Heavy metal (As, Cd, Cr, Hg, Pb) analysis and identification of heavy metal resistant bacteria in sediments from Manado Bay

Abstract Environmental pollution from heavy metals is becoming a growing concern due to the adverse effects it is causing throughout the world. This study aims to analyze heavy metal concentrations and identify heavy metal resistant bacteria in the bay of Manado. Sediment samples were collected from five bays in Manado. The concentrations of heavy metals As, Cd, Cr, Hg and Pb were analyzed using ICP-OES, and Hg using CV-AFS. Bacteria from the sediment were grown in nutrient broth media containing heavy metals As, Cd, Cr, Hg and Pb respectively. Microbiology and 16SrRNA gene analysis were used to identify the bacteria that grown on media containing varying concentrations of heavy metals. The results showed that the sediments from the five bays in Manado contained heavy metals with an average concentration of As <1mg/kg, Cd 1.8mg/kg), Cr 6.2mg/kg, Hg <0.07mg/kg). and Pb 11.2mg/kg. The results of microbiological and molecular analysis showed that 5 species of heavy metal resistant bacteria were Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus arlettae, Acinobacter sp., and Brevibacterium sp. The five bacteria found to be resistant to heavy metals can be used to detoxify As, Cd, Cr, Hg, and Pb.

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Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

International Journal of Environment ◽

10.3126/ije.v7i1.21292 ◽

2018 ◽

Vol 7 (1) ◽

pp. 28-40

Author(s):

Bikram Gautam ◽

Rameshwar Adhikari

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Antibiotic Resistance ◽

Treatment Plant ◽

Resistance Pattern ◽

Resistant Bacteria ◽

Grab Sampling ◽

Antibiotic Resistance Pattern ◽

Antibiotic Susceptibility Test ◽

Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized.

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Distribution and Potential Risk of Heavy Metals in Sediments of the Three Gorges Reservoir: The Relationship to Environmental Variables

Water ◽

10.3390/w10121840 ◽

2018 ◽

Vol 10 (12) ◽

pp. 1840 ◽

Cited By ~ 6

Author(s):

Lei Huang ◽

Hongwei Fang ◽

Ke Ni ◽

Wenjun Yang ◽

Weihua Zhao ◽

...

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Environmental Variables ◽

Three Gorges Reservoir ◽

Three Gorges ◽

Metal Concentrations ◽

Sediment Samples ◽

Heavy Metal Concentrations ◽

Sediment Size ◽

The Three Gorges

In this study, surface sediment samples were taken from the Three Gorges Reservoir (TGR) in June 2015 to estimate the spatial distribution and potential risk of Cu, Zn, Cd, Pb, Cr, and Ni (34 sites from the mainstream and 9 sites from the major tributaries), and correlations with environmental variables were analyzed (e.g., median sediment size, water depth, turbidity, dissolved oxygen of the bottom water samples, and total organic carbon, total nitrogen, and total phosphorus of the surface sediment samples). Results show that the heavy metal concentrations in the sediments have increased over the last few decades, especially for Cd and Pb; and the sites in the downstream area, e.g., Badong (BD) and Wushan (WS), have had greater increments of heavy metal concentrations. The sampling sites from S6 to S12-WS are identified as hot spots for heavy metal distribution and have relatively high heavy metal concentrations, and there are also high values for the sites affected by urban cities (e.g., the concentrations of Zn, Cd, Cr and Ni for the site S12-WS). Overall, the heavy metal concentrations increased slightly along the mainstream due to pollutants discharged along the Yangtze River and sediment sorting in the reservoir, and the values in the mainstream were greater than those in the tributaries. Meanwhile, the heavy metal concentrations were generally positively correlated with water depth (especially for Ni), while negatively correlated with dissolved oxygen, turbidity, and median sediment size. These environmental variables have a great impact on the partition of heavy metals between the sediment and overlying water. According to the risk assessment, the heavy metals in the surface sediments of TGR give a low to moderate level of pollution.

