Urinary Amino Acid and Peptide Excretion Patterns in Patients with Muscular Dystrophy (Duchenne): A Preliminary Study with the AutoAnalyzer

1969 ◽  
Vol 15 (7) ◽  
pp. 545-554 ◽  
Author(s):  
Milton L Maskaleris ◽  
Stanislaw Gross ◽  
Ade T Milhorat
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Muscular Dystrophy ◽  
Urinary Amino Acids ◽  
Male Patients ◽  
Preliminary Study ◽  
Urinary Amino ◽  
First Time ◽  
Peptide Fractions ◽  
Urine Specimens

Abstract An amino acid AutoAnalyzer was used to monitor the excretion patterns of the urinary amino acids and peptides of 7 male patients (aged 3-17) with muscular dystrophy of the Duchenne type. In comparison with the output of 8 healthy boys (aged 3-12), on the average, a reduction in the excretion of Thr, Ser, Gly, Ala, Val, Ile, Tyr, Lys, and His over a 24-hr period was found in the urine of the patients, although the number of analyses was too small for statistically significant conclusions. The content of Asp, Glu, Phe, and Leu was about equal, and Pro and Hyp were present in very small quantities. Also, for the first time, a peptide analyzer was used to study the bound components from urine specimens of the patients. Excretion patterns of several peptides in three of the above patients aged 3-17 were found to differ from those of the controls. Of the six major peptide fractions examined, five were present in smaller amounts in the urine of patients; only one was elevated. It appears from these preliminary results that further study of the excretion patterns of amino acids and peptides of dystrophic patients is worthwhile, and that study of the peptides should be given preference.

scholarly journals Paper Chromatographic Survey of Urinary Amino-acids in Muscular Dystrophy

BMJ ◽  
1970 ◽  
Vol 3 (5713) ◽  
pp. 29-30 ◽  
Author(s):  
L. J. Hurwitz ◽  
D. McCormick
Keyword(s):  
Amino Acids ◽  
Muscular Dystrophy ◽  
Urinary Amino Acids ◽  
Urinary Amino

Urinary amino acids, creatinine and phosphate in muscular dystrophy

1955 ◽  
Vol 54 (2) ◽  
pp. 384-391 ◽  
Author(s):  
Kenneth E. Hurley ◽  
Roger J. Williams
Keyword(s):  
Amino Acids ◽  
Muscular Dystrophy ◽  
Urinary Amino Acids ◽  
Urinary Amino

INFLUENCE OF HUMAN GROWTH HORMONE (HGH) ON PLASMA AND URINE AMINO ACID CONCENTRATIONS IN HYPOPITUITARY DWARFS

1969 ◽  
Vol 62 (3) ◽  
pp. 513-520 ◽  
Author(s):  
M Zachmann
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Amino Acid ◽  
Human Growth Hormone ◽  
Plasma Concentrations ◽  
Human Growth ◽  
Plasma Amino Acids ◽  
Before And After ◽  
Urinary Amino Acids ◽  
Urinary Amino

ABSTRACT Amino acids in the plasma and urine of 6 hypopituitary dwarfs before and after 3–5 days of human growth hormone (HGH) administration (2 mg/m2 daily) were studied. As reported previously, the concentrations of several amino acids in the plasma of untreated hypopituitary dwarfs are low. After administration of HGH certain amino acids in the plasma increased regularly (threonine, serine, glycine, methionine and others) and the overall amino acid pattern in the plasma became normal. Other amino acids did not show consistent changes. The changes in the plasma concentrations do not seem to be related to renal mechanisms, since urinary amino acids and amino acid clearances varied only little. The HGH induced increase of plasma amino acids affects different amino acids than those involved in the increase of plasma amino acids induced by puberty in the male. This suggests that HGH and testosterone have different effects on the amino acid metabolism.

