scholarly journals Markedly Reduced Azithromycin and Ceftriaxone Susceptibility in Commensal Neisseria Species in Clinical Samples From Belgian Men Who Have Sex With Men

2020 ◽  
Author(s):  
Jolein G E Laumen ◽  
Christophe Van Dijck ◽  
Said Abdellati ◽  
Sheeba S Manoharan-Basil ◽  
Irith De Baetselier ◽  
...  
Keyword(s):  
Clinical Samples ◽  
Neisseria Species ◽  
Sex With Men ◽  
Commensal Neisseria

scholarly journals Decreased Cephalosporin Susceptibility of Oropharyngeal Neisseria Species in Antibiotic-using Men Who Have Sex With Men in Hanoi, Vietnam

2019 ◽  
Vol 70 (6) ◽  
pp. 1169-1175 ◽  
Author(s):  
Huan V Dong ◽  
Loc Q Pham ◽  
Hoa T Nguyen ◽  
Minh X B Nguyen ◽  
Trung V Nguyen ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Neisseria Gonorrhoeae ◽  
Genetic Material ◽  
Antibiotic Use ◽  
Neisseria Species ◽  
The Past ◽  
User Groups ◽  
Sex With Men ◽  
Imminent Threat ◽  
Commensal Neisseria

Abstract Background Neisseria gonorrhoeae (NG) infections are a global health burden. NG resistance to cephalosporins, which is increasingly reported, is an imminent threat to public health. Many hypothesize that commensal Neisseria species are an important reservoir for genetic material conferring antimicrobial resistance in NG; however, clinical data are lacking. Methods Men who have sex with men (MSM) in Hanoi, Vietnam, completed a questionnaire regarding antibiotic use. We collected pharyngeal specimens, cultured Neisseria species, and measured minimum inhibitory concentrations (MICs) to ciprofloxacin, cefixime, ceftriaxone, and cefpodoxime. Using MIC criteria for antimicrobial susceptibility in NG, we categorized the Neisseria species and compared mean MIC levels between different antibiotic user groups. Results Of 207 participants, 38% used at least 1 antibiotic in the past 6 months; 52% without a prescription. A median of 1 Neisseria species was cultured from each participant (range, 1–4) with 10 different Neisseria species identified overall. The proportion of Neisseria with reduced susceptibility to ciprofloxacin was 93%, cefpodoxime 84%, cefixime 31%, and ceftriaxone 28%. Antibiotic use within the past month was strongly associated with Neisseria species having increased MICs to cefixime, ceftriaxone, and cefpodoxime (mean MIC ratios of 6.27, 4.11, and 7.70, respectively), compared with those who used antibiotics between 1 and 6 months prior (P < .05, all comparisons). Conclusions MSM in our study often used antibiotics without a prescription. At least 1 commensal Neisseria species colonized all men. Recent use of any antibiotics may select for oropharyngeal Neisseria species with antimicrobial resistance. The normal flora of the oropharynx may be an important source of antimicrobial resistance in Neisseria gonorrhoeae.

Comparison of Men Who Have Sex with Men in Clinical Samples with MSM in a Community Probability Sample

2008 ◽  
Author(s):  
Devon D. Brewer ◽  
Matthew R. Golden ◽  
Robert W. Wood ◽  
Barbara Krekeler ◽  
Hunter H. Handsfield
Keyword(s):  
Clinical Samples ◽  
Probability Sample ◽  
Sex With Men

scholarly journals WGS of Commensal Neisseria Reveals Acquisition of a New Ribosomal Protection Protein (MsrD) as a Possible Explanation for High Level Azithromycin Resistance in Belgium

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 384
Author(s):  
Tessa de Block ◽  
Jolein Gyonne Elise Laumen ◽  
Christophe Van Dijck ◽  
Said Abdellati ◽  
Irith De Baetselier ◽  
...  
Keyword(s):  
Integration Site ◽  
Bacterial Species ◽  
Resistance Mechanism ◽  
Health Concern ◽  
Sex With Men ◽  
High Level ◽  
Ribosomal Protection Protein ◽  
Commensal Neisseria ◽  
Level Resistance ◽  
Ribosomal Protection

