scholarly journals RapidChek SELECTTMSalmonella Enteritidis Test System for the Detection of Salmonella Enteritidis in Poultry House Drag Swabs, Shell Egg Pools, and Chicken Carcass Rinsates

2011 ◽  
Vol 94 (4) ◽  
pp. 1138-1153 ◽  
Author(s):  
Mark T Muldoon ◽  
Verapaz Gonzalez ◽  
Meredith I Sutzko ◽  
Ann-Christine Olsson Allen ◽  
Samantha Creamer ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Reference Method ◽  
Test Strip ◽  
Test System ◽  
Test Method ◽  
Poultry House ◽  
Chicken Carcass ◽  
Two Samples ◽  
Secondary Enrichment ◽  
Inspection Service

Abstract The RapidChek SELECTTMSalmonella Enteritidis Test System was validated for the detection of Salmonella Enteritidis (SE) in poultry house drag swabs, shell egg pools, and chicken carcass rinsates. The method utilizes RapidChek SELECTTMSalmonella (AOAC PTM License No. 080601) proprietary primary and secondary enrichment media. Following enrichment, an immunochromatographic test strip is inserted into the tube containing the secondary enrichment broth, developed for 10 min, and interpreted. Salmonella Enteritidis-inoculated samples (1–5 CFU SE/analytical unit) were tested by the test method as well as the appropriate cultural reference method U.S. Food and Drug Administration-Bacteriological Analytical Manual (drag swabs and egg pools) or U.S. Department of Agriculture-Food Safety and Inspection Service (chicken carcass rinsates). A total of 80 samples were tested by both methods in the study. Fifty-two samples were positive by the RapidChek SELECT Salmonella Enteritidis method and 38 were found positive by the respective reference method. The sensitivity of the method was 100% and the specificity was 100%. The accuracy of the test method was 137%, indicating that the method was more sensitive than the reference method. The RapidChek SELECT Salmonella Enteritidis method was tested with 82 Salmonella Group D1 strains including 63 Salmonella Enteritidis strains as well as 32 non-Salmonella Group D1 strains representing 10 bacteria genera. The test method detected all 82 Group D1 strains (100% sensitivity). None of the non-Salmonella Group D1 or other genera of bacteria were detected, indicating a specificity of 100%. The method was shown to be highly robust and stable under control and accelerated stability conditions.

SDIX RapidChek™ Listeria F.A.S.T.™ Environmental Test System for the Detection of Listeria species on Environmental Surfaces

2012 ◽  
Vol 95 (3) ◽  
pp. 850-859 ◽  
Author(s):  
Mark T Muldoon ◽  
Anne-Christine Olsson Allen ◽  
Vera Gonzales ◽  
Meredith Sutzko ◽  
Klaus Lindpaintner
Keyword(s):  
Reference Method ◽  
Storage Conditions ◽  
Test System ◽  
Test Method ◽  
Broth Culture ◽  
Bacterial Strains ◽  
Chi Square ◽  
Listeria Species ◽  
Environmental Surfaces ◽  
Inspection Service

Abstract The SDIX RapidChekTMListeria F.A.S.T. test system was validated against the U. S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) cultural reference method for the detection of Listeria species on stainless steel, plastic, rubber, and painted concrete. The SDIX method uses a proprietary RapidChek Listeria enrichment media for a one-step, 24–40 h enrichment at 30°C, and detects Listeria on an immunochromatographic lateral flow device in 10 min. Different Listeria species were used to spike each of the environmental surfaces. Environmental surfaces were spiked at levels ranging from 50 to 400 CFU/surface (1 in.2 swabs for painted concrete, 4 in.2 for sponge). A total of 120 spiked samples were tested by the SDIX method at 24 and 40 h and the cultural reference method. Total confirmed positives were 49, 54, and 48 for the SDIX 24 h method, the SDIX 40 h method, and the USDA-FSIS cultural reference method, respectively. Nonspiked samples from all environmental surfaces were reported as negative for Listeria spp. by all methods. The overall Chi square was 0.017 (P = 0.104) and 0.611 (P = 0.566) after a 24 and 40 h enrichment, respectively, indicating that the test method was equivalent in performance to the reference method at both enrichment times. The SDIX method was evaluated for the detection of 50 Listeria and 35 non-Listeria bacterial strains. All 50 Listeria strains were detected by the method (100% sensitivity). Five out of 35 non-Listeria species gave light test signals when grown in nonselective broth culture and tested undiluted. However, when grown in the RapidChek Listeria F.A.S.T. proprietary media, only one bacterial strain (Staphylococcus aureus) was detected, giving a very low test signal (97% specificity). The method was shown to be robust toward several alterations in testing and storage conditions.

