HPLC analysis of brain and plasma for octanoic and decanoic acids.

1989 ◽  
Vol 35 (9) ◽  
pp. 1945-1948 ◽  
Author(s):  
H G Dean ◽  
J C Bonser ◽  
J P Gent
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
High Performance ◽  
Medium Chain Triglyceride ◽  
Brain Homogenate ◽  
Gas Liquid Chromatography ◽  
Medium Chain ◽  
Detection Analysis ◽  
Coefficients Of Variation

Abstract Two methods are described for determination of octanoic and decanoic acids in plasma and brain homogenate by "high-performance" liquid chromatography with ultraviolet detection. Analysis of the underivatized acids had a detection limit of only 50 mg/L, but formation of the p-bromophenacyl ester increased the sensitivity by 100-fold, to a detection limit of 0.5 mg/L. The latter procedure gave interassay coefficients of variation of 4.1% and 4.8% for octanoic and decanoic acids, respectively. The corresponding intra-assay values were 3.95% and 4.7% (n = 6). The derivative method, applied to samples of plasma from children receiving a medium-chain triglyceride (MCT) diet, gave values in agreement with results by gas-liquid chromatography. Results have also been obtained for samples from mice, either treated with the medium-chain triglyceride diet or given infusions of sodium octanoate.

scholarly journals Validation of High Performance Liquid Chromatography Methods for Determination of Meloxicam and Tenoxicam from Transdermal Therapeutic Systems

2017 ◽  
Vol 63 (4) ◽  
pp. 178-182 ◽  
Author(s):  
Paula Antonoaea ◽  
Anca Gabriela Cârje ◽  
Adriana Ciurba ◽  
Nicoleta Todoran ◽  
Alexandru Robert Vlad ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
High Performance ◽  
Hydroxypropyl Methylcellulose ◽  
Active Pharmaceutical Ingredient ◽  
Performance Criteria ◽  
Quantification Limit ◽  
Transdermal Patches ◽  
Evaporation Technique

AbstractObjective: The aim of this study was to develop and validate two HPLC methods for the quantification of meloxicam and tenoxicam from transdermal therapeutic systems.Methods: Based on 1.0% hydroxypropyl methylcellulose 15000, transdermal patches containing meloxicam or tenoxicam were prepared by solvent evaporation technique. Analytical performances of the HPLC methods for the quantification of meloxicam and tenoxicam from such systems were assessed in terms of specificity, linearity, detection limit, quantification limit, recovery and precision.Results and discussion: The linearity of the method was assessed through a calibration curve in the 1.0 - 75.0 μg∙mL−1concentration range, with a regression coefficient higher than 0.999. The detection limit and the quantification limit were found to be 0.46 μg∙mL−1and 1.39 μg∙mL−1, for meloxicam; and 0.88 μg∙mL−1, respectively 2.64 μg∙mL−1for tenoxicam. According to the European Pharmacopeia 5.0 the mean recovery was found to be between 75% and 125%. As performance criteria for precision was used the RSD% which were lower than 2.0% for both methods.Conclusions: The proposed liquid chromatography methods provide selective, linear and precise results for the quantification of meloxicam and tenoxicam from transdermal therapeutic systems. The presence of a single peak in the chromatograms of the analyzed transdermal patches with meloxicam or tenoxicam, certify the successful determination of the active pharmaceutical ingredient in the prepared patches.

Determination of Sugars in β-Amylase Hydrolysates by High Performance Liquid Chromatography

2011 ◽  
Vol 396-398 ◽  
pp. 1575-1578
Author(s):  
Xin Hong Liang ◽  
Bin Li ◽  
Gang Li ◽  
Yu Tang ◽  
Zu Feng Guo
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Detection Limit ◽  
Correlation Coefficient ◽  
Trace Amount ◽  
High Performance ◽  
Enzymes Activity ◽  
Differential Refractometer

Abstract. The chromatographic conditions for the separation of glucose, maltose and maltotriose on a ZORBAX Eclipse XDB-C18 column (4 um, 4.6×250mm) by use of a 2414 differential refractometer detector were determined. The square of the correlation coefficient of glucose, sucrose, maltose and maltotriose was 0.9973, 0.9981, 0.9977 and 0.9987, respectively. When the ratio of signal and noise was 3, the detection limit of glucose, sucrose, maltose and maltotriose was 0.3, 0.3, 0.20, 0.20 mg/mL the β-amylase hydrolysates consisted mainly of maltose, and maltotriose next, glucose only in trace amount, and no sucrose. The RSD of glucose, maltose and maltotriose was 2.08%, 2.41% and 2.61%, respectively. And the recovery of glucose, maltose and maltotriose was 101.3%, 98.4% and 93.5%. It was credible to separate sugars from hydrolysates. The method is important for improving the inducible enzymes activity.

