scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

Author(s):  
PURIT PATTANAPANIT ◽  
SUNISA MITHONGLANG ◽  
SUNITA MITHONGLANG ◽  
SURACHAI TECHAOEI

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds


Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.


Author(s):  
B. R. Malathy ◽  
Sweetlin Ajitha P ◽  
Sangeetha K. S ◽  
Swetha Thampy ◽  
Kamala G

Essential oils (EOs) are natural extracts from the seeds, stems, roots, flowers, bark and other parts of the plant prepared by steam distillation. They are complex, volatile, natural compounds formed by aromatic plants as secondary metabolites. They are known for their bactericidal, virucidal, fungicidal, sedative, anti-inflammatory, analgesic, spasmolytic and locally anesthetic properties. They are generally composed of a combination of substances like terpenes, phenolics, aldehydes or alcohols. The complex composition and different mechanisms of action of EOs may be an advantage over other antimicrobials to prevent the development of resistance of pathogenic bacteria. With this background, the aim of this study was to evaluate the antimicrobial activity of five essential oils like basil, lime, rosemary, thyme and canada balsam against 14 microbes. The effects of essential oil on the selected microbes were determined by agar well diffusion method. The zone of inhibition was observed and measured in millimeter. Essential oils which showed inhibitory diameter >15 mm were further tested to determine the minimum inhibitory concentration (MIC). S. aureus, E. coli, S. mutans, S. sanguinis, C. albicans and M. furfur were inhibited by all essential oils. K. pneumoniae, P. aeruginosa and E .faecalis were inhibited only by thyme and not by other essential oils. The MIC values ranged from 50% to 0.10%. The least MIC value of 0.10% was shown by thyme and basil to S. aureus, thyme to E.coli and all essential oils against C. albicans except lime.


2017 ◽  
Vol 2 (6) ◽  
pp. 563 ◽  
Author(s):  
Sarifah Nurjanah ◽  
Indira Lanti Putri ◽  
Dwi Pretti Sugiarti

Indonesia is one of the largest producer of nutmeg oil  (Myristica fragrans). This essential oil has a lot of usefulness for food and pharmaceutical industries, however antibacterial activity of Indonesian nutmeg oil  has not been investigated yet.  Antibacterial activity   Myristica fragrans oil from two areas respectively (Sulawesi and Central Java) were investigated.  The essential oils was extracted using water and steam distiller and then its antibacterial activity against pathogenic bacteria (gram-positive bacteria : Staphylococcus aureus, Staphylococcus epidermis, and gram-negative bacteria :  Shigella Dysenteriae, Salmonella Typhi) was examined.  Resistance pattern was studied by in vitro disc diffusion method   using essential oil concentration   20%, 40%, 60%, 80% and 100%.   The result showed that the two essential oils inhibited all bacteria. The highest inhibition zone on Central Java nutmeg oil was on 60% concentration of the oil (12.96 16.79, 13.46 and 16.50 mm for S. aureus, S. epidermis, S. dysenteriae, S. typhi respectively), while on Sulawesi nutmeg oil was on 100% concentration (18.84, 16.54, 17.84 and 12.54 mm for S. aureus, S. epidermis, S. dysenteriae, S. typhi respectively). Keywords: Antibacterial activity; Nutmeg oil; Central Java; Sulawesi


2019 ◽  
Vol 65 (2) ◽  
pp. 22-29
Author(s):  
Said Oulkheir ◽  
Hadia Boumariem ◽  
Hanane Dand ◽  
Mohamed Aghrouch ◽  
Khadija Ounine ◽  
...  

