scholarly journals Microbial retention and resistances in stormwater quality improvement devices treating road runoff

FEMS Microbes ◽  
2021 ◽  
Author(s):  
Renato Liguori ◽  
Steffen H Rommel ◽  
Johan Bengtsson-Palme ◽  
Brigitte Helmreich ◽  
Christian Wurzbacher
Keyword(s):  
Heavy Metal ◽  
Quality Improvement ◽  
Microbial Community ◽  
Microbial Communities ◽  
Resistance Genes ◽  
Current Knowledge ◽  
Hot Spot ◽  
Road Runoff ◽  
Stormwater Quality ◽  
Dissolved Heavy Metals

Abstract Current knowledge about the microbial communities that occur in in urban road runoff is scarce. Road runoff of trafficked roads can be heavily polluted and is treated by stormwater quality improvement devices (SQIDs). However, microbes may influence the treatment process of these devices or could lead to stress resistant opportunistic microbial strains. In this study, the microbial community in the influent, effluent and the filter materials for the removal of dissolved heavy metals of two different SQIDs were analyzed to determine the microbial load, retention, composition, and mobile resistance genes. Although the microbes were replaced by new taxa in the effluent, there was no major retention of microbial genera. Further, the bacterial abundance of the SQIDs effluent was relatively stable over time. The heavy metal content correlated with intl1 and with microbial genera. The filter media itself was enriched with Intl1 gene cassettes, carrying several heavy metal and multidrug resistance genes (e.g. czrA, czcA, silP, mexW and mexI), indicating that this is a hot spot for horizontal gene transfer. Overall, the results shed light on road runoff microbial communities, and pointed to distinct bacterial communities within the SQIDs, which subsequently influence the microbial community and the genes released with the treated water.

scholarly journals Microbial retention and resistances in stormwater quality improvement devices treating road runoff

2021 ◽  
Author(s):  
Renato Liguori ◽  
Steffen H. Rommel ◽  
Johan Bengtsson-Palme ◽  
Brigitte Helmreich ◽  
Christian Wurzbacher
Keyword(s):  
Heavy Metal ◽  
Quality Improvement ◽  
Microbial Community ◽  
Resistance Genes ◽  
Current Knowledge ◽  
Bioactive Compound ◽  
Road Runoff ◽  
Stormwater Quality ◽  
Filter Media ◽  
Microbial Composition

AbstractCurrent knowledge about the microbial communities inhabiting the stormwater quality improvement devices (SQIDs) for road runoff is scarce. However, as a bioactive compound of these systems, microbes can facilitate water quality improvement through the biodegradation or precipitation of dissolved contaminants. On the other hand, these contaminants may select for stress resistant opportunistic microbial strains, which are discharged into surface waters or groundwater. In this study, the microbial community of two SQIDs with different design were analyzed to determine the microbial load, retention, composition, and mobile resistance genes in the filter media and the microbial composition in the treated runoff. The bacterial abundance of the SQIDs was relatively stable over time in effluent water samples. Although the microbes were replaced by new taxa in the effluent, there was no major retention of cells or microbial genera. The communities were influenced both by seasonality and by the SQID design. The heavy metal content of the SQIDs was correlated to intl1 and distinct microbial groups. The filter media led to an enrichment and subsequent discharge of Intl1 gene cassettes carrying several heavy metal and multidrug resistance genes (e.g. czrA, czcA, silP, mexW and mexI). Overall, the results suggest that different engineering designs affect the bacterial communities of the SQIDs, and subsequently influence the microbial community and the genes released with the treated water.

scholarly journals Exploring metal resistance genes and mechanisms in copper enriched metal ore metagenome

2020 ◽  
Author(s):  
Esmaeil Forouzan ◽  
Ali Asghar Karkhane ◽  
Bagher Yakhchali
Keyword(s):  
Heavy Metal ◽  
Microbial Community ◽  
Microbial Communities ◽  
Resistance Genes ◽  
Contaminated Soils ◽  
Phylogenetic Profile ◽  
Metal Resistance ◽  
Copper Resistance ◽  
Composition Analysis ◽  
Significant Difference

