Effect of Photodynamic Therapy on Proliferation and Apoptosis of 3T3 Fibroblasts and HeLa Cells

To improve the tumor-targeting efficacy of photodynamic therapy, biotin was conjugated with chlorin e6 to develop a new tumor-targeting photosensitizer, Ce6-biotin. The Ce6-biotin had good water solubility and low aggregation. The singlet-oxygen generation rate of Ce6-biotin was slightly increased compared to Ce6. Flow cytometry and confocal laser scanning microscopy results confirmed Ce6-biotin had higher binding affinity toward biotin-receptor-positive HeLa human cervical carcinoma cells than its precursor, Ce6. Due to the BR-targeting ability of Ce6-biotin, it exhibited stronger cytotoxicity to HeLa cells upon laser irradiation. The IC50 against HeLa cells of Ce6-biotin and Ce6 were 1.28 µM and 2.31 µM, respectively. Furthermore, both Ce6-biotin and Ce6 showed minimal dark toxicity. The selectively enhanced therapeutic efficacy and low dark toxicity suggest that Ce6-biotin is a promising PS for BR-positive-tumor-targeting photodynamic therapy.

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Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage

Cells ◽

10.3390/cells10092190 ◽

2021 ◽

Vol 10 (9) ◽

pp. 2190

Author(s):

Howard Kim ◽

Mi Woon Kim ◽

Young-IL Jeong ◽

Hoe Saeng Yang

Keyword(s):

Cervical Cancer ◽

Photodynamic Therapy ◽

Hela Cells ◽

Folate Receptor ◽

Chlorin E6 ◽

Ros Generation ◽

Cervical Cancer Cells ◽

Poly Ethylene

The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (H2O2) and particle size distribution changed from monomodal distribution pattern to multimodal pattern. The fluorescence intensity and Ce6 release rate also increased due to the increase in H2O2 concentration, indicating that the nanophotosensitizers displayed ROS sensitivity. The Ce6 uptake ratio, ROS generation and cell cytotoxicity of the nanophotosensitizers were significantly higher than those of the Ce6 itself against HeLa cells in vitro. Furthermore, the nanophotosensitizers showed folate-receptor-specific delivery capacity and phototoxicity. The intracellular delivery of nanophotosensitizers was inhibited by folate receptor blocking, indicating that they have folate-receptor specificity in vitro and in vivo. Nanophotosensitizers showed higher efficiency in inhibition of tumor growth of HeLa cells in vivo compared to Ce6 alone. These results show that nanophotosensitizers of FaPEGbCDseseCe6 conjugates are promising candidates as PDT of cervical cancer.

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Mitochondria-targeted porphyrin-based photosensitizers containing triphenylphosphonium cations showing efficient in vitro photodynamic therapy effects

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619501682 ◽

2019 ◽

Vol 23 (11n12) ◽

pp. 1505-1514 ◽

Cited By ~ 1

Author(s):

Xing Guo ◽

Hao Wu ◽

Wei Miao ◽

Yangchun Wu ◽

Erhong Hao ◽

...

Keyword(s):

Photodynamic Therapy ◽

Cancer Therapy ◽

Fluorescence Spectroscopy ◽

Hela Cells ◽

Photophysical Properties ◽

Quantum Yields ◽

Subcellular Organelle

Subcellular organelle-targeted photosensitizers have recently reported to be effective photodynamic therapy (PDT) agents. In this work, three porphyrin-derived photosensitizers, containing one, two or four triphenylphosphonium targeting groups, were synthesized and characterized by NMR, HRMS, UV-vis and fluorescence spectroscopy. These photosensitizers showed similar photophysical properties to classical porphyrins and exhibited excellent [Formula: see text]O[Formula: see text] quantum yields in acetonitrile. Subcellular colocalization indicated that all three photosensitizers specifically stain the mitochondria of HeLa cells. Photosensitizer mito-dp, containing two triphenylphosphonium cations was found to be the most uptaken by cells and exhibited the best PDT effect with an effective phototoxicity (IC[Formula: see text] (light) [Formula: see text] 12.4 nM), suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.

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Photodynamic Therapy Enhanced the Antitumor Effects of Berberine on HeLa Cells

Open Chemistry ◽

10.1515/chem-2019-0048 ◽

2019 ◽

Vol 17 (1) ◽

pp. 413-421 ◽

Cited By ~ 3

Author(s):

Han-Qing Liu ◽

Ya-Wen An ◽

A-Zhen Hu ◽

Ming-Hua Li ◽

Guang-Hui Cui

Keyword(s):

Photodynamic Therapy ◽

Western Blot ◽

Hela Cells ◽

Mammalian Target Of Rapamycin ◽

Control Group ◽

Antitumor Effects ◽

Underlying Mechanisms ◽

And Control

AbstractIn this study we investigated the antineoplastic effects of Berberine (BBR)-mediated photodynamic therapy (PDT) on HeLa cells and its related mechanisms. The CCK-8 assay and flow cytometry were used to evaluate the proliferation and apoptosis of cells respectively. In addition, changes in protein expression levels were assessed using western blot. BBR at dose of 10 mg/kg was injected intraperitoneally to mice with tumors and PDT treatments were performed 24 hours later. In vivo imaging systems were used to evaluate the fluorescence of BBR. In vitro, PDT significantly enhanced the effects of BBR on inducing cell apoptosis and inhibiting proliferation. The in vivo results showed that the fluorescence intensity in the PDT group was decreased compared with that in the BBR group. Tumor weights and tumor size in the PDT group were less than those in the control group; however, when BBR was applied without PDT, no significant differences were observed between the BBR and control group. The results of western blot showed that PDT enhanced the inhibitory effects of BBR on the mammalian target of rapamycin (mTOR) signaling pathway, that may partly explain the potential underlying mechanisms.

