Protein Analysis of Feeds, Using a Block Digestor

1975 ◽  
Vol 58 (1) ◽  
pp. 143-145 ◽  
Author(s):  
Larry G Hambleton ◽  
Rodney J Noel
Keyword(s):  
Cottonseed Meal ◽  
Soybean Meal ◽  
Blood Meal ◽  
Significant Difference ◽  
Kjeldahl Method ◽  
Student’S T ◽  
Feed Samples ◽  
Good Agreement ◽  
Student’S T Test

Abstract A method for the determination of proteins in feeds, utilizing the Technicon® BD-20 block digestor, has been developed. Twenty feed samples are weighed directly into the 250 ml tubes and digested for 35 min after clearing. The temperature of the solution during the digestion and the uniformity of temperature between various holes in the block were determined. The effect of air currents over the tubes was also studied. Six replicate determinations on cottonseed meal, blood meal, soybean meal, tryptophan, and 2 feed samples are reported and compared to results on the same sample analyzed by the official final action Kjeldahl method; good agreement was shown between the methods. The accuracy of the block digestor was confirmed by analyzing 40 routine feed samples by both the block digestor and Kjeldahl methods; results did not show a significant difference when compared by Student's t-test at the 5% level.

Comparison of Automated Method and Improved AOAC Kjeldahl Method for Determination of Protein in Meat and Meat Products

1981 ◽  
Vol 64 (1) ◽  
pp. 29-31 ◽  
Author(s):  
Debra L McGill
Keyword(s):  
Paired Comparison ◽  
Meat Products ◽  
Protein Determination ◽  
Automated Method ◽  
Significant Difference ◽  
Kjeldahl Method ◽  
Student’S T ◽  
Meat Samples ◽  
Student’S T Test

Abstract The Kjel-Foss automated method for protein determination in meat and meat products was compared with the improved AOAC Kjeldahl method. Meat samples were separated into 3 categories based on fat content and analyzed in duplicate by both methods. No significant difference was found in a paired comparison of the 2 methods in each of the 3 meat categories, using Student’s t-test at the 99% confidence level. A number of additional meat samples analyzed 6-9 times by the automated method showed an overall average range of 0.55% protein and average standard deviation of 0.20. The Kjel-Foss automated method was applicable for total protein determination in a wide variety of meat and meat products.

scholarly journals SEQUENTIAL MULTI-ELEMENT DETERMINATION OF TRACE-METALS IN BIODIESEL SAMPLES USING HR-CS FAAS

Química Nova ◽  
2020 ◽  
Author(s):  
Alexandre Jesus ◽  
Ariane Zmozinskia ◽  
Diane Laroque ◽  
Márcia Silva
Keyword(s):  
Absorption Spectrometry ◽  
Element Determination ◽  
Continuum Source ◽  
Flame Atomic Absorption ◽  
Student’S T ◽  
Different Sources ◽  
Certified Values ◽  
Good Agreement ◽  
Student’S T Test

In this work the feasibility of sequential multi-element determination of Zn, Fe, Mg, Cu, Na, K, Cr, Al and Ca in biodiesel samples by high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS) was investigated. Biodiesel samples obtained from different sources and different chemical processes were analysed. The samples were diluted with n-propanol and water (1.4 mol L-1 HNO3) to form a microemulsion (ME) before its introduction in the HR-CS FAAS using a flow injection mode. The sample B-01 (obtained from soybean oil) was used for optimisation of the instrumental parameters. The accuracy of the proposed method was checked by analysis of certified reference material (CRM) Conostan BDM2A (for K and Na) and BDM2B (for Ca and Mg) and by comparison with acid digestion and recovery tests (for Zn, Fe, Cu, Cr and Al). The obtained results for CRM analysis showed good agreement with certified values within 95% of confidence (Student’s t-test). Recovery tests values ranged from 87-114%. The method developed for the analysis of biodiesel samples is accurate, simple, fast, and suitable for routine application.

