Mitochondrial Oxidative Phosphorylation Protein Levels in Peripheral Blood Mononuclear Cells Correlate with Levels in Subcutaneous Adipose Tissue within Samples Differing by HIV and Lipoatrophy Status

Regular physical activity prescription is a key point for healthy aging and chronic disease management and prevention. Our aim was to evaluate the antioxidant defense system and the mitochondrial status in peripheral blood mononuclear cells (PBMCs) and the level of oxidative damage in plasma in active, intermediate and inactive elderly. In total, 127 healthy men and women >55 years old participated in the study and were classified according on their level of declared physical activity. A more active lifestyle was accompanied by lower weight, fat mass and body mass index when compared to a more sedentary life-style. Active participants exhibited lower circulating PBMCs than inactive peers. Participants who reported higher levels of exercise had increased antioxidant protein levels when compared to more sedentary partakers. Carbonylated protein levels exhibited similar behavior, accompanied by a significant raise in expression of cytochrome c oxidase subunit IV in PBMCs. No significant changes were found in the activities of antioxidant enzymes and in the expression of structural (MitND5) and mitochondrial dynamic-related (PGC1α and Mitofusins1/2.) proteins. Active lifestyle and daily activities exert beneficial effects on body composition and it enhances the antioxidant defenses and oxidative metabolism capabilities in PBMCs from healthy elderly.

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Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells

Scientific Reports ◽

10.1038/s41598-019-56354-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Bàrbara Reynés ◽

Evert M. van Schothorst ◽

Jaap Keijer ◽

Andreu Palou ◽

Paula Oliver

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Peripheral Blood Mononuclear Cells ◽

Cold Exposure ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Cell Cycle Gene ◽

Peripheral Blood Mononuclear ◽

Brown Adipose ◽

Blood Mononuclear Cells

AbstractAnimal studies, mostly performed in rodents, show the beneficial anti-obesity effects of cold studies. This is due to thermogenic activation of brown adipose tissue (BAT), a tissue also recently discovered in adult humans. Studies in humans, however, are hampered by the accessibility of most tissues. In contrast, peripheral blood mononuclear cells (PBMC) are accessible and share the expression profile of different sets of genes with other tissues, including those that reflect metabolic responses. Ferrets are an animal model physiologically closer to humans than rodents. Here, we investigated the effects on ferrets of one-week acclimation to 4 °C by analysing the PBMC transcriptome. Cold exposure deeply affected PBMC gene expression, producing a widespread down-regulation of genes involved in different biological pathways (cell cycle, gene expression regulation/protein synthesis, immune response, signal transduction, and genes related to extracellular matrix/cytoskeleton), while thermogenic and glycogenolysis-related processes were increased. Results obtained in PBMC reflected those of adipose tissue, but hardly those of the liver. Our study, using ferret as a model, reinforce PBMC usefulness as sentinel biological material for cold-exposure studies in order to deepen our understanding of the general and specific pathways affected by cold acclimation. This is relevant for future development of therapies to be used clinically.

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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8

Mediators of Inflammation ◽

10.1155/2015/629862 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Zhongsi Hong ◽

Haiyu Hong ◽

Jian Liu ◽

Xiaobin Zheng ◽

Mingxing Huang ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis B ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Luciferase Activity ◽

Peripheral Blood Mononuclear ◽

Protein Levels ◽

Qrt Pcr ◽

Blood Mononuclear Cells

Aims. This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.Materials and Methods. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.Results. The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.Conclusions. This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

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c-Jun NH2-Terminal Kinase Activity in Subcutaneous Adipose Tissue but Not Nuclear Factor- B Activity in Peripheral Blood Mononuclear Cells Is an Independent Determinant of Insulin Resistance in Healthy Individuals

Diabetes ◽

10.2337/db08-1725 ◽

2009 ◽

Vol 58 (6) ◽

pp. 1259-1265 ◽

Cited By ~ 24

Author(s):

K. C. Sourris ◽

J. G. Lyons ◽

M. P.J. de Courten ◽

S. L. Dougherty ◽

D. C. Henstridge ◽

...

Keyword(s):

Subcutaneous Adipose Tissue ◽

Kinase Activity ◽

Mononuclear Cells ◽

Nuclear Factor ◽

Healthy Individuals ◽

Peripheral Blood Mononuclear ◽

Factor B ◽

Blood Mononuclear Cells ◽

Subcutaneous Adipose ◽

Independent Determinant

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Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

International Immunopharmacology ◽

10.1016/j.intimp.2017.03.016 ◽

2017 ◽

Vol 47 ◽

pp. 59-69 ◽

Cited By ~ 17

Author(s):

Rasoul Baharlou ◽

Abbas Ahmadi-Vasmehjani ◽

Fatemeh Faraji ◽

Mohammad Reza Atashzar ◽

Mahshid Khoubyari ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Peripheral Blood Mononuclear Cells ◽

Mononuclear Cells ◽

Human Adipose Tissue ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Regulatory Effects

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Effect of high-fat diet on peripheral blood mononuclear cells and adipose tissue in early stages of diet-induced weight gain

British Journal Of Nutrition ◽

10.1017/s0007114519002472 ◽

2019 ◽

Vol 122 (12) ◽

pp. 1359-1367 ◽

Cited By ~ 1

Author(s):

Jake E. Lowry ◽

Batbayar Tumurbaatar ◽

Claudia D’Agostino ◽

Erika Main ◽

Traver J. Wright ◽

...

Keyword(s):

Adipose Tissue ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

High Fat Diet ◽

Time Course ◽

Mononuclear Cells ◽

High Fat ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Inflammatory Changes

AbstractSubcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.

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Impaired alternative macrophage differentiation of peripheral blood mononuclear cells from obese subjects

Diabetes and Vascular Disease Research ◽

10.1177/1479164111430242 ◽

2011 ◽

Vol 9 (3) ◽

pp. 189-195 ◽

Cited By ~ 31

Author(s):

Gael Bories ◽

Robert Caiazzo ◽

Bruno Derudas ◽

Corinne Copin ◽

Violeta Raverdy ◽

...

Keyword(s):

Metabolic Syndrome ◽

Adipose Tissue ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

The Metabolic Syndrome ◽

Blood Mononuclear Cells ◽

Obese Subjects ◽

Anti Inflammatory

Visceral obesity is a chronic, low-grade inflammatory disease that predisposes people to the metabolic syndrome, type 2 diabetes and its cardiovascular complications. Adipose tissue is not a passive storehouse for fat, but an endocrine organ synthesizing and releasing a variety of bioactive molecules, some of which are produced by infiltrated immune-inflammatory cells including macrophages. Two different subpopulations of macrophages have been identified in adipose tissue: pro-inflammatory ‘classical’ M1 and anti-inflammatory ‘alternative’ M2 macrophages, and their ratio is suggested to influence the metabolic complications of obesity. These macrophages derive primarily from peripheral blood mononuclear cells (PBMCs). We hypothesised that obesity and the metabolic syndrome modulate PBMC functions. Therefore, alteration of the monocyte response, and more specifically their ability to differentiate toward alternative anti-inflammatory macrophages, was assessed in PBMCs isolated from lean and obese subjects with or without alterations in glucose homeostasis. Our results indicate that PBMCs from obese subjects have an altered expression of M2 markers and that their monocytes are less susceptible to differentiate toward an alternative phenotype. Thus PBMCs in obesity are programmed, which may contribute to the inflammatory dysregulation and increased susceptibility to inflammatory diseases in these patients.

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