Validation of the Reveal® 2.0 Group D1 Salmonella Test for Detection of Salmonella Enteritidis in Raw Shell Eggs and Poultry-Associated Matrixes

2016 ◽  
Vol 99 (4) ◽  
pp. 1017-1024 ◽  
Author(s):  
Mark Mozola ◽  
Preetha Biswas ◽  
Ryan Viator ◽  
Emily Feldpausch ◽  
Debra Foti ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Probability Of Detection ◽  
Poultry Feed ◽  
Operating Parameters ◽  
Department Of Agriculture ◽  
Shell Eggs ◽  
Chicken Carcass ◽  
Inspection Service ◽  
The U.S ◽  
Culture Procedure

Abstract A study was conducted to assess the performance of the Reveal® 2.0 Group D1 Salmonella lateral flow immunoassay for use in detection of Salmonella Enteritidis (SE) in raw shell eggs and poultry-associated matrixes, including chicken carcass rinse and poultry feed. In inclusivity testing, the Reveal 2.0 test detected all 37 strains of SE tested. The test also detected all but one of 18 non-Enteritidis somatic group D1 Salmonella serovars examined. In exclusivity testing, none of 42 strains tested was detected. The exclusivity panel included Salmonella strains of somatic groups other than D1, as well as strains of other genera of Gram-negative bacteria. In matrix testing, performance of the Reveal 2.0 test was compared to that of the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook reference culture procedure for chicken carcass rinse and to that of the U.S. Food and Drug Administration Bacteriological Analytical Manual for raw shell eggs and poultry feed. For all matrixes evaluated, there were no significant differences in the ability to detect SE when comparing the Reveal 2.0 method and the appropriate reference culture procedure as determined by probability of detection statistical analysis. The ability of the Reveal 2.0 test to withstand modest perturbations to normal operating parameters was examined in robustness experiments. Results showed that the test can withstand deviations in up to three operating parameters simultaneously without significantly affecting performance. Real-time stability testing of multiple lots of Reveal 2.0 devices established the shelf life of the test device at 16 months postmanufacture.

Validation of the ANSR® for Campylobacter Method for Detection of Thermophilic Campylobacter spp. in Chicken Carcass Rinse and Turkey Sponge Samples

2016 ◽  
Vol 99 (6) ◽  
pp. 1555-1564 ◽  
Author(s):  
Ryan Viator ◽  
Susan Alles ◽  
Quynh-Nhi Le ◽  
Edan Hosking ◽  
Preetha Biswas ◽  
...  
Keyword(s):  
Target Genes ◽  
Reference Method ◽  
Common Species ◽  
Single Step ◽  
Fluorescence Detector ◽  
Method Performance ◽  
Chicken Carcass ◽  
Inspection Service ◽  
Nicking Enzyme ◽  
Performance Validation

