P–188 Ambient light intensity and wavelength in the IVF laboratory does not affect life birth rates

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Farrera. Ayestaran ◽  
V Montalvo ◽  
J Masso ◽  
A Garcia-Faura ◽  
B Marques ◽  
...  
Keyword(s):  
Light Intensity ◽  
Clinical Outcomes ◽  
Light Exposure ◽  
Time Lapse ◽  
Control Group ◽  
Ambient Light ◽  
Ambient Illumination ◽  
Birth Rates ◽  
Lighting Conditions ◽  
Ambient Lighting

Abstract Study question Do different wavelengths and intensities in ambient lighting affect clinical outcomes? Summary answer Variations on ambient lighting intensity and wavelength do not affect life birth rates. What is known already Light is one of the factors to consider when designing an IVF laboratory. Most IVF clinics work under reduced illumination, trying to mimic uterine conditions as much as possible. Nevertheless, it has been described that 95% of the light that affects an embryo comes from the microscope, not ambient lighting. It is well accepted that exposure to extreme lighting conditions affects embryos through photo-oxidation and the creation of reactive oxygen species. Still, there is no study that documents the effect of different wavelengths on human embryos. Study design, size, duration Prospective study performed between january 2019 and february 2020. Every 60 days we changed ambient illumination conditions using the LED lighting installed throughout the IVF laboratory. Six different groups were created: Cyan (470nm), Green (550nm), Yellow (600nm), Orange (625nm), intense white (WH), and low intensity white (WL) as control group. Participants/materials, setting, methods A total of 572 egg donation cicles with 355 fresh single embryo transfers were included in the study. In all cycles ICSI and Time-lapse culture was performed (Embryoscope, Vitrolife). PGT and testicular biopsy/aspiration treatments were excluded. Eggs and embryos were exposed to ambient illumination during pick-up, denudation, ICSI, and embryo transfer procedures. Main results and the role of chance Light exposure during embryo/gamete manipulation is inevitable. Hence, we analyzed parameters linked to the success of an IVF cycle to assess the effect of different lighting conditions concluding that neither light color nor intensity affect IVF success rates. No differences were found between groups regarding maternal age, age of the recipient, diagnostic, or number of eggs received (p > 0.05). Fertilization rates were similar between groups (C = 77.04%; G = 73.72%; Y = 75.64%; O = 78.1%; WL = 76.4%; WH = 75.2%; p = 0.216) as well as good quality blastocyst rates (C = 57.35%; G = 57.37%; Y = 62.30%; O = 59.75%; WL = 63.28%; WH = 60.55%; p = 0.234). Regarding clinical outcomes both implantation and miscarriage rates were found to be equal between groups (C = 61.67%; G = 52.89%; Y = 55.10%; O = 66.18%; WL = 66.00%; WH = 53.55%; p = 0.194, and C = 24.32%; G = 19.15%; Y = 11.11%; O = 24.44%; WL = 15.15%; WH = 8.11%; p = 0.301). The main outcome for this study was live birth rates and no differences were found (C = 51.85%; G = 50.00%; Y = 52.17%; O = 53.97%; WL = 57.14%; WH = 50.75%; p = 0.168). Limitations, reasons for caution We must take into account that embryos were cultured inside a time-lapse incubator, diminishing the effect of ambient light. Wider implications of the findings: This study demonstrates that, with advances in culture technology, neither light intensity nor light wavelength affecting gametes/embryos during manipulation influence clinical outcomes. Trial registration number Not applicable

A Practical Tool for Ambient Illumination Comparisons at Dusk/Dawn

2016 ◽  
Vol 60 (1) ◽  
pp. 470-474 ◽  
Author(s):  
Hilary Lam ◽  
Sayf Gani ◽  
Randy Mawson ◽  
Jason Young ◽  
Erin Potma
Keyword(s):  
Human Perception ◽  
Time Lapse ◽  
Time Of Day ◽  
Ambient Illumination ◽  
Adjustment Factor ◽  
Lighting Conditions ◽  
Accurate Knowledge ◽  
Ambient Lighting ◽  
Practical Tool ◽  
Sky Conditions

Nighttime visibility is an important consideration in collision reconstruction and personal injury investigation. Decreased contrast in low ambient lighting conditions can greatly affect human perception and response. Because ambient lighting levels change rapidly at dawn and dusk, forensic investigators must have an accurate knowledge of the time of day and the cloud conditions at the time of the incident before initiating a nighttime visibility assessment. Previously, human factors experts attempting re-enactments at dawn or dusk have had to wait for sky conditions that match those at the time of the incident, making the investigation of those cases extremely difficult, if not unfeasible. In this study, an ambient illumination equivalency tool has been developed based on a database of time-lapse light meter readings collected by the authors. This new tool can be used to facilitate nighttime visibility assessments on any day by providing a time adjustment factor to account for the changes in ambient illuminance due to differences in the cloud conditions between the day of the incident and the day of the re-enactment.