Summary Introduction: Recently, efforts regarding the discovery of the effectual components of plants possessing antimicrobial properties are advanced. Herbal essential oils are widely used for treatment of various diseases, and they play an important role in healthcare considerations. Objective: This study aims to evaluate the effectiveness of Cinnamomum verum, Eucalyptus globulus, Lavandula angustifolia and Mentha pulegium essential oils against Candida albicans and some pathogenic bacteria. Methods: The antibacterial activity of four essential oils (EOs) against different microbial strains was evaluated using the disk diffusion method as well as determination of the minimal inhibitory concentration (MIC), and bactericidal concentration (MBC). For Candida albicans, the MFC of the plant oils was determined using a macro broth dilution assay. A range of concentrations (50 to 0.2 mg/ml) were prepared in Mueller Hinton Broth medium in flasks. Tween 80 (0.01% v/v) was included to enhance oil solubility. Each flask was inoculated with 108 CFU/ml of C. albicans. The flasks were incubated at 35°C for 48 hours. From each flask 13 μl of culture was inoculated onto Mueller-Hinton Agar plates and incubated at 35°C for 48 h. The plates were observed and the MFC was determined as the lowest concentration of plant oil completely inhibiting the growth of C. albicans. Results: The obtained results showed that all bacteria and yeasts tested were sensitive to cinnamon essential oil with an inhibition zone ranging from 22 to 39.33 mm and a MIC ranging from 0.20 mg/ml to 1.56 mg/ml. At low concentrations ranging from 0.2 to 3.13 mg/ml, this essential oil has shown the most important bactericidal effect. Eucalyptus essential oil showed the highest inhibitory effect on Staphylococcus aureus with a diameter of 21.33±1.15 mm. The antibacterial effect of mint indicates that the most sensitive bacterium is A. boumannii. However, S. enteritidis, C. albicans, K. pneumoni and P. aeruginosa are resistant germs whose inhibition diameter varies from 7.33±1.15 mm to 11.33±1.15 mm. Lavender EO has an inhibitory effect against S. aureus (20.67±1.15 mm) and an intermediate effect against Streptococcus pyogenes, Serratia marcescens and Enterococcus faecalis. Conclusions: The antibacterial activity of essential oils, especially those of cinnamon against the strains studied, supports their potential use as a remedy against infectious microbial diseases.


2019 ◽  
Vol 49 (2) ◽  
Author(s):  
Paula Karoline Soares Farias ◽  
Júlio César Rodrigues Lopes Silva ◽  
Cintya Neves de Souza ◽  
Francine Souza Alves da Fonseca ◽  
Igor Viana Brandi ◽  
...  

ABSTRACT: Studies about preservative and antioxidant activity of essential oils have been encouraged in recent years, given their importance to food industry. The aim of the current study was to evaluate the antioxidant properties and antimicrobial activity of essential oils deriving from Syzygium aromaticum, Cymbopogon citratus and Lippia alba against lactic and pathogenic bacteria responsible for food-borne diseases. Essential oil antibacterial activity was assessed through disc diffusion and macrodilution tests conducted in a mixed lactic culture of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus (YF-L903) and of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538), Salmonella enterica (ATCC 6017) strains. Based on the chromatographic analysis results, the essential oils shown to be composed of eugenol (79.41%) which was the prevalent compound in S. aromaticum, geranial (31.89%), neral (24.52%) and β-myrcene (25.37%) in C. citratus, as well as of geranial (33.80%) and neral (25.63%) in L. alba. The observed antibacterial activity confirmed the dose-dependent effect of these three oils on all the assessed bacteria; there was halo inhibition at concentration 20μL mL-1. The essential oil of S. aromaticum presented better antioxidant activity, with IC50 equal to 5.76μg mL-1 and antioxidant activity index of 6.94, and it was considered strong (AAI>2.0) in comparison to the other evaluated oils. This essential oil also presented excellent antioxidant activity at concentrations lower than the one required to inhibit lactic cultures. Based in this outcome, the essential oil from S. aromaticum can be used as preservative agent in processed food whose formulation presents lactic cultures.


2021 ◽  
pp. 2206-2211
Author(s):  
Elgio Venanda Ginting ◽  
Endah Retnaningrum ◽  
Dyah Ayu Widiasih

