmann
Keyword(s):  
Electron Microscopy ◽  
Cross Section ◽  
Whole Blood ◽  
Jugular Vein ◽  
Thin Sections ◽  
The Third ◽  
Morphological Findings ◽  
Blood Clots ◽  
Arteries And Veins

SummaryIn vitro whole blood clots of various ages, experimental thrombi produced in the jugular vein of rabbits and human thrombi from arteries and veins were examined in semi-thin sections and by means of electron microscopy.In all types of clots examined a typical course of retraction was found. Retraction starts with a dense excentrical focus which grows into a densification ring. After 24 hours the entire clot becomes almost homogeneously dense; later a secondary swelling sets in.Shortly after coagulation the erythrocytes on the rim of the clot are bi-concave discs. They then assume the shape of crenate spheres, turn into smooth spheres and finally become indented ghosts which have lost the largest part of their contents. In the inner zone, which makes up the bulk of the clot, we observed bi-concave discs prior to retraction. After retraction we see no crenations but irregularly shaped erythrocytes. Once the secondary swelling sets in, the cross-section becomes polygonal and later spherical. After extensive hemolysis we observe the “retiform thrombus” made up of ghosts.Experimental and clinical thrombi present the same morphology but are differentiated from in vitro clots by: earlier hemolysis, immigration of leukocytes, formation of a rim layer consisting of fibrin and thrombocytes, and the symptoms of organization. Such symptoms of organization which definitely will prevent lysis with streptokinase were found relatively late in experimental and clinical thrombi. Capillary buds and capillary loops were never found in clinical thrombi prior to the third month.The morphological findings agree with earlier physical and enzymatic investigations. The observation that phenomena of reorganization occur relatively late and frequently only in the rim areas of large thrombi explains why lytic therapy is possible in some of the chronic obliterations.

CONDITIONS FAVOURABLE FOR PROTECTION THE MARINE SHORE OF THE VISTULA SPIT AND SAMBIAN PENINSULA (THE BATIC SEA, KALININGRAD OBLAST) BY OFFSHORE DISPOSAL OF DREDGED MATERIAL

2017 ◽  
Author(s):  
Andrei Sokolov ◽  
Andrei Sokolov ◽  
Boris Chubarenko ◽  
Boris Chubarenko
Keyword(s):  
Dredged Material ◽  
Vistula Lagoon ◽  
Shore Protection ◽  
The South ◽  
The Third ◽  
Dumping Site ◽  
The North ◽  
Kaliningrad Oblast ◽  
Wind Actions ◽  
The Vistula Lagoon

Three dumping sites located at the south-eastern part of the Baltic Sea (Kaliningrad Oblast) at shallow depths are considered. The first one is located to the south of the Vistula Lagoon inlet in front of a permanently eroded open marine shore segment. The second one is located to the north of the Vistula Lagoon inlet, and is used now for disposing of dredged material extracted from the Kaliningrad Seaway Canal. The third dumping site is located near the northern shore of the Sambian Peninsula to the east of the Cape Gvardeijski and assigned for disposing the dredged material extracted from the fairway to the Pionerskij Port located nearby. The last site is planned to be used for disposing of dredged material from the future port that should be constructed there before the beginning of the FIFA World Cup 2018. All three dumping sites are located not far from the eroded segments of the shore. The question behind the study is: would it possible that disposed material will naturally transported from the damping site to the shore and accumulate there to protect it from erosion? A numerical hydrodynamic-transport 3D model (MIKE) was used to model sediment transport under different wind actions. The winds with the speed stronger than 15 m/s complete wash out disposed material from the dumping site and spreading it over the wide area with a negligible layer thickness. Winds of about 7-10 m/s transport material along the shore at a distance of few kilometers that may be useful for shore protection. The first location of the dumping site (to the south of the Vistula Lagoon inlet) looks very ineffective for potential protection the shore nearby. At the other hand, the second and especially the third locations are favorable for transport of disposed material to the shore, the most favorable conditions are at onshore or alongshore currents.

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