P–224 Multinucleated and non-multinucleated embryos in PGT-A cycles: Is there any difference?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M I Papadopoulou ◽  
A Papatheodorou ◽  
A Vorniotaki ◽  
N Christoforidis ◽  
A Chatziparasidou
Keyword(s):  
Clinical Outcome ◽  
Clinical Outcomes ◽  
Chromosomal Abnormality ◽  
Time Lapse ◽  
Control Group ◽  
Blastocyst Formation ◽  
Cleavage Stage ◽  
Female Age ◽  
And Control ◽  
Euploidy Rate

Abstract Study question Is multinucleation during cleavage stage correlated with the ploidy status of embryos and how does it affect the clinical outcome? Summary answer The presence of multinucleated embryos does not affect clinical outcome, although the risk of aneuploidy is higher in multinucleated embryos. What is known already Multinucleated blastomeres (ΜΝ) of cleavage stage embryos has been reported widely in scientific literature. Multinucleation has been associated with diminished embryo developmental competency and clinical outcomes such as lower implantation. Although this is an intriguing subject of research, it is not clear yet whether multinucleation is related to aneuploidy. Morphological irregularities such as multinucleation in blastomeres became a constant finding only after the perpetually evolving technology of time-lapse culture of embryos which in combination with PGT-A analysis, creates new research paths which aim to develop a new tool for selection or deselection of embryos for transfer. Study design, size, duration This retrospective study, included 97 PGT-A cycles, performed at Embryolab fertility clinic from May 2017 to December 2020, all cultured in time-lapse incubator (EmbryoScope). Two study groups were formed; the MN Group consisted of PGT-A cycles with at least one multinucleated embryo (n = 56) and the Control Group in which all PGT-A cycles had no multinucleated embryos (n = 38). Euploidy rate, type of chromosomal abnormality, cumulative pregnancy and live birth rates were compared between the two groups. Participants/materials, setting, methods Embryos were annotated for the existence of multinucleated blastomeres on Day 2 of their development. Biopsy was performed on Days 5/6 and embryos were genetically tested. One or two euploid embryos were transferred. Euploidy rate and clinical outcomes between the two groups were compared. Within the MN group, euploidy rate between multinucleated and non-multinucleated embryos was compared. For abnormal embryos, association of multinucleation with the type of abnormality was tested. SAS statistical analysis was performed. Main results and the role of chance Mean female age was 35.93 years in the MN group and 38.39 years in the control group. Blastocyst formation rate (expressed per fertilised oocytes) was similar between MN and Control group (74% vs 76%, p = 0.6303). In the MN group, 56 cases resulted in 44 embryo transfers while in the control group 38 cases resulted in 23 embryo transfers. Pregnancy rates (59.09% vs 65.21%, p = 0.6255) and clinical pregnancy rates (45.45% vs 39.13%, p = 0.4245) were not significantly different between MN and Control group. Initially, cumulative live birth rate was found to be significantly higher in the MN group compared to the Control group (62.96% vs 33.34%, p = 0.0417). However, when logistic and poisson regression was applied, it became obvious that this difference was not affected by multinucleation but from other factors such as female age. When comparing multinucleated and non-multinucleated embryos within the MN group, it was found that the mean number of euploid embryos was significantly higher in the non-multinucleated subgroup of embryos (p = 0.0021). No correlation was found between multinucleation and the type of chromosomal abnormality. Limitations, reasons for caution The sample size is the main limitation of the present study. More research with bigger sample size is needed in order to confirm the finding of the present study. Wider implications of the findings: The present study suggests that multinucleated blastomeres during embryo development is not an indication for diminished blastocyst formation and does not affect the clinical outcomes. However, within a sibling embryo population, non-multinucleated embryos tend to be euploid and this finding can be used to advance embryo selection efficiency. Trial registration number Not applicable

Range of entrainment of rat circadian rhythms to sinusoidal light-intensity cycles

2000 ◽  
Vol 278 (5) ◽  
pp. R1148-R1156 ◽  
Author(s):  
Setsuo Usui ◽  
Yasuro Takahashi ◽  
Terue Okazaki
Keyword(s):  
Circadian Rhythms ◽  
Light Intensity ◽  
Lower Limit ◽  
Phase Angle ◽  
Light Exposure ◽  
Sprague Dawley ◽  
Lighting Conditions ◽  
Behavioral Rhythm ◽  
Sprague Dawley Rats ◽  
Daily Cycles

