scholarly journals Preliminary phytochemical screening and determination of total polyphenols and flavonoids content in the leaves of Houttuynia cordata Thunb

2020 ◽  
Vol 736 ◽  
pp. 062013
Author(s):  
V T Nguyen ◽  
V M Le ◽  
T S Vo ◽  
L M Bui ◽  
H L T Anh ◽  
...  
Keyword(s):  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Houttuynia Cordata ◽  
Houttuynia Cordata Thunb

Microscopic features and phytochemistry of two Congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, and Aframomum angustifolium (Sonn.) K. Schum. (Zingiberaceae).

2021 ◽  
Vol 1 (2) ◽  
pp. 043-052
Author(s):  
Clément Liyongo Inkoto ◽  
Koto-Te-Nyiwa Ngbolua ◽  
Adrien Tuwisana Masunda ◽  
Carlos Nkongolo Kabengele ◽  
Jeff Bekomo Iteku ◽  
...  
Keyword(s):  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Traditional Healers ◽  
Phytochemical Screening ◽  
Calcium Oxalate Crystals ◽  
Total Polyphenols ◽  
Microscopic Features ◽  
Chemical Groups ◽  
First Time

The leaves and seeds of Aframomum alboviolaceum and Aframomum angustifolium are specifically used by traditional healers in the Democratic Republic of Congo (DRC) for the treatment of several pathologies. The aim of present study was to determine the microscopic characteristics and phytochemical composition of these species of the genus Aframomum. The microscopic study of these plants revealed the presence of punctate vessels, fiber cluster with calcium oxalate crystals, isolated unicellular hair, fibers, starch grains, spiral vessel fragments, fiber fragments, and indistinct parenchyma fragments. Phytochemical screening revealed several chemical groups such as phenolic acids, flavonoids, coumarins, alkaloids, terpenes, iridoids, saponins etc. The determination of total polyphenols, flavonoids and tannins gave contents ranging from 14.95 ± 0.45 and 63.98 ± 2.04 mgGAE/g, 0.16 ± 0.01 and 10.68 ± 0.32 mgQE/g and between 1.28 ± 0.03 and 28.51 ± 0.56 mg CAE/g respectively. In general, the leaves are richer in secondary metabolites, polyphenols, flavonoids and tannin than the seeds. Both plants also contain iron, magnesium, calcium and sodium. To our knowledge, this is the first time that histological elements have been identified in the leaves and seeds of A. angustifolium.

scholarly journals Gas Chromatographic/Mass Spectrometric Analysis of the Essential Oil of Houttuynia cordata Thunb by Using On-Column Methylation with Tetramethylammonium Acetate

2007 ◽  
Vol 90 (1) ◽  
pp. 60-67 ◽  
Author(s):  
Muhammad Ishtiaq Ch ◽  
Yang F Wen ◽  
YiYu Cheng
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Oil ◽  
Capric Acid ◽  
Inlet Temperature ◽  
Good Efficiency ◽  
Houttuynia Cordata ◽  
Acid Methyl ◽  
Houttuynia Cordata Thunb

Abstract This paper describes a simple and novel on-column derivatization procedure used with gas chromatography/mass spectrometry (GC/MS) for the analysis of essential oil of Houttuynia cordata Thunb (HCT), a traditional Chinese medicine. In the procedure, the essential oil was obtained by hydrodistillation, and the fatty acid components were derivatized with tetramethylammonium acetate (TMAA) at 250°C and identified by GC/MS. Methylation improved the determination of both the fatty acids and the other components in the essential oil of HCT. To obtain optimum methylation conditions, several important factors were investigated with pentadecane as the internal standard and a GC inlet temperature of 250°C. Tetramethylammonium hydroxide (TMAH) and TMAA were compared as the derivatization agent, and a 2:1 ratio of TMAA to capric acid was evaluated. Fatty acid methyl esters produced good chromatographic peak shapes and did not interfere with the determination of dodecanal and caryophyllene. TMAA is a neutral methylation reagent, and it yielded no side reactions during derivatization. It was found that the fatty acid content of the essential oil was about 81%; among the methylated fatty acids found were capric acid, methyl (43.66%), methyl laurate (16.15%), methyl hexadecanoate (9.27%), undecanoic acid, methyl (5.62%), methyl oleate (1.98%), and methyl linoleate (1.40%). Other major constituents were (-)-β-pinene (1.02%), β-myrcene (1.62%), 1-terpinen-4-ol (1.59%), decanal (1.49%), and 2-undecanone (1.47%). The results obtained demonstrated good efficiency for the procedure. Pure chromatograms allowed quantitation, which was obtained by total volume integration. The on-column derivatization procedure was simple to perform, and it improved the sensitivity, the peak resolution, and the selectivity of the GC/MS determination.