Liquid chromatography of codeine in plasma with fluorescence detection.

1982 ◽  
Vol 28 (5) ◽  
pp. 1137-1139 ◽  
Author(s):  
I W Tsina ◽  
M Fass ◽  
J A Debban ◽  
S B Matin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Time Profile ◽  
Concentration Time ◽  
Coefficients Of Variation ◽  
Natural Fluorescence ◽  
Routine Assay

Abstract This analytical method for easier determination of codeine in human plasma is based on "high-performance" liquid chromatography for separation and the natural fluorescence of codeine for detection. Codeine is extracted from alkalinized plasma with a mixture of hexane and dichloromethane, and the extract is further purified and chromatographed. The method can be used for routine assay of codeine at the concentrations of 10 micrograms/L or greater in human plasma. As little as 4 micrograms/L can be detected. Coefficients of variation for the assay of codeine in the concentration range of 10 to 100 micrograms/L were 2.2-7.4% (n = 6). We used this method to establish a concentration/time profile for plasma from a human volunteer after a 60-mg oral dose of codeine sulfate.

Determination of Amitraz in Cattle Dipping Baths and Sprays

1978 ◽  
Vol 61 (6) ◽  
pp. 1516-1518
Author(s):  
Michael V Machin ◽  
Kerryn W Mcdougall
Keyword(s):  
Liquid Chromatography ◽  
Petroleum Ether ◽  
Coefficient Of Variation ◽  
Gas Liquid Chromatography ◽  
Chromatographic Methods ◽  
Coefficients Of Variation

Abstract Infrared (IR) and gas chromatographic methods have been developed for determining the acaricide amitraz (N-(2,4-dimethylphenyl)-N - [[(2,4- dimethylphenyl)imino]methyl] - N - methanimidamide) in cattle dip solutions. Extraction with petroleum ether, followed by IR and gas-liquid chromatography (GLC) gave coefficients of variation of 3.4 and 3.6%, respectively, for 0.010-0.035% amitraz. Dilution of the dip sample with acetone and determination by GLC gave a coefficient of variation of 3.6% in the same range. Recovery of amitraz from fortified dip samples was 97–100%.

scholarly journals Residue Analysis for Halofuginone in Sturgeon Muscle by Immunoaffinity Cleanup and Liquid Chromatography

2005 ◽  
Vol 88 (6) ◽  
pp. 1644-1648 ◽  
Author(s):  
Shuangyang Ding ◽  
Yali Hou ◽  
Ningpeng Wu ◽  
Jiangzhong Shen
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Cyanogen Bromide ◽  
Polyclonal Antibodies ◽  
Residue Analysis ◽  
Immunoaffinity Chromatography ◽  
Absorbance Detection ◽  
Coefficients Of Variation ◽  
Sepharose 4B

Abstract A high-performance liquid chromatography (LC) method was developed for the determination of halofuginone (HFG) in sturgeon muscle. The extracted samples were cleaned up by an immunoaffinity chromatography column that was prepared by covalently coupling polyclonal antibodies against HFG to cyanogen bromide (CNBr) activated Sepharose 4B. The eluate was evaporated to dryness, and residues were determined by LC with absorbance detection at 243 nm. Recoveries of HFG from samples fortified at 20–200 μg/kg levels ranged 74.6–81.1%, with coefficients of variation of 0.7–8.6%. The detection limit was estimated to be 10 μg/kg in a 2 g sample.

High-Speed Liquid Chromatographic Cleanup of Environmental Samples Prior to the Gas Chromatographic Determination of Lindane

1974 ◽  
Vol 57 (5) ◽  
pp. 1046-1049
Author(s):  
Richard H Larose
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Environmental Samples ◽  
High Speed ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatograph ◽  
Cleanup Procedure ◽  
Liquid Chromatographic

Abstract A method is described which permits the removal of interfering co-extractives from lindane, using high-speed liquid chromatography. Fractions are collected from the liquid chromatograph for further analysis by gas-liquid chromatography. The cleanup procedure takes less than 5 min and recoveries of more than 90% are obtained. The detection limit for water samples is 5 ng/L.

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