1173Anti-heart and anti-intercalated disk autoantibodies: possible novel biomarkers of cardiac sarcoidosis

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
A Caforio ◽  
S Gianstefani ◽  
A Baritussio ◽  
R Marcolongo ◽  
M Seguso ◽  
...  
Keyword(s):  
Potential Role ◽  
Cardiac Involvement ◽  
Cardiac Sarcoidosis ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Non Invasive ◽  
Immune Mediated ◽  
Novel Biomarkers ◽  
Intercalated Disk

Abstract Background Sarcoidosis is an immune-mediated disease; cardiac involvement, a granulomatous form of myocarditis, is under-recognised and prognostically relevant, as it can present with significant morbidity and mortality. Anti-heart autoantibodies (AHA) and anti-intercalated disk autoantibodies (AIDA) are reliable autoimmune markers in non-sarcoidosis myocarditis forms. Purpose The aim of this study was to assess the potential role of serum AHA and AIDA in cardiac sarcoidosis. Methods This is a cross-sectional study on a series of 29 patients with biopsy proven extra-cardiac sarcoidosis and with biopsy-proven or clinically suspected cardiac involvement, who were tested for AHA and AIDA. Patients were recruited in two recruiting tertiary centres, in USA and Italy. AHA and AIDA were detected by indirect immunofluorescence on human myocardium and skeletal muscle. Controls included sera from patients with non-inflammatory cardiac disease (NICD) (n=160), with ischemic heart failure (IHF) (n=141) and normal blood donors (NBD) (n=270). Results The frequencies of AHA and of AIDA were higher in sarcoidosis (86%; 62%) than in NICD (8%; 4%), IHF (7%; 2%), NBD (9%; 0%) (p=0.0001; p=0.0001 respectively). Sensitivity and specificity were: 86% and 92% for positive AHA and 62% and 98% for positive AIDA, respectively (see figure). Figure 1 Conclusions The detection of serum AHA and AIDA in biopsy-proven or clinically suspected cardiac sarcoidosis supports the involvement of heart-specific autoimmunity in the majority of our cases and may provide a novel non invasive diagnostic marker.

scholarly journals Serum Anti-Heart and Anti-Intercalated Disk Autoantibodies: Novel Autoimmune Markers in Cardiac Sarcoidosis

2021 ◽  
Vol 10 (11) ◽  
pp. 2476
Author(s):  
Alida L. P. Caforio ◽  
Anna Baritussio ◽  
Renzo Marcolongo ◽  
Chun-Yan Cheng ◽  
Elena Pontara ◽  
...  
Keyword(s):  
Cardiac Involvement ◽  
Cardiac Sarcoidosis ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Cardiac Symptoms ◽  
Positron Emission ◽  
Immune Mediated ◽  
The Usa ◽  
And Control ◽  
Intercalated Disk

Background: Sarcoidosis is an immune-mediated disease. Cardiac involvement, a granulomatous form of myocarditis, is under-recognized and prognostically relevant. Anti-heart autoantibodies (AHAs) and anti-intercalated disk autoantibodies (AIDAs) are autoimmune markers in nonsarcoidosis myocarditis forms. Objective: The aim was to assess serum AHAs and AIDAs as autoimmune markers in cardiac sarcoidosis. Methods: This is a cross-sectional study on AHA and AIDA frequency in: 29 patients (aged 46 ± 12, 20 male) with biopsy-proven extracardiac sarcoidosis and biopsy-proven or clinically suspected and confirmed by 18-fluorodeoxyglucose positron emission tomography and/or cardiovascular magnetic resonance (CMR) cardiac involvement; 30 patients (aged 44 ± 11, 12 male) with biopsy-proven extracardiac sarcoidosis without cardiac involvement (no cardiac symptoms, normal 12-lead electrocardiogram, echocardiography and CMR), and control patients with noninflammatory cardiac disease (NICD) (n = 160), ischemic heart failure (IHF) (n = 141) and normal blood donors (NBDs) (n = 270). Sarcoidosis patients were recruited in two recruiting tertiary centers in the USA and Italy. AHAs and AIDAs were detected by indirect immunofluorescence on the human myocardium and skeletal muscle. Results: AHA and AIDA frequencies were higher in sarcoidosis with cardiac involvement (86%; 62%) than in sarcoidosis without cardiac involvement (0%; 0%), NICD (8%; 4%), IHF (7%; 2%) and NBD (9%; 0%) (p = 0.0001; p = 0.0001, respectively). Sensitivity and specificity for cardiac sarcoidosis were 86% and 92% for positive AHAs and 62% and 98% for positive AIDAs, respectively. AIDAs in cardiac sarcoidosis were associated with a higher number of involved organs (p = 0.04). Conclusions: Serum AHAs and AIDAs provide novel noninvasive diagnostic autoimmune markers for cardiac sarcoidosis.