scholarly journals Phytochemical screening, determination of total polyphenols and flavonoids, and evaluation of the antibacterial activity of leaves of Turraea heterophylla Smith (Meliaceae)

2021 ◽  
Vol 10 (5) ◽  
pp. 16-21
Author(s):  
Kouadio Kouassi Blaise ◽  
Koffi Muriel Affouet ◽  
Oussou Kouamé Raphael ◽  
Ahoua Angora Rémi Constant ◽  
Kablan Ahmont Landry Claude ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phytochemical Screening ◽  
Total Polyphenols

scholarly journals The Qualitative And Quantitative Determination Of The Phenolic Compounds In Polygonum Convolvulus L. Species, Polygonaceae Family

2013 ◽  
Vol 59 (3) ◽  
pp. 162-164 ◽  
Author(s):  
O T Olaru ◽  
Adriana Iuliana Anghel ◽  
Viorica Istudor ◽  
Iulia Ioana Olaru
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Polyphenolic Compounds ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Qualitative And Quantitative ◽  
Polygonum Convolvulus ◽  
Subalpine Zone ◽  
Layer Chromatography

Abstract Introduction: Polygonum convolvulus L. (black bindweed), syn. Fallopia convolvulus (L.) Á. Löve, Polygonaceae family is a plant from the spontaneous flora, spread from the plain zone up to the subalpine zone. The objectives of our researches are the qualitative and quantitative determination of polyphenolic compounds from Polygoni convolvuli herba and the choice of the adequate solvent for obtaining an active pharmacological extract. Method: The qualitative exam consisted of phytochemical screening and thin layer chromatography. The quantitative determination of the total polyphenols was made through the Folin-Ciocâlteu method. Results: The flavonoids, the anthocyanins, the tannins and the phenol carboxylic acids (phytochemical screening) were emphasized and the following compounds were identified: rutin, hyperoside, isoquercitroside, quercetin, myricetin, kaempferol and caffeic acid. Conclusions: In order to establish the technological lab process for obtaining an active pharmacological extract standardized in total polyphenols the adequate solvent is ethanol 50% (v/v).

scholarly journals KARAKTERISTIK DAN SKRINING FITOKIMIA EKSTRAK ETANOL 70% BATANG KEPUH (Sterculia foetida L.)

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 22
Author(s):  
N. P. S. E. Cahyani ◽  
J. Susiarni ◽  
K. C.S. Dewi ◽  
N. L.P. Melyandari ◽  
K. W.A. Putra ◽  
...  
Keyword(s):  
Moisture Content ◽  
Water Content ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Residual Solvent ◽  
Solvent Content ◽  
Residual Content ◽  
Ethanol Level ◽  
Anti Inflammatory

Kepuh (Sterculia foetida L) is a type of kapok plant that has been scientifically proven to have activity as an anti-inflammatory and analgesic. 70% ethanol extract of stem stem is obtained by maceration using 70% ethanol. Examination of the characteristics of 70% ethanol extract of kepuh stem included organoleptic, of moisture content and determination of residual solvent content. Phytochemical screening of 70% ethanol extract of kepuh bark includes: alkaloid, flavonoid, saponin, tannin , polyphenol, and examination of glycosides. The results of the examination of the characteristics of 70% ethanol extract of kepuh stem obtained water content of 8.66 ± 0.748%, the residual content of the solvent had a 0 (zero) ethanol level. The results of phytochemical screening showed 70% ethanol extract of stem stem containing steroid compounds, triterpenoids, flavonoids, saponins, tannins and polyphenols. The identification using UV-Vis spectrophotometry produced ? 212, the absorbance was 1.8601 and ? 284, the absorbance was 0.42186.

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