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Evaluation of the accumulation of sediment and heavy metals in a storm-water detention pond

Water Science & Technology ◽

10.2166/wst.2002.0122 ◽

2002 ◽

Vol 45 (7) ◽

pp. 105-112 ◽

Cited By ~ 25

Author(s):

C. Färm

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Metal Content ◽

Reduction Rate ◽

Storm Water ◽

Total N ◽

Sediment Samples ◽

Heavy Metal Concentrations ◽

Detention Pond ◽

Total P

Accumulation of sediments and water quality were investigated in a wet storm-water detention pond in central Sweden. The drainage area surrounding the pond was covered by a section of highway and secondary roads. Sediment samples were collected from the pond and heavy-metal concentrations analysed subsequently in the laboratory. The depth of accumulated sediments was measured at several points around the pond. The investigation showed that during the 18 months that had passed since the pond was constructed, a 5–8 cm layer of sediments had accumulated near the inlet, and a 1.5 cm layer near the outlet of the pond. Storm water passing through the detention pond showed an average reduction rate of 26–84% for total metal content, 67% for total N, 78% for total P and 92% for COD.

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Heavy Metal Pollution Characteristics in the Sediment of Elephant Lake in Nanchang City, Jiangxi

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.448-453.380 ◽

2013 ◽

Vol 448-453 ◽

pp. 380-384

Author(s):

Yue Liang ◽

Wen Hua Li ◽

Xiao Zhen Liu ◽

Jian Xiong

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Microwave Digestion ◽

Quality Standards ◽

Icp Oes ◽

Sediment Samples ◽

Bcr Method ◽

Cd Contamination ◽

Metal Contents ◽

Pre Treatment

In order to understand the pollution features of heavy metals in sediments of Elephant Lake in Nanchang city, sediment samples were collected and detected .Using microwave digestion and BCR Method for sediment pre-treatment, the concentrations of heavy elements were measured by ICP-OES emission spectrometer. Sediment heavy metal contents were in descending order of Zn> Pb> Cu> Cr> As> Cd. According to the national soil environmental quality standards of P.R.China, there was Cd contamination in lakebed mud. And five kinds of metals existed mainly in oxidizable (F3) and residual (F4) , indicating less risk of migration of heavy metals .

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Diversity and Distribution of Heavy Metal-Resistant Bacteria in Polluted Sediments of the Araça Bay, São Sebastião (SP), and the Relationship Between Heavy Metals and Organic Matter Concentrations

Microbial Ecology ◽

10.1007/s00248-016-0821-x ◽

2016 ◽

Vol 72 (3) ◽

pp. 582-594 ◽

Cited By ~ 21

Author(s):

Bruna Del Busso Zampieri ◽

Aline Bartelochi Pinto ◽

Leonardo Schultz ◽

Marcos Antonio de Oliveira ◽

Ana Julia Fernandes Cardoso de Oliveira

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Organic Matter ◽

Resistant Bacteria ◽

Polluted Sediments ◽

Heavy Metal Resistant ◽

The Relationship

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Measurement and ecological risk assessment of heavy metals accumulated in sediment and water collected from Gomishan international wetland, Iran

Water Science & Technology ◽

10.2166/wst.2021.317 ◽

2021 ◽

Vol 84 (6) ◽

pp. 1498-1508

Author(s):

