scholarly journals Nasal Inoculation of the Commensal Neisseria lactamica Inhibits Carriage of Neisseria meningitidis by Young Adults: A Controlled Human Infection Study

2015 ◽  
Vol 60 (10) ◽  
pp. 1512-1520 ◽  
Author(s):  
Alice M. Deasy ◽  
Ed Guccione ◽  
Adam P. Dale ◽  
Nick Andrews ◽  
Cariad M. Evans ◽  
...  
Keyword(s):  
Young Adults ◽  
Neisseria Meningitidis ◽  
Human Infection ◽  
Neisseria Lactamica ◽  
Commensal Neisseria ◽  
Infection Study

scholarly journals Immunization with Live Neisseria lactamica Protects Mice against Meningococcal Challenge and Can Elicit Serum Bactericidal Antibodies

2006 ◽  
Vol 74 (11) ◽  
pp. 6348-6355 ◽  
Author(s):  
Yanwen Li ◽  
Qian Zhang ◽  
Megan Winterbotham ◽  
Eva Mowe ◽  
Andrew Gorringe ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Protective Immunity ◽  
Human Infection ◽  
Natural Immunity ◽  
Upper Respiratory Tract ◽  
Nasopharyngeal Colonization ◽  
Neisseria Lactamica ◽  
Upper Respiratory ◽  
Disease Understanding ◽  
Infants And Young Children

ABSTRACT Natural immunity against Neisseria meningitidis is thought to develop following nasopharyngeal colonization with this bacterium or other microbes expressing cross-reactive antigens. Neisseria lactamica is a commensal of the upper respiratory tract which is often carried by infants and young children; epidemiological evidence indicates that colonization with this bacterium can elicit serum bactericidal activity (SBA) against Neisseria meningitidis, the most validated correlate of protective immunity. Here we demonstrate experimentally that immunization of mice with live N. lactamica protects animals against lethal meningococcal challenge and that some, but not all, strains of N. lactamica elicit detectable SBA in immunized animals regardless of the serogroup of N. meningitidis. While it is unlikely that immunization with live N. lactamica will be implemented as a vaccine against meningococcal disease, understanding the basis for the induction of cross-protective immunity and SBA should be valuable in the design of subunit vaccines for the prevention of this important human infection.

Functional diversity of three different DsbA proteins from Neisseria meningitidis

Microbiology ◽  
2004 ◽  
Vol 150 (9) ◽  
pp. 2993-3000 ◽  
Author(s):  
Sunita Sinha ◽  
Paul R. Langford ◽  
J. Simon Kroll
Keyword(s):  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Protein Folding ◽  
Membrane Proteins ◽  
Neisseria Meningitidis ◽  
Outer Membrane Proteins ◽  
Invasive Disease ◽  
Neisseria Lactamica ◽  
Neisseria Subflava ◽  
Neisseria Flavescens

The genome of Neisseria meningitidis serogroup B strain MC58 contains three genes – nmb0278, nmb0294 and nmb0407 – encoding putative homologues of DsbA, a periplasmic thiol disulphide oxidoreductase protein-folding catalyst of the Dsb protein family. DsbA assists the folding of periplasmic and membrane proteins in diverse organisms. While all three cloned genes complemented the DTT sensitivity of dsbA-null Escherichia coli, they showed different activities in folding specific target proteins in this background. NMB0278 protein was the most active in complementing defects in motility and alkaline phosphatase activity, while NMB0294 was the most active in folding periplasmic MalF. NMB0407 showed the weakest activity in all assays. It is extremely unusual for organisms to contain more than one chromosomal dsbA. Among the members of the genus Neisseria, only the meningococcus carries all three of these genes. Strains of Neisseria gonorrhoeae, Neisseria lactamica, Neisseria cinerea and Neisseria polysaccharea contained only homologues of nmb0278 and nmb0407, while Neisseria flava, Neisseria subflava and Neisseria flavescens carried only nmb0294. It is speculated that the versatility of the meningococcus in surviving in different colonizing and invasive disease settings may be derived in part from an enhanced potential to deploy outer-membrane proteins, a consequence of carrying an extended repertoire of protein-folding catalysts.