scholarly journals Urine Phenylacetylglutamine Determination in Patients with Hyperphenylalaninemia

2021 ◽  
Vol 10 (16) ◽  
pp. 3674
Author(s):  
Fernando Andrade ◽  
Ainara Cano ◽  
María Unceta Suarez ◽  
Arantza Arza ◽  
Ana Vinuesa ◽  
...  
Keyword(s):  
Potential Role ◽  
Venous Blood ◽  
Phenyl Group ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Non Invasive ◽  
Frequent Monitoring ◽  
Healthy Control

Phenylketonuria (PKU), an autosomal-recessive inborn error of phenylalanine (Phe) metabolism is the most prevalent disorder of amino acid metabolism. Currently, clinical follow-up relies on frequent monitoring of Phe levels in blood. We hypothesize that the urine level of phenylacetylglutamine (PAG), a phenyl-group marker, could be used as a non-invasive biomarker. In this cross-sectional study, a validated liquid chromatography coupled to tandem mass spectrometry (LC-MS) method was used for urinary PAG quantification in 35 participants with hyperphenylalaninemia (HPA) and 33 age- and sex-matched healthy controls. We have found that (a) PKU patients present higher urine PAG levels than healthy control subjects, and that (b) there is a significant correlation between urine PAG and circulating Phe levels in patients with HPA. In addition, we show a significant strong correlation between Phe levels from venous blood samples and from capillary finger-prick dried blood spot (DBS) samples collected at the same time in patients with HPA. Further research in order to assess the potential role of urine PAG as a non-invasive biomarker in PKU is warranted.

scholarly journals Insights into the Predictors of Attitude toward Entomophagy: The Potential Role of Health Literacy: A Cross-Sectional Study Conducted in a Sample of Students of the University of Florence

2021 ◽  
Vol 18 (10) ◽  
pp. 5306
Author(s):  
Chiara Lorini ◽  
Laura Ricotta ◽  
Virginia Vettori ◽  
Marco Del Riccio ◽  
Massimiliano Alberto Biamonte ◽  
...  
Keyword(s):  
Data Analysis ◽  
Health Literacy ◽  
Potential Role ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
University Of Florence ◽  
The University ◽  
Self Administered Questionnaire

In Western countries, one of the main barriers to entomophagy is repulsion toward insects. Few studies have investigated the factors that influence attitudes toward entomophagy. Therefore, we conducted a cross-sectional study involving a sample of 248 university students, focusing on disgust and other potential attributes that can influence insect consumption, including health literacy. We used a 17-item self-administered questionnaire. Consistent with the literature, two items were chosen as outcome variables to evaluate the predictors of the propensity to consume insects: “Have you ever eaten insects or insect-based products?” and “How disgusting do you find eating insects?” The data analysis shows that having already eaten insects is inversely associated with the level of disgust (OR: 0.1, p < 0.01); and it is positively associated with higher levels of health literacy (OR: 3.66, p > 0.01). Additionally, having some knowledge and information about entomophagy is inversely associated with a higher level of disgust (OR: 0.44, p = 0.03 and OR: 0.25, p = 0.03, respectively), while being female is positively associated with disgust (OR: 3.26, p < 0.01). Our results suggest the potential role of health literacy, in addition to other factors, in influencing the willingness to taste insects. However, further studies involving larger and non-convenience samples are needed to confirm our hypothesis.

