scholarly journals KARAKTERISTIK DAN SKRINING FITOKIMIA EKSTRAK ETANOL 70% BATANG KEPUH (Sterculia foetida L.)

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 22
Author(s):  
N. P. S. E. Cahyani ◽  
J. Susiarni ◽  
K. C.S. Dewi ◽  
N. L.P. Melyandari ◽  
K. W.A. Putra ◽  
...  
Keyword(s):  
Moisture Content ◽  
Water Content ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Residual Solvent ◽  
Solvent Content ◽  
Residual Content ◽  
Ethanol Level ◽  
Anti Inflammatory

Kepuh (Sterculia foetida L) is a type of kapok plant that has been scientifically proven to have activity as an anti-inflammatory and analgesic. 70% ethanol extract of stem stem is obtained by maceration using 70% ethanol. Examination of the characteristics of 70% ethanol extract of kepuh stem included organoleptic, of moisture content and determination of residual solvent content. Phytochemical screening of 70% ethanol extract of kepuh bark includes: alkaloid, flavonoid, saponin, tannin , polyphenol, and examination of glycosides. The results of the examination of the characteristics of 70% ethanol extract of kepuh stem obtained water content of 8.66 ± 0.748%, the residual content of the solvent had a 0 (zero) ethanol level. The results of phytochemical screening showed 70% ethanol extract of stem stem containing steroid compounds, triterpenoids, flavonoids, saponins, tannins and polyphenols. The identification using UV-Vis spectrophotometry produced ? 212, the absorbance was 1.8601 and ? 284, the absorbance was 0.42186.

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KARAMUNTING (Rhodomyrtus tomentosa.) TERHADAP PERTUMBUHAN BAKTERI Salmonella thypi

2020 ◽  
Vol 2 (2) ◽  
pp. 102-108
Author(s):  
Suci Wulandari ◽  
Chandra Pranata ◽  
Yanna Rotua Sihombing ◽  
Muhammad Hamim Nasution
Keyword(s):  
Infectious Disease ◽  
Typhoid Fever ◽  
Plant Material ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Paper Disk ◽  
Active Substances ◽  
Rhodomyrtus Tomentosa

Salmonella thypiis a bacterium that causes typhoid fever. Typhoid fever is an infectious disease that is still a health problem, especially in Indonesia. There are several studies on the types of plants that can control bacterial growth. Indonesian plants are often used as medicine, one of which is the karamunting (Rhodomyrtus tomentosa.). Traditionally a number of parasitic species have been used to prevent and treat various diseases such as cough, anticancer, diuretic, anti-inflammatory, antibacterial, wound or infection karamunting (Rhodomyrtus tomentosa.) Has active compounds such as flavonoids, alkaloids, tannins , saponins, and glycosides which have antibacterial abilities. The purpose of this study is to determine the inhibition of extract of karamunting (Rhodomyrtus tomentosa.) at concentrations of 5%, 10%, and 15%. Test samples using Salmonella thypi. This research is an experimental study with a design (posttest). The research methods included the collection of plant material, determination of plant material, making simplicia, phytochemical screening, making ethanol extract from simplicia by maceration, testing chemical compound groups, and antibacterial testing with paper disk diffusion methods. Karamunting is extracted by maceration process, using 70% ethanol solvent. The results of this study indicate that the benalu coffee leaf extract (Rhodomyrtus tomentosa.) has inhibitory capacity as an antibacterial in Salmonella thypi with a mean inhibition of 16.87 mm at a concentration of 5%, 17.24 mm at a concentration of 10%, and 18.21 mm at a concentration of 15%. The greatest inhibition is produced at a concentration of 15%, because the higher the concentration of the extract, the higher the diameter of the inhibition produced, because the more active substances contained in the extract.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT TEST OF ETHANOL EXTRACT OF TURMERIC RHIZOME (Curcuma domestica Val.)TOWARD MALE WHITE MOUSE (Rattus norvegicus)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rulia Meilina
Keyword(s):  
Ethanol Extract ◽  
White Mouse ◽  
Phytochemical Screening ◽  
Suspension Control ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Group B ◽  
Group D ◽  
Better Than ◽  
Inflammatory Effect