Background and Aim: Extended-spectrum β-lactamase (ESBL) is an enzyme produced by the family of Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae, which can hydrolyze β-lactam antibiotics, such as penicillins, cephalosporins, cephamycin, and carbapenem. ESBL-producing bacteria are widely distributed from farms to slaughterhouses until food products originating from animals are available in the market, which plays an important role as a pathway for the exposure and transmission of ESBL-producing bacteria from food products of animal origin to humans. This study aimed to determine the antibacterial activity of Syzygium aromaticum (clove) and Cinnamomum verum (cinnamon) essential oils against strains resistant to ESBL-producing E. coli and K. pneumoniae isolates. Materials and Methods: The antibacterial activity of clove and cinnamon essential oils was tested against three strains of tested bacteria using the disk diffusion method. The minimum inhibitory concentration (MIC) of clove and cinnamon essential oils was determined using the broth microdilution method. The minimum bactericidal concentration (MBC) was determined using the MIC. Morphological changes on each tested bacteria were observed through scanning electron microscopy (SEM). Results: Both essential oils exhibited inhibitory effects toward all test organisms, indicated by inhibition zones around the disk. The MIC values of clove essential oil were 0.078% (v/v) for all tested bacteria, whereas the MICs of cinnamon essential oil ranged from 0.039% (v/v) to 0.156% (v/v) for all tested bacteria. MBC values of clove and cinnamon essential oils ranged from 0.078% (v/v) to 0.156% (v/v) for all tested bacteria. There were morphological changes in each tested bacterial cell that was observed through SEM. Each tested bacteria treated with clove and cinnamon essential oils showed shrinkage and cells lysis. Conclusion: It was concluded that clove and cinnamon essential oils have emerged as effective antibacterial agents by showing high antibacterial activity against ESBL-producing E. coli and K. pneumoniae isolates, as evidenced by the inhibition zone diameter and MIC value.


Author(s):  
Alireza Daneshkazemi ◽  
Hengameh Zandi ◽  
Abdolrahim Davari ◽  
Mahmood Vakili ◽  
Majid Emtiazi ◽  
...  

Objectives: The objective of this study was to evaluate the antimicrobial activity of the essential oil obtained from oleo-gum-resin and seeds of Ferula assa-foetida. Materials and Methods: Ferula assa-foetida plants were collected from Tabas, Yazd Province, Iran, during summer 2017. Then, essential oils were obtained from its seeds and oleo-gum-resin using hydrodistillation. Gas chromatography-mass spectrometry (GC-MS) test was performed to determine the contents of the essential oils. Four different concentrations of each oil were prepared (2.5, 5, 10, and 20 μg/ml), and the antimicrobial activity of each dose against four oral bacteria (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, Streptococcus salivarius, and Lactobacillus rhamnosus) was evaluated using the disk diffusion method. The data were analyzed using analysis of variance (ANOVA) and Kruskal-Wallis test in SPSS 17 software. Results: The GC-MS findings exhibited that the main compounds found in essential oils yielded from the seeds and oleo gum resin were (Z) -1-propenyl sec-butyl disulfide and (E) -1-propenyl sec-butyl disulfide. Ferula assa-foetida plant showed a significant antimicrobial effect (P<0.05). The essential oil from Ferula assa-foetida oleo-gum-resin had significantly stronger antibacterial properties compared to the essential oil from Ferula assa-foetida seeds (P<0.001). Both essential oils showed antibacterial properties similar to that of Chlorhexidine. The growth inhibition zone was significantly dependent on the essential oil concentration for all bacteria (P<0.05). Conclusion: Our study revealed that essential oils from seeds and oleo-gum-resin of Ferula assa-foetida have antimicrobial properties. More laboratory studies are required to reach a definitive conclusion.


2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric


2021 ◽  
Vol 12 (6) ◽  
pp. 8003-8034

Compounds useful for drugs, cosmetics, and food have been obtained directly or indirectly from living organisms over the years. However, there has been a renewed interest in getting useful compounds from living organisms, especially plants. Essential oils, interchangeably called volatile oils, are bioactive compounds found in minute quantities in some plants. Essential or volatile oils have been known for years to find usefulness in foods, drugs (antimicrobial, antifungal), and cosmetics. This review attempts to summarize information on the essential oil from Ficus species concerning their morphology, pharmacology, bioactivity, and application. This was achieved by gathering information on essential oils from different Ficus species. Essential oils from Ficus species are a good source of bioactive compounds for use in drug, food, and cosmetic industries. It is worthy to note that Nigerian Figs were characterized by the high presence of phytol and 6,10,14-trimethyl-2-pentadecanone, and these compounds are, therefore, seen as markers. Furthermore, this review presents numerous insights on how to best harness the different potentials of the essential oils and possibilities to be examined.


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