CuSO4-TiO2 as Kjeldahl Digestion Catalyst in Manual Determination of Crude Protein in Animal Feeds

1986 ◽  
Vol 69 (4) ◽  
pp. 664-666
Author(s):  
Peter F Kane
Keyword(s):  
Standard Deviation ◽  
Crude Protein ◽  
T Test ◽  
Environmental Concerns ◽  
Animal Feeds ◽  
Significant Difference ◽  
Kjeldahl Digestion ◽  
Student’S T ◽  
Student’S T Test

Abstract The official AOAC manual Kjeldahl methods for determining crude protein in animal feeds have several disadvantages. For the HgO catalyst method, there are environmental concerns and a lengthy digestion. For the CuS04 catalyst method, the digestion period is shorter, but still 90 min. A different catalyst combination, CuS04-Ti02, makes 40 min digestion feasible. Comparison of these catalysts on a group of representative feeds resulted in a mean difference, Cu-Ti minus HgO, of 0.034% protein. Standard deviation of the differences was 0.36. A Student’s t-test showed no significant difference. The method will be collaboratively studied.

scholarly journals Development of an Ultraviolet Spectrophotometric Method for the Determination of Ceftiofur Sodium Powder

2009 ◽  
Vol 92 (6) ◽  
pp. 1673-1680 ◽  
Author(s):  
marinês J E Souza ◽  
Natália Canedo ◽  
Paulo S Souza Filho ◽  
Ana M Bergold
Keyword(s):  
Spectrophotometric Method ◽  
Spectrophotometric Analysis ◽  
Uv Spectrophotometry ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Student’S T ◽  
Ceftiofur Sodium ◽  
Interday Precision ◽  
Student’S T Test

Abstract A UV spectrophotometric method was developed for determination of ceftiofur sodium in the drug substance and sterile powder for injection. The method validation, which yielded good results, included evaluation of the range, linearity, intraand interday precision, accuracy, recovery, specificity, robustness, LOQ, and LOD. The UV spectrophotometric determinations were performed at 292 nm. Good linearity was obtained between 2.5 and 20.0 g/mL. A prospective validation showed that the method is linear (r = 0.9999) and precise, with RSD values of 0.3 for product A and 0.4 for product B. The intraand interday precision values were <2 for all samples analyzed. Comparison of UV spectrophotometry and LC by analysis of variance and Student's t-test showed no significant difference between methodologies. Moreover, the accuracy and precision obtained with the UV method correlated well with the values obtained with the LC method, and this correlation suggests that UV spectrophotometric analysis can be an inexpensive, reliable, and less time-consuming alternative to chromatographic analysis. The results demonstrated the validity of the proposed method as a simple and useful alternative for the determination of ceftiofur in routine QC analyses.

scholarly journals GREEN AND SIMPLE UV-VISIBLE DIFFUSE REFLECTANCE AND TRANSMITTANCE METHODS FOR THE DETERMINATION OF NITROFURANTOIN IN PHARMACEUTICAL PREPARATIONS

2017 ◽  
Vol 36 (4) ◽  
pp. 62 ◽  
Author(s):  
M. Tubino ◽  
L. F. Bianchessi ◽  
M. Palumbo ◽  
M. M.D.C. Vila
Keyword(s):  
Diffuse Reflectance ◽  
Pharmaceutical Preparations ◽  
The Other ◽  
Statistical Comparison ◽  
Ultraviolet Range ◽  
Uv Visible ◽  
Student’S T ◽  
Good Agreement ◽  
Student’S T Test

Two quantitative analytical methods for the determination of nitrofurantoin in pharmaceutical preparations in the visible-ultraviolet range of the spectrum are reported. One of them uses the diffuse reflectance technique and the other transmittance measurements in aqueous solution. Both procedures are very simple to be performed and also present green characteristics as only polyethylene glycol and water are used as solvents. Commercial pharmaceutical preparations of the drug were analyzed using the proposed methods. The results were compared with those obtained when the HPLC procedure recommended by the USP was applied. Statistical comparison using the paired Student’s t test and the Snedecor’s F test showed good agreement between all the methods.

