scholarly journals Genetic determinants of HSP70 gene expression following heat shock

2010 ◽  
Vol 19 (24) ◽  
pp. 4939-4947 ◽  
Author(s):  
Narelle Maugeri ◽  
Jayachandran Radhakrishnan ◽  
Julian C. Knight
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Hsp70 Gene ◽  
Genetic Determinants

Regulation of chemical stress-induced hsp70 gene expression in murine L929 cells

1994 ◽  
Vol 107 (8) ◽  
pp. 2209-2214 ◽  
Author(s):  
R.Y. Liu ◽  
P.M. Corry ◽  
Y.J. Lee
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Negative Regulator ◽  
Transcriptional Level ◽  
Regulation Mechanism ◽  
Chemical Stress ◽  
Hsp70 Gene ◽  
Heat Shock Element ◽  
L929 Cells ◽  
Chemical Treatments

We have investigated the regulation mechanism of chemical stress-induced hsp70 gene expression in murine L929 cells. Our data show that chemical treatments including sodium arsenite, cadmium chloride and sodium salicylate, induced significant synthesis of hsp70 and its mRNA. The induced hsp70 gene expression appears to be regulated at the transcriptional level. A factor (CHBF), which constitutively binds to the heat shock element (HSE) at 37 degrees C, functions like a negative regulator and the heat-induced heat shock factor (HSF) acts as an activator. The chemical treatments that induce significant hsp70 synthesis activate HSF binding to HSE but also dissociate the HSE-CHBF complex. Some chemical treatments, e.g. IPTG, which fail to activate hsp70 gene transcription, still activate HSF binding to HSE. However, in this case, the HSE-CHBF complex remained like that of untreated control cells.

Modulation of Heat-Shock Protein 70 (HSP70) Gene Expression by Sodium Butyrate in U-937 Promonocytic Cells: Relationships with Differentiation and Apoptosis

1997 ◽  
Vol 236 (1) ◽  
pp. 268-274 ◽  
Author(s):  
Laura Garcia-Bermejo ◽  
Nuria E. Vilaboa ◽  
Concepcion Perez ◽  
Alba Galan ◽  
Elena De Blas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Sodium Butyrate ◽  
Heat Shock Protein 70 ◽  
Hsp70 Gene ◽  
Promonocytic Cells

scholarly journals Mitigation of Heat Stress in Broiler Chickens with Heat Shock Protein 70 Gene Expression as its Indicator

2020 ◽  
Vol 30 (4) ◽  
pp. 177
Author(s):  
Cecep Hidayat ◽  
Komarudin . ◽  
E Wina
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Production Performance ◽  
Hsp70 Gene ◽  
Hsp 70 ◽  
Manganese Chromium

<p class="awabstrak2"><span lang="EN-US">Heat stress is an important issue in broiler chicken farms in tropical countries, such as Indonesia. Heat stress is very detrimental to broiler chickens because reducing production performance, health, and causing mortality. In the condition of heat stress, broilers synthesize Heat Shock Protein (HSP) quickly as the body's response to heat stress. HSP 70 is the most studied HSP group related to heat stress. The objective of this study was to review the nutritional approach that has been done to mitigate heat stress in broiler chickens with the HSP70 gene expression as its indicator. Based on some studies, nutritional approaches that can be taken are through the management of feed availability, supplementation of vitamin C, vitamin E, plant bioactives, amino acids (taurine and glutamine), probiotics, prebiotics, synbiotics, manan oligo saccharide (MOS) and minerals (selenium, zinc, manganese, chromium). By these approaches, HSP70 gene expression decreased indicating that the heat stress level of broiler chicken also reduced. It can be concluded that the nutritional approach can be used as a method for heat stress mitigation in broilers with the HSP70 gene expression indicator. </span></p>

scholarly journals Effect of betaine on HSP70 expression and cell survival during adaptation to osmotic stress

1993 ◽  
Vol 293 (2) ◽  
pp. 553-558 ◽  
Author(s):  
P G Petronini ◽  
E M De Angelis ◽  
A F Borghetti ◽  
K P Wheeler
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Osmotic Stress ◽  
Cell Survival ◽  
Osmotic Pressure ◽  
Colony Formation ◽  
Hsp70 Expression ◽  
Hsp70 Gene ◽  
3T3 Cells ◽  
Induced Expression

Induced expression of the HSP70 gene in 3T3 and SV-3T3 cells was monitored by measurements of the synthesis of HSP70 and of the cellular contents of both HSP70 and its mRNA. The presence of betaine (N-trimethylglycine) at concentrations of 2.5-25 mM decreased the induction of HSP70 gene expression caused by incubation of 3T3 and SV-3T3 cells in hypertonic (0.5 osM) medium. This effect was accompanied by an enhancement of SV-3T3 cell adaptation, assayed by colony formation, to the hyperosmotic conditions. In contrast, the presence of betaine did not affect HSP70 gene expression induced in these cells by heat shock. After 6 h incubation with 25 mM betaine under hypertonic (0.5 osM) conditions the intracellular concentration of betaine in SV-3T3 cells was about 195 mM, compared with about 70 mM under isotonic (0.3 osM) conditions. Hence, with this concentration of extracellular betaine, the marked increase in the accumulation of betaine within the cells presumably counteracts the imposed osmotic pressure and eliminates the signal that otherwise initiates increased expression of the HSP70 gene.

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