scholarly journals Phytochemical screening, determination of total polyphenols and flavonoids, and evaluation of the antibacterial activity of leaves of Turraea heterophylla Smith (Meliaceae)

2021 ◽  
Vol 10 (5) ◽  
pp. 16-21
Author(s):  
Kouadio Kouassi Blaise ◽  
Koffi Muriel Affouet ◽  
Oussou Kouamé Raphael ◽  
Ahoua Angora Rémi Constant ◽  
Kablan Ahmont Landry Claude ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phytochemical Screening ◽  
Total Polyphenols

Microscopic features and phytochemistry of two Congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, and Aframomum angustifolium (Sonn.) K. Schum. (Zingiberaceae).

2021 ◽  
Vol 1 (2) ◽  
pp. 043-052
Author(s):  
Clément Liyongo Inkoto ◽  
Koto-Te-Nyiwa Ngbolua ◽  
Adrien Tuwisana Masunda ◽  
Carlos Nkongolo Kabengele ◽  
Jeff Bekomo Iteku ◽  
...  
Keyword(s):  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Traditional Healers ◽  
Phytochemical Screening ◽  
Calcium Oxalate Crystals ◽  
Total Polyphenols ◽  
Microscopic Features ◽  
Chemical Groups ◽  
First Time

The leaves and seeds of Aframomum alboviolaceum and Aframomum angustifolium are specifically used by traditional healers in the Democratic Republic of Congo (DRC) for the treatment of several pathologies. The aim of present study was to determine the microscopic characteristics and phytochemical composition of these species of the genus Aframomum. The microscopic study of these plants revealed the presence of punctate vessels, fiber cluster with calcium oxalate crystals, isolated unicellular hair, fibers, starch grains, spiral vessel fragments, fiber fragments, and indistinct parenchyma fragments. Phytochemical screening revealed several chemical groups such as phenolic acids, flavonoids, coumarins, alkaloids, terpenes, iridoids, saponins etc. The determination of total polyphenols, flavonoids and tannins gave contents ranging from 14.95 ± 0.45 and 63.98 ± 2.04 mgGAE/g, 0.16 ± 0.01 and 10.68 ± 0.32 mgQE/g and between 1.28 ± 0.03 and 28.51 ± 0.56 mg CAE/g respectively. In general, the leaves are richer in secondary metabolites, polyphenols, flavonoids and tannin than the seeds. Both plants also contain iron, magnesium, calcium and sodium. To our knowledge, this is the first time that histological elements have been identified in the leaves and seeds of A. angustifolium.

scholarly journals Penapisan Fitokimia, Kadar Kurkuminoid dan Aktivitas Antibakteri Temu Hitam (Curcuma aeruginosa (Christm) Roscoe.), Temu Putih (Curcuma zedoaria Roxb.) dan Temulawak (Curcuma xanthorrhiza Roxb.)

2021 ◽  
Vol 4 (1) ◽  
pp. 57
Author(s):  
Lia Marliani ◽  
Ika Kurnia Sukmawati ◽  
Dadang Juanda ◽  
Elmadhita Anjani ◽  
Ira Anggraeni
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Phytochemical Screening ◽  
Curcuma Zedoaria ◽  
Propionibacterium Acne ◽  
Microdilution Method ◽  
Turmeric Extract ◽  
Curcuma Xanthorrhiza