Anti-inflammatory effect of ethanol rhizome of turmeric was conducted toward white mouse which is induced the carrageenan solution (lambda carrageenan) 1 %, research was conducted by dividing the animals into 5 groups.Group A was given CMC 0,5%, group B was given Indometacin with dose 100 mg/kgBB, group C was given  ethanol rhizome extract of turmeric with dose 400 mg/kgBB, group D was given ethanol rhizome extract of turmeric with dose 500 mg/kgBB, group E was given ethanol rhizome extract of turmeric with dose 600 mg/kgBB. Result of phytochemical screening toward powder and ethanol extract of rhizome of turmeric shows that chemical compounds which are involved are alkaloids, flavonoids, and aetheric oil. In the other hand, result of phytochemical screening toward ethanol extract of turmeric’s rhizome are alkaloids,flavonoids,anthraquinones, glycosides. Result of anti-inflammatory test shows that there is the real difference among groups of mouse which were given CMC suspension (control), suspension of ethanol extracts of turmeric’s rhizome with dose 400 mg/kg BB, 500 mg/kg BB, 600 mg/kg BB (material test) and suspension of indomethacin with dose 100 mg/kg BB (positive comparison). Suspension of ethanol extract of turmeric’s rhizome with dose 600 mg/kg BB shows that anti-inflammatory capability is better than dose 400 mg/kg BB and 500 mg/kg BB, and suspension of ethanol extract of turmeric’s rhizome dose 600 mg/kg BB shows the anti-inflammatory effect which is not really different with suspension of indomethacin with dose 100 mg/kg BB, it is showed by  ANOVA statistic analysis among those comparisons toward indomethacin suspension as positive comparison.Keywords:Anti-inflammatory, Indomethacin, Turmeric Rhizome

scholarly journals Phytochemical screening and in vitro anti-inflammatory activity of ethanolic extract of Epidendrum coryophorum leaves.

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1387-1393
Author(s):  
Irina Francesca González Mera ◽  
Orestes Darío López Hernández ◽  
Vivian Morera Córdova
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Epidendrum coryophorum belongs to the Orchidaceae family. Traditional uses of some species for this genus include infusions of the leaves used for kidney problems, treat influenza, conjunctivitis, liver pain, relieve kidney symptoms, and hypoglycemic effect. This work's objective was to determine the phytochemical profile of the ethanolic extract of Epidendrum coryophorum leaves and to evaluate the potential anti-inflammatory activity in vitro of the extract employing the erythrocyte membrane stabilization method. The phytochemical screening carried out in this work suggested phenols, coumarins, flavonoids, tannins, steroids, and sterols in the ethanolic extract of Epidendrum coryophorum leaves. Cardiotonic glycosides and carbohydrates were also found. The ethanolic extract's UV-Vis spectrum showed absorption maxima at 268 nm and 332 nm, which could correspond to flavonoids of the flavonoid classes, 3-OH substituted flavonols, or isoflavones. The quantitative determination of total phenols of the ethanolic extract was carried out using the Folin-Ciocalteu method. The total phenolic content expressed as mg Gallic acid equivalent (G.A.E.) per gram of extract was found to be 19,96 mgGAE/g of Epidendrum coryophorum. The ethanolic extract of Epidendrum coryophorum leaves showed hemolysis inhibition values ​​of 18,19% at 1,0 mg/mL, 38,98% at 1,5 mg/mL and 40,94% at 2,5 mg/mL compared with aspirin (positive control) giving values ​​of 65,33% at 1,0 mg/mL, 72,26% at 1,5 mg/mL and 73,75% at 2,5 mg/mL. The values ​​obtained for inhibition of hemolysis with ethanolic extract, compared with the values ​​obtained with a pure anti-inflammatory, are significant and demonstrate anti-inflammatory activity in Epidendrum coryophorum. Keywords: Epidendrum coryophorum, total phenolic content, microencapsulation, anti-inflammatory activity

scholarly journals Determination of Quality Parameters and Antioxidant Activity of Cincau Hijau Leaves (Cyclea barbata L.Miers.)

2017 ◽  
Vol 15 (2) ◽  
pp. 236
Author(s):  
Erlindha Gangga ◽  
Rani Purwati ◽  
Yunahara Farida ◽  
Kartiningsih Kartiningsih
Keyword(s):  
Antioxidant Activity ◽  
Heavy Metal Contamination ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Phytochemical Screening ◽  
Water Soluble Compound ◽  
Dpph Method