AbstractHeavy metal pollution is a major global health challenge. In order to develop bioremediation solution for decontamination of environment from heavy metals one appropriate step is to investigate heavy metal resistance strategies used by microbial communities in the metal contaminated environments. The aim of the present study was to understand detailed mechanisms by which long time heavy metal (HM) exposed microbial community use to cope with excess of HMs. We exploited the Illumina high throughput metagenomic approach to examine taxonomical and functional diversity of copper enriched soil metagenome. Three enriched metagenomes were compared against 94 metagenomes derived from non-contaminated soils. Taxonomic composition analysis showed that phylogenetic profile of metal contaminated soils were enriched with γ-Proteobacteria. Comparison of functional profile of the two group reveled significant difference with potential role in HM resistance (HMR). Enriched SEED categories were “Membrane Transport”, “Cell Wall and Capsule”, “Stress Response”, “Iron acquisition and metabolism” and “virulence and defense mechanisms”. Raw metagenomic reads were assembled into scaffolds and predicted Open Reading Frames (ORFs) were searched against metal resistance gene database (BacMet). Based on enriched genes and gene categories and search of known HMR genes we concluded the microbial community cope with HM using at least 10 different mechanisms. Copper resistance genes were more abundant in the metagenome relative to other metals and pumping metals out of the cell were more abundant relative to other HMR mechanism. Results of the present study could be very helpful in understanding of HMR mechanism used by microbial communities.

scholarly journals Microbial Community Stratification Linked to Utilization of Carbohydrates and Phosphorus Limitation in a Boreal Peatland at Marcell Experimental Forest, Minnesota, USA

2014 ◽  
Vol 80 (11) ◽  
pp. 3518-3530 ◽  
Author(s):  
Xueju Lin ◽  
Malak M. Tfaily ◽  
J. Megan Steinweg ◽  
Patrick Chanton ◽  
Kaitlin Esson ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Community Composition ◽  
Hot Spot ◽  
Microbial Community Composition ◽  
Phosphorus Limitation ◽  
Rrna Gene ◽  
Content Type ◽  
Gene Abundance ◽  
P Limitation

ABSTRACTThis study investigated the abundance, distribution, and composition of microbial communities at the watershed scale in a boreal peatland within the Marcell Experimental Forest (MEF), Minnesota, USA. Through a close coupling of next-generation sequencing, biogeochemistry, and advanced analytical chemistry, a biogeochemical hot spot was revealed in the mesotelm (30- to 50-cm depth) as a pronounced shift in microbial community composition in parallel with elevated peat decomposition. The relative abundance ofAcidobacteriaand theSyntrophobacteraceae, including known hydrocarbon-utilizing genera, was positively correlated with carbohydrate and organic acid content, showing a maximum in the mesotelm. The abundance ofArchaea(primarily crenarchaeal groups 1.1c and 1.3) increased with depth, reaching up to 60% of total small-subunit (SSU) rRNA gene sequences in the deep peat below the 75-cm depth. Stable isotope geochemistry and potential rates of methane production paralleled vertical changes in methanogen community composition to indicate a predominance of acetoclastic methanogenesis mediated by theMethanosarcinalesin the mesotelm, while hydrogen-utilizing methanogens predominated in the deeper catotelm. RNA-derived pyrosequence libraries corroborated DNA sequence data to indicate that the above-mentioned microbial groups are metabolically active in the mid-depth zone. Fungi showed a maximum in rRNA gene abundance above the 30-cm depth, which comprised only an average of 0.1% of total bacterial and archaeal rRNA gene abundance, indicating prokaryotic dominance. Ratios of C to P enzyme activities approached 0.5 at the acrotelm and catotelm, indicating phosphorus limitation. In contrast, P limitation pressure appeared to be relieved in the mesotelm, likely due to P solubilization by microbial production of organic acids and C-P lyases. Based on path analysis and the modeling of community spatial turnover, we hypothesize that P limitation outweighs N limitation at MEF, and microbial communities are structured by the dominant shrub,Chamaedaphne calyculata, which may act as a carbon source for major consumers in the peatland.