In this study, we characterized all oropharyngeal and anorectal isolates of Neisseria spp. in a cohort of men who have sex with men. This resulted in a panel of pathogenic Neisseria (N. gonorrhoeae [n = 5] and N. meningitidis [n = 5]) and nonpathogenic Neisseria (N. subflava [n = 11], N. mucosa [n = 3] and N. oralis [n = 2]). A high proportion of strains in this panel were resistant to azithromycin (18/26) and ceftriaxone (3/26). Whole genome sequencing (WGS) of these strains identified numerous mutations that are known to confer reduced susceptibility to azithromycin and ceftriaxone in N. gonorrhoeae. The presence or absence of these known mutations did not explain the high level resistance to azithromycin (>256 mg/L) in the nonpathogenic isolates (8/16). After screening for antimicrobial resistance (AMR) genes, we found a ribosomal protection protein, Msr(D), in these highly azithromycin resistant nonpathogenic strains. The complete integration site originated from Streptococcus pneumoniae and is associated with high level resistance to azithromycin in many other bacterial species. This novel AMR resistance mechanism to azithromycin in nonpathogenic Neisseria could be a public health concern if it were to be transmitted to pathogenic Neisseria. This study demonstrates the utility of WGS-based surveillance of nonpathogenic Neisseria.

scholarly journals Mosaic structure of the penA gene in the oropharynx of men who have sex with men negative for gonorrhoea

2020 ◽  
Vol 31 (3) ◽  
pp. 230-235 ◽  
Author(s):  
Antonella Marangoni ◽  
Giacomo Marziali ◽  
Melissa Salvo ◽  
Antonietta D’Antuono ◽  
Valeria Gaspari ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Dna Recombination ◽  
Genetic Material ◽  
Mosaic Structure ◽  
Multi Drug Resistance ◽  
Transmitted Infection ◽  
Neisseria Species ◽  
Sexually Transmitted ◽  
Pcr Products ◽  
Sex With Men

The oropharynx represents a crucial site for the emergence of multi-drug resistance in Neisseria gonorrhoeae. The mosaic penA alleles, associated with decreased susceptibility to cephalosporins, have emerged by DNA recombination with partial penA genes, particularly those from commensal pharyngeal Neisseria species. Here, we investigated the prevalence of the mosaic structure of the penA gene in the oropharynx of men who have sex with men testing negative for pharyngeal gonorrhoea. From January 2016 to June 2018, 351 gonorrhoea-negative men who have sex with men attending a sexually transmitted infection clinic in Italy were enrolled. Pharyngeal swabs underwent a real-time polymerase chain reaction (PCR) for the detection of the mosaic penA gene. In case of positivity, PCR products were sequenced and searched against several sequences of Neisseria strains. Overall, 31 patients (8.8%) were found positive for the presence of the mosaic penA gene. The positivity was significantly associated with previous cases of pharyngeal gonorrhoea (relative risk [RR]: 3.56, 95% confidence interval 1.44–8.80) and with recent exposure to beta-lactams (RR: 4.29, 95% confidence interval 2.20–8.38). All penA-positive samples showed a high relatedness (90–99%) with mosaic-positive Neisseria strains. Our data underline that commensal Neisseria species of the oropharynx may be a significant reservoir for genetic material conferring antimicrobial resistance in N. gonorrhoeae.

scholarly journals 1558. Commensal Neisseria Species as a Cause of Disease in Patients Taking Eculizumab

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S484-S485
Author(s):  
Page Crew ◽  
Lucy McNamara ◽  
Peter Waldron ◽  
Lynda McCulley ◽  
S Christopher Jones ◽  
...  
Keyword(s):  
Neisseria Species ◽  
Commensal Neisseria

Is Multilocus Sequence Typing Approach Useful in Identification of Commensal Neisseria from Clinical Samples?

2018 ◽  
Vol 33 (4) ◽  
pp. 248-253
Author(s):  
Arij Mechergui ◽  
Wafa Achour ◽  
Dario Giorgini ◽  
Rekaya Baaboura ◽  
Muhamed-Kheir Taha ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Clinical Samples ◽  
Commensal Neisseria

scholarly journals Commensal Neisseria Are Shared between Sexual Partners: Implications for Gonococcal and Meningococcal Antimicrobial Resistance

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 228 ◽  
Author(s):  
Christophe Van Dijck ◽  
Jolein G. E. Laumen ◽  
Sheeba S. Manoharan-Basil ◽  
Chris Kenyon
Keyword(s):  
Antimicrobial Resistance ◽  
16S Rrna Gene Sequencing ◽  
Sexual Partners ◽  
Rrna Gene ◽  
Intimate Partners ◽  
Dissimilarity Index ◽  
Individual Level ◽  
Sex With Men ◽  
The Individual ◽  
Commensal Neisseria