Abstract A performance validation of the ANSR® for Campylobacter method was conducted in selected matrixes. This assay used selective nicking enzyme amplification technology to amplify target genes. Samples were enriched for 20 to 24 h and then lysed. The assay was completed within 50 min using real-time detection in a combination incubator/fluorescence detector and software. When 50 distinct strains of Campylobacter jejuni, C. lari, or C. coli were tested for inclusivity, all 50 strains produced positive results. In exclusivity testing, 31 strains of related organisms, including seven nontarget Campylobacter strains and other common species, were evaluated. All 31 species generated negative ANSR assay results, including the nontarget Campylobacter strains. The ANSR for Campylobacter method was compared to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook reference method using naturally contaminated chicken carcass rinse or turkey carcass sponge samples. ANSR method performance was not statistically different from the reference method using two different enrichment options. Equivalent results were observed at both time points (20 and 24 h) and in both atmospheres (microaerobic and aerobic) to reference methods. Method performance with chicken carcass rinse was confirmed in an independent laboratory study. Additionally, in robustness testing, small, deliberate changes to the assay parameters minimally affected ANSR method performance. Finally, accelerated stability results from three independently manufactured lots supported a shelf life of 6 months when stored at 4°C. The ANSR assay offered greater efficiency and flexibility when compared to the reference method with a 20–24 h single-step enrichment in a microaerobic or an aerobic atmosphere.

Romer Labs RapidChek®Listeria monocytogenes Test System for the Detection of L. monocytogenes on Selected Foods and Environmental Surfaces

2018 ◽  
Vol 101 (5) ◽  
pp. 1490-1507
Author(s):  
Gregory Juck ◽  
Verapaz Gonzalez ◽  
Ann-Christine Olsson Allen ◽  
Meredith Sutzko ◽  
Kody Seward ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Reference Method ◽  
Test System ◽  
Probability Of Detection ◽  
Department Of Agriculture ◽  
Detection Analysis ◽  
Media System ◽  
Reference Methods ◽  
Environmental Surfaces ◽  
Inspection Service

Abstract The Romer Labs RapidChek®Listeria monocytogenes test system (Performance Tested Method 011805) was validated against the U.S. Department of Agriculture-Food Safety and Inspection Service Microbiology Laboratory Guidebook (USDA-FSIS/MLG), U.S. Food and Drug Association Bacteriological Analytical Manual (FDA/BAM), and AOAC Official Methods of Analysis (AOAC/OMA) cultural reference methods for the detection of L. monocytogenes on selected foods including hot dogs, frozen cooked breaded chicken, frozen cooked shrimp, cured ham, and ice cream, and environmental surfaces including stainless steel and plastic in an unpaired study design. The RapidChek method uses a proprietary enrichment media system, a 44–48 h enrichment at 30 ± 1°C, and detects L. monocytogenes on an immunochromatographic lateral flow device within 10 min. Different L. monocytogenes strains were used to spike each of the matrixes. Samples were confirmed based on the reference method confirmations and an alternate confirmation method. A total of 140 low-level spiked samples were tested by the RapidChek method after enrichment for 44–48 h in parallel with the cultural reference method. There were 88 RapidChek presumptive positives. One of the presumptive positives was not confirmed culturally. Additionally, one of the culturally confirmed samples did not exhibit a presumptive positive. No difference between the alternate confirmation method and reference confirmation method was observed. The respective cultural reference methods (USDA-FSIS/MLG, FDA/BAM, and AOAC/OMA) produced a total of 63 confirmed positive results. Nonspiked samples from all foods were reported as negative for L. monocytogenes by all methods. Probability of detection analysis demonstrated no significant differences in the number of positive samples detected by the RapidChek method and the respective cultural reference method.

scholarly journals Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates

2008 ◽  
Vol 50 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Cristiane Yumi Koga-Ito ◽  
Juliana Pereira Lyon ◽  
Maria Aparecida de Resende
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Test System ◽  
Test Method ◽  
In Vitro Susceptibility ◽  
Oral Candidosis ◽  
Broth Microdilution Method