scholarly journals Heavy Metal Tolerance Genes Associated With Contaminated Sediments From an E-Waste Recycling River in Southern China

2021 ◽  
Vol 12 ◽  
Author(s):  
Shengqiao Long ◽  
Hui Tong ◽  
Xuxiang Zhang ◽  
Shuyu Jia ◽  
Manjia Chen ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Microbial Community ◽  
Microbial Communities ◽  
Southern China ◽  
Risk Index ◽  
River Sediments ◽  
Microbial Community Composition ◽  
Heavy Metal Tolerance ◽  
Waste Recycling ◽  
The Impact

Heavy metal pollution that results from electronic waste (e-waste) recycling activities has severe ecological environmental toxicity impacts on recycling areas. The distribution of heavy metals and the impact on the bacteria in these areas have received much attention. However, the diversity and composition of the microbial communities and the characteristics of heavy metal resistance genes (HMRGs) in the river sediments after long-term e-waste contamination still remain unclear. In this study, eight river sediment samples along a river in a recycling area were studied for the heavy metal concentration and the microbial community composition. The microbial community consisted of 13 phyla including Firmicutes (ranging from 10.45 to 36.63%), Proteobacteria (11.76 to 32.59%), Actinobacteria (14.81 to 27.45%), and unclassified bacteria. The abundance of Firmicutes increased along with the level of contaminants, while Actinobacteria decreased. A canonical correspondence analysis (CCA) showed that the concentration of mercury was significantly correlated with the microbial community and species distribution, which agreed with an analysis of the potential ecological risk index. Moreover, manually curated HMRGs were established, and the HMRG analysis results according to Illumina high-throughput sequencing showed that the abundance of HMRGs was positively related to the level of contamination, demonstrating a variety of resistance mechanisms to adapt, accommodate, and live under heavy metal-contaminated conditions. These findings increase the understanding of the changes in microbial communities in e-waste recycling areas and extend our knowledge of the HMRGs involved in the recovery of the ecological environment.

scholarly journals Microbial diversity in intensively farmed lake sediment contaminated by heavy metals and identification of microbial taxa bioindicators of environmental quality

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
María Custodio ◽  
Ciro Espinoza ◽  
Richard Peñaloza ◽  
Tessy Peralta-Ortiz ◽  
Héctor Sánchez-Suárez ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Microbial Diversity ◽  
Microbial Communities ◽  
Lake Sediments ◽  
Environmental Quality ◽  
Heavy Metal Contamination ◽  
Current Knowledge ◽  
Freshwater Ecosystems ◽  
Monitoring And Control

AbstractThe cumulative effects of anthropogenic stress on freshwater ecosystems are becoming increasingly evident and worrisome. In lake sediments contaminated by heavy metals, the composition and structure of microbial communities can change and affect nutrient transformation and biogeochemical cycling of sediments. In this study, bacterial and archaeal communities of lake sediments under fish pressure contaminated with heavy metals were investigated by the Illumina MiSeq platform. Despite the similar content of most of the heavy metals in the lagoon sediments, we found that their microbial communities were different in diversity and composition. This difference would be determined by the resilience or tolerance of the microbial communities to the heavy metal enrichment gradient. Thirty-two different phyla and 66 different microbial classes were identified in sediment from the three lagoons studied. The highest percentages of contribution in the differentiation of microbial communities were presented by the classes Alphaproteobacteria (19.08%), Cyanophyceae (14.96%), Betaproteobacteria (9.01%) y Actinobacteria (7.55%). The bacteria that predominated in sediments with high levels of Cd and As were Deltaproteobacteria, Actinobacteria, Coriobacteriia, Nitrososphaeria and Acidobacteria (Pomacocha), Alphaproteobacteria, Chitinophagia, Nitrospira and Clostridia (Tipicocha) and Betaproteobacteria (Tranca Grande). Finally, the results allow us to expand the current knowledge of microbial diversity in lake sediments contaminated with heavy metals and to identify bioindicators taxa of environmental quality that can be used in the monitoring and control of heavy metal contamination.