Antimicrobial resistance in pathogenic Neisseria parallels reduced antimicrobial susceptibility in commensal Neisseria in certain populations, like men who have sex with men (MSM). Although this reduced susceptibility can be a consequence of frequent antimicrobial exposure at the individual level, we hypothesized that commensal Neisseria are transmitted between sexual partners. We used data from a 2014 microbiome study in which saliva and tongue swabs were taken from 21 couples (42 individuals). Samples were analyzed using 16S rRNA gene sequencing. We compared intimate partners with unrelated individuals and found that the oral Neisseria communities of intimate partners were more similar than those of unrelated individuals (average Morisita–Horn dissimilarity index for saliva samples: 0.54 versus 0.71, respectively (p = 0.005); and for tongue swabs: 0.42 versus 0.63, respectively (p = 0.006)). This similarity presumably results from transmission of oral Neisseria through intimate kissing. This finding suggests that intensive gonorrhea screening in MSM may, via increased antimicrobial exposure, promote, rather than prevent, the emergence and spread of antimicrobial resistance in Neisseria. Non-antibiotic strategies such as vaccines and oral antiseptics could prove more sustainable options to reduce gonococcal prevalence.

scholarly journals The Impact of Recent Antibiotic Usage on Oropharyngeal Neisseria spp. in MSM of Hanoi, Vietnam

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S105-S105
Author(s):  
Huan Dong ◽  
Hoa Thi Nguyen ◽  
Minh Binh Xuan Nguyen ◽  
Trung Vu Nguyen ◽  
Folasade May ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Antibiotic Use ◽  
Antibiotic Usage ◽  
The Past ◽  
History Of ◽  
Neisseria Gonorrhea ◽  
Sex With Men ◽  
The Impact ◽  
Susceptibility Status ◽  
Commensal Neisseria

Abstract Background Neisseria gonorrhea (NG) has a long history of gaining resistance to nearly all antimicrobials used for treatment since the 1930s, which makes susceptibility to last-line cephalosporins of dire importance. Horizontal gene transmission is highly prevalent among the Neisseria genus, with genetic fragments from common commensal Neisseria having been found in resistant NG strains. Globally, NG disproportionately affects men-who-have-sex-with-men (MSM) and nearly all ceftriaxone-resistant cases have been reported from pharyngeal samples. Our study aims to assess the impact of antibiotic usage on the antimicrobial susceptibility of oropharyngeal Neisseria spp. Methods MSM from Hanoi, Vietnam, were surveyed regarding antibiotic usage. Pharyngeal swabs were collected and cultured on chocolate and modified-Thayer–Martin agar. Neisseria spp. were subcultured, identified using MALDI-TOF, and antimicrobial susceptibility determined by E-test using NG CLSI MIC breakpoints to ciprofloxacin (CIP), cefixime (CFM), ceftriaxone (CRO), and cefpodoxime (CPD). Results The 225 MSM surveyed were young (mean age 23.9 years, std 4.4). In the past 6 months: 41.6% participants used any antibiotics, among whom 62.4% did not have a prescription; 6.6% used CFM (16.4% more were unsure), among whom 55.6% did not have a prescription; and 4.5% received CRO. From 72 swabs, 102 Neisseria isolates were obtained; N. flavescens (n = 59), N. perflava (n = 14), N. macacae (n = 8), N. sublfava (n = 7), N. gonorrhoeae (n = 5), N. mucosa (n = 3), N. meningitides (n = 3), N. cinerea (n = 2), and N. lactamica (n = 1). 
“Reduced susceptibility” status for any Neisseria spp. was 35.3, 10, 8, and 30% to CIP, CFM, CRO, and CPD, respectively; 61.8% were “resistant” to CIP. MSM who took any antibiotics in the past 6 months were almost twice as likely to have Neisseria spp. with reduced susceptibility to cephalosporins (CFM, CRO, or CPD), RR 1.8 (95% CI 1.1–3.2; P = 0.026) compared with non-antibiotic users. Conclusion Many MSM in Hanoi used antibiotics recently, often without a prescription. Antibiotic use was associated with antimicrobial “reduced susceptibility” of commensal Neisseria spp. to cephalosporins, possibly potentiating resistance acquisition by NG. Disclosures All authors: No reported disclosures.

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