Comparison of Rapid High Pressure Liquid Chromatographic and CB Methods for Determination of Aflatoxins in Corn and Peanuts

1982 ◽  
Vol 65 (2) ◽  
pp. 206-209
Author(s):  
Jonathan W Devries ◽  
Henry L Chang
Keyword(s):  
High Pressure ◽  
Rapid Determination ◽  
Peanut Butter ◽  
Peanut Meal ◽  
High Pressure Liquid Chromatographic ◽  
Significant Difference ◽  
Student’S T ◽  
Liquid Chromatographic ◽  
Student’S T Test

Abstract A method is described for the rapid determination of aflatoxins in corn and peanut samples by high pressure liquid chromatography. The method was compared with the current CB method (AOAC 26.026). For 7 samples of corn and 14 samples of peanut meal and peanut butter, the correlation between methods is 0.991, and no significant difference exists between methods, using the Student's t-test at 15.7% a-risk.

scholarly journals Determination of Salicylate in Blood Serum by Flow Injection with Immobilized Salicylate Hydroxylase

2001 ◽  
Vol 84 (5) ◽  
pp. 1363-1370 ◽  
Author(s):  
Marta M D C Vila ◽  
Matthieu Tubino ◽  
Graciliano de Oliveira Neto
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Enzymatic System ◽  
Serum Samples ◽  
Colored Product ◽  
The Mean ◽  
Student’S T ◽  
Good Agreement ◽  
Student’S T Test

Abstract A flow injection (FI) enzymatic system, based on the use of immobilized salicylate hydroxylase in glass beads, was developed for the determination of salicylate. Salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH) are used to convert salicylate to catechol. The reaction of catechol with 4-aminophenol at high pH yields a colored product which is detected spectrophotometrically at 565 nm. Ten samples of human serum containing from 5.0 × 10−4 to 5.0 × 10−3 mol/L added salicylate were analyzed and the recovery was determined. Eight additional serum samples containing salicylate were analyzed by the Trinder test and the proposed method. The results obtained with the 2 methods showed good agreement by the statistical Student's t-test. The relative precision of the method is about 3.4% (RSD of the mean recovery). Considering the lowest concentration analyzed, the quantitative limit of detection is about 0.2 × 10−5 mol/L (3 × SD). The volume of the sample used was 150 μL. The proposed method was also used to analyze medicines containing acetylsalicylic acid. The results were statistically compared with those obtained through the U.S. Pharmacopoeia procedure and showed excellent agreement.

scholarly journals ASSOCIATION BETWEEN SERUM URIC ACID AND METABOLIC SYNDROME

2018 ◽  
Vol 11 (10) ◽  
pp. 400 ◽  
Author(s):  
Thaslima Nandhini Js ◽  
Savitha Basker G ◽  
Vishnupriya V
Keyword(s):  
Metabolic Syndrome ◽  
Uric Acid ◽  
Venous Blood ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Serum Triglycerides ◽  
Significant Difference ◽  
Disease Condition ◽  
Student’S T ◽  
Student’S T Test

Objective: Metabolic syndrome is a cluster of disease condition characterized by truncal obesity, hypertriglyceridemia, elevated blood pressure, and insulin resistance. An excessive circulating uric acid (UA) level even within normal range is always comorbid with metabolic syndrome and its components. The aim of the current study was to investigate the association between metabolic syndrome and serum UA level.Methods: A total of 60 subjects were divided into two groups of healthy (30 individuals) and metabolic syndrome patients (30 individuals) from dental outpatient department of Saveetha Dental College and Hospitals. 5 ml of fasting venous blood was collected in the plain collection tubes and centrifuged, and then serum was separated. Then, the serum was used to analyze the fasting blood glucose, serum triglycerides (TGLs), and serum UA by GOD-POD, enzymatic colorimetric, and uricase method, respectively. A statistical analysis was performed using Student’s t-test. p<0.05 was considered to be statistically significant.Result: Mean body mass index (BMI), fasting blood sugar (FBS), TGL, and UA level of control group were 23.36±1.81, 84.45±13.1, 110.9±22.6, and 3.48±1.21 respectively. Mean BMI, FBS, TGL, and UA level of study group were 35.24±3.04, 122.85±23.3, 212.1±39.6 and 9.08±2.63 respectively. There is a significant difference between these two groups with p<0.0001.Conclusion: This study showed that those individuals with metabolic syndrome have higher UA level that indicates hyperuricemia which is a significant predictor of metabolic syndrome.

scholarly journals Effect of the ketoprofen on the preemptive analgesia in female cats undergoing to ovariohysterectomy

