Воздействие гармонического сигнала на генератор квазипериодических колебаний Анищенко-Астахова

A harmonic effect on a modified Anishchenko-Astakhov generator capable of demonstrating two-frequency quasi-periodic oscillations in the autonomous mode is considered. The possibility of doubling the three-frequency tori in a non-autonomous system is shown. The possibility of the effect of chaos suppression by an external signal is demonstrated, which leads not only to periodic, but also to quasi-periodic modes when the influence amplitude exceeds a certain threshold.

Methodology of speed qualities development of police officers for actions in extreme situations of operational activities

The article considers the scientific foundations of the pedagogical approach to the study and development of speed qualities, in particular, the reaction speed and functional capacities of a person who provides professional training of police officers and athletes. During the research, the features of the speed qualities development, in particular the speed of reaction, were studied by means of physical training, with the help of which it is possible to achieve maximum speed of response, and, on this basis, to form stable skills for counteraction in emergency situations related to service-applied activities. A set of factors determining the speed of response to an external signal is considered. Methods for the growth of speed in the training process serve as the basis for the progress of this quality in relation to the operational and service activities of police officers. The article presents the most effective methods of speed development, aimed both at improving this physical quality, and contributing to the formation of the necessary motor skills for the successful fulfillment of operational-service tasks and for competing. Speed qualities are complex in their structure, including the time of the motor reaction, the speed of a single movement, the frequency of movements, etc. These complexity and diversity should be taken into account during the training of high-speed abilities of police officers for actions in extreme situations of operational and service activities, and a special emphasis on the acquisition and development of new motor skills and abilities should be made.

Global Analysis of The Signalling Network of Breast Cancer Cells In Response To Progesterone.

Background: Breast cancer cells enter into the cell cycle following progestin exposure by the activation of signalling cascades involving a plethora of enzymes, transcription factors and co-factors that transmit the external signal from the cell membrane to chromatin, ultimately leading to a change of the gene expression program. Although many of the events within the signalling network have been described in isolation, how they globally team up to generate the final cell response is unclear. Methods: In this study we used antibody microarrays and phosphoproteomics to reveal a dynamic global signalling map that reveals new key regulated proteins and phosphor-sites and links between previously known and novel pathways. T47D breast cancer cells were used, and phosphosites and pathways highlighted were validated using specific antibodies and phenotypic assays. Bioinformatic analysis revealed an enrichment in novel signalling pathways, a coordinated response between cellular compartments and protein complexes. Results: Detailed analysis of the data revealed intriguing changes in protein complexes involved in nuclear structure, epithelial to mesenchyme transition (EMT), cell adhesion, as well as transcription factors previously not associated with breast cancer proliferation. Pathway analysis confirmed the key role of MAPK following progesterone and additional hormone regulated phosphosites were identified. Full network analysis shows the activation of new signalling pathways previously not associated with progesterone signalling in breast cancer cells such as ERBB and TRK. As different post-translational modifications can mediate complex crosstalk mechanisms and massive PARylation is also rapidly induced by progestins, we provide details of important chromatin regulatory complexes containing both phosphorylated and PARylated proteins. Conclusions: This study contributes an important resource for the scientific community, as it identifies novel players and connections meaningful for breast cancer cell biology and potentially relevant for cancer management.

Interconnection of phospholipolysis and monooxygenase catalysis

A review of the main experimental results in the field of studying the interaction in vivo and in vitro of enzyme systems responsible for the metabolism of arachidonic acid is presented. Metabolic events from its release from phospholipids (phospholipase A2) to its transformation (cytochromes P450) into the most important intracellular messengers of external signal transmission to the internal “language of the cell” are discussed.

Study of gyrotron synchronization by an external harmonic signal based on a modified quasi-linear theory

Topic. The paper is devoted to the study of synchronization of a gyrotron by an external harmonic signal. A theoretical study of gyrotron synchronization processes by means of a computational experiment based on certain traditional models of microwave electronics does not provide a complete description of the synchronization pattern. Therefore, the goal of the paper is to develop a modified quasi-linear model based on an approximation of the electron susceptibility by rational functions. Methods. The developed model allows for bifurcation analysis of synchronization processes. On its basis, stationary states are determined and their stability analysis is carried out. The results are in good agreement with numerical simulation based on the non-stationary theory of a gyrotron with a fixed Gaussian high-frequency field structure. Results and discussion. Resonance curves and synchronization bounds are built on the plane of parameters “amplitude – frequency of external signal”. The case where the gyrotron is in the hard excitation mode is considered, since the maximum efficiency is usually achieved in the hard excitation mode. In general, the results are in qualitative agreement with the picture described earlier for a simpler quasi-linear model of a oscillator with hard excitation, in the case of a sufficiently strong phase nonlinearity.

Nonmonotonous temperature dependence of Shapiro steps in YBCO grain boundary junctions

The amplitudes of the first Shapiro steps for an external signal with frequencies of 72 and 265 GHz are measured as function of the temperature from 20 to 80 K for a 6 μm Josephson grain boundary junction fabricated by YBaCuO film deposition on an yttria-stabilized zirconia bicrystal substrate. Non-monotonic dependences of step heights for different external signal frequencies were found in the limit of a weak driving signal, with the maxima occurring at different points as function of the temperature. The step heights are in agreement with the calculations based on the resistively–capacitively shunted junction model and Bessel theory. The emergence of the receiving optima is explained by the mutual influence of the varying critical current and the characteristic frequency.

