Predominance of CTX-M-9 Group Among ESBL-Producing Escherichia coli Isolated from Healthy Individuals in Japan

2022 ◽  
Author(s):  
Takashi Masui ◽  
Ryuichi Nakano ◽  
Akiyo Nakano ◽  
Kai Saito ◽  
Yuki Suzuki ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Healthy Individuals

Sa536 GENOME DIVERSITY OF INTESTINAL ESCHERICHIA COLI STRAINS FROM PATIENTS WITH CROHN'S DISEASE AND HEALTHY INDIVIDUALS

2021 ◽  
Vol 160 (6) ◽  
pp. S-539
Author(s):  
Maria Siniagina ◽  
Maria Markelova ◽  
Alexander Laikov ◽  
Dilyara Khusnutdinova ◽  
Eugenia A. Boulygina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Healthy Individuals ◽  
Genome Diversity

Significance of sensitization to Escherichia coli allergen in the dysentery clinic

1986 ◽  
Vol 67 (6) ◽  
pp. 419-421
Author(s):  
L. I. Badrieva
Keyword(s):  
Escherichia Coli ◽  
Clinical Course ◽  
Healthy Individuals

The purpose of this work was to study the features of the clinical course of dysentery in patients with specific and nonspecific bacterial sensitization. There were 103 patients aged from 17 to 64 years under observation: 76 patients with dysentery and 27 with influenza. Fifteen healthy individuals were examined as controls.

scholarly journals Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

2017 ◽  
Vol 66 (5) ◽  
pp. 577-583 ◽  
Author(s):  
Clarisse Oliveira-Pinto ◽  
Cristiane Diamantino ◽  
Patrícia L Oliveira ◽  
Mariana P Reis ◽  
Patrícia S Costa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Infection ◽  
Healthy Individuals ◽  
Class 1 Integrons ◽  
Class 1

scholarly journals Prevalence Of Extended Spectrum Beta-Lactamases (ESBLs)-Producing Escherichia Coli Isolated From UTI Patients Attending some Selected Hospitals In Minna, Nigeria

2021 ◽  
Vol 37 (2) ◽  
pp. 56-73
Author(s):  
F Iseghohi ◽  
J.C Igwe ◽  
M Galadima ◽  
A.F Kuta ◽  
A.M Abdullahi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Urine Samples ◽  
Antibiotics Resistance ◽  
Healthy Individuals ◽  
Beta Lactamases ◽  
E Coli ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Tract Infections

Globally, urinary tract infections are one of the most common infections in need of urgent clinical attention. The prevalence of extended spectrum beta-lactamases (ESBL)- producing Escherichia coli isolated from urine samples of some UTI patients and s of apparently healthy individuals in Minna, Nigeria, is investigated. Standard microbiological techniques were used to conduct this study. A total of 170 catch midstream urine samples submitted to the Medical Microbiology Laboratories of 4 different hospitals (and samples from healthy individuals) were randomly collected for 5 months and examined for microbial growths. Female patients (65.9%) submitted more urine samples for UTI test than their male counterpart (34.1%). The age ranges of 21 -30 (26.5%) and 31 - 40 (25.3%) had the highest percentages of infection rate while those within the ages 1- 10 (3.5%) and ≥ 71 (2.3%) were the least infected. This study observed a prevalence of 23.5% of E. coli in Minna metropolis and a significant number (30%) of healthy individuals (HI) was observed to harbor the E. coli in their urine. The isolates were highly susceptible to Gentamicin (65%), Ofloxacin (65%), Tetracycline (62.5%), Cotrimoxazole (62.5%), and Streptomycin (57.5%). Mildly susceptible to Pefloxacin (37.5%), Chloramphenicol (37.5%), and Ciprofloxacin (35%). There were significant resistance to most of the beta-lactames tested [Cefuroxime (80%), Amoxicillin (42.5%), Augmentin (40), Cefotaxime (20%) and Ceftaxidime (7.5%)]. Two of the isolates were resistant to all the 13 antibiotics tested; 70% (28) of the isolates had multiple antibiotics resistance index (MARI) ≥0.3. Multidrug resistance was expressed in 37.5% of the isolates tested. The study showed a vast resistant pool in the environment. Only 25% of the E. coli isolated from the urine samples produced beta-lactamases phenotypically, most of which expressed resistance to more than 5 of the antibiotics tested and had MARI of ≥ 0.5. Further evaluation showed that 25% (10/40) of the E. coli isolated from the UTI patients in Minna, Nigeria, were ESBL- producers and could harbor one or two of the genes. TEM gene was expressed in 70% (7) of the isolates that produced ESBL phenotypically, 60% 6) harbored CTXM gene, 20% (2) had the OXA gene while none of the bacteria harbored the SHV gene. The study established a 5.9% ESBL prevalence among the E. coli isolated from UTI in the environment studied. This study established that E. coli is one of the prevalent bacteri urea majorly isolated from UTI patients in Minna. The prevalent E. coli are multidrug resistant and could harbor more than one ESBL gene . keywords: Escherichia coli, Minna, UTI, ESBL, Multidrug resistance