Revista CERES ◽  
2017 ◽  
Vol 64 (1) ◽  
pp. 18-24
Author(s):  
Dayvid Vianêis Farias de Lucena ◽  
Fernanda Vieira Henrique ◽  
Amara Gyane Alves de Lima ◽  
Almir Pereira de Souza ◽  
Pedro Isidro da Nóbrega Neto
Keyword(s):  
Statistical Analysis ◽  
Preemptive Analgesia ◽  
Pain Scores ◽  
Significant Difference ◽  
Reduction Of Pain ◽  
Baseline Values ◽  
Student’S T ◽  
Level Of Significance ◽  
Postoperative Administration ◽  
Student’S T Test

ABSTRACT The objective of this study was to investigate the effect of the ketoprofen on the preemptive analgesia in female cats submitted to ovariohysterectomy. Sixteen healthy cats were distributed into two groups, with eight animals each, by means of a draw, in a preemptive group (PREG), in which the animal received ketoprofen (1 mg/kg) subcutaneously two hours before the surgery; and postsurgery group (POSG), in which ketoprofen (1 mg/kg) was administered subcutaneously immediately after surgery. In both groups, ketoprofen was given 24, 48 and 72 hours after the first adminstration. Heart and respiratory frequencies and glycemia were measured in all animals during the days first ten postsurgery. Analgesia was measured by assigning scores at zero, two, four, six, 12, 24, 48, 72, 96, 120, 144, 168, 192, 216 and 240 hours after the surgical procedure. Statistical analysis was performed using two-way analysis of variance followed by Tukey's test to compare the moments and Student's t-test for comparison between groups. Pain scores were evaluated using test of U-Mann-Withney or Kruskall-Wallis, all at the 5% level of significance. Pain scores were significantly lower in PREG in M0, M6, M72, M96 and M120. No significant difference was found in the levels of glycemia in comparison to baseline values. Ketoprofen promotes postsurgery analgesia in female cats submitted to ovariohysterectomy and preemptive and postsurgery administration provides an earlier reduction of pain scores when compared to postoperative administration, only.

scholarly journals The nasal index in brazilian human skeletons

2018 ◽  
Vol 17 ◽  
pp. e18337 ◽  
Author(s):  
Larissa Chaves Cardoso Fernandes ◽  
Marcus Vitor Diniz de Carvalho ◽  
Eduardo Daruge Júnior ◽  
Luiz Francesquini Júnior ◽  
Patrícia Moreira Rabello ◽  
...  
Keyword(s):  
Mixed Race ◽  
Cross Sectional Study ◽  
Brazilian Population ◽  
Cross Sectional ◽  
Significance Level ◽  
Human Skulls ◽  
Student’S T ◽  
Digital Caliper ◽  
Student’S T Test

Aim: To investigate the percentage of correctness (PC) of the Nasal Index (NI) in human skeletons for determination of sex, ancestry and estimation of age in the Brazilian population. Methods: This was a cross-sectional study of 173 human skulls belonging to the Piracicaba Dental School. 93 skeletons were males and 80 females; 34 were aged up to 39 years, 56 between 40 and 59 years, and 83 60 years or older; 96 were from white individuals, 49 were from mixed-race and 28 from black individuals. High-precision digital caliper was used to measure nasal height (NH=ANS-nasion) and the maximum nasal width (NW) values, which were applied into the formula NI=NW/NHx100. The data were submitted to discriminant analysis and Student’s t test with equal variances, Mann-Whitney, F (ANOVA), Tukey and Kruskal Wallis, 5% significance level. Results: Dominant nasal type in the Brazilian population was the mesorine. Males showed nasal height and width values greater than those of females, with statistically significant differences in all measurements (p≤0.021) and PC of 76.6%. Similar nasal measurements were found regardless of age (p>0.05), with PC of 41.7%. Ancestral analysis revealed that black individuals have greater nasal width (26.35) and nasal index (53.67) than white ones (24.60 and 49.25), while mixed-race individuals showed intermediate values (25.36 and 52.13) (p<0.05). Nevertheless, these measurements presented an estimated PC of 54.3%. Conclusion: The Nasal Index can be better used for sex determination than for estimation of age and ancestry in the Brazilian population, as the latter showed intermediate and low percentage of correctness, respectively.

Sign in / Sign up

Export Citation Format

Share Document