Epilepsy and Ecstatic Experiences: The Role of the Insula

Ecstatic epilepsy is a rare form of focal epilepsy in which the aura (beginning of the seizures) consists of a blissful state of mental clarity/feeling of certainty. Such a state has also been described as a “religious” or mystical experience. While this form of epilepsy has long been recognized as a temporal lobe epilepsy, we have accumulated evidence converging toward the location of the symptomatogenic zone in the dorsal anterior insula during the 10 last years. The neurocognitive hypothesis for the genesis of a mental clarity is the suppression of the interoceptive prediction errors and of the unexpected surprise associated with any incoming internal or external signal, usually processed by the dorsal anterior insula. This mimics a perfect prediction of the world and induces a feeling of certainty. The ecstatic epilepsy is thus an amazing model for the role of anterior insula in uncertainty and surprise.

Chromatin changes in PIF-regulated genes parallel their rapid transcriptional response to light

As sessile organisms, plants must adapt to a changing environment, sensing variations in resource availability and modifying their development in response. Light is one of the most important resources for plants, and its perception by sensory photoreceptors (e.g. phytochromes) and subsequent transduction into long-term transcriptional reprogramming have been well characterized. Chromatin changes have been shown to be involved in photomorphogenesis. However, the initial short-term transcriptional changes produced by light and what factors enable these rapid changes are not well studied. Here, we identify rapidly light-responsive, PIF (Phytochrome Interacting Factor) direct-target genes (LRP-DTGs). We found that a majority of these genes also show rapid changes in Histone 3 Lysine-9 acetylation (H3K9ac) in response to the light signal. Detailed time-course analysis of transcriptional and chromatin changes showed that, for light-repressed genes, H3K9 deacetylation parallels light-triggered transcriptional repression, while for light-induced genes, H3K9 acetylation appeared to somewhat precede light-activated transcription. However, real-time imaging of transcription elongation revealed that, in fact, H3K9 acetylation also parallels transcriptional induction. Collectively, the data raise the possibility that light-induced transcriptional and chromatin-remodeling processes are mechanistically intertwined. Histone modifying proteins involved in long term light responses do not seem to have a role in this fast response, indicating that different factors might act at different stages of the light response. This work not only advances our understanding of plant responses to light, but also unveils a system in which rapid chromatin changes in reaction to an external signal can be studied under natural conditions.

An English Mobile Learning Platform Embedded in GSM-R Wireless Network Communication

English mobile learning platform is affected by external interference signals, resulting in poor remote transmission ability of English information. To solve this problem, a design of the English mobile learning platform based on the GSM-R wireless network communication system is proposed. The overall framework of the English mobile learning platform is designed. MSP430F149 single-chip microcomputer is selected as the hardware microcontrol unit of the platform, and R-C reset circuit is designed. The reset circuit is used to reset the single-chip microcomputer platform to prevent external signal interference. The modem is initialized according to AT instruction, and the main program flow of the single-chip microcomputer of the English mobile learning platform is designed on this basis. GSM-R wireless network communication system is used to plan the course module. After planning, the platform operation management function is designed, the interactive learning environment is established, the user information template is designed, and the platform database is built. The experimental results show that the designed English mobile learning platform can meet the learning needs and improve students’ comprehensive ability after application.

Inflammatory cytokines TNFα, IL-1β, and IL-6 are induced in endotoxin- stimulated microglia through different signaling cascades

By using an animal model in which inflammatory cytokines are induced in lipopolysaccharide (LPS)-injected rat brain, we investigated the induction of tumor necrosis factor alpha (TNFα), interleukin-1beta (IL-1β), and IL-6. Immunoblotting and immunohistochemistry revealed that all three cytokines were transiently induced in the cerebral cortex at about 12 h after LPS injection. To clarify which glial cell type induced the cytokines, we examined the respective abilities of astrocytes and microglia in vitro. Primary microglia largely induced TNFα, IL-1β and IL-6 in response to LPS, but primary astrocytes induced only limited levels of TNFα. Thus, we used specific inhibitors to focus on microglia in surveying signaling molecules involved in the induction of TNFα, IL-1β, and IL-6. The experiments using mitogen-activated protein kinases (MAPK) inhibitors revealed that c-Jun N-terminal kinase (JNK)/p38, external signal regulated kinase (ERK)/JNK, and ERK/JNK/p38 are necessary for the induction of TNFα, IL-1β, and IL-6, respectively. The experiments using protein kinase C (PKC) inhibitor clarified that PKCα is required for the induction of all these cytokines in LPS-stimulated microglia. Furthermore, LPS-dependent IL-1β/IL-6 induction was suppressed by pretreatment with a nitric oxide (NO) scavenger, suggesting that NO is involved in the signaling cascade of IL-1β/IL-6 induction. Thus, an inducible NO synthase induced in the LPS-injected cerebral cortex might be related to the induction of IL-1β/IL-6 through the production of NO in vivo. Taken together, these results demonstrated that microglia induce different kinds of inflammatory cytokine through specific combinations of MAPKs and by the presence or absence of NO.