Thirty Candida albicans isolated from oral candidosis patients and 30 C. albicans isolated from control individuals were studied. In vitro susceptibility tests were performed for amphotericin B, fluconazole, 5-flucytosine and itraconazole through the Clinical and Laboratorial Standards Institute (CLSI) reference method and E test system. The results obtained were analyzed and compared. MIC values were similar for the strains isolated from oral candidosis patients and control individuals. The agreement rate for the two methods was 66.67% for amphotericin B, 53.33% for fluconazole, 65% for flucytosine and 45% for itraconazole. According to our data, E test method could be an alternative to trial routine susceptibility testing due to its simplicity. However, it can not be considered a substitute for the CLSI reference method.

scholarly journals Evaluation of a Preclinical Blood Test for Scrapie in Sheep Using Immunocapillary Electrophoresis

2006 ◽  
Vol 89 (3) ◽  
pp. 720-727 ◽  
Author(s):  
Roy Jackman ◽  
David J Everest ◽  
Mary Jo Schmerr ◽  
Mohammed Khawaja ◽  
Pat Keep ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Prion Protein ◽  
Clinical Signs ◽  
Test System ◽  
Buffy Coat ◽  
Infected Sheep ◽  
Test Method ◽  
Peptide Sequence ◽  
Transmissible Spongiform Encephalopathies ◽  
Blood Samples

Abstract An analytical method is described for detection of endogenous disease-associated prion protein in the buffy coat fraction from the blood of sheep infected with scrapie. The method has been improved and evaluated for its performance in the preclinical diagnosis of ovine transmissible spongiform encephalopathies. The test system uses a protocol for sample preparation that includes extraction and concentration and a test method that uses a liquid-phase competitive immunoassay for prion protein. Antibodies directed to a peptide sequence at the C-terminus of the prion protein (PrP) and a fluorescein-labeled peptide conjugate are used in the assay. Free zone capillary electrophoresis with laser-induced fluorescence for detection is used to separate the antibody-bound fluorescently labeled peptide and free labeled peptide. In this assay, the PrP competes with the fluorescently labeled peptide for limited antibody binding sites, which results in a reduction of the peak representing the immunocomplex of the antibody bound to the fluorescently labeled peptide. When blood samples from scrapie-infected sheep aged 712 months and of the scrapie-susceptible PrP genotypes VRQ/VRQ and VRQ/ARQ were analyzed, the abnormal PrP was found in blood samples. These results correlated with the post-mortem diagnosis of scrapie. The sheep were preclinical and appeared normal at the time of testing but later died with clinical disease approximately 12 months after testing. In older animals, and those with clinical signs, a smaller percentage of animals tested positive. This study has demonstrated that this technology can be used as a sensitive, rapid preclinical test to detect the disease-associated PrP in the blood of scrapie-infected sheep. Improvements in the extraction protocol and capillary electrophoresis conditions will enhance the robustness of this test.

The Research of IC Test Based on LTX-77 Test System

2014 ◽  
Vol 496-500 ◽  
pp. 1176-1179
Author(s):  
Li Tan ◽  
Yu Fang
Keyword(s):  
Test Object ◽  
Test System ◽  
Test Method ◽  
Test Results ◽  
Analog Ic ◽  
Test Platform ◽  
Logic Functions ◽  
Actual Test ◽  
Ic Test

LTX-77 test system is a large IC test system that is used for various kinds of analog IC, digital IC and analog digital mixed IC. It can be used to test DC parameters, AC parameters and logic functions. In the paper, the IC test platform is LTX-77 test system. IC ADC0804 was tested as the test object. The test method of IC is described in the view of actual test. The test results show that the test system is convenient and accurate, which has important practical value for IC manufacturers and users.