Comparison of three methods for identification of pathogenic Neisseria species

1979 ◽  
Vol 9 (5) ◽  
pp. 598-600
Author(s):  
P C Appelbaum ◽  
R B Lawrence
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Incubation Period ◽  
Fluorescent Antibody ◽  
Neisseria Species ◽  
Neisseria Lactamica ◽  
Sugar Degradation ◽  
Agar Method ◽  
Neisseria Sicca

A radiometric procedure was compared with the Minitek and Cystine Trypticase Agar sugar degradation methods for identification of 113 Neisseria species (58 Neisseria meningitidis, 51 Neisseria gonorrhoeae, 2 Neisseria lactamica, 2 Neisseria sicca). Identification of meningococci and gonococci was confirmed by agglutination and fluorescent antibody techniques, respectively. The Minitek method identified 97% of meningococci, 92% of gonococci, and 100% of other Neisseria after 4 h of incubation. The radiometric (Bactec) procedure identified 100% of gonococci and 100% of miscellaneous Neisseria after 3 h, but problems were encountered with meningococci: 45% of the later strains yielded index values for fructose between 20 and 28 (recommended negative cut-off point, less than 20), with strongly positive (greater than 100) glucose and maltose and negative o-nitrophenyl-beta-D-galactopyranoside reactions in all 58 strains. The Cystine Trypticase Agar method identified 91% of meningococci, 90% of gonococci, and 100% of other Neisseria after 24 to 48 h. Prolongation of the Cystine Trypticase Agar incubation period led to abnormal lactose/sucrose reactions in some meningococci and gonococci. Radiometric and Minitek systems are more accurate and convenient than Cystine Trypticase Agar techniques, but, on the basis of these results, radiometric fructose sensitivity levels for meningococci need reevaluation.

scholarly journals Pharyngeal carriage of Neisseria meningitidis and Neisseria lactamica in households with infants within areas with high and low incidences of meningococcal disease

1991 ◽  
Vol 106 (3) ◽  
pp. 445-457 ◽  
Author(s):  
S. F. Olsen ◽  
B. Djurhuus ◽  
K. Rasmussen ◽  
H. D. Joensen ◽  
S. O. Larsen ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Odds Ratio ◽  
Lower Risk ◽  
Household Survey ◽  
Faroe Islands ◽  
Odds Ratios ◽  
Neisseria Lactamica ◽  
Group B

SUMMARYIn a household survey in the Faroe Islands, an isolated community with hyperendemic occurrence of meningococcal disease due to serogroup B 15, 1604 persons were examined for pharyngeal carriage of Neisseria meningitidis and N. lactamica. Two areas were chosen having experienced high (HIA), and two having experienced low incidences (LIA) of disease. Living in HIA compared with LIA was associated with higher risk of N. meningitidis B 15 carriage and lower risk of N. lactamica carriage, with odds ratios of 2·7 (95% confidence interval (CI) 1·4–5·1, P = 0·003) and 0·41 (95% CI 0·31–0·53, P < 0·0001), respectively. In HIA the risk of N. meningitidis carriage was much lower in non-carriers than carriers of N. lactamica, with an odds ratio of 0·19 (95% CI 0·08–0·47, P = 0·0003); in LIA this association (odds ratio 0·51, P = 0·05) was much weaker. Children 0–14 years had substantially higher risk of being carriers of N. meningitidis group B 15 if the mothers were so, with an odds ratio of 11 (95% CI 4–29, P < 0·0001).

scholarly journals Comparison of the Inflammatory Responses of Human Meningeal Cells following Challenge with Neisseria lactamica and with Neisseria meningitidis

2006 ◽  
Vol 74 (11) ◽  
pp. 6467-6478 ◽  
Author(s):  
Mark I. Fowler ◽  
Kiave Y. Ho Wang Yin ◽  
Holly E. Humphries ◽  
John E. Heckels ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Meningitidis ◽  
Inflammatory Responses ◽  
Host Cells ◽  
Pathogenic Potential ◽  
Monocyte Chemoattractant Protein 1 ◽  
Gm Csf ◽  
Neisseria Lactamica ◽  
Meningeal Cells ◽  
Macrophage Colony