The content of secondary metabolites in the rhizome of the Curcuma genus such as Black turmeric (Curcuma aeruginosa Roxb.), White turmeric (Curcuma zedoaria (Christm.) Roscoe) and Java turmeric (Curcuma xanthorrhiza Roxb.) play a role in various pharmacological activities. One of them is the content of the curcuminoid compounds which have been proved to have antibacterial activity. This study aims to screen the content of secondary metabolite compounds, determine curcuminoid content and verify the antibacterial activity of the extracts of Black turmeric (Curcuma aeruginosa Roxb.), White turmeric (Curcuma zedoaria (Christm.) Roscoe) and Java turmeric (Curcuma xanthorrhiza Roxb.). Extraction was carried out by the maceration method using 95% ethanol as solvent. Phytochemical screening was tested for the content of alkaloid, polyphenols, flavonoids, quinones, tannins, saponins, and steroids/ triterpenoids compounds. Determination of curcuminoid content by UV-Vis Spectrophotometry method. The antibacterial activity test was carried out by the microdilution method against Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne bacteria. The results of phytochemical screening showed that the three extracts contained polyphenols and flavonoids. Quinone compounds are only contained in the extract of Black turmeric and Java turmeric. Saponin compounds were only detected in Black turmeric and White turmeric  extracts. Meanwhile, steroid/ triterpenoid compounds were detected in the extract of White turmeric and Java turmeric. The results of curcuminoid content determination on the three extracts showed that the Java turmeric extract had the highest content of curcuminoids (16.07 ± 0.023 mg CE/g extract). The results of the antibacterial test showed the strongest activity of the three test samples shown by Java turmeric extract with a minimum inhibitory concentration (MIC) value of 64 mg/mL against Staphylococcus aureus bacteria; 256 mg/mL against Staphylococcus epidermidis bacteria; and 32 mg/mL against the Propionibacterium acne bacteria. These results indicate that Java turmeric extract is more active against Propionibacterium acne bacteria.

scholarly journals Penetapan Kadar Flavonoid Dan Uji Aktivitas Antibakteri Ekstrak Sereh (Cymbopogon nardus) Dan Temu Kunci (Boesnbergia pandurata Roxb) Terhadap Bakteri Streptococcus Mutans

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Anita Kumala Hati ◽  
Niken Dyahariesti ◽  
Richa Yuswantina
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Average Diameter ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Color Test ◽  
Disk Diffusion Method ◽  
Cymbopogon Nardus

Indonesia is rich in medicinal plants, including those that have the ability to inhibit bacterial growth, lemongrass (Cymbopogon nardus) and Fingerroots (Boesenbergia pandurata). Streptococcus mutans is a bacterium that causes dental caries. This Experiment want to determine the content of chemical compounds and the antibacterial activity of Lemongrass extract (Cymbopogon nardus) and Fingerroots (Boesenbergia pandurata) against Streptococcus mutans. The study was conducted experimentally with samples of lemongrass and Fingerroots. Extraction using maceration method using 70% Ethanol solvent. Phytochemical screening test with color test and determination of total flavonoid levels using a spectrophotometer. Antibacterial activity test uses the disk diffusion method against Streptococcus mutans. Phytochemical screening results of lemongrass extract and fingerroots with color test qualitative test showed positive containing flavonoids, saponins and tannins. In the test determination of total flavonoid levels, showed an average total flavonoid levels in lemongrass extracts of 48.61 mgQE / g and in Fingerroots extracts of 24.71 mgQE / g. The results of the antibacterial test against Streptococcus mutans obtained the largest average diameter of inhibitory zone in the extract of Fingerroots 5% w / v (11.167 mm), then the concentration of 5% w / v combination of lemongrass: Fingerroots 1: 2 (10.83 mm ), 2: 1 combination (10,067 mm). lemongrass (9.33 mm), 1: 1 combination (9,133 mm), Based on the Post Hoc test results that were comparable to positive controls were 5% fingerrots, combination of lemongrass: 1: 2 Fingerroots, and 2: 1 combination. The average total flavonoid level in lemongrass extract is more than the average total flavonoid content in Fingerroots. But in the same concentration 5% Fingerroots extract provides the greatest antibacterial inhibitory activities.

scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2017 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Physalis Angulata

