Anti-bacterial Activity of Alginate Based Edible Coating Solution Added with Lemongrass Essential Oil Against Some Pathogenic Bacteria

Abstract Edible coating is a thin layer that can be made of several materials, one of which is polysaccharides, such as alginates, carrageenan, agar, chitin, chitosan, pectin, starch, cellulose derivatives, and gums. The application of food products with edible coatings has been widely carried out and has been proven to improve quality and extend the shelf life of the product. Antibacterial agent can be added to improve the performance of edible coating. This study was aimed to explore the potential of lemongrass essential oil as an antibacterial agent in alginate based antibacterial edible coating solutions, especially against pathogenic bacteria. Edible coating is made of alginate powder, modified cassava flour (Mocaf), beeswax, glycerol and antibacterial agent. The additional levels of lemongrass essential oil into edible coating formula were 0.25, 1.50 and 0.75%. The antibacterial activity of the edible coating solution produced was tested against Escherichia coli, Staphylococcus aureus, Salmonella, and Pseudomonas aeruginosa by employing a disk diffusion testing method. Antibacterial agents used in previous studies to develop alginate-based antibacterial edible coatings and potential applications of the developed alginate-based edible coatings incorporated with lemongrass essential oil were identified by browsing through the internet. The results showed that various antibacterial agents have been explored to improve the performance of alginate based edible coatings. An antibacterial activity study exhibited that the highest antibacterial activity was found in the edible coating with the addition of 0.50% lemongrass essential oil, except for S. aureus at the addition level of 0.75%. This study suggests that the addition of lemongrass essential oil for the manufacture of alginate based edible coating solution is 0.50%. The potential uses of edible coatings made from alginate with the addition of lemongrass essential oil are to coat various animal and plant based food products.

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Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

Baghdad Science Journal ◽

10.21123/bsj.7.3.1159-1165 ◽

2010 ◽

Vol 7 (3) ◽

pp. 1159-1165

Author(s):

Baghdad Science Journal

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Cold Water ◽

Water Extract ◽

Ethanolic Extract ◽

Hot Water ◽

Diffusion Method ◽

Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

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Antimicrobial Applications of Nanoparticles

10.4018/978-1-7998-8936-6.ch012 ◽

2022 ◽

pp. 269-288

Author(s):

Ayesha Kanwal ◽

Zeeshan Ahmad Bhutta ◽

Ambreen Ashar ◽

Ashar Mahfooz ◽

Rizwan Ahmed ◽

...

Keyword(s):

Antibacterial Activity ◽

Side Effects ◽

Antibacterial Agent ◽

Antibacterial Agents ◽

The Other ◽

Drug Resistant ◽

Short Term ◽

Human Mortality ◽

Recent Developments

Human mortality due to drug-resistant infections is becoming more prevalent in our society. Antibiotics are impotent due to abuse and/or misuse, leading to new, more expensive, and more effective medicines and treatments. Therefore, it causes many short-term and long-term side effects in the patient. On the other hand, nanoparticles have exhibited antibacterial activity against various pathogens due to their small size and ability to destroy cells by various mechanisms. Unlike antibiotics for the treatment of patients' diseases and infections, nanomaterials provide an exciting way to limit the growth of microorganisms due to infections in humans. This has led to the development of a number of nanoparticles as active antibacterial agents. Therefore, the authors have carefully reviewed the recent developments in the use of nanomaterials for antibacterial applications and the mechanisms that make them an effective alternate antibacterial agent.

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Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

Natural Product Communications ◽

10.1177/1934578x20953252 ◽

2020 ◽

Vol 15 (9) ◽

pp. 1934578X2095325

Author(s):

Bala Namata Abba ◽

Abderrahmane Romane ◽

Amadou Tidjani Ilagouma

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Traditional Medicine ◽

Pathogenic Bacteria ◽

Ethanolic Extract ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

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Fabrication of Antimicrobial Perspiration Pads and Cotton Cloth UsingAmaranthus dubiusMediated Silver Nanoparticles

Journal of Chemistry ◽

10.1155/2013/741743 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 7

Author(s):

M. Jannathul Firdhouse ◽

P. Lalitha

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Medical Devices ◽

Food Industry ◽

Pathogenic Bacteria ◽

Antibacterial Agents ◽

Cotton Cloth ◽

Textile Materials ◽

Textile Fabrics ◽

Antibacterial Textile

Silver nanoparticles prepared through a simplistic method using the aqueous extract ofAmaranthus dubiuswere fabricated on perspiration pads and cotton cloth samples to obtain antibacterial textile materials by two different fabrication methods. The antibacterial activity was investigated against the bacteriaCorynebacteriumwhich is commonly present in sweat. Silver nanoparticles that serve as antibacterial agents, against pathogenic bacteria, have gained increased applications in medical devices, textile fabrics, and food industry and hence the result of this study would be a welcomed option.