The range of entrainment of the circadian behavioral rhythm was compared between two groups of Sprague-Dawley rats (each n = 10) exposed to daily cycles of rectangular light-dark alternation (LD) and sinusoidal fluctuations of light intensity (SINE), respectively. The maximum illuminance (20 lx), the minimum illuminance (0.01 lx), and the total amount of light exposure per cycle were the same under the two lighting conditions. The periods (Ts) of both lighting cycles were lengthened stepwise from 24 through 25, 26, 26.5, 27, 27.5, and 28 h to 28.5 h in experiment 1 and were shortened stepwise from 24 through 23.5, 23, and 22.5 h to 22 h in experiment 2. Each T cycle lasted for 30 cycles. In experiment 1, 60% of rats under the LD condition entrained up to T = 28.5 h, whereas 50% of rats under the SINE condition entrained up to T = 28.5 h. In experiment 2, no animal under the LD condition entrained to T < 23.5 h, whereas 40% of rats under the SINE condition entrained down to T = 23 h and 20% of rats remained to entrain down to T = 22 h cycles. The phase angle of entrainment was systematically changed, depending on T under both conditions. These results suggest that the lower limit of entrainment is expanded under the SINE condition compared with the LD condition.

scholarly journals Night-time Lookout Duty: The Role of Ambient Light Levels and Dark Adaptation

2012 ◽  
Vol 65 (4) ◽  
pp. 589-602 ◽  
Author(s):  
Tony Wynn ◽  
Peter A. Howarth ◽  
Bert R. Kunze
Keyword(s):  
Dark Adaptation ◽  
Probability Of Detection ◽  
Working Environment ◽  
Ambient Light ◽  
Night Time ◽  
Lighting Conditions ◽  
Light Levels ◽  
Ambient Lighting ◽  
Small Craft

The aim of this research was to clarify and quantify the demands of the working environment and watch-keeping regime for large commercial ships in relation to dark adaptation. The night lookout task requires the identification of the relatively bright navigational lights of other ships against the dark background of the sky and sea. The probability of detection is determined by the ambient lighting conditions on the bridge and the dark adapted state of vision. Light levels were such that threshold sensitivity (after 15 minutes) was reduced by around 2 log units in comparison to complete darkness. This has implications for the effective range of navigational lights at sea as defined in regulations. The intensity and position of navigation lights on larger vessels is such that the sensitivity of the eye under typical bridge conditions is likely to be sufficient for their visibility to be acceptable. This may not be the case for less well lit small craft.

scholarly journals Effect of TiO2 Nanoparticles on the Yield and Photophysiological Responses of Cherry Tomatoes during the Rainy Season

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 563
Author(s):  
Hyo Gil Choi
Keyword(s):  
Light Intensity ◽  
Tio2 Nanoparticles ◽  
Rainy Season ◽  
Co2 Fixation ◽  
Foliar Spray ◽  
Control Group ◽  
Ambient Light ◽  
Cherry Tomato ◽  
Ojip Transient ◽  
Nanoparticle Suspension

The rainy season occurs mainly from June to July in Korea, and this season causes insufficient ambient light intensity for the growth of cherry tomato in a greenhouse. Titanium dioxide (TiO2), as a photocatalyst, is known to affect photosynthesis in plants. This study was carried out to investigate the influence of TiO2 foliar spray application on the yield and photophysiological responses of cherry tomato under low ambient light intensity during the rainy season in a greenhouse. Cherry tomato plants were treated with 100 mg·L−1 TiO2 (T1) or 200 mg·L−1 TiO2 (T2) nanoparticle suspension on 26 June. The control group was not treated with TiO2. In the O–J phase of the OJIP transient under a cloudy day (2 July), the slope in the control and T1 groups rose more sharply than that in the T2 group. Conversely, on a clear day (10 July), the J–I phase of the T2 group sharply increased compared to that of the control and T1 groups. On a cloudy day with low ambient light intensity, the rate of electron transport flux from QA to QB per photosystem II reaction center (ET0/RC) and carbon dioxide (CO2) fixation of TiO2-treated plants were increased compared to those of the control. However, on a clear day of high light intensity, the ET0/RC and CO2 fixation of the T2 group were lower than those of the control and Tl groups. The yield of fruit was increased in the T1 group over that in other treatments. TiO2 treatment reduced the size of the fruit and delayed the ripening time, but greatly increased the fruit hardness. These results suggest that setting the concentration and supply amount of TiO2 nanoparticles suitable for various environmental conditions should be prioritized in order to improve the effect of TiO2 nanoparticles in tomato cultivation.