Abstract P037: 24-hour Urinary Amino Acid Excretion And Blood Pressure: The Intermap Study

Circulation ◽  
2014 ◽  
Vol 129 (suppl_1) ◽  
Author(s):  
Martha L Daviglus ◽  
Jeremiah Stamler ◽  
Queenie Chan ◽  
Ian J Brown ◽  
Alan R Dyer ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Body Mass Index ◽  
Amino Acids ◽  
Amino Acid ◽  
Body Mass ◽  
Nitrogen Excretion ◽  
Acid Excretion ◽  
New Findings ◽  
Urinary Amino Acids ◽  
Urinary Amino

Background: Available data indicate inverse relations to blood pressure (BP) of dietary vegetable protein and its main amino acid, glutamic acid, also direct relations to BP of meat and animal protein intakes. Data on association of urinary amino acids (UAAs) with BP are limited. Objective: To assess the relationship between UAAs and BP. Methods: This study assessed relations to BP of 21 urinary amino acids among 4,677 men and women ages 40-59 (17 population samples in China, Japan, UK, USA) from the International Study on Macro/Micronutrients and Blood Pressure (INTERMAP). Urinary amino acid excretion was expressed as log transformed value of each urinary amino acid (μmol/24 h), and as % dietary total protein (based on urinary urea nitrogen excretion). Results: In multivariate analyses with all 21 UAAs considered together, independent inverse relations to BP were found for serine, glycine, and lysine, and independent direct relations to BP for threonine, cystine, and 3-methylhistidine, controlling for possible non-dietary and dietary confounders (including body mass index and each of the other UAAs). For example, with log urinary serine at 75th compared to the 25th percentile, SBP/DBP was lower by 2.4/1.8 mm Hg and 2.2/1.7 mm Hg on average without and with adjustment for body mass index in multivariate models. With log urinary 3-methylhistidine at 75th compared to the 25th percentile, SBP/DBP was higher by 3.8/2.7 and 1.6/1.4 mm Hg. Conclusions: These significant new findings on relations to blood pressure of urinary amino acids need to be verified by additional - including prospective - data.

Sex-Related Patterns in the Profiles of Human Urinary Amino Acids

1975 ◽  
Vol 21 (13) ◽  
pp. 1970-1975 ◽  
Author(s):  
Henri Dirren ◽  
Arthur B Robinson ◽  
Linus Pauling
Keyword(s):  
Amino Acids ◽  
High Pressure ◽  
Amino Acid ◽  
Young Women ◽  
Amino Acid Analyzer ◽  
Amino Acid Profiles ◽  
Urinary Amino Acids ◽  
Urinary Amino ◽  
Nonparametric Statistical ◽  
Related Pattern

Abstract The urinary amino acid profiles of 79 young women and 72 young men were studied with an automated high-pressure amino acid analyzer. A nonparametric statistical analysis was applied for detecting profile differences related to sex and other variables. A strong sex-related pattern was confirmed and its power for sex determination was evaluated. For the women, profile differences related to the use of oral contraceptives were also discovered and evaluated. No correlation with the menstrual cycle was detected.

scholarly journals Profiling at mRNA, protein, and metabolite levels reveals alterations in renal amino acid handling and glutathione metabolism in kidney tissue ofPept2−/−mice

2007 ◽  
Vol 28 (3) ◽  
pp. 301-310 ◽  
Author(s):  
Isabelle M. Frey ◽  
Isabel Rubio-Aliaga ◽  
Anne Siewert ◽  
Daniela Sailer ◽  
Aleksey Drobyshev ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Kidney Tissue ◽  
Urine Samples ◽  
Differentially Expressed ◽  
Glutathione Metabolism ◽  
Altered Expression ◽  
Urinary Amino Acids ◽  
Urinary Amino