scholarly journals Heterologous Expression, Purification, and Immunological Reactivity of a Recombinant HSP60 from Paracoccidioides brasiliensis

2002 ◽  
Vol 9 (2) ◽  
pp. 374-377 ◽  
Author(s):  
Daniela A. Cunha ◽  
Roseli M. Zancopé-Oliveira ◽  
M. Sueli ◽  
S. Felipe ◽  
Silvia M. Salem-Izacc ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Paracoccidioides Brasiliensis ◽  
High Sensitivity ◽  
Cross Reactivity ◽  
Antibody Responses ◽  
Healthy Individuals ◽  
Immunological Reactivity ◽  
Serum Samples ◽  
Recombinant Antigens

ABSTRACT The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysis with the purified HSP60 recombinant protein. Immunoblots indicated that the recombinant P. brasiliensis HSP60 was recognized by antibodies in 72 of 75 sera from paracoccidioidomycosis patients. No cross-reactivity was detected with individual sera from patients with aspergillosis, sporotrichosis, cryptococcosis, and tuberculosis. Reactivity to HSP60 was observed in sera from 9.52% of control healthy individuals and 11.5% of patients with histoplasmosis. The high sensitivity and specificity (97.3 and 92.5%, respectively) for HSP60 suggested that the recombinant protein can be used singly or in association with other recombinant antigens to detect antibody responses in P. brasiliensis-infected patients.

The state of the microflora of prostate secretions in healthy individuals and in acute bacterial prostatitis

2021 ◽  
Vol 25 (4) ◽  
pp. 339-345
Author(s):  
Vladimir V. Masljakov ◽  
Olga N. Pavlova ◽  
Alexandr A. Cymbal ◽  
Elena A. Pronina ◽  
Firdavshudzha P. Sultonov
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Epidermidis ◽  
Chronic Prostatitis ◽  
The State ◽  
Healthy Individuals ◽  
Bacterial Prostatitis ◽  
Comparative Aspect ◽  
Microbial Associations ◽  
Acute Bacterial Prostatitis ◽  
Klebsiella Spp

Relevance. Acute bacterial prostatitis is one of the urgent problems in urology. According to the data presented in the literature, the number of men who have been diagnosed with this disease ranges from 10 to 40 %. At the same time, the main etiological factor in the development of this pathology is most often assigned to the bacterial microflora. However, most of the works are devoted to the study of the microflora in chronic prostatitis, and the state of the microflora in acute bacterial prostatitis remains insufficient. The aim of the study is to evaluate the state of prostate secretion microflora in a comparative aspect in healthy individuals and in patients with acute bacterial prostatitis. Materials and Methods. The study included a comparative analysis of microbiological cultures in prostate secretion of 30 people. All examined were divided into two groups: the first included 15 people without established urological pathology, the second - 15 people with an established diagnosis of acute bacterial prostatitis. Results and Discussion. As a result of the study, it was found that when sowing prostate secretion in persons without established pathology, the absence of microorganism growth was not observed in 60 % of observations, while in 40 % of cases, microorganisms growth was obtained. In the group of examined without established urological pathology, the growth of microorganisms of the following was noted: Escherichia coli - in 58 % of observations; Staphylococcus epidermidis - in 10 % of observations; Staphylococcus warnerii - in 6 % cases and Enterobacter spp. - in 26 %. At the same time, the number of detected microorganisms did not exceed 104 CFU/ml. At the same time, the presence of microbial associations was not recorded. In patients with acute bacterial prostatitis, the sterile prostate secret was 13,3 %, and in 86,7 % of people, microflora growth was obtained. Most often, in the group examined with acute bacterial prostatitis, Escherichia coli was obtained - in 45 % of the observations; Klebsiella spp. - in 23 % cases and Proteus spp. - in 19 %. Less often Staphylococcus epidermidis - in 8 % of observations; Enterobacter spp. - 3,2 %. Serratia spp. - 1,1 % and Staphylococcus warnerii - in 0,7 % cases were the least obtained. At the same time, 86 % of observations in this group revealed microbial associations. Conclusion . Comparison of the state of microflora of prostate secretions in healthy individuals and patients with acute bacterial prostatitis revealed that in patients with acute bacterial prostatitis, the absence of inoculated microflora in prostate secretions was 3 times less than in healthy individuals (13.3 % and 40 %, respectively.), and Escherichia coli was the most common microorganism in the group of patients with acute bacterial prostatitis.