Risk factors associated with sensitive skin and potential role of lifestyle habits: a cross-sectional study

2017 ◽  
Vol 42 (6) ◽  
pp. 656-658 ◽  
Author(s):  
D. Falcone ◽  
R. J. H. Richters ◽  
N. E. Uzunbajakava ◽  
P. E. J. van Erp ◽  
P. C. M. van de Kerkhof
Keyword(s):  
Risk Factors ◽  
Potential Role ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Sensitive Skin ◽  
Lifestyle Habits ◽  
Cross Sectional ◽  
Factors Associated

scholarly journals Papel potencial do enfermeiro no enfrentamento do problema da tuberculose junto ao Agente Comunitário de Saúde no Programa de Controle da Tuberculose

2013 ◽  
Vol 4 (2) ◽  
pp. 106
Author(s):  
Alaine Maria Da Costa ◽  
Gislane De Sousa Rodrigues ◽  
Tatiana Maria De Melo Guimarães Dos Santos
Keyword(s):  
Community Health ◽  
Potential Role ◽  
Family Health ◽  
Cross Sectional Study ◽  
Tuberculosis Control ◽  
Cross Sectional ◽  
Health Strategy ◽  
Family Health Strategy ◽  
Community Health Agent

Resumo: Objetivou-se refletir e discutir sobre o papel potencial do enfermeiro no enfrentamento do problema da tuberculose junto ao Agente Comunitário de Saúde no Programa de Controle da Tuberculose. Trata-se de pesquisa descritiva, de abordagem quantitativa, com desenho de estudo transversal. A amostra constituiu-se de 121 Agentes Comunitários de Saúde (ACS) da Estratégia Saúde da Família (ESF) - zona urbana de Teresina. Evidenciou-se que 71,1% dos sujeitos admitiram procurar o enfermeiro da ESF em caso de dúvidas frente a casos suspeitos de tuberculose. Ao enfermeiro cabem intervenções pertinentes no controle da tuberculose tanto no âmbito político quanto operativo.Palavras-Chave: Enfermagem, Atenção Básica à Saúde, Tuberculose.The potential role of nurses in addressing the problem of tuberculosis with the Community Health Agent Program Control TuberculosisAbstract: The objective of this study was to reflect and discuss about the potential role of the nurses at the tuberculosis combat, helping the Community Health Agent at the Program of Tuberculosis Control. This is a descriptive study that uses a quantitative approach, with a cross-sectional study design. The group studied was formed of 121 Community Health Agents of The Family Health Strategy in the urban zone of Teresina. It was observed that 71.1% of the agents admitted to need help of a nurse when they have doubts related to suspected cases of tuberculosis. Relevant interventions in tuberculosis control are responsibility of the nurses at a political and an operational scope.Keywords: Nursing, Basic Attention Health, Tuberculosis.El papel potencial de las enfermeras para resolver el problema de la tuberculosis con el control de la Salud de la Comunidad del Agente de Program Tuberculosis.Resumen: Se objetivó con este estudio reflexionar y debatir sobre el papel potencial de los enfermeros en la lucha contra la tuberculosis, junto a los agentes comunitarios de salud en el Programa de Control de la Tuberculosis. Este es un estudio descriptivo que utiliza un abordaje cuantitativo, con un diseño de estudio transversal. El grupo estudiado poseía 121 ACS de la Estrategia Salud de la Familia - zona urbana de Teresina. Se observó que 71,1% de los agentes admiten buscar a un enfermero cuando existen dudas en relación a los casos sospechosos de tuberculosis. Al enfermero, caben las intervenciones pertinentes en el control de la tuberculosis tanto en el alcance político como operacionalPalabras Clave: Enfermería, Atención Básica a la Salud, Tuberculosis.

scholarly journals Role of Growth factors (HB-EGF, VEGF- A) and Immunotolerance Mediators (TNF-alphaIFN-gamma,PD-L1 and IL-10) in Acute and Chronic Otitis Media

2020 ◽  
Author(s):  
SELVAKUMARI MATHIYALAGAN ◽  
Anand D Karthikeyan ◽  
Ramakrishnan R ◽  
Melvin George
Keyword(s):  
Otitis Media ◽  
Chronic Otitis Media ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Luminex Assay ◽  
Tympanic Membrane Perforations ◽  
Immune Mediated ◽  
Disease Condition ◽  
Severity Of The Disease

Abstract Objectives: There is an increasing evidence of immune mediated mechanism in the etiopathogenesis of Otitis Media prime middle ear inflammation prime. The aim of the present study was to determine the expression of important circulatory regulators of immunotolerance as biomarkers. Materials and Methods In this cross sectional study, a total of 44 OM patients and 37 controls were included. Blood plasma level of HB-EGF, IL-10, TNF-alpha, IFN-gamma, PD-L1 and VEGF-A were quantified using Human Magnetic Luminex assay. Results The study showed statistical significant differences in the levels of VEGF-A between OM patients with and without tympanic membrane perforations (p<0.05). Moreover, we found comparatively higher level of PD-L1 in OM patients than controls. However, the level of growth factor HB-EGF, is significantly higher in controls than the cases (p<0.05). There was also a correlation between the levels of HB-EGF and VEGF-A and the severity of the disease condition. Conclusions Role of inflammatory mediators and cytokines like PD-L1, IFNgamma, TNFalpha and IL-10in OM patients as a biomarkers are very minimal. VEFG-A have a significant role in the pathogenesis of OM.Further studies are required for better understanding of the role of these immunosuppressive mediators in the eitopathogenesis of OM.