Convolutional neural network-based ambient light-independent panel digit surveillance technique for infusion pumps

2021 ◽  
pp. 095441192199609
Author(s):  
Young Jun Hwang ◽  
Gun Ho Kim ◽  
Eui Suk Sung ◽  
Kyoung Won Nam
Keyword(s):  
Neural Network ◽  
Convolutional Neural Network ◽  
Continuous Monitoring ◽  
Ambient Light ◽  
Infusion Pumps ◽  
Lighting Conditions ◽  
Ambient Lighting ◽  
Independent Panel ◽  
Monitoring Test

For effective patient therapy and improved patient safety, it is critical to administer medication accurately in accordance with doctor’s prescription. However, accidents owing to the erroneous programing of infusion pumps caused by users have been consistently reported in several documents. In this study, the authors propose a novel surveillance technique for infusion pumps to continuously monitor the variations in panel digits using a convolutional neural network model, and evaluate the performance of the implemented technique. During the experimental evaluation, 1st-step ROIs and 2nd-step ROIs were successfully extracted from the frame images regardless of the ambient lighting conditions. The final accuracies of the implemented CNN model are 99.9% for both the training (172,800 images) and validation (1080 images) dataset while the final losses for the training and validation datasets are 0.48 and 0.45 after 13th epoch, respectively. In the 24-h continuous monitoring test, the accuracy of the model for volume recognition considering all the 1440 measurements (960 for day-lighting and 480 for night-lighting) is 95.5%, whereas in day-lighting and night-lighting modes the accuracies of the model are 98.2% and 90.0%, respectively. Based on these experimental results, the proposed surveillance technique incorporating infusion pumps is expected to improve the safety of patients who need long-term treatments via infusion pumps, reducing the burden on the nurses and hospitals.

Measurement of the Opacity of Smoke and Suspensions under Ambient Lighting Conditions

1977 ◽  
Vol 10 (1) ◽  
pp. 2-3 ◽  
Author(s):  
A. R. Churchley
Keyword(s):  
Solid State ◽  
Light Emitting Diode ◽  
Reference Beam ◽  
Ambient Light ◽  
Entry And Exit ◽  
Optical Cell ◽  
Light Emitting ◽  
Lighting Conditions ◽  
Ambient Lighting ◽  
Scattering Systems

An entirely solid state photoelectric meter for the measurement of optical density is described. No light sealing arrangements are required, greatly simplifying the cleaning of the optical cell and facilitating the entry and exit of material when measurements are taken on flowing systems. Ambient light reaching the detector does not interfere with the measurements since the reference beam is coded. This beam is generated by a light emitting diode, avoiding many of the problems of conventional sources. The system has a wide application and its robustness should assure it a position in the field as well as the laboratory. Any change in the intensity of the reference beam can be measured, so the properties of transmissive, reflective or scattering systems may be measured.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

EXPRESS: Altering the rehabilitation environment to improve stroke survivor activity (AREISSA): a Phase II trial.

2021 ◽  
pp. 174749302110069
Author(s):  
Heidi Janssen ◽  
Louise Ada ◽  
Sandy Middleton ◽  
Michael Pollack ◽  
Michael Nilsson ◽  
...  
Keyword(s):  
Adverse Events ◽  
Clinical Outcomes ◽  
Environmental Enrichment ◽  
Brain Plasticity ◽  
Social Activity ◽  
Stroke Survivor ◽  
Group Differences ◽  
Control Group ◽  
Serious Adverse Events ◽  
Clinical Trial Registration

Background: Environmental enrichment involves organisation of the environment and provision of equipment to facilitate engagement in physical, cognitive and social activity. In animals with stroke, it promotes brain plasticity and recovery. Aims: To assess the feasibility and safety of a patient-driven model of environmental enrichment incorporating access to communal and individual environmental enrichment. Methods: A non-randomised cluster trial with blinded measurement involving people with stroke (n=193) in 4 rehabilitation units was carried out. Feasibility was operationalised as activity 10 days after admission to rehabilitation and availability of environmental enrichment. Safety was measured as falls and serious adverse events. Benefit was measured as clinical outcomes at 3 months, by an assessor blinded to group. Results: The experimental group (n=91) spent 7% (95% CI -14 to 0) less time inactive, 9% (95% CI 0 to 19) more time physically, and 6% (95% CI 2 to 10) more time socially active than the control group (n=102). Communal environmental enrichment was available 100% of the time, but individual environmental enrichment was rarely within reach (24%) or sight (39%). There were no between-group differences in serious adverse events or falls at discharge or 3 months nor in clinical outcomes at 3 months. Conclusions: This patient-driven model of environmental enrichment was feasible and safe. However, the very modest increase in activity by people with stroke, and the lack of benefit in clinical outcomes 3 months after stroke do not provide justification for an efficacy trial. Clinical Trial Registration: ANZCTR 12613000796785 Words: 245

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