Cincau Hijau leaves (Cyclea barbata L.Miers ) has been used empirically as a medicinal plant because it contains many potential compounds such as flavonoids. Cincau Hijau leaves has been known as antioxidant activity. Determination of pharmacognosy parameters and phytochemical screening of dry leaves and extracts have been carried out as well as determination of total fl avonoids content. Fresh leaves were extracts with water and Dry leaves were macerated with 96% ethanol and were then concentrated by rotavapor to obtain viscous extracts. Free radical scavenging activity of the extracts was evaluated using DPPH method. Afterwards, determination of specifi c and non-specifi c parameters were performed. Results of phytochemical screening of powder and 50% and 96% ethanol extract showed that tall the tested samples contained alkaloid, fl avonoids, saponins, tannins, steroids/ triterpenoids, coumarin. The examination of specifi c parameter showed that the extract has a thick consistency, tawny color, bitter taste, characteristic odor. In addition, water-soluble compound and 96% ethanol extract are 46.64 and 62.13% respectively whereas ethanol-soluble compounds are 39.22 and 74.72%, respectively. While the results of nonspecifi c parameters of 50% and 96 % ethanol extract displayed total ash content of 9.69 and 9.49%, respectively, acid insoluble ash content of 0.30 and 0.16%, respectively, content of water soluble ash of 9.17 and 4.30%, respectively, loss on drying of 9.35 and 8.9%, respectively, water content of 8.45 and 7.25%, respectively. Based on heavy metal contamination, Pb concentration in 50 and 96% ethanol extract are 0.0227 and 0.0333 mg/kg, respectively whereas Cd concentration are 0.1206 and 0.0022 mg/kg, respectively and total number of CFU of 4,22 x 103 and 2,30 x 103 colonies/g, respectively while molds and yeasts number of colony of 0,48 x 102 and 8,88 x 102 colonies/g, respectively. Moreover, the total flavonoid was 0,19 %. Result of DPPH inhibition test showed that IC50 96 % ethanol extract are 83,280 ppm and water extracts are 102,01 ppm

scholarly journals PENETAPAN RENDEMEN EKSTRAK DAUN JAMBU MAWAR (Syzygium jambos L. Alston) BERDASARKAN VARIASI KONSENTRASI ETANOL DENGAN METODE MASERASI

2020 ◽  
Vol 2 (3) ◽  
pp. 147-157
Author(s):  
Eka Siswanto Syamsul ◽  
Olanda Anugerah ◽  
Risa Supriningrum
Keyword(s):  
Ethanol Concentration ◽  
Ethanol Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Sample Collection ◽  
Syzygium Jambos ◽  
Significant Difference ◽  
Ethanol Extracts ◽  
Guava Leaf

Syzygium jambos is a medicinal plant. This study aims to determine the effect of variations in ethanol concentration on yield of guava leaf extract by maceration method. The research conducted was experimental research. The research phase includes sample collection, determination, manufacture of simplicia, extraction by maceration with variations in ethanol concentration, determination of yield, phytochemical screening and determination of drying losses. Phytochemical screening results showed that 50% of ethanol extracts of positive rose guava contained flavonoids, tannins, saponins and steroids / terpenoids, 70% ethanol and 90% positives contained flavonoids, tannins and steroids / terpenoids. The yield of rose guava ethanol extract was 50% (23.01% ± 0.372), 70% ethanol (21.96% ± 1.181) and 90% ethanol (16.57% ± 0.384). LSD test results showed a significant difference (p <0.05) between the 90% to 50% and 70% groups.

scholarly journals Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis

2021 ◽  
Vol 11 (4-S) ◽  
pp. 15-21
Author(s):  
Esther Del florence Ndedi Moni ◽  
Patrick Hervé Diboue Betote ◽  
Christelle Wayoue Kom ◽  
Chimène Félicite Mekoulou Benga ◽  
Armelle Deutou Tchamgoue ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Medicinal Plants ◽  
Inhibitory Action ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Resistant Strains ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The present work aimed to determine the phytochemical components and evaluate the in vitro anti-inflammatory and anti-mycobacterial effects of hydroethanolic extracts of Allium sativum L bulbs, Drypetes gossweileri S. MOORE stem-barks and Pentadiplandra brazzeana Baill roots against several resistant strains of Mycobacterium tuberculosis. The phytochemical screenings of extracts were carried out according the colorimetric and precipitation tests to reveal the presence of phytochemical compounds. The anti-inflammatory effects of extracts were evaluated using in vitro Bovine Serum Albumin denaturation and proteinase inhibitory action assays. The inhibitory parameters of hydro-ethanol extracts were evaluated by the microdilution method agaisnt Mycobacterium tuberculosis. The phytochemical screening of hydro-ethanol extracts revealed the presence of phenols, polyphenols, flavonoids, alkaloids, cathechic tannins, triterpens, steroids, anthocyanins and leucoanthocyanins. The anti-inflammatory activity of hydro-ethanol extracts of D. gossweileri, P. brazzeana and A. sativum have shown the inhibitory concentrations 50 (IC50) values ranging from 356.70, 183.30 and 226.30 mg/mL for BSA denaturation and 31.92, 33.62 and 56.93 mg/mL for proteinase inhibitory action respectively. The hydroethanolic extracts of D. gossweileri, P. brazzeana and A. sativum exhibited moderate and weak anti-mycobacterial activities with the minimum inhibitory concentrations (MICs) ranging from 312.5 to 2500 μg/mL. A. sativum hydro-ethanol extract has shown the highest anti-mycobacterial activity with MIC of 312.5 μg/mL against isoniazid resistant of M. tuberculosis and extremely resistant drug strain of M. tuberculosis. These results suggest that hydro-ethanol extracts of A. sativum, D. gossweileri and P. brazzeana are efficient against tuberculosis caused by multi-resistant Mycobacterium tuberculosis strains and are able to resorb the inflammation induced during infection. Keywords: Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening.