scholarly journals Role of Ultrasound in Determining the Nature of Pleural Effusion

2014 ◽  
Vol 4 (1) ◽  
pp. 34-37
Author(s):  
N Agarwal ◽  
HM Kansal ◽  
SK Bhargava
Keyword(s):  
Pleural Effusion ◽  
Cross Sectional Study ◽  
High Frequency Ultrasound ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Pleural Thickening ◽  
Non Invasive ◽  
One Year ◽  
Frequency Ultrasound

Purpose of the study: The differentiation of pleural effusion into transudate and exudate may affect the diagnostic approach and patients’ management. The conventional Light criteria require both pleural fluid and blood samples along with three biochemical tests. This study was undertaken to assess the usefulness of ultrasound as an alternative in differentiating the type of pleural effusion. Method: A prospective cross sectional study was conducted over a period of one year. Eighty patients clinically diagnosed to have pleural effusion underwent high frequency ultrasound and also lab tests for Light criteria. The efficacy of ultrasound was then assessed in differentiating transudate from exudate. Results: It was concluded in our study that all pleural effusions which were echogenic on ultrasound, with or without septations or with pleural thickening more than 3 mm (n=46) were exudates (p< 0.01). The anechoic effusions on ultrasound could be transudates or exudates. Conclusion: Ultrasound acts as a useful non invasive alternative for determining the type of pleural effusion. DOI: http://dx.doi.org/10.3126/njr.v4i1.11367 Nepalese Journal of Radiology, Vol.4(1) 2014: 34-37

scholarly journals Pancreatitis kills cysts: A phenomenon that illustrates the potential role of immune activation in premalignant cyst ablation

2020 ◽  
Vol 08 (08) ◽  
pp. E1039-E1041
Author(s):  
Leonard T. Walsh ◽  
Matthew T. Moyer
Keyword(s):  
Potential Role ◽  
Cystic Lesions ◽  
Cross Sectional ◽  
Case Presentation ◽  
Limited Efficacy ◽  
Immune Mediated ◽  
Tumor Killing ◽  
Cross Sectional Imaging ◽  
Sectional Imaging

Abstract Background and study aims Pancreatic cystic lesions are being increasingly recognized on cross-sectional imaging and mucinous premalignant cysts represent one of the most prevalent types. Endoscopic ultrasound (EUS)-guided chemoablation offers a safe and minimally invasive ablation approach yet with limited efficacy. Patients who develop acute pancreatitis as a complication of chemoablation with alcohol however experience ablation rates up to 100 %. This evidence suggests that recruitment of the body’s immune system may represent an avenue to significantly increase the efficacy of EUS-guided chemoablation. Here we illustrate this immune-mediated tumor killing phenomenon through a case presentation at our institution.

scholarly journals Noninvasive Evaluation of Portal Hypertension: Emerging Tools and Techniques

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
V. K. Snowdon ◽  
N. Guha ◽  
J. A. Fallowfield
Keyword(s):  
Portal Hypertension ◽  
Endothelial Dysfunction ◽  
Vascular Injury ◽  
Potential Role ◽  
Noninvasive Evaluation ◽  
Non Invasive ◽  
Novel Biomarkers ◽  
Noninvasive Markers ◽  
Tools And Techniques

Portal hypertension is the main cause of complications in patients with cirrhosis. However, evaluating the development and progression of portal hypertension represents a challenge for clinicians. There has been considerable focus on the potential role of noninvasive markers of portal hypertension that could be used to stratify patients with respect to the stage of portal hypertension and to monitor disease progression or treatment response in a longitudinal manner without having to undertake repeated invasive assessment. The pathogenesis of portal hypertension is increasingly understood and emerging knowledge of the vascular processes that underpin portal hypertension has paved the way for exploring novel biomarkers of vascular injury, angiogenesis, and endothelial dysfunction. In this paper we focus on the pathogenesis of portal hypertension and potential non-invasive biomarkers with particular emphasis on serum analytes.

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