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Determination of antibacterial activity of vacuum distillation fractions of lemongrass essential oil

Phytochemistry Reviews ◽

10.1007/s11101-012-9255-3 ◽

2012 ◽

Vol 11 (4) ◽

pp. 405-412 ◽

Cited By ~ 12

Author(s):

Manuel A. Falcão ◽

Ana L. B. Fianco ◽

Aline M. Lucas ◽

Marcos A. A. Pereira ◽

Fernando C. Torres ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Vacuum Distillation ◽

Lemongrass Essential Oil

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Antimicrobial Activity of Lemongrass Essential Oil (Cymbopogon flexuosus) and Its Active Component Citral Against Dual-Species Biofilms of Staphylococcus aureus and Candida Species

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.603858 ◽

2020 ◽

Vol 10 ◽

Author(s):

Shanjun Gao ◽

Guangzhi Liu ◽

Jianguo Li ◽

Jing Chen ◽

Lina Li ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Expression Of Genes ◽

Cymbopogon Flexuosus ◽

Underlying Mechanisms ◽

Bacteria And Fungi ◽

Conventional Antibiotics ◽

Lemongrass Essential Oil

Compared to mono-species biofilm, biofilms formed by cross-kingdom pathogens are more refractory to conventional antibiotics, thus complicating clinical treatment and causing significant morbidity. Lemongrass essential oil and its bioactive component citral were previously demonstrated to possess strong antimicrobial efficacy against pathogenic bacteria and fungi. However, their effects on polymicrobial biofilms remain to be determined. In this study, the efficacy of lemongrass (Cymbopogon flexuosus) essential oil and its bioactive part citral against dual-species biofilms formed by Staphylococcus aureus and Candida species was evaluated in vitro. Biofilm staining and viability test showed both lemongrass essential oil and citral were able to reduce biofilm biomass and cell viability of each species in the biofilm. Microscopic examinations showed these agents interfered with adhesive characteristics of each species and disrupted biofilm matrix through counteracting nucleic acids, proteins and carbohydrates in the biofilm. Moreover, transcriptional analyses indicated citral downregulated hyphal adhesins and virulent factors of Candida albicans, while also reducing expression of genes involved in quorum sensing, peptidoglycan and fatty acids biosynthesis of S. aureus. Taken together, our results demonstrate the potential of lemongrass essential oil and citral as promising agents against polymicrobial biofilms as well as the underlying mechanisms of their activity in this setting.

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Synthesis and in vitro Antibacterial Activity of Quaternization 10-Methoxycanthin-6-one Derivatives

10.20944/preprints201902.0248.v1 ◽

2019 ◽

Author(s):

Na Li ◽

Jiang-Kun Dai ◽

Dan Liu ◽

Jin-Yi Wang ◽

Jun-Ru Wang

Keyword(s):

Antibacterial Activity ◽

Natural Products ◽

Minimum Inhibitory Concentration ◽

Pathogenic Bacteria ◽

Antibacterial Agents ◽

Inhibitory Concentration ◽

Dilution Method ◽

In Vitro Antibacterial Activity

Natural products are an important source of antibacterial agents. Canthin-6-one alkaloids have displayed potential antibacterial activity based on our previous work. In order to improve the activity, twenty-two new 3-N-benzylated 10-methoxy canthin-6-ones were designed and synthesized through quaternization reaction. The in vitro antibacterial activity against three bacteria was evaluated by double dilution method. Four compounds (6f, 6i, 6p and 6t) displayed 2-fold superiority (minimum inhibitory concentration (MIC) = 3.91 µg/mL) against agricultural pathogenic bacteria R. solanacearum and P. syringae than agrochemical propineb. Moreover, the structure–activity relationships (SARs) were also carefully summarized in order to guide the development of antibacterial canthin-6-one agents.

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Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae

Current Biochemistry ◽

10.29244/cb.vol7.iss1.a1 ◽

2020 ◽

Vol 7 (1) ◽

pp. 1-9

Author(s):

Nuke Annisa Nasution ◽

I Made Artika ◽

Dodi Safari

Keyword(s):

Antibacterial Activity ◽

Streptococcus Pneumoniae ◽

Clinical Isolate ◽

Antibacterial Agent ◽

Antibacterial Agents ◽

Leaf Extracts ◽

Extract Concentration ◽

Global Concern ◽

Muntingia Calabura

Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern. The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae. Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol. The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067. Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%. In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%. Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.

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IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s5.t0084 ◽

2019 ◽

pp. 113-115

Author(s):

PURIT PATTANAPANIT ◽

SUNISA MITHONGLANG ◽

SUNITA MITHONGLANG ◽

SURACHAI TECHAOEI

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Aromatic Plants ◽

Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

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