MICROSCOPIC AND PHYTOCHEMICAL EVALUATION OF CALOTROPIS GIGANTEA

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (06) ◽  
pp. 65-68
Author(s):  
N. K Choudhary ◽  
◽  
S. Sharma ◽  
S. Goyal ◽  
J. Dwivedi
Keyword(s):  
Ethanol Extract ◽  
Chloroform Extract ◽  
Water Soluble ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Calotropis Gigantea ◽  
Traditional System ◽  
Microscopic Evaluation

In traditional system of medicine, Calotropis gigantea (Akra) has been used for treatment of various diseases. Since, there is no proper information regarding pharmacognostic parameters of flowers of this plant, our efforts were devoted to study the microscopical characters and phytochemical study of flowers of this plant. Microscopic evaluation of gynostegium, sepal/ calyx, petal, ovary and pedicel were undertaken, determination of ash and extractive values, phytochemical screening and TLC for presence of triterpenoids. The microscopic characterization of the parts of the flower i.e. gynostegium, sepal/ calyx, petal, ovary and pedicel of Calotropis gigantea was performed. The physicochemical characterization‘s gave ash values (total ash: 18.60; acid insoluble ash: 5.46; water soluble ash; 13.50) and extraction with petroleum ether, chloroform and 90% ethanol extract gave 2.4, 3.9 and 2.1% w/w, respectively. TLC of chloroform extract showed the presence of triterpenoids. The microscopic study of the flower of Calotropis gigantea give us the foot prints for further study with the presence of triterpenoids in the flowers.

scholarly journals Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Batang Bawang Laut (Proiphys amboinensis (L.) Herb.)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 144
Author(s):  
Chendy Christy Dapas ◽  
Harry S. J. Koleangan ◽  
Meiske S. Sangi
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Steroid Saponin ◽  
Brine Shrimp Lethality ◽  
Probit Method ◽  
Dried Extract

Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman bawang laut (Proiphys amboinensis (L.) Herb.) Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia untuk senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak batang tanaman bawang laut segar dan kering dan selanjutnya dianalisis menggunakan spektrofotometer UV-VIS. Penentuan toksisitas ekstrak batang tanaman bawang laut menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh flavonoid dan  steroid positif sedangkan alkaloid, saponin triterpenoid dan tanin negatif. Analisis spektofotometer UV-VIS ekstrak etanol menunjukkan puncak serapan pada 304,40 dan  284,50 nm. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan  metode probit menggunakan perangkat lunak SPSS 20. Hasil uji toksisitas ekstrak batang tanaman bawang laut menunjukkan bahwa  ekstrak segar maupun kering bersifat sangat toksik dengan nilai LC50 kurang dari 30 ppm, yaitu: 9,978 ppm untuk batang kering dan 3,980 ppm untuk batang segar.A research has been done on the analysis of secondary metabolites and toxicity examination on the stems of the sea onion (Proiphys amboinensis (L.) Herb.). Analysis of secondary metabolites carried out with the phytochemical screening for alkaloid compounds, flavonoids, triterpenoids, steroids, saponins, and tannins in extracts of fresh and dried stems of the sea onion, then analyzed using UV-VIS spectrophotometer. The toxicity determination of stems extract of the sea onion plant was done by using Brine Shrimp Lethality Test (BSLT) with Artemia salina Leach as bioindicator. Phytochemical screening showed positive test for flavonoids and steroids while alkaloids, saponins, triterpenoids, and tannins were negative. UV-VIS  spectrophotometer analysis on ethanol extract showed peaks at 304,40 and 284,50 nm. Data of the toxicity examination was obtained from the analysis of Lethality Concentration 50 (LC50) which were performed with the probit method using SPSS 20. Results of toxicity examination of sea onion stems extract showed that fresh or dried extract are very toxic with LC50 values ​​of less than 30 ppm, namely: 9,978 ppm for fresh stems and 3,980 ppm for dried stems.

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