Nazanin Kalani ◽

Borhan Riazi ◽

Abdolreza Karbassi ◽

Faramarz Moattar

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Ecological Risk ◽

Metal Contamination ◽

Heavy Metal Contamination ◽

Moderate Risk ◽

Sediment Samples ◽

Heavy Metal Concentrations ◽

Water And Sediment ◽

Icp Ms

Abstract This study aimed to measure and ecologically assess heavy metals, including As, Cr, Pb, Cd, and Ni in water and sediment samples taken from Gomishan, an international wetland located in Golestan, Iran. Four sampling stations were selected to cover all parts of the wetland. The analyses of the heavy metals were performed by ICP-MS. Based on the content of the heavy metals in the sediments, the values of risks for individual heavy metals, as Er, and for total heavy metals, as IR, were estimated. Igeo and EF also presented the soil quality in terms of accumulated contamination. The average content of the heavy metals in water was 23.12, 4.14, 10.04, 6.71, and 94.48 μg/L for As, Cd, Cr, Ni, and Pb, respectively. The heavy metal concentrations in sediments were decreased in the following order: Pb (2130 ppb) > As (655 ppb) > Cr (295 ppb) > Ni (148.8 ppb) > Cd (148.8 ppb). The potential risk values for individual heavy metals were in the low range, Er < 40, except for Cd, which mostly posed a moderate ecological risk. The values of EF and Igeo showed that the sediments sampled from the Gomishan wetland were minimally enriched and contaminated. As the Gomishan wetland has a moderate risk of heavy metal contamination, conservative and monitoring activities should be performed.

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Heavy metal concentrations in zooplankton of Sinop coasts of the Black Sea, Turkey

Marine Biological Journal ◽

10.21072/mbj.2016.01.1.01 ◽

2016 ◽

Vol 1 (1) ◽

pp. 5-13 ◽

Cited By ~ 2

Author(s):

L. Bat ◽

F. Üstün ◽

H. C. Öztekin

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Detection Limit ◽

Black Sea ◽

Mesh Size ◽

Average Concentration ◽

The Other ◽

The Black Sea ◽

Total Zooplankton ◽

Heavy Metal Concentrations

Estimation of level contamination of marine ecosystems with heavy metals is important task in the field of MSFD. Total zooplankton samples were collected using a standard zooplankton net (mesh size = 112 µm, mouth diameter = 50 cm) from Sinop coast of the Black Sea, Turkey in 2011 and 2012. Concentrations of Al, As, Cu, Zn, Hg, Fe, Cd and Pb were determined in the total zooplankton samples. The average concentration of heavy metals followed order: Fe > Al > Zn > Pb > Cu > As > Cd > Hg. Of all the heavy metals estimated Hg was below the detection limit in all the samples. Fe was the most common heavy metal in total zooplankton samples present in all, Al was the other heavy metal commonly present in the samples. Pb showed high value (210 mg·kg-1) in total zooplankton. Similar to Pb, a high average concentration of As (40 mg·kg-1) was observed in total zooplankton. Cd was 1.1 mg·kg-1.

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Isolation and screening of heavy metal resistant bacteria from wastewater: a study of heavy metal co-resistance and antibiotics resistance

Water Science & Technology ◽

10.2166/wst.2012.355 ◽

2012 ◽

Vol 66 (10) ◽

pp. 2041-2048 ◽

Cited By ~ 10

Author(s):

Benmalek Yamina ◽

Benayad Tahar ◽

Fardeau Marie Laure

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Vibrio Fischeri ◽

Micrococcus Luteus ◽

Toxicity Tests ◽

Resistant Bacteria ◽

Antibiotics Resistance ◽

Resistant Species ◽

Luminescence Inhibition ◽

Heavy Metal Resistant

The uncontrolled discharges of wastes containing a large quantity of heavy metal create huge economical and healthcare burdens particularly for people living near that area. However, the bioremediation of metal pollutants from wastewater using metal-resistant bacteria is a very important aspect of environmental biotechnology. In this study, 13 heavy metal resistant bacteria were isolated from the wastewater of wadi El Harrach in the east of Algiers and characterized. These include zinc-, lead-, chromium- and cadmium-resistant bacteria. The metal-resistant isolates characterized include both Gram-negative (77%) and Gram-positive (23%) bacteria. The Minimum Inhibitory Concentration (MIC) of wastewater isolates against the four heavy metals was determined in solid media and ranged from 100 to 1,500 μg/ml. All the isolates showed co-resistance to other heavy metals and antibiotic resistance of which 15% were resistant to one antibiotic and 85% were multi- and bi-antibiotics resistant. The zinc-resistant species Micrococcus luteus was the much more heavy metal resistant. The results of toxicity tests on Vibrio fischeri showed that the DI50 (5 min) as low as 0.1 carried away luminescence inhibition greater than 50%.