PEPT2 is an integral membrane protein in the apical membrane of renal epithelial cells that operates as a rheogenic transporter for di- and tripeptides and structurally related drugs. Its prime role is thought to be the reabsorption of filtered di- and tripeptides contributing to amino acid homeostasis. To elucidate the role of PEPT2 in renal amino acid metabolism we submitted kidney tissues of wild-type and a Pept2−/−mouse line to a comprehensive transcriptome, proteome and metabolome profiling and analyzed urinary amino acids and dipeptides. cDNA microarray analysis identified 147 differentially expressed transcripts in transporter-deficient animals, and proteome analysis by 2D-PAGE and MALDI-TOF-MS identified 37 differentially expressed proteins. Metabolite profiling by GC-MS revealed predominantly altered concentrations of amino acids and derivatives. Urinary excretion of amino acids demonstrated increased glycine and cysteine/cystine concentrations and dipeptides in urine were assessed by amino acid analysis of urine samples before and after in vitro dipeptidase digestion. Dipeptides constituted a noticeable fraction of urinary amino acids in Pept2−/−animals, only, and dipeptide-bound glycine and cystine were selectively increased in Pept2−/−urine samples. These findings were confirmed by a drastically increased excretion of cysteinyl-glycine (cys-gly). Urinary loss of cys-gly together with lower concentrations of cysteine, glycine, and oxoproline in kidney tissue and altered expression of mRNA and proteins involved in glutathione (GSH) metabolism suggests that PEPT2 is predominantly a system for reabsorption of cys-gly originating from GSH break-down, thus contributing to resynthesis of GSH.

Single Amino Acid Based Self-Assemblies of Cysteine and Methionine

2018 ◽  
Author(s):  
Nidhi Gour ◽  
Bharti Koshti ◽  
Chandra Kanth P. ◽  
Dhruvi Shah ◽  
Vivek Shinh Kshatriya ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Self Assembly ◽  
Aromatic Amino Acids ◽  
Neutral Condition ◽  
Single Amino Acid ◽  
Binding Assays ◽  
Human Neuroblastoma ◽  
Cytotoxicity Assays ◽  
First Time

We report for the very first time self-assembly of Cysteine and Methionine to discrenible strucutres under neutral condition. To get insights into the structure formation, thioflavin T and Congo red binding assays were done which revealed that aggregates may not have amyloid like characteristics. The nature of interactions which lead to such self-assemblies was purported by coincubating assemblies in urea and mercaptoethanol. Further interaction of aggregates with short amyloidogenic dipeptide diphenylalanine (FF) was assessed. While cysteine aggregates completely disrupted FF fibres, methionine albeit triggered fibrillation. The cytotoxicity assays of cysteine and methionine structures were performed on Human Neuroblastoma IMR-32 cells which suggested that aggregates are not cytotoxic in nature and thus, may not have amyloid like etiology. The results presented in the manuscript are striking, since to the best of our knowledge,this is the first report which demonstrates that even non-aromatic amino acids (cysteine and methionine) can undergo spontaneous self-assembly to form ordered aggregates.

scholarly journals Amino Acid and Acylcarnitine Levels in Chronic Patients with Schizophrenia: A Preliminary Study

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 34
Author(s):  
Irina A. Mednova ◽  
Alexander A. Chernonosov ◽  
Marat F. Kasakin ◽  
Elena G. Kornetova ◽  
Arkadiy V. Semke ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Amino Acid ◽  
Chronic Schizophrenia ◽  
Integrated Approach ◽  
Serum Levels ◽  
Tandem Mass ◽  
Chronic Patients ◽  
Healthy Donors ◽  
Preliminary Study

Amino acids and acylcarnitines play an important role as substrates and intermediate products in most of pathways involved in schizophrenia development such as mitochondrial dysfunction, inflammation, lipid oxidation, DNA damage, oxidative stress, and apoptosis. It seems relevant to use an integrated approach with ‘omics’ technology to study their contribution. The aim of our study was to investigate serum amino acid and acylcarnitine levels in antipsychotics-treated patients with chronic schizophrenia compared with healthy donors. We measured serum levels of 15 amino acids and 30 acylcarnitines in 37 patients with schizophrenia and 36 healthy donors by means of tandem mass spectrometry. In summary, patients with chronic schizophrenia had an altered concentration of a few amino acids and acylcarnitines in comparison to the healthy probands. Further research is needed to assess and understand the identified changes.

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