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Synthesis, photophysical properties and in vitro evaluation of a chlorambucil conjugated ruthenium(ii) complex for combined chemo-photodynamic therapy against HeLa cells

Journal of Materials Chemistry B ◽

10.1039/c7tb00702g ◽

2017 ◽

Vol 5 (24) ◽

pp. 4623-4632 ◽

Cited By ~ 9

Author(s):

Jing-Xiang Zhang ◽

Mei Pan ◽

Cheng-Yong Su

Keyword(s):

Photodynamic Therapy ◽

Hela Cells ◽

Photophysical Properties ◽

Inhibitory Effect ◽

Antiproliferative Effect ◽

In Vitro Evaluation

We designed a new heteroleptic Ru(ii) complex CHL-RuL as an imaging-guided chemotherapy/PDT agent, which shows a moderate antiproliferative effect in dark and strong photodynamic inhibitory effect against HeLa cells.

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EFFECT OF 630-NM PULSED LASER IRRADIATION ON THE PROLIFERATION OF HeLa CELLS IN PHOTOFRIN-MEDIATED PHOTODYNAMIC THERAPY

LASER THERAPY ◽

10.5978/islsm.20.135 ◽

2011 ◽

Vol 20 (2) ◽

pp. 135-138 ◽

Cited By ~ 6

Author(s):

Yuichi Miyamoto ◽

Daisuke Nishikiori ◽

Fumika Hagino ◽

Masayoshi Wakita ◽

Ichiro Tanabe ◽

...

Keyword(s):

Photodynamic Therapy ◽

Laser Irradiation ◽

Hela Cells ◽

Pulsed Laser ◽

Pulsed Laser Irradiation

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The photodynamic therapy activity of 3-(1-hydroxylethyl)-3-devinyl-131-(dicyanomethylene) pyropheophorbide-a methyl ester (HDCPPa) against HeLa cell in vitro

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424617500584 ◽

2017 ◽

Vol 21 (09) ◽

pp. 589-598 ◽

Cited By ~ 2

Author(s):

Wenting Li ◽

Qi Wang ◽

Guanghui Tan ◽

Hongyue Zhang ◽

Jianjun Cheng ◽

...

Keyword(s):

Photodynamic Therapy ◽

Methyl Ester ◽

Hela Cells ◽

High Stability ◽

Fluorescence Emission ◽

Ultraviolet Absorption Spectrum ◽

Excitation Wavelength ◽

Fluorescence Emission Spectrum ◽

Pyropheophorbide A

Photodynamic therapy (PDT) has been a potential therapeutic method for the treatment of various cancers, with photosensitizer being the key component in photodynamic therapy. In this paper, we prepared a photosensitizer 3-(1-hydroxylethyl)-3-devinyl-131-(dicyanomethylene) pyropheophorbide-a methyl ester (HDCPPa), based on chlorophyll pyropheophorbide-a according to the previous report, and systematically investigated the fluorescence emission spectrum and ultraviolet absorption spectrum HDCPPa has long absorption in the near-infrared spectral region (around 695 nm). The excitation wavelength and the emission wavelength were 415 nm and 699 nm respectively in dichloromethane, 1O2 quantum yield was 63.5%. HDCPPa also had high stability in PBS solution, DMEM cell culture medium and normal saline (NS) in vitro. After irradiation by the light of 675 nm (10 J.cm[Formula: see text]) for 70 min the degradation rate of HDCPPa was 12.5%, which indicated that the target compound showed high stability under light. The in vitrophotodynamic therapy activities against HeLa cells were also studied, which showed that HDCPPa had extremely low dark toxicity but great phototoxicity, and the cell viability is lower than 10% under the light irradiation of 675 nm (10 J.cm[Formula: see text]). Moreover, HDCPPa can quickly enter the cell after being incubated with HeLa cells in less than 30 min. We also evaluated the mechanism of the photochemical reaction, which had proved that Type II is primarily responsible for the cell death. Therefore HDCPPa could serve as a very promising photosensitizer for photodynamic therapy.

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Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/568932 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 18

Author(s):

Carla Oliveira ◽

Ana Nicolau ◽

José A. Teixeira ◽

Lucília Domingues

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Hela Cells ◽

Carbohydrate Binding ◽

Binding Affinities ◽

Proliferation And Apoptosis ◽

Cervical Cancer Cells ◽

Different Sources ◽

Binding Lectin ◽

Galactose Binding Lectin

Frutalin is theα-D-galactose-binding lectin isolated from breadfruit seeds. Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified fromPichia pastoris. This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis. Furthermore, the interaction between frutalin and the HeLa cells was investigated by confocal microscopy. Despite having different carbohydrate-binding affinities, native and recombinant frutalin showed an identical magnitude of cytotoxicity on HeLa cells growth (IC50~100 μg/mL) and equally induced cell apoptosis. The interaction studies showed that both lectins were rapidly internalised and targeted to HeLa cell's nucleus. Altogether, these results indicate that frutalin action is not dependent on its sugar-binding properties. This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity.

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