scholarly journals The effect of 2,4-dichlorophenoxyacetic acid, benzyl amino purin and cupric sulphate on in vitro propagation system from shoot apices of shoot tiller of hybrid Napier grass (Pennisetum purpureum Schum)

2022 ◽  
Vol 951 (1) ◽  
pp. 012088
Author(s):  
N Umami ◽  
E R V Rahayu ◽  
B Suhartanto ◽  
N Suseno
Keyword(s):  
Multiple Shoot ◽  
Napier Grass ◽  
Plant Production ◽  
Pennisetum Purpureum ◽  
Induction Medium ◽  
Regeneration System ◽  
Shoot Apices ◽  
Regeneration Stage ◽  
Murashige Skoog

Abstract An efficient micropropagation method of hybrid Napier grass (Pennisetum purpureumn Schum) for in vitro plant production and material breeding was established from multiple-shoot clumps (MCS) regeneration system. This system was important for forage breeding system. Shoot apices from shoot-tillers produced MSC on Murashige-Skoog (MS) induction medium containing several combinations of BAP and 2,4-D in induction stage. The addition of 5 μM (v/v) and 50 μM (v/v) CuSO4 were added in best medium for inoculation to proliferate the clump in proliferation/multiplication stage. Plant regeneration was achieved by culturing on solid MS with several combination of medium containing NAA and BAP in regeneration stage. The best results for induction were Murashige-Skoog (MS) induction medium containing 2 mgL−1 BAP and 0.1 mgL−1 2,4-D. The proliferation stage on MS medium containing 5 μM CuSÜ4 effective for proliferation (50% multiple shoot formation). The regeneration stage using 0.1 mgL−1 NAA and 2.0 mgL−1 BAP (51.6% number of shoot can regenerate). All plantlets were successfully grown up in an acclimatization stage. Based on the results, the hybrid Napier grass regeneration via MSC was a stable tissue culture system (no albino plats), which could be applied either for further genetic transformation assay or for alternative supply of nursery plant in the future.

scholarly journals Establishment of a Rapid Plant Regeneration System in Physalis angulata L. through Axillary Meristems

2015 ◽  
Vol 7 (4) ◽  
pp. 471-474
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Shoot Culture ◽  
Shoot Multiplication ◽  
Multiple Shoot ◽  
Herbaceous Plant ◽  
Axillary Meristem ◽  
Regeneration System ◽  
Axillary Meristems ◽  
Meristem Explants ◽  
Physalis Angulata

An optimal plant propagation method of Physalis angulata L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum in vitro response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA3) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective in vitro shoot elongation and rooting was achieved on 1.0mg/L(GA3) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent.  This protocol can be used for commercial propagation and for future genetic improvement studies.

scholarly journals Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture

2011 ◽  
Vol 38 (No. 1) ◽  
pp. 27-34 ◽  
Author(s):  
A.A. Malik ◽  
Cui Li ◽  
Zhang Shuxia ◽  
Chen Jin-feng
Keyword(s):  
Ssr Markers ◽  
Doubled Haploids ◽  
Parent Material ◽  
Plant Production ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Heterozygous Parent ◽  
Pre Treatment ◽  
Homozygous Diploid

The effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries were investigated. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. The temperature pre-treatment (4°C) for 4 days increased embryo formation frequency (63.3%) significantly. Addition of thidiazuron (0.04 and 0.02 mg/l) in the induction medium significantly increased the number of responding ovaries (46.6%, 65.83%), respectively. The maximum number of plantlet regeneration (22.5%) was achieved by culturing the ovary derived embryos on Murashigue and Skoog medium (MS medium) supplement with 0.6 mg/l 6-benzylaminopurine. Spontaneous doubled haploids originated directly through embryogenesis were subjected to genetic analysis using SSR molecular marker with 23 primers pair for homozygosity. SSR markers with microsatellite CMGA172, confirmed that the alleles in the parental material were also present in the gynogenic plantlets, but amplified only two alleles as compared to four alleles of the heterozygous parent material at same locus. Therefore these regenerated plantlets were consider homozygous and produced through a process of gametophytic embryogenesis.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

Cell-Wall Constituents and In Vitro Digestibility of Napier Grass (Pennisetum Purpureum)

1973 ◽  
Vol 37 (5) ◽  
pp. 1255-1261 ◽  
Author(s):  
William L. Johnson ◽  
Javier Guerrero ◽  
Danilo Pezo
Keyword(s):  
Cell Wall ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility

scholarly journals 117 An In Vitro Regeneration System for Basil (Ocimum basilicum L.)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 461E-461
Author(s):  
Winthrop B. Phippen ◽  
James E. Simon
Keyword(s):  
In Vitro Regeneration ◽  
Morphological Characteristics ◽  
Leaf Explants ◽  
Basal Medium ◽  
Induction Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Transformation Protocol ◽  
Regeneration Procedure

A plant regeneration protocol was successfully developed for basil (O. basilicum L.). Explants from 1-month-old seedlings yielded the highest frequency of regeneration of shoots (37%) with an average number of 3.6 shoots per explant. Calli and shoot induction were initiated on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ) (4 mg/L) for ≈30 days. Shoot induction and development was achieved by refreshing the induction medium once after 14 days. The most morphogenetically responsive explants were basal leaf explants from the first fully expanded true leafs of greenhouse-grown basil seedlings. Developing shoots were then rooted on MS media in the dark without TDZ. Within 20 days, rooted plantlets were transferred and acclimatized under greenhouse conditions where they developed normal morphological characteristics. This is the first report of a successful in vitro regeneration system for basil through primary callus. The establishment of a reliable regeneration procedure is critical when developing a transformation protocol for enhancing the production of basil for insect and disease resistance and improved essential oil constituents.

