Biologically derived, 3-dimensional, embryonic scaffolds for long-term cardiomyocyte culture

2021 ◽  
Author(s):  
Mary G Garry ◽  
Stefan Kren ◽  
Joseph B Wenger ◽  
Daniel J. Garry
Keyword(s):  
3 Dimensional ◽  
Cardiomyocyte Culture

scholarly journals An ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Marisa Nacke ◽  
Emma Sandilands ◽  
Konstantina Nikolatou ◽  
Álvaro Román-Fernández ◽  
Susan Mason ◽  
...  
Keyword(s):  
Metastatic Cancer ◽  
Cellular Responses ◽  
Signalling Pathways ◽  
External Signal ◽  
Phosphoinositide Metabolism ◽  
Invasion And Metastasis ◽  
3 Dimensional

AbstractThe signalling pathways underpinning cell growth and invasion use overlapping components, yet how mutually exclusive cellular responses occur is unclear. Here, we report development of 3-Dimensional culture analyses to separately quantify growth and invasion. We identify that alternate variants of IQSEC1, an ARF GTPase Exchange Factor, act as switches to promote invasion over growth by controlling phosphoinositide metabolism. All IQSEC1 variants activate ARF5- and ARF6-dependent PIP5-kinase to promote PI(3,4,5)P3-AKT signalling and growth. In contrast, select pro-invasive IQSEC1 variants promote PI(3,4,5)P3 production to form invasion-driving protrusions. Inhibition of IQSEC1 attenuates invasion in vitro and metastasis in vivo. Induction of pro-invasive IQSEC1 variants and elevated IQSEC1 expression occurs in a number of tumour types and is associated with higher-grade metastatic cancer, activation of PI(3,4,5)P3 signalling, and predicts long-term poor outcome across multiple cancers. IQSEC1-regulated phosphoinositide metabolism therefore is a switch to induce invasion over growth in response to the same external signal. Targeting IQSEC1 as the central regulator of this switch may represent a therapeutic vulnerability to stop metastasis.

scholarly journals Predictors of Success in Ablation of Scar-Related Ventricular Tachycardia

2013 ◽  
Vol 7 ◽  
pp. CMC.S11501 ◽  
Author(s):  
Mazen T. Ghanem ◽  
Rania S. Ahmed ◽  
Ayman M. Abd El Moteleb ◽  
John K. Zarif
Keyword(s):  
Ventricular Tachycardia ◽  
Univariate Analysis ◽  
Scar Tissue ◽  
Focal Lesions ◽  
3 Dimensional ◽  
Linear Lesions ◽  
Linear Ablation ◽  
Dimensional Mapping ◽  
Mapping Systems

During ablation of re-entrant ventricular tachycardia (VT) 3-dimensional mapping systems are now used to properly delineate the scar tissue and aid ablation of scar-related VT. The aim of our study was to outline how the mode of ablation predicts success and recurrence in large scar-related VT. When comparing patients with recurrence and patients with no recurrence, univariate analysis showed that number of ablation lesions (28 ± 8 vs. 12 ± 8, P = 0.01) and more linear ablation lesions rather than focal lesions ( P = 0.03) were associated with long-term success. We demonstrated that more extensive ablation lesions and creation of linear lesions is associated with better success rate and lower recurrence rate during ablation of large scar-related ventricular tachycardia.

Does the Body Image Exist in Three Dimensions? The Study of Visual Mental Representation of a Body and a Nonbody Object

2001 ◽  
Vol 92 (1) ◽  
pp. 223-233
Author(s):  
D. P. McCabe ◽  
D. I. Ben-Tovim ◽  
M. K. Walker ◽  
D. Pomeroy
Keyword(s):  
Spatial Information ◽  
Mental Image ◽  
The Body ◽  
Three Dimensions ◽  
Long Term Memory ◽  
Depth Information ◽  
Mental Images ◽  
Term Memory ◽  
3 Dimensional

Do the mental Images of 3-dimensional objects recreate the depth characteristics of the original objects' This investigation of the characteristics of mental images utilized a novel boundary-detection task that required participants to relate a pair of crosses to the boundary of an image mentally projected onto a computer screen. 48 female participants with body attitudes within expected normal range were asked to image their own body and a familiar object from the front and the side. When the visual mental image was derived purely from long-term memory, accuracy was better than chance for the front (64%) and side (63%) of the body and also for the front (55%) and side (68%) of the familiar nonbody object. This suggests that mental images containing depth and spatial information may be generated from information held in long-term memory. Pictorial exposure to views of the front or side of the objects was used to investigate the representations from which this 3-dimensional shape and size information is derived. The results are discussed in terms of three possible representational formats and argue that a front-view 2½-dimensional representation mediates the transfer of information from long-term memory when depth information about the body is required.

scholarly journals Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Sung-Hwan Moon ◽  
Young-Woo Cho ◽  
Hye-Eun Shim ◽  
Jae-Hak Choi ◽  
Chan-Hee Jung ◽  
...  
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Cardiomyocyte Culture

Long-term results of 3-dimensional conformal radiation therapy for rhabdomyosarcoma

2002 ◽  
Vol 54 (2) ◽  
pp. 151
Author(s):  
T.H La ◽  
L.H Wexler ◽  
M.P LaQuaglia ◽  
P.A Meyers ◽  
D.H Kraus ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Long Term Results ◽  
Conformal Radiation Therapy ◽  
3 Dimensional

Advantages and Disadvantages of the Use of Various Types of Interbody Implants in Cervical Spine Surgery. Critical Review of the Literature

2020 ◽  
Vol 22 (4) ◽  
pp. 213-222
Author(s):  
Bartosz Godlewski( ◽  
Maciej Dominiak
Keyword(s):  
Cervical Spine ◽  
Spine Surgery ◽  
Structural Design ◽  
Titanium Implants ◽  
Implant Fixation ◽  
Disc Disease ◽  
Cervical Spine Surgery ◽  
3 Dimensional ◽  
Advantages And Disadvantages

Background. Most cervical spine procedures in patients with degenerative disc disease involve discectomy and remo­val of osteophytes in posterior vertebral body surfaces followed by interbody stabilisation with an interbody implant. Interbody implants are made of a variety of materials, differing in structural design, shape and surface topography. Considering that fusion between the implant and host bone is crucial for long-term positive outcomes, the choice of an appropriate implant is significantly important clinically and continues to be an important area of study. Material and methods. Relevant published studies indexed by Medline were identified via PubMed and reviewed. The findings were combined with the authors’ experiences. The database query was based on keywords related to implants in cervical spine surgery. This article presents the currently most popular types of implants by describing their properties and indicating their strengths and weaknesses as well as differences between different implant types. Results. Currently, the most popular interbody cages in cervical spine surgery are polyetheretherketone (PEEK) im­plants, titanium-coated PEEK implants and titanium implants. Besides the type of material used, the shape and surface structure of an implant appear to be of significant importance for a successful bony fusion. Conclusions. 1. 3D printing and the ability to produce 3-dimensional porous-surfaced implants opens up considerable pro­spects for this technique in the production of modern interbody implants. 2. Implants that facilitate the engagement (interlocking) of greater volumes of bone (e.g. porous implants) offer better implant fixation, with the type of material used being less important.

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