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Mapping seasonal variation in the distribution and concentration of heavy metals using water quality index and geographic information system based kriging

Journal of Geographical Research ◽

10.30564/jgr.v4i2.3100 ◽

2021 ◽

Vol 4 (2) ◽

Author(s):

Kofi Adu-Boahen ◽

Isaac. Boateng ◽

Ishmael. Yaw. Dadson ◽

Laud. Alfred. Dei ◽

Kwabena. Barima. Antwi. ◽

...

Keyword(s):

Heavy Metals ◽

Water Quality ◽

Heavy Metal ◽

Water Quality Index ◽

Quality Index ◽

World Health ◽

Heavy Metal Concentrations ◽

The World ◽

Dry Seasons ◽

Wet And Dry Seasons

Incessant monitoring of water is essential in terms of heavy metals and toxic substances as it provides detailed information on aquatic resources. Majority of lagoons receive freshwater from their catchment areas containing industrial and domestic waste. The paper analysed seasonal variations in the distribution and concentrations of Lead (Pb), Copper (Cu), Cadmium (Cd), and Manganese (Mn) in the Fosu lagoon in Ghana to ascertain the quality of the lagoon. Water was sampled from eighteen (18) different points on the lagoon and was analysed at the Water Research Institute (WRI) of the Center for Scientific and Industrial Research (CSIR) using Atomic Absorption Spectrometry (AAS) and the results were interpolated using kriging. The results obtained were compared with the World Health Organisation water quality index. Statistical analysis of heavy metal concentrations using Pearson’s two-tailed significance correlation showed positive correlations for both seasons; between Pb and Cu (0.297; sig. = 0.232, and 0.196; sig. = 0.436), and Cd and Mn (0.119; sig. = 0.643 and 0.191; sig. = 0.447) for the wet and dry seasons respectively. A paired sample t-test on concentrations also showed statistical differences between wet and dry seasons’ concentrations for Pb (t = 1.324; sig. = 0.203), Cu (t = 2.759; sig. = 0.013), and Cd (t = 3.056; sig. = 0.007), and Mn (t = -4.014; sig. = 0.001). Pb and Cd showed higher concentrations above the World Health Organisation's permissible limits. Heavy metal concentrations of water samples analysed varied widely in terms of seasons and sampling points.

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Study on Heavy Metal Resistant Bacteria in Guheswori Sewage Treatment Plant

Our Nature ◽

10.3126/on.v6i1.1655 ◽

2009 ◽

Vol 6 (1) ◽

pp. 52-57 ◽

Cited By ~ 42

Author(s):

A. Rajbanshi

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Antibiotic Resistance ◽

Sewage Treatment ◽

Sewage Treatment Plant ◽

Treatment Plant ◽

Resistant Bacteria ◽

Heavy Metal Resistant ◽

Staphylococcus Spp ◽

Multiple Tolerance

Removal of heavy metals from wastewater needs advance chemical technology and is more expensive too. The cheaper alternative for this is the bioremediation using heavy metals resistant microorganisms. In this study, 10 heavy metal resistant bacteria were isolated from oxidation ditch of wastewater treatment plant of Bagmati Area Sewerage Project. These include chromium resistant Staphylococcus spp, Escherichia coli, Klebsiella spp; cadmium resistant Acinetobacter spp, Flavobacterium spp, Citrobacter spp; nickel resistant Staphylococcus spp, Bacillus spp; copper resistant Pseudomonas spp; and cobalt resistant Methylobacterium spp. All the isolates showed high resistance to heavy metals with Minimum Inhibitor Concentration (MIC) for heavy metals ranging from 150 mug/ml to 500 mug/ml. Six resistant isolates showed multiple tolerance to heavy metals. All the 10 isolates also showed antibiotic resistance of which 10% were resistant to single antibiotic and 90% were multi-antibiotic resistant. Heavy metal tolerance test showed maximum microbial tolerance to chromium and minimum tolerance to nickel in mixed liquor sample of oxidation ditch.Keywords: Heavy metal resistant bacteria, multiple tolerance, antibiotic resistance, Guheswori Sewage Treatment Plantdoi: 10.3126/on.v6i1.1655Our Nature (2008)6:52-57

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