Inhibitory effects of plasma dialysate on protein synthesis in vitro: influence of dialysis and transplantation

1980 ◽  
Vol 33 (7) ◽  
pp. 1407-1410 ◽  
Author(s):  
C Delaporte ◽  
F Gros ◽  
T Anagnostopoulos
Keyword(s):  
Protein Synthesis ◽  
Inhibitory Effects

Inhibitory effects of histidine analogues on growth and protein synthesis by Plasmodium falciparum in vitro

1986 ◽  
Vol 35 (9) ◽  
pp. 1589-1596 ◽  
Author(s):  
Russell J. Howard ◽  
Annette T. Andrutis ◽  
James H. Leech ◽  
William Y. Ellis ◽  
Louis A. Cohen ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Synthesis ◽  
Inhibitory Effects

Effects of immunosuppressive drugs on secondary antibody response in vitro

1968 ◽  
Vol 14 (1) ◽  
pp. 7-11 ◽  
Author(s):  
F. C. Leung ◽  
S. I. Vas
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Immunosuppressive Agent ◽  
Immunosuppressive Drugs ◽  
Tissue Cultures ◽  
Secondary Antibody ◽  
Inhibitory Effects ◽  
Antibody Synthesis ◽  
Relative Inhibition

The inhibitory effects of 6-mercaptopurine, Imuran, and chloramphenicol on the in vitro incorporation of radioactive amino acid into antibody have been investigated. Inhibition of antibody synthesis by these drugs was compared to inhibition of protein synthesis within the same tissue cultures. The relative inhibition of antibody synthesis compared to that of protein synthesis provided an index which indicated the specificity and toxicity of the immunosuppressive agent. Imuran, at concentrations of 10–20 μg/ml inhibited antibody synthesis primarily, while at higher concentrations, protein synthesis was similarly affected. 6-Mercaptopurine at 177–250 μg/ml inhibited antibody production to a certain degree, while chloramphenicol at 300 μg/ml showed no preferential inhibition of antibody synthesis.

Evidence for the Importance of Puromycyl Peptides in the Inhibition by Puromycin of Cell Aggregation In Vitro

1973 ◽  
Vol 12 (2) ◽  
pp. 641-653
Author(s):  
M. J. DUNN ◽  
E. OWEN ◽  
R. B. KEMP
Keyword(s):  
Carbon Dioxide ◽  
Protein Synthesis ◽  
Cell Aggregation ◽  
Experimental Period ◽  
Respiratory Metabolism ◽  
Inhibitory Effects ◽  
Carbon Dioxide Evolution ◽  
Dissociated Cells ◽  
Cellular Oxygen

Trypsin-dissociated cells from the muscle tissue of 9-day-old chick embryos were employed to investigate the effects of cycloheximide and a puromycin-cycloheximide mixture on cell aggregation, protein synthesis and respiratory metabolism. Cycloheximide when introduced at a concentration of 10 µg/ml into a suspension of cells in Eagle's MEM inhibited aggregation by 25% at 24 h. At this time an inhibition of 40% was apparent in the presence of a mixture of cycloheximide and puromycin both at a concentration of 10 µg/ml. Both cycloheximide and the cycloheximide-puromycin mixture arrested protein synthesis of rotated cells by 90% within 15 min of introducing the antibiotics into cell suspensions. The antibiotics retained their inhibitory effects on protein synthesis for the 24-h period of rotation. Cycloheximide inhibited cellular oxygen uptake and carbon dioxide evolution of rotated cells by 25% at the end of the 24-h experimental period. At this time an inhibition of 30% was observed in the presence of the cycloheximide-puromycin mixture. The release of radioactive carbon dioxide by cycloheximide-treated cells was inhibited by 46% at 24 h. In the presence of the antibiotic mixture, 14CO2 release was inhibited by 30% at 4 h, but after 8 h very little further 14CO2 was evolved. As a control, puromycin (10 µg/ml) inhibited cell aggregation and respiration to an extent similar to that previously reported. The results are discussed in terms of puromycyl peptides producing a metabolic effect on cell aggregation. It is considered that this is additional to the effect of puromycin inhibiting aggregation through the arrest of protein synthesis.

scholarly journals Effects of Progesterone on the Oestrogen-Stimulated Uterus: a Comparative Study of the Mouse, Guinea Pig, Rabbit and Sheep

1979 ◽  
Vol 32 (6) ◽  
pp. 549 ◽  
Author(s):  
BG Miller ◽  
Jennifer Wild ◽  
GM Stone
Keyword(s):  
Protein Synthesis ◽  
Guinea Pig ◽  
Comparative Study ◽  
Peroxidase Activity ◽  
Species Differences ◽  
Inhibitory Effects ◽  
Dna And Rna ◽  
Wet Weight ◽  
Vitro Protein

To examine more closely the anti-oestrogenic action of progesterone (P), its effect on various parameters in the 17 fl-oestradiol (E2)-primed uterus of the mouse, guinea pig, rabbit and ewe was studied. Changes in uterine wet weight, rate of in vitro protein synthesis, protein: DNA and RNA :. DNA ratios, peroxidase activity and the level of cytosol receptors for E2 and P were measured. Considerable between-species differences in the effect of P on these parameters were observed. The anti-uterotrophic action was greater in the mouse than in the guinea pig and was not seen in the rabbit or ewe. P inhibited protein synthesis in the mouse, was without significant effect in the guinea pig and was mildly stimulatory in the rabbit and ewe. Inhibitory effects on protein: DNA and RNA: DNA ratios were substantial in the mouse, minor in the guinea pig and absent in the rabbit and ewe. Peroxidase activity was decreased in the mouse and guinea pig, essentially lacking in the rabbit and not detectable in the ewe. In all species the level of both oestrogen and progesterone cytosol receptors was decreased, although the effect on the E2 receptor was less marked in the ewe.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

Inhibitory effects of epimedium herb water extract on the inflammatory response in vitro and in vivo

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
YC Oh ◽  
YH Jeong ◽  
WK Cho ◽  
SJ Lee ◽  
JY Ma
Keyword(s):  
Inflammatory Response ◽  
Water Extract ◽  
Inhibitory Effects

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

1976 ◽  
Vol 35 (02) ◽  
pp. 350-357 ◽  
Author(s):  
Hana Bessler ◽  
Galila Agam ◽  
Meir Djaldetti
Keyword(s):  
Protein Synthesis ◽  
Fold Increase ◽  
Human Platelets ◽  
Polystyrene Latex ◽  
Latex Particles ◽  
The Third ◽  
Platelet Protein ◽  
Dose Dependent ◽  
Phagocytotic Activity

SummaryA three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

The Effects of Brinolase (Fibrinolytic Enzyme from Aspergillus Oryzae) on Platelet Aggregation of Dog and Man

1972 ◽  
Vol 28 (01) ◽  
pp. 031-048 ◽  
Author(s):  
W. H. E Roschlau ◽  
R Gage
Keyword(s):  
Platelet Aggregation ◽  
Aspergillus Oryzae ◽  
Degradation Products ◽  
Blood Platelet ◽  
Human Platelets ◽  
Fibrinolytic Enzyme ◽  
Inhibitory Effects ◽  
Blood Platelet Aggregation

SummaryInhibition of blood platelet aggregation by brinolase (fibrinolytic enzyme from Aspergillus oryzae) has been demonstrated with human platelets in vitro and with dog platelets in vivo and in vitro, using both ADP and collagen as aggregating stimuli. It is suggested that the optimal inhibitory effects of brinolase occur indirectly through the generation of plasma fibrinogen degradation products, without compromising platelet viability, rather than by direct proteolysis of platelet structures.

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