<p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (Physalis angulata L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of P.angulata extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from P.angulata extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes Staphylococcus aureus and Klebsiella pneumoniae was done by using checkerboard method. The results of phytochemical screening showed that simplicia and P.angulata extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that P.angulata extract had antibacterial activity against S.aureus and K.pneumoniae with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of S.aureus is demonstrated by the combination of P.angulata herb extract with tetracycline. The additive/indifferent interaction of S.aureus is demonstrated by the combination of P.angulata extracts with ampicillin, and against K.pneumoniae shown by the combination of P.angulata extract of cecendet with ampicillin or tetracycline. Ethanol extract of P.angulata has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of S.aureus and K.pneumoniae bacteria.</em></p><p align="center"><strong><em> </em></strong></p><p><strong><em>Keywords</em></strong><em>:  Cecendet, Physalis angulata L., MIC, antibiotic combination</em></p>

scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2018 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Physalis Angulata

<p align="center"><strong>Abstrak</strong></p><p> </p><p>Penyakit infeksi merupakan salah satu masalah kesehatan terbesar tidak saja di Indonesia, namun di seluruh dunia. Bakteri merupakan agen penting dalam menyebabkan penyakit infeksi. Seringkali masyarakat Indonesia menggunakan antibiotik bersamaan dengan penggunaan obat herbal. Cecendet (<em>Physalis angulata</em> L.) merupakan salah satu tanaman tradisional yang memiliki aktivitas  antibakteri dan banyak digunakan oleh masyakat Indonesia secara turun menurun. Penelitian ini  bertujuan untuk menguji aktivitas antibakteri herba cecendet dan pengaruhnya jika digunakan kombinasi dengan antibiotik. Pembuatan ekstrak herba cecendet dilakukan menggunakan metode refluks dengan pelarut etanol 50%. Terhadap simplisia dan ekstrak yang diperoleh, dilakukan pemeriksaan karakteristik dan penapisan fitokimia. Penentuan aktivitas antibakteri dari ekstrak herba cecendet dilakukan dengan metode uji mikrodilusi dengan menilai konsentrasi hambat minimum (KHM). Penentuan efektivitas kombinasi  ekstrak herba cecendet dengan antibiotik (ampisilin/tetrasiklin) terhadap <em>Staphylococcus aureus dan Klebsiella pneumoniae</em> dilakukan dengan menggunakan metode papan catur (<em>Checkerboard</em>).  Hasil penapisan fitokimia menunjukkan bahwa simplisia dan ekstrak etanol herba cecendet mengandung alkaloid, flavonoid, saponin, polifenol, monoterpenoid, seskuiterpen, steroid dan triterpenoid. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak etanol herba cecendet mempunyai aktivitas antibakteri terhadap <em>S.aureus dan K.pneumoniae</em> dengan konsentrasi hambat minimum berturut-turut adalah 128 µg/mL, 256 µg/mL. Interaksi yang sinergis terhadap <em>S.aureus</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan tetrasiklin. Interaksi yang aditif/indifferent terhadap <em>S.aureus</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan ampisilin, dan terhadap <em>K.pneumoniae</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan ampisilin atau tetrasiklin. Ekstrak etanol herba cecendet memiliki aktivitas antibakteri dan dan kombinasi dengan antibiotik (ampisilin/tetrasiklin) dapat memberikan efek sinergis atau aditif bakteri <em>S.aureus</em> dan <em>K.pneumoniae.</em></p><p> </p><p><strong>Kata kunci:  </strong>Cecendet, <em>Physalis angulata</em> L., konsentasi hambat minimum, antibakteri, kombinasi antibiotik</p><p> </p><p> </p><p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (<em>Physalis angulata</em> L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of <em>P.angulata</em> extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from <em>P.angulata</em> extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes <em>Staphylococcus aureus</em> and <em>Klebsiella pneumoniae</em> was done by using checkerboard method. The results of phytochemical screening showed that simplicia and <em>P.angulata</em> extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that <em>P.angulata</em> extract had antibacterial activity against <em>S.aureus</em> and <em>K.pneumoniae</em> with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of <em>S.aureus</em> is demonstrated by the combination of <em>P.angulata</em> herb extract with tetracycline. The additive/indifferent interaction of <em>S.aureus</em> is demonstrated by the combination of <em>P.angulata</em> extracts with ampicillin, and against <em>K.pneumoniae</em> shown by the combination of <em>P.angulata</em> extract of cecendet with ampicillin or tetracycline. Ethanol extract of <em>P.angulata</em> has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of<em> S.aureus</em> and <em>K.pneumoniae</em> bacteria.</p><p align="center"><strong><em> </em></strong></p><p><strong>Keywords</strong>: Cecendet, <em>Physalis angulata</em> L., MIC, antibiotic combination</p>