ABSTRACT The rationale for the present study was to determine how different species of bacteria interact with cells of the human meninges in order to gain information that would have broad relevance to understanding aspects of the innate immune response in the brain. Neisseria lactamica is an occasional cause of meningitis in humans, and in this study we investigated the in vitro interactions between N. lactamica and cells derived from the leptomeninges in comparison with the closely related organism Neisseria meningitidis, a major cause of meningitis worldwide. N. lactamica adhered specifically to meningioma cells, but the levels of adherence were generally lower than those with N. meningitidis. Meningioma cells challenged with N. lactamica and N. meningitidis secreted significant amounts of the proinflammatory cytokine interleukin-6 (IL-6), the C-X-C chemokine IL-8, and the C-C chemokines monocyte chemoattractant protein 1 (MCP-1) and RANTES, but it secreted very low levels of the cytokine growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF). Thus, meningeal cells are involved in the innate host response to Neisseria species that are capable of entering the cerebrospinal fluid. The levels of IL-8 and MCP-1 secretion induced by both bacteria were essentially similar. By contrast, N. lactamica induced significantly lower levels of IL-6 than N. meningitidis. Challenge with the highest concentration of N. lactamica (108 CFU) induced a small but significant down-regulation of RANTES secretion, which was not observed with lower concentrations of bacteria. N. meningitidis (106 to 108 CFU) also down-regulated RANTES secretion, but this effect was significantly greater than that observed with N. lactamica. Although both bacteria were unable to invade meningeal cells directly, host cells remained viable on prolonged challenge with N. lactamica, whereas N. meningitidis induced death; the mechanism was overwhelming necrosis with no significant apoptosis. It is likely that differential expression of modulins between N. lactamica and N. meningitidis contributes to these observed differences in pathogenic potential.

scholarly journals How clonal areNeisseriaspecies? The epidemic clonality model revisited

2015 ◽  
Vol 112 (29) ◽  
pp. 8909-8913 ◽  
Author(s):  
Michel Tibayrenc ◽  
Francisco J. Ayala
Keyword(s):  
Linkage Disequilibrium ◽  
Neisseria Meningitidis ◽  
Genome Sequencing ◽  
Clonal Propagation ◽  
Clonal Evolution ◽  
Whole Genome ◽  
Species Status ◽  
Short Term ◽  
Neisseria Lactamica ◽  
Long Run

The three speciesNeisseria meningitidis,Neisseria gonorrheae, andNeisseria lactamicaare often regarded as highly recombining bacteria.N.meningitidishas been considered a paradigmatic case of the “semiclonal model” or of “epidemic clonality,” demonstrating occasional bouts of clonal propagation in an otherwise recombining species. In this model, occasional clonality generates linkage disequilibrium in the short term. In the long run, however, the effects of clonality are countered by recombination. We show that many data are at odds with this proposal and thatN.meningitidisfits the criteria that we have proposed for predominant clonal evolution (PCE). We point out that (i) the proposed way to distinguish epidemic clonality from PCE may be faulty and (ii) the evidence of deep phylogenies by microarrays and whole-genome sequencing is at odds with the predictions of the semiclonal model. Last, we revisit the species status ofN.meningitidis,N.gonorrheae, andN.lactamicain the light of the PCE model.

scholarly journals Comparative Proteomics Analysis of Two Strains of Neisseria meningitidis Serogroup B and Neisseria lactamica

2015 ◽  
Vol 8 (11) ◽  
Author(s):  
Raheleh Sheikhi ◽  
Mansour Amin ◽  
Maryam Hamidinia ◽  
Mohammad Ali Assarehzadegan ◽  
Soodabeh Rostami ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Comparative Proteomics ◽  
Proteomics Analysis ◽  
Neisseria Lactamica

scholarly journals First case of interconnected clusters of primary meningococcal arthritis and meningococcal meningitis due to Neisseria meningitidis serogroup B in Slovenia

2021 ◽  
pp. 1-9
Author(s):  
Miha Simoniti ◽  
Tanja Selič Kurinčič ◽  
Alenka Trop Skaza ◽  
Ines Kebler ◽  
Metka Paragi ◽  
...  
Keyword(s):  
Young Adults ◽  
Septic Arthritis ◽  
Neisseria Meningitidis ◽  
General Hospital ◽  
Meningococcal Disease ◽  
Meningococcal Meningitis ◽  
Invasive Meningococcal Disease ◽  
First Case ◽  
The Right

In this report, we describe a case of interconnected clusters of invasive meningococcal disease due to Neisseria meningitidis serogroup B:P1.22,14:F5-1(ST-269) in young adults – a boyfriend and a girlfriend. The male was diagnosed with primary meningococcal septic arthritis of the right knee and the female was diagnosed with meningococcal meningitis with meningococcemia a few hours later. Both were hospitalized at the General Hospital Celje and treated with ceftriaxone, but with different outcomes; the female recovered completely, while the male will probably have long-term sequelae of septic arthritis.

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