Antibiotic Susceptibility Profiles of Escherichia coli from Urinary Bacteruria of Asymptomatic Students of University of Ibadan

2013 ◽  
Vol 3 (5) ◽  
pp. 214-219
Author(s):  
Okunye Olufemi L ◽  
Sadiq Rukayatt ◽  
Ayedun Joshua S
Keyword(s):  
Escherichia Coli ◽  
Age Distribution ◽  
Multidrug Resistant ◽  
Healthy Individuals ◽  
Gender Ratio ◽  
Biochemical Tests ◽  
Study Region ◽  
Confirmatory Tests ◽  
Susceptibility Profiles ◽  
University Of Ibadan

Asymptomatic bacteruria is the presence of significant count bacteria capable of inducing urinary tract infection in the urine tract of clinically healthy individuals. Four hundred samples of urine were investigated for the presence of Escherichia coli in this study, out of which forty by conventional biochemical tests. The percentage prevalence gender ratio of the isolates was 77.5% females to 22.5% males while the age distribution prevalent ranged between 24 years 30years. Ten conventional biochemical confirmatory tests was carried out on the isolates. The antibiogram in this study elicited the effective potency of floroquinolones while ampicillin, a β –lactams derivatives was recorded to be less effective. Sixteen isolates showed multidrug resistant to 4 antibiotics while 2 isolates elicited multidrug resistance to 6 antibiotics in this study. The findings of this study the prevalence and the potency of selected antibiotics could be useful in the therapeutic management of asymptomatic bacteruria in the study region.

Community spread and acquisition of clinically relevant Escherichia coli harbouring blaNDM among healthy Japanese residents of Yangon, Myanmar

2021 ◽  
Author(s):  
Yo Sugawara ◽  
Hideharu Hagiya ◽  
Yukihiro Akeda ◽  
Dan Takeuchi ◽  
Noriko Sakamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Setting ◽  
Healthy Individuals ◽  
E Coli ◽  
Faecal Samples ◽  
Long Read ◽  
History Of ◽  
Japanese Residents ◽  
Retail Food

Abstract Background Carbapenemase-producing Enterobacterales (CPE) are spreading in hospitals, environment and retail foods in Yangon, Myanmar. Objectives To investigate whether CPE colonize healthy individuals living in Yangon and whether clinical-related strains are spreading in the community. Methods CPE was isolated from faecal samples obtained from healthy Japanese residents of Yangon with no history of hospitalization. Isolates were subjected to WGS using short- and long-read sequencers and compared with those previously isolated in Yangon. Results Six Escherichia coli strains harbouring blaNDM-1 or blaNDM-5 belonging to five different STs—ST10, ST38, ST48, ST410 and ST8453—were isolated from 69 volunteers. The ST38 isolates were related to those previously isolated from retail food in Yangon. The ST410 and ST8453 isolates were highly related to previous Yangon isolates including those of clinical and food origins. Conclusions The analysis suggested the acquisition of blaNDM-positive E. coli, which are disseminating in a clinical setting and through retail foods, by healthy residents in Yangon.

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