Design on Hierarchical Testing System for Unmanned Ground Vehicles

2011 ◽  
Vol 346 ◽  
pp. 817-822 ◽  
Author(s):  
Xin Li ◽  
Guang Ming Xiong ◽  
Yang Sun ◽  
Shao Bin Wu ◽  
Jian Wei Gong ◽  
...  
Keyword(s):  
Evaluation System ◽  
Test System ◽  
Unmanned Vehicles ◽  
Intelligent Vehicles ◽  
Test Method ◽  
Unmanned Ground Vehicles ◽  
Ground Vehicles ◽  
Test Environment ◽  
Test And Evaluation ◽  
Comprehensive Test

The test system for technical abilities of unmanned vehicles is gradually developed from the single test to comprehensive test. The pre-established test and evaluation system can promote the development of unmanned ground vehicles. The 2009 Future Challenge: Intelligent Vehicles and Beyond (FC’09) pushed China's unmanned vehicles out of laboratories. This paper proposed to design a more scientific and comprehensive test system for future competitions to better guide and regulate the development of China's unmanned vehicles. According to the design idea of stage by stage and level by level, the hierarchical test content from simple to advanced, from local to overall is designed. Then the hierarchic test environment is established according to the levels of test content. The test method based on multi-platform and multi-sensor is put forward to ensure the accuracy of test results. The testing criterion framework is set up to regulate future unmanned vehicle contests and to assess the unmanned vehicles scientifically and accurately.

scholarly journals Viscous Fingering of Miscible Liquids in Porous and Swellable Media for Rapid Diagnostic Tests

2018 ◽  
Vol 5 (4) ◽  
pp. 94
Author(s):  
Holly Clingan ◽  
Devon Rusk ◽  
Kathryn Smith ◽  
Antonio Garcia
Keyword(s):  
Saliva Sample ◽  
Fluid Motion ◽  
Test Strip ◽  
Viscous Fingering ◽  
Test Method ◽  
Colorimetric Test ◽  
Significant Movement ◽  
Miscible Liquids ◽  
Korteweg Stresses ◽  
Kinetics Of

In lateral flow and colorimetric test strip diagnostics, the effects of capillary action and diffusion on speed and sensitivity have been well studied. However, another form of fluid motion can be generated due to stresses and instabilities generated in pores when two miscible liquids with different densities and viscosities come into contact. This study explored how a swellable test pad can be deployed for measuring urea in saliva by partially prefilling the pad with a miscible solution of greater viscosity and density. The resultant Korteweg stresses and viscous fingering patterns were analyzed using solutions with added food color through video analysis and image processing. Image analysis was simplified using the saturation channel after converting RGB image sequences to HSB. The kinetics of liquid mixing agreed with capillary displacement results for miscible liquids undergoing movement from Korteweg stresses. After capillary filling, there was significant movement of liquid due to these fluidic effects, which led to mixing of the saliva sample with an enzyme test solution. Owing to the simplicity and speed of this test method, urea can be analyzed with an electronic nose over a useful range for detecting salivary urea concentration for rapid and early detection of dehydration.

Plant Constituents Interfering with Human Sex Hormone-Binding Globulin. Evaluation of a Test Method and Its Application to Urtica dioica Root Extracts

1995 ◽  
Vol 50 (1-2) ◽  
pp. 98-104 ◽  
Author(s):  
Dietmar Ganßer ◽  
Gerhard Spiteller
Keyword(s):  
Test System ◽  
Binding Activity ◽  
Urtica Dioica ◽  
Sex Hormone ◽  
Test Method ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Root Extracts ◽  
Plant Constituents ◽  
Octadecenoic Acids

Abstract A test system is described, which allows the search for compounds interfering with human sex hormone-binding globulin (SHBG) even in complex plant extracts. The method has been evaluated and applied to Urtica dioica root extracts. The lignan secoisolariciresinol (5) as well as a mixture of isomeric (11 E)-9,10,13-trihydroxy-11-octadecenoic and (10 E)-9 ,12,13-trihydroxy-10-octadecenoic acids (3 and 4, resp.) were demonstrated to reduce binding activity of human SHBG. Methylation of the mixture of 3 and 4 increased its activity about 10-fold.

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