scholarly journals Biochemical Studies on Bio Extracts as Antioxidant and Antibacterial Activity

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Farid, M, Hanaa, F. M. Ali, Gehan, F. A. Massoud, Sherein S.
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Tilia Cordata ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Antioxidants Activity ◽  
Biochemical Studies

Biochemical Evaluated of some bio extracts as follows: Chemical composition and Phytochemical screening were determined in Tilia Cordata and Vitex Agnus leaves. The total polyphenols and flavonoids contents of Tilia Cordata leaves have the highest of total polyphenols and flavonoids contents, which were 126.00mgGAE/g and 15.88mgQE/g, followed by Vitex Agnus leaves, which were 119.77mgGAE/g and 13.41mgQE/g, respectively. Methanolic and aqueous extracts of plants leaves were antioxidant activity by used (FRAP, LPO, DPPH and ABTS) The methanolic extract of Tilia Cordata and Vitex Agnus  leaves, have the highest of reducing power which were 1.649 and 1.018 at the concentrations of 80mg/ml, respectively. Also, by used (LPO, DPPH and ABTS), were the highest antioxidants activity for methanolic extract respectively. Moreover, the methanolic extract of Tilia Cordata leaves produced the highest growth inhibition (18 and 17mm) for against Escherichia coli and Bacillus subtillis at 4mg/ml, respectively. While, The methanolic extract of Vitex Agnus produced the medium percentage of growth inhibition (10.5, 9.75 and 10mm) for against Escherichia coli, St.coccus aureus and Bacillus subtillis at 4mg/ml, respectively. And compared with antibiotic. This study concluded that Tilia Cordata and Vitex Agnus  leaves extract has an antioxidant and antibacterial activity.

scholarly journals The Qualitative And Quantitative Determination Of The Phenolic Compounds In Polygonum Convolvulus L. Species, Polygonaceae Family

2013 ◽  
Vol 59 (3) ◽  
pp. 162-164 ◽  
Author(s):  
O T Olaru ◽  
Adriana Iuliana Anghel ◽  
Viorica Istudor ◽  
Iulia Ioana Olaru
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Polyphenolic Compounds ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Qualitative And Quantitative ◽  
Polygonum Convolvulus ◽  
Subalpine Zone ◽  
Layer Chromatography

Abstract Introduction: Polygonum convolvulus L. (black bindweed), syn. Fallopia convolvulus (L.) Á. Löve, Polygonaceae family is a plant from the spontaneous flora, spread from the plain zone up to the subalpine zone. The objectives of our researches are the qualitative and quantitative determination of polyphenolic compounds from Polygoni convolvuli herba and the choice of the adequate solvent for obtaining an active pharmacological extract. Method: The qualitative exam consisted of phytochemical screening and thin layer chromatography. The quantitative determination of the total polyphenols was made through the Folin-Ciocâlteu method. Results: The flavonoids, the anthocyanins, the tannins and the phenol carboxylic acids (phytochemical screening) were emphasized and the following compounds were identified: rutin, hyperoside, isoquercitroside, quercetin, myricetin, kaempferol and caffeic acid. Conclusions: In order to establish the technological lab process for obtaining an active pharmacological extract standardized in total polyphenols the adequate solvent is ethanol 50% (v/v).

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