scholarly journals Ecological Effects of Heavy Metal Pollution on Soil Microbial Community Structure and Diversity on Both Sides of a River around a Mining Area

2020 ◽  
Vol 17 (16) ◽  
pp. 5680
Author(s):  
Xingqing Zhao ◽  
Jian Huang ◽  
Xuyan Zhu ◽  
Jinchun Chai ◽  
Xiaoli Ji
Keyword(s):  
Heavy Metal ◽  
Community Structure ◽  
Microbial Community ◽  
Environmental Factors ◽  
Microbial Communities ◽  
Microbial Community Structure ◽  
Metal Pollution ◽  
Heavy Metal Pollution ◽  
Mining Area ◽  
Soil Microbial

The objectives of this study were to understand the characteristics of heavy metal pollution caused by mining activities on the two sides of the Shun’an river and the response of soil microorganisms to the habitats by different contamination levels and vegetation. This paper selected soil samples from the banks of the Shun’an River near the Shizishan mining area, which is at the left of the river, in Tongling, Anhui Province, China. Using Illumina MiSeq 2500 technology, we analyzed the relationship between environmental factors and microbial communities. As the distance from the mining area increased, the heavy metal comprehensive pollution and potential risk value decreased. Additionally, the pollution severity and risk value of the left bank, where the mining area lies, were generally higher than those of the right bank. Because the symmetric sampling points on both banks of the river had similar planting types, their environmental factors and microbial community structure were similar and clustered. However, under different vegetation, the paddy soils tended to have a higher nutrient content and community richness and diversity than the vegetable fields or the abandoned land. It was found that soil microbial communities in this area were mostly affected by pH and Nemerow pollution index (PN). The pH significantly affected the abundance and structure of most microorganisms. In addition, Proteobacteria, Acidobacteria, and Bacteroidetes had significant tolerance to Zn, Pb, and Cd. By exploring the potential use of these tolerant microorganisms, we seek to provide strains and the theoretical basis for the bioremediation of areas contaminated by heavy metal.

scholarly journals Determining Rates of Change and Evaluating Group-Level Resiliency Differences in Hyporheic Microbial Communities in Response to Fluvial Heavy-Metal Deposition

2004 ◽  
Vol 70 (8) ◽  
pp. 4756-4765 ◽  
Author(s):  
Kevin P. Feris ◽  
Philip W. Ramsey ◽  
Matthias Rillig ◽  
Johnnie N. Moore ◽  
James E. Gannon ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Microbial Community ◽  
Microbial Communities ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Indigenous Populations ◽  
Curvilinear Relationship ◽  
Group Level ◽  
Rates Of Change

ABSTRACT Prior field studies by our group have demonstrated a relationship between fluvial deposition of heavy metals and hyporheic-zone microbial community structure. Here, we determined the rates of change in hyporheic microbial communities in response to heavy-metal contamination and assessed group-level differences in resiliency in response to heavy metals. A controlled laboratory study was performed using 20 flowthrough river mesocosms and a repeated-measurement factorial design. A single hyporheic microbial community was exposed to five different levels of an environmentally relevant metal treatment (0, 4, 8, 16, and 30% sterilized contaminated sediments). Community-level responses were monitored at 1, 2, 4, 8, and 12 weeks via denaturing gradient gel electrophoresis and quantitative PCR using group-specific primer sets for indigenous populations most closely related to the α-, β-, and γ-proteobacteria. There was a consistent, strong curvilinear relationship between community composition and heavy-metal contamination (R 2 = 0.83; P < 0.001), which was evident after only 7 days of metal exposure (i.e., short-term response). The abundance of each phylogenetic group was negatively affected by the heavy-metal treatments; however, each group recovered from the metal treatments to a different extent and at a unique rate during the course of the experiment. The structure of hyporheic microbial communities responded rapidly and at contamination levels an order of magnitude lower than those shown to elicit a response in aquatic macroinvertebrate assemblages. These studies indicate that hyporheic microbial communities are a sensitive and useful indicator of heavy-metal contamination in streams.

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