scholarly journals An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

2015 ◽  
Vol 15 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Jyothi Abraham ◽  
T. Dennis Thomas
Keyword(s):  
Age Groups ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Multiple Shoot Induction ◽  
Elephantopus Scaber ◽  
Cotyledonary Node Explants

An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoots per explant. The highest frequency of rooting (100%) and mean number of roots (3.3 per shoot) were observed when the shoots were transferred to MS medium supplemented with 1.0 mg L-1 IBA. The plantlets raised in vitro were acclimatized and transferred to soil with a 92% success rate. The protocol described here may be utilized for multiplication and conservation of elite clones of E. scaber.

scholarly journals In vitro plant regeneration of Paulownia tomentosa (Thunb.) Steud. from shoot tip and leaf segment

2018 ◽  
Vol 44 (3) ◽  
pp. 459-463
Author(s):  
PK Roy
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Leaf Segment ◽  
Induction Medium ◽  
Paulownia Tomentosa ◽  
Shoot Induction Medium ◽  
Number Of Shoots

An efficient protocol was developed for in vitro mass propagation of Paulownia tomentosa (Thunb.) Steud. using shoot tip and leaf segment explants from field grown plant. Different concentrations and combinations of BAP, Kn, zeatin and NAA were used for multiple shoot regeneration. Among two types of explants, leaf segment produced the highest number of shoots per explant (12 ± 0.4) when they were cultured on MS supplemented with 3.0 mg/l Kn and 0.5 mg/l NAA. Addition of 10% CW to above mentioned medium increased the number of shoots (18) per culture. Shoot tip explants also produced multiple shoots in the same medium, but their performance was not good as leaf segment explants. For shoot elongation, 100 mg/l urea was more effective when added with best shoot induction medium. Shoots rooted well in halfstrength MS supplemented with 2.0 mg/l NAA, within 12 - 15 days. Regenerated plantlets were successfully acclimatized and established in poly bag containing a mixture of soil and compost in 2:1 ratio. About 90% plantlets survived under open field conditions.

scholarly journals An efficient protocol for rapid plant regeneration from deembryonated cotyledons of black gram [Vigna mungo (L.) Hepper]

2019 ◽  
Author(s):  
R. Anandan ◽  
T. Deenathayalan ◽  
R. Bhuvaneshwari ◽  
M. Merlin Monisha ◽  
M. Prakash
Keyword(s):  
Shoot Elongation ◽  
Multiple Shoot ◽  
Vigna Mungo ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Black Gram ◽  
Regeneration System ◽  
Direct Shoot Regeneration ◽  
Multiple Shoot Induction

Here an efficient protocol for micropropagation of black gram [Vigna mungo (L.) Hepper] cv. VBN 3 is reported. The deembryonated cotyledonary explants were cultured on MS medium containing different concentrations of plant growth regulators. The maximum frequency (72%) of direct shoot regeneration (devoid of callus phase), multiple shoot induction and shoot elongation was achieved from culturing the explants on MS medium containing 3.0 mg/l of 6-benzylaminopurine (BAP). Up to 65% of the regenerated shoots were rooted on MS medium containing 0.25 mg/l of á-naphthalene acetic acid (NAA) within 3 weeks after subculturing. The in vitro-raised plantlets were successfully hardened first under culture room conditions with 62% survival rate and then in greenhouse. The identified regeneration system could be efficiently used in various in vitro manipulation studies in black gram as well.

scholarly journals Biomass yield and fodder quality of Napier grass (Pennisetum purpureum) as affected by Pigeon pea (Cajanus cajan) intercrop and planting distance

2019 ◽  
Vol 54 (2) ◽  
pp. 36-44
Author(s):  
E. A. Tenakwa ◽  
S. Cudjoe ◽  
T. Ansah
Keyword(s):  
Cajanus Cajan ◽  
Biomass Yield ◽  
Pigeon Pea ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Fodder Quality ◽  
Significant Difference ◽  
Planting Distance

The study sought to investigate the effect of two planting distances and Pigeon Pea (Cajanus cajan) intercrop on the biomass yield, chemical composition and in vitro organic matter digestibility (IVOMD) of Napier grass (Pennisetum purpureum). The treatments were laid in a 2x2 factorial RCBD. The factors were two planting distances (0.5x0.5 and 0.5x1m) and two levels of intercrop (Pigeon Pea intercrop and sole Napier grass). At 90-days, the grasses were harvested and biomass yield measured. The fodder was separated into leaves and stems for chemical and digestibility analysis in 2x2x2 factorial in CRD. The interaction at all levels was not significant for all the parameters. There was a significant main effect of planting distance, intercrop and botanical fraction on biomass yield, plant height, tiller number and fodder quality. Whilst biomass yield was higher (P=0.02) in the 0.5x0.5m, no significant difference was recorded in the effect of the intercrop. Whilst intercrop affected (P<0.05) IVOMD and ME, botanical fraction significantly affected DM, CP and ME. The leaf fraction had more (P<0.05) DM, CP and ME than the stem fraction. The study revealed that close planting distance enhanced biomass yield whilst Pigeon Pea intercrop and botanical fraction affected fodder quality of Napier grass.

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